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  • ‹ Back to RNA-seq
  • Stranded libraries from picogram-input total RNA (v3)
  • Automation-friendly, all-in-one cDNA synthesis and library prep
  • All-in-one cDNA synthesis and library prep from ultra-low RNA inputs
  • Stranded libraries from picogram-input total RNA (v2)
  • Stranded libraries from FFPE inputs (v2)
  • Stranded libraries from 100 ng - 1 ug total RNA
  • Stranded libraries from 100 pg-100 ng total RNA
  • Stranded libraries from picogram-input total RNA (v1)
  • Stranded RNA-seq competitor kit comparison
  • Nonstranded libraries from FFPE inputs
  • Sensitive capture of full-length transcript information with targeted RNA-seq
Low-input SMARTer stranded RNA-seq kits
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Home › Learning centers › Next-generation sequencing › Technical notes › RNA-seq › Stranded RNA-seq competitor kit comparison

Technical notes

  • Single-cell RNA- and DNA-seq
    • All-in-one cDNA synthesis and library prep from single cells
    • Highest sensitivity for single-cell mRNA-seq
    • Stranded libraries from single cells
    • Streamlined single-cell mRNA-seq
    • Full-length mRNA libraries from single cells (SMART-Seq v4)
    • 3' mRNA libraries from single cells (SMART-Seq v4 3' DE Kit)
    • Full-length mRNA libraries from single cells for Fluidigm C1 (SMART-Seq v4)
    • Full-length single-cell library method comparison
    • High-resolution CNV detection using PicoPLEX Gold DNA-Seq
    • Next-gen WGA method for CNV and SNV detection from single cells
  • RNA-seq
    • Stranded libraries from picogram-input total RNA (v3)
    • Automation-friendly, all-in-one cDNA synthesis and library prep
    • All-in-one cDNA synthesis and library prep from ultra-low RNA inputs
    • Stranded libraries from picogram-input total RNA (v2)
    • Stranded libraries from FFPE inputs (v2)
    • Stranded libraries from 100 ng - 1 ug total RNA
    • Stranded libraries from 100 pg-100 ng total RNA
    • Stranded libraries from picogram-input total RNA (v1)
    • Stranded RNA-seq competitor kit comparison
    • Nonstranded libraries from FFPE inputs
    • Sensitive capture of full-length transcript information with targeted RNA-seq
  • DNA-seq
    • Comparing ThruPLEX HV PLUS to Kapa and NEBNext
    • Improvements to ThruPLEX HV
    • ThruPLEX HV outperforms NEBNext Ultra II
    • Accurate detection of low-frequency variants using molecular tags
    • Streamlined DNA-seq from challenging samples
    • Low cell number ChIP-seq using SMARTer ThruPLEX
    • Cell-free DNA sequencing
    • Sequencing analysis of low-frequency mutations in cfDNA
    • DNA-seq from FFPE samples
    • Low-input whole-exome sequencing
    • Tag-seq variant detection
    • Low-volume DNA shearing for SMARTer ThruPLEX library prep
  • Immune profiling
    • BCR repertoire profiling from human samples (bulk)
    • Improved TCR repertoire profiling from human samples (bulk)
    • TCR repertoire profiling from human samples (single cells)
    • TCR repertoire profiling from human samples (bulk)
    • TCR repertoire profiling from mouse samples (bulk)
    • BCR repertoire profiling from mouse samples (bulk)
  • Epigenetics and smRNA-seq
    • Full-length small RNA libraries
    • ChIP-seq libraries for transcription factor analysis
    • ChIP-seq libraries from ssDNA
    • Methylated DNA-seq
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Low-input SMARTer stranded RNA-seq kits
RiboGone - Mammalian product page Visit the RiboGone - Mammalian product page
Tech Note

Comparing the performance of RNA-seq kits for inputs of varying sample type and quality

Data kindly provided by Sri Ganesh Jammula, Ph.D., Karin Johanna Ferrari, Ph.D., Andrea Scelfo, Ph.D., Alessandra Rossi, Ph.D., and Diego Pasini, Ph.D., Department of Experimental Oncology, European Institute of Oncology

  • In a customer-conducted performance analysis with standard RNA control samples, Takara Bio and Illumina RNA-seq kits yielded consistent and comparable sequencing metrics.
  • Strong correlations were also observed between kits for data generated from varying input amounts of high-quality and partially degraded RNA.
  • According to customer feedback, Takara Bio's SMARTer Stranded RNA-Seq Kit performed better than Illumina's TruSeq® RNA Sample Preparation Kit v2 for both intact and partially degraded mouse RNA inputs.
  • The SMARTer Stranded RNA-Seq Kit features a fast and easy workflow of ~4 hours, which can quickly generate results from even the most challenging samples.
Introduction Results Summary Methods

Introduction  

RNA-seq has become an essential tool for analyzing transcriptional differences between cell populations and individual cells. To empower accurate transcriptome analyses in a range of biomedical research applications—including those in which RNA inputs are low or compromised—several protocols and advancements have been made for RNA-seq. This technical note features performance data generated for human and mouse RNA inputs of varying quality using four commercially available solutions for RNA-seq: the TruSeq Stranded Total RNA with Ribo-Zero™ Human/Mouse/Rat kit (Illumina), the TruSeq RNA Sample Preparation Kit v2 (Illumina), the SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Takara Bio), and the SMARTer Stranded RNA-Seq Kit (Takara Bio; RiboGone rRNA depletion technology is included in the high-input kit, and was used in conjunction with the SMARTer Stranded RNA-Seq Kit). The experiments were performed at the European Institute of Oncology and the Italian Institute of Technology Center for Genomic Science (both in Milan, Italy) under the direction of Dr. Diego Pasini, and by staff from the Takara Bio R&D department. The results indicate that the Takara Bio and Illumina kits yield consistent and comparable sequencing metrics for standard human RNA control samples, high-quality mouse embryonic stem cell RNA, and partially degraded mouse intestinal RNA. Additionally, analysis of partially degraded mouse intestinal RNA samples using the SMARTer Stranded RNA-Seq Kit revealed divergent expression levels for genes known to be differentially regulated between various intestinal cell types, evidence of the sensitivity afforded by this kit.

Results  

Comparable sequencing metrics from experiments using standard RNA inputs

To compare the performance of the SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian and the TruSeq Stranded Total RNA with Ribo-Zero Human/Mouse/Rat, each kit was used to generate a sequencing library from 400 ng of Microarray Quality Control Consortium (MAQC) human total RNA-Human Universal Reference RNA (HURR) or Human Brain Reference RNA (HBRR)-spiked with External RNA Controls Consortium (ERCC) synthetic RNA, followed by sequencing and data analysis. The two methods yielded comparable sequencing metrics (Table 1) in terms of rRNA depletion, exon/intron mapping, and the number of genes identified at thresholds of 0.1 or 1 RPKMs (Reads Per Kilobase of Exon Per Million Fragments Mapped). Minor differences were observed between the data sets for various sequencing metrics, presumably because while both kits include similar cDNA-synthesis, rRNA-removal, and library-preparation steps, they employ different proprietary technologies.

Sequencing metrics for libraries generated from 400 ng total RNA inputs
Human total RNA type Universal (HURR) Brain (HBRR) Universal (HURR) Brain (HBRR)
PCR cycles 12 15
Library prep kit used SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian with RiboGone - Mammalian TruSeq Stranded Total RNA with Ribo-Zero Human/Mouse/Rat
Number of reads (millions) 8.5 (paired-end) 7.0 (paired-end)
Percentage of reads (%)
After trimming 97.8 94.4 99.4 99.3
Mapped to rRNA 0.28 5.36 0.6 0.24
Mapped to mitochondria 13 15 22 14
Mapped to genome 87 87 95 95
Mapped to exons 45 50 54 41
Mapped to introns 45 41 40 49
Mapped to intergenic regions 11 9.5 6.4 9.2
Number of genes with at least 0.1 RPKM 25,465 24,026 24,578 24,587
Number of genes with at least 1.0 RPKM 15,672 14,960 15,975 15,624
Percentage of duplicates (%) 24 23 23 13
Biological strandedness (%) 98.4 96.4 99.4 98.9
ERCC strandedness (%) 99.3 98.8 99.9 100
Percentage of reads mapped to ERCC (%) 0.54 0.54 1 0.9


Table 1. Sequence alignment metrics.
400 ng of Human Universal Reference RNA (HURR) or Human Brain Reference RNA (HBRR) spiked with ERCC RNA was processed with each kit. Alignment data is displayed for each library, with the percentage of reads that mapped to rRNA, exons, introns, intergenic regions, and the correct strand, etc., as defined by Picard analysis.

In addition to yielding similar alignment metrics, RNA-seq data generated from HURR and HBRR samples with each kit correlated strongly (R2 >0.8) with MAQC-generated data (Figure 1). This concordance speaks to the robustness and comparable performance of both kits.

Correlation between RNA-seq data and MAQC data.

Figure 1. Correlation between RNA-seq data and MAQC data. RNA-seq libraries were independently generated from 400 ng of HURR or HBRR using each kit. Each scatter plot shows the log2 of the ratio of HURR/HBRR RPKMs graphed against the log2 of the ratio of HURR/HBRR expression data derived from qPCR Taqman probes. Panel A. Correlation for data generated with the SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian. Panel B. Correlation for data generated with the TruSeq Stranded Total RNA with Ribo-Zero Human/Mouse/Rat kit.

Testing the efficiencies of two different library preparation methods using high-quality RNA inputs

High-quality RNA isolated from mouse embryonic stem cells was used to compare the efficiencies of the SMARTer Stranded RNA-Seq Kit, and the TruSeq RNA Sample Preparation Kit v2 (Figure 2). Data Set A was obtained from a sequencing library prepared using the Illumina kit with an input consisting of poly(A)-enriched mRNA derived from 1 µg of total RNA. Data Sets B and C were obtained from sequencing libraries generated from 10 ng and 100 ng, respectively, of total RNA using the Takara Bio kit. In summary, mRNA expression data for both libraries generated using the Takara Bio kit correlated strongly with data from the library generated using the Illumina kit (R2 >0.9 for each comparison), and the kits yielded comparable transcript mapping results. In particular, there was considerable overlap for the 25% most highly expressed transcripts identified in each data set, as indicated by the Venn diagram. These results suggest that the Takara Bio kit may offer greater efficiency than the Illumina kit, in that the former can generate comparable sequencing results from a lower input amount.

Assessing the efficiency of the SMARTer Stranded RNA-Seq Kit for sequencing high-quality input RNA.

Figure 2. Assessing the efficiency of the SMARTer Stranded RNA-Seq Kit for sequencing high-quality input RNA. RNA-seq libraries were generated from either total or poly(A)-enriched mouse embryonic stem cell RNA, using either the SMARTer kit (paired with RiboGone - Mammalian), or the Illumina TruSeq RNA Sample Preparation Kit v2. Panel A. Correlation of normalized mRNA expression data (log-transformed RPKMs) for the Illumina TruSeq library (Data Set A) vs. the Takara Bio SMARTer library (10 ng total RNA input, Data Set B). Data points are color-coded according to relative expression levels in the TruSeq dataset (red: 75th–100th percentile, green: 50th–75th percentile, blue: 25th–50th percentile, purple: 0–25th percentile). The correlation coefficient (R2) for the two data sets is indicated in the top left corner of the graph. Panel B. Correlation of normalized mRNA expression data for the Illumina TruSeq library (Data Set A) vs. the Takara Bio SMARTer library (100 ng total RNA input, Data Set C). Data points are color coded as in Panel A. The correlation coefficient (R2) for the two data sets is indicated in the top left corner of the graph. Panel C. Venn diagram demonstrating overlap for the 25% most highly expressed transcripts in each dataset.

Data reproducibility across varying input amounts

Expression data for the two SMARTer libraries generated from 10 ng or 100 ng inputs of high-quality mouse embryonic stem cell RNA were compared directly (Figure 3). The strong correlation observed (R2 = 0.971) suggests that the SMARTer Stranded RNA-Seq Kit generates consistent results for varying input amounts of total RNA. In addition, the strand specificity of sequencing reads was analyzed by measuring the frequencies with which the reads indicated a gene's annotated strand of origin ("specific strand") vs. the complementary strand ("opposite strand"). The results confirmed that most reads mapped to the annotated strand regardless of the input amount (100 ng vs. 10 ng), suggesting that sequencing libraries produced with the SMARTer Stranded Total RNA-Seq Kit reliably capture strand-of-origin information.

Data reproducibility across varying input amounts.

Figure 3. Data reproducibility across varying input amounts. Data sets for RNA-seq libraries generated from 10 ng or 100 ng of mouse embryonic stem cell total RNA using the SMARTer Stranded RNA-Seq Kit were compared. Panel A. Correlation of normalized mRNA expression data (log-transformed RPKMs) for 10 ng input (Data Set B) vs. 100 ng input (Data Set C). The correlation coefficient (R2) for the two data sets is indicated in the top left corner of the graph. Panel B. Strand-specificity data for reads mapped to annotated genes in each data set. The boxplots summarize the normalized quantities of reads that identified the annotated strand of origin ("specific strand") vs. the corresponding complementary strand ("opposite strand") for each gene, for both data sets.

Testing the efficiencies of two different library preparation methods using partially degraded RNA inputs

RNA isolated from partially degraded mouse intestinal tissue was used to compare the efficiencies of the Illumina and Takara Bio kits in processing compromised samples (Figure 4). Data Set D was obtained from a sequencing library prepared using the Illumina TruSeq RNA Sample Preparation Kit v2. The input for this data set was poly(A)-enriched mRNA derived from 1 µg of total RNA. Data Set E was obtained from a sequencing library generated from 100 ng of total RNA using the SMARTer Stranded RNA-Seq Kit paired with RiboGone - Mammalian. Expression data from the two libraries were strongly correlated (R2 = 0.948), and considerable overlap was observed for the 25% most highly expressed transcripts identified in each data set, as indicated by the Venn diagram. As with the above comparison involving high-quality mouse RNA, these results suggest that the Takara Bio kit may offer greater efficiency than the Illumina kit.

Assessing the efficiency of the SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian for sequencing of partially degraded input RNA.

Figure 4. Assessing the efficiency of the SMARTer Stranded RNA-Seq Kit for sequencing of partially degraded input RNA. RNA-seq libraries were generated from partially degraded mouse intestinal RNA using either the SMARTer kit, or the Illumina TruSeq RNA Sample Preparation Kit v2. Panel A. Correlation of normalized expression data (log-transformed RPKMs) for the Illumina TruSeq library (Data Set D) vs. the Takara Bio SMARTer library (Data Set E). Data points are color-coded according to relative expression levels in the TruSeq dataset (red: 75th-100th percentile, green: 50th-75th percentile, blue: 25th-50th percentile, purple: 0-25th percentile). The correlation coefficient (R2) for the two data sets is indicated in the top left corner of the graph. Panel B. Venn diagram demonstrating overlap for the 25% most highly expressed transcripts in each dataset.

Detecting tissue-specific differences in gene expression from partially degraded RNA inputs

To further test the capabilities of the SMARTer Stranded RNA-Seq Kit in processing partially degraded samples, sequencing libraries were generated from RNA inputs derived from the small intestine and colon (Data Sets E and F, respectively). While expression levels for many genes were correlated (Figure 5), the analysis also identified genes that were differentially expressed in the respective samples, and confirmed that much of this divergence was consistent with known transcriptomic differences between the small intestine and colon. As was the case for data generated from high-quality RNA inputs, analysis of strand specificity for the small intestine and colon data sets confirmed that the vast majority of sequencing reads correctly indicated the annotated strand of origin for the corresponding genes to which they mapped. These results demonstrate that the SMARTer Stranded RNA-Seq Kit successfully preserves strand-of-origin information for compromised RNA inputs.

Observing tissue-specific differences in gene expression and obtaining strand-of-origin information from partially degraded RNA inputs.

Figure 5. Observing tissue-specific differences in gene expression and obtaining strand-of-origin information from partially degraded RNA inputs. RNA-seq libraries were generated from 100 ng inputs of partially degraded RNA from mouse small intestine and colon (Data Sets E and F, respectively) using the SMARTer Stranded RNA-Seq Kit. Panel A. Correlation of normalized mRNA expression data (log-transformed RPKMs) for Data Set E vs. Data Set F. Differentially expressed genes attributed with elevated expression in the intestine and colon are indicated in red and blue, respectively. Panel B. Strand-specificity data for reads mapped to annotated genes in each data set. The boxplots summarize the normalized quantities of reads that identified the annotated strand of origin ("specific strand") vs. the corresponding complementary strand ("opposite strand") for each gene, for both data sets.

Summary  

A customer comparison of Takara Bio and Illumina RNA-seq library preparation kits indicated that they yield comparable sequencing results for a range of RNA inputs, including control human RNA, high-quality mouse RNA, and degraded mouse RNA. Included in the comparisons were the SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Takara Bio), the SMARTer Stranded RNA-Seq Kit (Takara Bio), the TruSeq Stranded Total RNA with Ribo-Zero Human/Mouse/Rat kit (Illumina), and the TruSeq RNA Sample Preparation Kit v2 (Illumina). Whereas library preparation with the Takara Bio and Illumina stranded kits involved depletion of rRNA from total RNA inputs, mRNA processed with the Illumina RNA Sample Preparation Kit v2 was poly(A)-enriched. Correspondingly, for the comparisons involving mouse RNA inputs, the input amount used with the Illumina TruSeq RNA Sample Preparation Kit v2 (1 µg) was much greater than the input amounts used with the Takara Bio kit (10 ng or 100 ng). According to the customer who conducted the study, the Takara Bio SMARTer Stranded RNA-Seq Kit performed better overall than the Illumina TruSeq RNA Sample Preparation Kit v2 for both high-quality and partially degraded inputs of mouse RNA (data not shown). Importantly, known differences in gene expression between tissues of the mouse intestine and colon were represented in sequencing libraries generated from partially degraded RNA samples using the Takara Bio kit. For both mouse inputs tested, the Takara Bio kit captured strand-of-origin information with a high degree of accuracy. Taken together, these data suggest that the SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian and the SMARTer Stranded Total RNA-Seq Kit provide more efficient and robust methods for sequencing library preparation compared to available alternatives.

Methods  

  • Kits used were the SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Cat. No. 634873), the SMARTer Stranded RNA-Seq Kit (Takara Bio, Cat. No. 634836), RiboGone - Mammalian (Takara Bio, Cat. No. 634846), the TruSeq Stranded Total RNA with Ribo-Zero Human/Mouse/Rat (Illumina, Cat. No. RS-122-2201), and the TruSeq RNA Sample Preparation Kit v2 (Illumina, Cat. No. RS-122-9001).
  • RNA inputs included Human Universal Reference RNA (Agilent, Cat. No. 740000), FirstChoice Human Brain Reference RNA (Thermo Fisher Scientific, Cat. No. AM6050), and ERCC Spike-In Mix (Thermo Fisher Scientific, Cat. No. 4456740).
  • All libraries in this study were sequenced with paired-end reads (1 x 50 bp) on an Illumina HiSeq™ 2000 instrument.
  • Sequencing data analysis was done using CLC Genomics Workbench.

Related Products

Cat. # Product Size Price License Quantity Details
634846 RiboGone™ - Mammalian 6 Rxns USD $360.00

License Statement

ID Number  
246 This product is protected by U.S. Patent 9,428,794, and additional pending US and foreign patents. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.

RiboGone - Mammalian allows for the specific removal of rRNA sequences (5S, 5.8S, 18S, and 28S), as well as mitochondrial rRNA sequences (12S), from human, mouse, or rat total RNA. This kit is designed for use with limited sample amounts (10–100 ng of total RNA), and works with high- or low-quality RNA. Samples processed using the RiboGone kit are ready for cDNA synthesis with random primers.

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Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis
RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Clontech) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Clontech), while retaining more noncoding reads.

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Sequencing data of RiboGone - Mammalian treated RNA samples highly correlate with MAQC qPCR data for the same reference RNA

Sequencing data of RiboGone - Mammalian treated RNA samples highly correlate with MAQC qPCR data for the same reference RNA
Sequencing data of RiboGone - Mammalian treated RNA samples highly correlate with MAQC qPCR data for the same reference RNA. The high level of correlation between the RNA-Seq and MAQC data (R=0.860) shows that depletion of rRNA with RiboGone does not interfere with detection of other genes by RNA-seq.

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Comparison of Human Universal Reference RNA and Human Brain Reference RNA sequencing alignment metrics for 10 ng of RNA treated with the RiboGone - Mammalian kit and the SMARTer Stranded RNA-Seq Kit show that RiboGone efficiently degrades rRNA from small total RNA samples

Comparison of Human Universal Reference RNA and Human Brain Reference RNA sequencing alignment metrics for 10 ng of RNA treated with the RiboGone - Mammalian kit and the SMARTer Stranded RNA-Seq Kit show that RiboGone efficiently degrades rRNA from small total RNA samples
Comparison of Human Universal Reference RNA and Human Brain Reference RNA sequencing alignment metrics for 10 ng of RNA treated with the RiboGone - Mammalian kit and the SMARTer Stranded RNA-Seq Kit show that RiboGone efficiently degrades rRNA from small total RNA samples.

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RiboGone - Mammalian is specifically designed to work with low input RNA samples containing between 10–100 ng of full-length or degraded total RNA

RiboGone - Mammalian is specifically designed to work with low input RNA samples containing between 10–100 ng of full-length or degraded total RNA
RiboGone - Mammalian is specifically designed to work with low input RNA samples containing between 10–100 ng of full-length or degraded total RNA. Samples processed using the RiboGone kit are ready for cDNA synthesis with any random-primed SMARTer RNA-Seq kit (including the SMARTer Stranded RNA-Seq Kit, the SMARTer Universal Low Input RNA Kit for Sequencing, and the SMARTer Universal Low Input RNA Library Prep Kit).

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634846: RiboGone - Mammalian

634846: RiboGone - Mammalian

Required Products

Cat. # Product Size Price License Quantity Details
634836 SMARTer® Stranded RNA-Seq Kit 12 Rxns USD $1027.00

The SMARTer Stranded RNA-Seq Kit includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform, starting from as little as 100 pg of polyA-purified or ribosomal RNA-depleted RNA. The kit consists of the SMARTer Stranded RNA-Seq Components, SeqAmp DNA Polymerase, and the llumina Indexing Primer Set (PCR primers for the amplification of indexed, paired-end Illumina-compatible sequencing libraries, which enable multiplexing of NGS library analysis).

The SMARTer Stranded RNA-Seq Kit utilizes our patented SMART (Switching Mechanism At 5' end of RNA Template) technology, coupled with PCR amplification, to generate Illumina-compatible libraries without the need for enzymatic clean-up or adapter ligations. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA.

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cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations
cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain polyA+ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit).

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The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels
The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels. Scatter plots of expression (FPKM) for cDNA libraries prepared from 100 ng and 100 pg of human brain polyA+ RNA show a high correlation, suggesting consistency across input levels. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

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Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations
Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations. Comparisons of pairs of cDNA library replicas created from 100 ng and 100 pg of input RNA show high reproducibility across a wide range of input RNA. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

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Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments
Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments.

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High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis
High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis. Reads mapping to the ERCC data set from the previous experiments were pooled together and the FPKM was plotted against relative transcript abundance. The complete set of 92 ERCC transcripts was identified, with a slope of 0.934 and R2 of 0.9725. Axes are plotted on a log2 scale.

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Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads
Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina® HiSeq® Platform with 2 x 100 bp paired end reads. Short, overlapping reads originating from different strands of the genomic DNA were distinguished from each other, enabling quantitative expression analysis and accurate genome annotation. 99% of RNA-Seq reads mapped to the correct strand.

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Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit
Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit. Panel A. RNA-seq reads from the Human Brain Poly A+ RNA cDNA library were mapped against the human genome. The SMARTer Stranded method allowed assignment of sequencing reads to the correct gene in the case of overlapping PHC1 and M6PR transcripts. Panel B. Strand-specific coverage of the CDR1 locus. Nearly all reads are antisense to the annotated transcript, a finding independently reported elsewhere (Hansen, T. B. et al. (2011) EMBO J. 30(21):4414–4422). Panel C. Comparison of CDR1 gene counts obtained using either a strand-agnostic or strand-aware method.

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RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis
RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Clontech) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Clontech), while retaining more noncoding reads.
634837 SMARTer® Stranded RNA-Seq Kit 24 Rxns USD $1850.00

The SMARTer Stranded RNA-Seq Kit includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform, starting from as little as 100 pg of polyA-purified or ribosomal RNA-depleted RNA. The kit consists of the SMARTer Stranded RNA-Seq Components, SeqAmp DNA Polymerase, and the llumina Indexing Primer Set (PCR primers for the amplification of indexed, paired-end Illumina-compatible sequencing libraries, which enable multiplexing of NGS library analysis).

The SMARTer Stranded RNA-Seq Kit utilizes our patented SMART (Switching Mechanism At 5' end of RNA Template) technology, coupled with PCR amplification, to generate Illumina-compatible libraries without the need for enzymatic clean-up or adapter ligations. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA.

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cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations
cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain polyA+ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit).

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The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels
The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels. Scatter plots of expression (FPKM) for cDNA libraries prepared from 100 ng and 100 pg of human brain polyA+ RNA show a high correlation, suggesting consistency across input levels. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

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Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations
Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations. Comparisons of pairs of cDNA library replicas created from 100 ng and 100 pg of input RNA show high reproducibility across a wide range of input RNA. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

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Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments
Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments.

Back

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis
High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis. Reads mapping to the ERCC data set from the previous experiments were pooled together and the FPKM was plotted against relative transcript abundance. The complete set of 92 ERCC transcripts was identified, with a slope of 0.934 and R2 of 0.9725. Axes are plotted on a log2 scale.

Back

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads
Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina® HiSeq® Platform with 2 x 100 bp paired end reads. Short, overlapping reads originating from different strands of the genomic DNA were distinguished from each other, enabling quantitative expression analysis and accurate genome annotation. 99% of RNA-Seq reads mapped to the correct strand.

Back

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit
Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit. Panel A. RNA-seq reads from the Human Brain Poly A+ RNA cDNA library were mapped against the human genome. The SMARTer Stranded method allowed assignment of sequencing reads to the correct gene in the case of overlapping PHC1 and M6PR transcripts. Panel B. Strand-specific coverage of the CDR1 locus. Nearly all reads are antisense to the annotated transcript, a finding independently reported elsewhere (Hansen, T. B. et al. (2011) EMBO J. 30(21):4414–4422). Panel C. Comparison of CDR1 gene counts obtained using either a strand-agnostic or strand-aware method.

Back

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis
RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Clontech) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Clontech), while retaining more noncoding reads.
634838 SMARTer® Stranded RNA-Seq Kit 48 Rxns USD $3125.00

The SMARTer Stranded RNA-Seq Kit includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform, starting from as little as 100 pg of polyA-purified or ribosomal RNA-depleted RNA. The kit consists of the SMARTer Stranded RNA-Seq Components, SeqAmp DNA Polymerase, and the llumina Indexing Primer Set (PCR primers for the amplification of indexed, paired-end Illumina-compatible sequencing libraries, which enable multiplexing of NGS library analysis).

The SMARTer Stranded RNA-Seq Kit utilizes our patented SMART (Switching Mechanism At 5' end of RNA Template) technology, coupled with PCR amplification, to generate Illumina-compatible libraries without the need for enzymatic clean-up or adapter ligations. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA.

Documents Components You May Also Like Image Data Resources

Back

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations
cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain polyA+ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit).

Back

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels
The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels. Scatter plots of expression (FPKM) for cDNA libraries prepared from 100 ng and 100 pg of human brain polyA+ RNA show a high correlation, suggesting consistency across input levels. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

Back

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations
Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations. Comparisons of pairs of cDNA library replicas created from 100 ng and 100 pg of input RNA show high reproducibility across a wide range of input RNA. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

Back

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments
Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments.

Back

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis
High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis. Reads mapping to the ERCC data set from the previous experiments were pooled together and the FPKM was plotted against relative transcript abundance. The complete set of 92 ERCC transcripts was identified, with a slope of 0.934 and R2 of 0.9725. Axes are plotted on a log2 scale.

Back

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads
Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina® HiSeq® Platform with 2 x 100 bp paired end reads. Short, overlapping reads originating from different strands of the genomic DNA were distinguished from each other, enabling quantitative expression analysis and accurate genome annotation. 99% of RNA-Seq reads mapped to the correct strand.

Back

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit
Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit. Panel A. RNA-seq reads from the Human Brain Poly A+ RNA cDNA library were mapped against the human genome. The SMARTer Stranded method allowed assignment of sequencing reads to the correct gene in the case of overlapping PHC1 and M6PR transcripts. Panel B. Strand-specific coverage of the CDR1 locus. Nearly all reads are antisense to the annotated transcript, a finding independently reported elsewhere (Hansen, T. B. et al. (2011) EMBO J. 30(21):4414–4422). Panel C. Comparison of CDR1 gene counts obtained using either a strand-agnostic or strand-aware method.

Back

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis
RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Clontech) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Clontech), while retaining more noncoding reads.
634839 SMARTer® Stranded RNA-Seq Kit 96 Rxns USD $4024.00

The SMARTer Stranded RNA-Seq Kit includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform, starting from as little as 100 pg of polyA-purified or ribosomal RNA-depleted RNA. The kit consists of the SMARTer Stranded RNA-Seq Components, SeqAmp DNA Polymerase, and the llumina Indexing Primer Set (PCR primers for the amplification of indexed, paired-end Illumina-compatible sequencing libraries, which enable multiplexing of NGS library analysis).

The SMARTer Stranded RNA-Seq Kit utilizes our patented SMART (Switching Mechanism At 5' end of RNA Template) technology, coupled with PCR amplification, to generate Illumina-compatible libraries without the need for enzymatic clean-up or adapter ligations. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA.

Documents Components You May Also Like Image Data Resources

Back

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations
cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain polyA+ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit).

Back

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels
The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels. Scatter plots of expression (FPKM) for cDNA libraries prepared from 100 ng and 100 pg of human brain polyA+ RNA show a high correlation, suggesting consistency across input levels. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

Back

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations
Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations. Comparisons of pairs of cDNA library replicas created from 100 ng and 100 pg of input RNA show high reproducibility across a wide range of input RNA. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

Back

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments
Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments.

Back

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis
High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis. Reads mapping to the ERCC data set from the previous experiments were pooled together and the FPKM was plotted against relative transcript abundance. The complete set of 92 ERCC transcripts was identified, with a slope of 0.934 and R2 of 0.9725. Axes are plotted on a log2 scale.

Back

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads
Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina® HiSeq® Platform with 2 x 100 bp paired end reads. Short, overlapping reads originating from different strands of the genomic DNA were distinguished from each other, enabling quantitative expression analysis and accurate genome annotation. 99% of RNA-Seq reads mapped to the correct strand.

Back

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit
Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit. Panel A. RNA-seq reads from the Human Brain Poly A+ RNA cDNA library were mapped against the human genome. The SMARTer Stranded method allowed assignment of sequencing reads to the correct gene in the case of overlapping PHC1 and M6PR transcripts. Panel B. Strand-specific coverage of the CDR1 locus. Nearly all reads are antisense to the annotated transcript, a finding independently reported elsewhere (Hansen, T. B. et al. (2011) EMBO J. 30(21):4414–4422). Panel C. Comparison of CDR1 gene counts obtained using either a strand-agnostic or strand-aware method.

Back

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis
RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Clontech) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Clontech), while retaining more noncoding reads.
634847 RiboGone™ - Mammalian 24 Rxns USD $1260.00

License Statement

ID Number  
246 This product is protected by U.S. Patent 9,428,794, and additional pending US and foreign patents. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.

RiboGone - Mammalian allows for the specific removal of rRNA sequences (5S, 5.8S, 18S, and 28S), as well as mitochondrial rRNA sequences (12S), from human, mouse, or rat total RNA. This kit is designed for use with limited sample amounts (10–100 ng of total RNA), and works with high- or low-quality RNA. Samples processed using the RiboGone kit are ready for cDNA synthesis with random primers.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis
RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Clontech) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Clontech), while retaining more noncoding reads.

Back

Sequencing data of RiboGone - Mammalian treated RNA samples highly correlate with MAQC qPCR data for the same reference RNA

Sequencing data of RiboGone - Mammalian treated RNA samples highly correlate with MAQC qPCR data for the same reference RNA
Sequencing data of RiboGone - Mammalian treated RNA samples highly correlate with MAQC qPCR data for the same reference RNA. The high level of correlation between the RNA-Seq and MAQC data (R=0.860) shows that depletion of rRNA with RiboGone does not interfere with detection of other genes by RNA-seq.

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Comparison of Human Universal Reference RNA and Human Brain Reference RNA sequencing alignment metrics for 10 ng of RNA treated with the RiboGone - Mammalian kit and the SMARTer Stranded RNA-Seq Kit show that RiboGone efficiently degrades rRNA from small total RNA samples

Comparison of Human Universal Reference RNA and Human Brain Reference RNA sequencing alignment metrics for 10 ng of RNA treated with the RiboGone - Mammalian kit and the SMARTer Stranded RNA-Seq Kit show that RiboGone efficiently degrades rRNA from small total RNA samples
Comparison of Human Universal Reference RNA and Human Brain Reference RNA sequencing alignment metrics for 10 ng of RNA treated with the RiboGone - Mammalian kit and the SMARTer Stranded RNA-Seq Kit show that RiboGone efficiently degrades rRNA from small total RNA samples.

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RiboGone - Mammalian is specifically designed to work with low input RNA samples containing between 10–100 ng of full-length or degraded total RNA

RiboGone - Mammalian is specifically designed to work with low input RNA samples containing between 10–100 ng of full-length or degraded total RNA
RiboGone - Mammalian is specifically designed to work with low input RNA samples containing between 10–100 ng of full-length or degraded total RNA. Samples processed using the RiboGone kit are ready for cDNA synthesis with any random-primed SMARTer RNA-Seq kit (including the SMARTer Stranded RNA-Seq Kit, the SMARTer Universal Low Input RNA Kit for Sequencing, and the SMARTer Universal Low Input RNA Library Prep Kit).

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634847: RiboGone - Mammalian

634847: RiboGone - Mammalian

Required Products

Cat. # Product Size Price License Quantity Details
634836 SMARTer® Stranded RNA-Seq Kit 12 Rxns USD $1027.00

The SMARTer Stranded RNA-Seq Kit includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform, starting from as little as 100 pg of polyA-purified or ribosomal RNA-depleted RNA. The kit consists of the SMARTer Stranded RNA-Seq Components, SeqAmp DNA Polymerase, and the llumina Indexing Primer Set (PCR primers for the amplification of indexed, paired-end Illumina-compatible sequencing libraries, which enable multiplexing of NGS library analysis).

The SMARTer Stranded RNA-Seq Kit utilizes our patented SMART (Switching Mechanism At 5' end of RNA Template) technology, coupled with PCR amplification, to generate Illumina-compatible libraries without the need for enzymatic clean-up or adapter ligations. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA.

Documents Components You May Also Like Image Data Resources

Back

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations
cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain polyA+ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit).

Back

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels
The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels. Scatter plots of expression (FPKM) for cDNA libraries prepared from 100 ng and 100 pg of human brain polyA+ RNA show a high correlation, suggesting consistency across input levels. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

Back

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations
Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations. Comparisons of pairs of cDNA library replicas created from 100 ng and 100 pg of input RNA show high reproducibility across a wide range of input RNA. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

Back

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments
Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments.

Back

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis
High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis. Reads mapping to the ERCC data set from the previous experiments were pooled together and the FPKM was plotted against relative transcript abundance. The complete set of 92 ERCC transcripts was identified, with a slope of 0.934 and R2 of 0.9725. Axes are plotted on a log2 scale.

Back

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads
Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina® HiSeq® Platform with 2 x 100 bp paired end reads. Short, overlapping reads originating from different strands of the genomic DNA were distinguished from each other, enabling quantitative expression analysis and accurate genome annotation. 99% of RNA-Seq reads mapped to the correct strand.

Back

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit
Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit. Panel A. RNA-seq reads from the Human Brain Poly A+ RNA cDNA library were mapped against the human genome. The SMARTer Stranded method allowed assignment of sequencing reads to the correct gene in the case of overlapping PHC1 and M6PR transcripts. Panel B. Strand-specific coverage of the CDR1 locus. Nearly all reads are antisense to the annotated transcript, a finding independently reported elsewhere (Hansen, T. B. et al. (2011) EMBO J. 30(21):4414–4422). Panel C. Comparison of CDR1 gene counts obtained using either a strand-agnostic or strand-aware method.

Back

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis
RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Clontech) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Clontech), while retaining more noncoding reads.
634837 SMARTer® Stranded RNA-Seq Kit 24 Rxns USD $1850.00

The SMARTer Stranded RNA-Seq Kit includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform, starting from as little as 100 pg of polyA-purified or ribosomal RNA-depleted RNA. The kit consists of the SMARTer Stranded RNA-Seq Components, SeqAmp DNA Polymerase, and the llumina Indexing Primer Set (PCR primers for the amplification of indexed, paired-end Illumina-compatible sequencing libraries, which enable multiplexing of NGS library analysis).

The SMARTer Stranded RNA-Seq Kit utilizes our patented SMART (Switching Mechanism At 5' end of RNA Template) technology, coupled with PCR amplification, to generate Illumina-compatible libraries without the need for enzymatic clean-up or adapter ligations. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA.

Documents Components You May Also Like Image Data Resources

Back

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations
cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain polyA+ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit).

Back

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels
The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels. Scatter plots of expression (FPKM) for cDNA libraries prepared from 100 ng and 100 pg of human brain polyA+ RNA show a high correlation, suggesting consistency across input levels. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

Back

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations
Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations. Comparisons of pairs of cDNA library replicas created from 100 ng and 100 pg of input RNA show high reproducibility across a wide range of input RNA. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

Back

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments
Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments.

Back

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis
High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis. Reads mapping to the ERCC data set from the previous experiments were pooled together and the FPKM was plotted against relative transcript abundance. The complete set of 92 ERCC transcripts was identified, with a slope of 0.934 and R2 of 0.9725. Axes are plotted on a log2 scale.

Back

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads
Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina® HiSeq® Platform with 2 x 100 bp paired end reads. Short, overlapping reads originating from different strands of the genomic DNA were distinguished from each other, enabling quantitative expression analysis and accurate genome annotation. 99% of RNA-Seq reads mapped to the correct strand.

Back

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit
Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit. Panel A. RNA-seq reads from the Human Brain Poly A+ RNA cDNA library were mapped against the human genome. The SMARTer Stranded method allowed assignment of sequencing reads to the correct gene in the case of overlapping PHC1 and M6PR transcripts. Panel B. Strand-specific coverage of the CDR1 locus. Nearly all reads are antisense to the annotated transcript, a finding independently reported elsewhere (Hansen, T. B. et al. (2011) EMBO J. 30(21):4414–4422). Panel C. Comparison of CDR1 gene counts obtained using either a strand-agnostic or strand-aware method.

Back

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis
RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Clontech) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Clontech), while retaining more noncoding reads.
634838 SMARTer® Stranded RNA-Seq Kit 48 Rxns USD $3125.00

The SMARTer Stranded RNA-Seq Kit includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform, starting from as little as 100 pg of polyA-purified or ribosomal RNA-depleted RNA. The kit consists of the SMARTer Stranded RNA-Seq Components, SeqAmp DNA Polymerase, and the llumina Indexing Primer Set (PCR primers for the amplification of indexed, paired-end Illumina-compatible sequencing libraries, which enable multiplexing of NGS library analysis).

The SMARTer Stranded RNA-Seq Kit utilizes our patented SMART (Switching Mechanism At 5' end of RNA Template) technology, coupled with PCR amplification, to generate Illumina-compatible libraries without the need for enzymatic clean-up or adapter ligations. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA.

Documents Components You May Also Like Image Data Resources

Back

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations
cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain polyA+ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit).

Back

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels
The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels. Scatter plots of expression (FPKM) for cDNA libraries prepared from 100 ng and 100 pg of human brain polyA+ RNA show a high correlation, suggesting consistency across input levels. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

Back

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations
Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations. Comparisons of pairs of cDNA library replicas created from 100 ng and 100 pg of input RNA show high reproducibility across a wide range of input RNA. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

Back

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments
Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments.

Back

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis
High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis. Reads mapping to the ERCC data set from the previous experiments were pooled together and the FPKM was plotted against relative transcript abundance. The complete set of 92 ERCC transcripts was identified, with a slope of 0.934 and R2 of 0.9725. Axes are plotted on a log2 scale.

Back

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads
Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina® HiSeq® Platform with 2 x 100 bp paired end reads. Short, overlapping reads originating from different strands of the genomic DNA were distinguished from each other, enabling quantitative expression analysis and accurate genome annotation. 99% of RNA-Seq reads mapped to the correct strand.

Back

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit
Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit. Panel A. RNA-seq reads from the Human Brain Poly A+ RNA cDNA library were mapped against the human genome. The SMARTer Stranded method allowed assignment of sequencing reads to the correct gene in the case of overlapping PHC1 and M6PR transcripts. Panel B. Strand-specific coverage of the CDR1 locus. Nearly all reads are antisense to the annotated transcript, a finding independently reported elsewhere (Hansen, T. B. et al. (2011) EMBO J. 30(21):4414–4422). Panel C. Comparison of CDR1 gene counts obtained using either a strand-agnostic or strand-aware method.

Back

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis
RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Clontech) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Clontech), while retaining more noncoding reads.
634839 SMARTer® Stranded RNA-Seq Kit 96 Rxns USD $4024.00

The SMARTer Stranded RNA-Seq Kit includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform, starting from as little as 100 pg of polyA-purified or ribosomal RNA-depleted RNA. The kit consists of the SMARTer Stranded RNA-Seq Components, SeqAmp DNA Polymerase, and the llumina Indexing Primer Set (PCR primers for the amplification of indexed, paired-end Illumina-compatible sequencing libraries, which enable multiplexing of NGS library analysis).

The SMARTer Stranded RNA-Seq Kit utilizes our patented SMART (Switching Mechanism At 5' end of RNA Template) technology, coupled with PCR amplification, to generate Illumina-compatible libraries without the need for enzymatic clean-up or adapter ligations. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA.

Documents Components You May Also Like Image Data Resources

Back

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations
cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain polyA+ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit).

Back

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels
The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels. Scatter plots of expression (FPKM) for cDNA libraries prepared from 100 ng and 100 pg of human brain polyA+ RNA show a high correlation, suggesting consistency across input levels. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

Back

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations
Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations. Comparisons of pairs of cDNA library replicas created from 100 ng and 100 pg of input RNA show high reproducibility across a wide range of input RNA. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

Back

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments
Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments.

Back

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis
High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis. Reads mapping to the ERCC data set from the previous experiments were pooled together and the FPKM was plotted against relative transcript abundance. The complete set of 92 ERCC transcripts was identified, with a slope of 0.934 and R2 of 0.9725. Axes are plotted on a log2 scale.

Back

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads
Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina® HiSeq® Platform with 2 x 100 bp paired end reads. Short, overlapping reads originating from different strands of the genomic DNA were distinguished from each other, enabling quantitative expression analysis and accurate genome annotation. 99% of RNA-Seq reads mapped to the correct strand.

Back

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit
Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit. Panel A. RNA-seq reads from the Human Brain Poly A+ RNA cDNA library were mapped against the human genome. The SMARTer Stranded method allowed assignment of sequencing reads to the correct gene in the case of overlapping PHC1 and M6PR transcripts. Panel B. Strand-specific coverage of the CDR1 locus. Nearly all reads are antisense to the annotated transcript, a finding independently reported elsewhere (Hansen, T. B. et al. (2011) EMBO J. 30(21):4414–4422). Panel C. Comparison of CDR1 gene counts obtained using either a strand-agnostic or strand-aware method.

Back

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis
RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Clontech) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Clontech), while retaining more noncoding reads.
634836 SMARTer® Stranded RNA-Seq Kit 12 Rxns USD $1027.00

The SMARTer Stranded RNA-Seq Kit includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform, starting from as little as 100 pg of polyA-purified or ribosomal RNA-depleted RNA. The kit consists of the SMARTer Stranded RNA-Seq Components, SeqAmp DNA Polymerase, and the llumina Indexing Primer Set (PCR primers for the amplification of indexed, paired-end Illumina-compatible sequencing libraries, which enable multiplexing of NGS library analysis).

The SMARTer Stranded RNA-Seq Kit utilizes our patented SMART (Switching Mechanism At 5' end of RNA Template) technology, coupled with PCR amplification, to generate Illumina-compatible libraries without the need for enzymatic clean-up or adapter ligations. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data Resources

Back

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations
cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain polyA+ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit).

Back

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels
The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels. Scatter plots of expression (FPKM) for cDNA libraries prepared from 100 ng and 100 pg of human brain polyA+ RNA show a high correlation, suggesting consistency across input levels. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

Back

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations
Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations. Comparisons of pairs of cDNA library replicas created from 100 ng and 100 pg of input RNA show high reproducibility across a wide range of input RNA. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

Back

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments
Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments.

Back

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis
High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis. Reads mapping to the ERCC data set from the previous experiments were pooled together and the FPKM was plotted against relative transcript abundance. The complete set of 92 ERCC transcripts was identified, with a slope of 0.934 and R2 of 0.9725. Axes are plotted on a log2 scale.

Back

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads
Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina® HiSeq® Platform with 2 x 100 bp paired end reads. Short, overlapping reads originating from different strands of the genomic DNA were distinguished from each other, enabling quantitative expression analysis and accurate genome annotation. 99% of RNA-Seq reads mapped to the correct strand.

Back

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit
Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit. Panel A. RNA-seq reads from the Human Brain Poly A+ RNA cDNA library were mapped against the human genome. The SMARTer Stranded method allowed assignment of sequencing reads to the correct gene in the case of overlapping PHC1 and M6PR transcripts. Panel B. Strand-specific coverage of the CDR1 locus. Nearly all reads are antisense to the annotated transcript, a finding independently reported elsewhere (Hansen, T. B. et al. (2011) EMBO J. 30(21):4414–4422). Panel C. Comparison of CDR1 gene counts obtained using either a strand-agnostic or strand-aware method.

Back

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis
RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Clontech) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Clontech), while retaining more noncoding reads.
634837 SMARTer® Stranded RNA-Seq Kit 24 Rxns USD $1850.00

The SMARTer Stranded RNA-Seq Kit includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform, starting from as little as 100 pg of polyA-purified or ribosomal RNA-depleted RNA. The kit consists of the SMARTer Stranded RNA-Seq Components, SeqAmp DNA Polymerase, and the llumina Indexing Primer Set (PCR primers for the amplification of indexed, paired-end Illumina-compatible sequencing libraries, which enable multiplexing of NGS library analysis).

The SMARTer Stranded RNA-Seq Kit utilizes our patented SMART (Switching Mechanism At 5' end of RNA Template) technology, coupled with PCR amplification, to generate Illumina-compatible libraries without the need for enzymatic clean-up or adapter ligations. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data Resources

Back

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations
cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain polyA+ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit).

Back

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels
The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels. Scatter plots of expression (FPKM) for cDNA libraries prepared from 100 ng and 100 pg of human brain polyA+ RNA show a high correlation, suggesting consistency across input levels. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

Back

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations
Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations. Comparisons of pairs of cDNA library replicas created from 100 ng and 100 pg of input RNA show high reproducibility across a wide range of input RNA. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

Back

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments
Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments.

Back

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis
High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis. Reads mapping to the ERCC data set from the previous experiments were pooled together and the FPKM was plotted against relative transcript abundance. The complete set of 92 ERCC transcripts was identified, with a slope of 0.934 and R2 of 0.9725. Axes are plotted on a log2 scale.

Back

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads
Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina® HiSeq® Platform with 2 x 100 bp paired end reads. Short, overlapping reads originating from different strands of the genomic DNA were distinguished from each other, enabling quantitative expression analysis and accurate genome annotation. 99% of RNA-Seq reads mapped to the correct strand.

Back

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit
Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit. Panel A. RNA-seq reads from the Human Brain Poly A+ RNA cDNA library were mapped against the human genome. The SMARTer Stranded method allowed assignment of sequencing reads to the correct gene in the case of overlapping PHC1 and M6PR transcripts. Panel B. Strand-specific coverage of the CDR1 locus. Nearly all reads are antisense to the annotated transcript, a finding independently reported elsewhere (Hansen, T. B. et al. (2011) EMBO J. 30(21):4414–4422). Panel C. Comparison of CDR1 gene counts obtained using either a strand-agnostic or strand-aware method.

Back

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis
RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Clontech) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Clontech), while retaining more noncoding reads.
634838 SMARTer® Stranded RNA-Seq Kit 48 Rxns USD $3125.00

The SMARTer Stranded RNA-Seq Kit includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform, starting from as little as 100 pg of polyA-purified or ribosomal RNA-depleted RNA. The kit consists of the SMARTer Stranded RNA-Seq Components, SeqAmp DNA Polymerase, and the llumina Indexing Primer Set (PCR primers for the amplification of indexed, paired-end Illumina-compatible sequencing libraries, which enable multiplexing of NGS library analysis).

The SMARTer Stranded RNA-Seq Kit utilizes our patented SMART (Switching Mechanism At 5' end of RNA Template) technology, coupled with PCR amplification, to generate Illumina-compatible libraries without the need for enzymatic clean-up or adapter ligations. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data Resources

Back

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations
cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain polyA+ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit).

Back

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels
The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels. Scatter plots of expression (FPKM) for cDNA libraries prepared from 100 ng and 100 pg of human brain polyA+ RNA show a high correlation, suggesting consistency across input levels. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

Back

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations
Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations. Comparisons of pairs of cDNA library replicas created from 100 ng and 100 pg of input RNA show high reproducibility across a wide range of input RNA. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

Back

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments
Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments.

Back

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis
High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis. Reads mapping to the ERCC data set from the previous experiments were pooled together and the FPKM was plotted against relative transcript abundance. The complete set of 92 ERCC transcripts was identified, with a slope of 0.934 and R2 of 0.9725. Axes are plotted on a log2 scale.

Back

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads
Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina® HiSeq® Platform with 2 x 100 bp paired end reads. Short, overlapping reads originating from different strands of the genomic DNA were distinguished from each other, enabling quantitative expression analysis and accurate genome annotation. 99% of RNA-Seq reads mapped to the correct strand.

Back

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit
Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit. Panel A. RNA-seq reads from the Human Brain Poly A+ RNA cDNA library were mapped against the human genome. The SMARTer Stranded method allowed assignment of sequencing reads to the correct gene in the case of overlapping PHC1 and M6PR transcripts. Panel B. Strand-specific coverage of the CDR1 locus. Nearly all reads are antisense to the annotated transcript, a finding independently reported elsewhere (Hansen, T. B. et al. (2011) EMBO J. 30(21):4414–4422). Panel C. Comparison of CDR1 gene counts obtained using either a strand-agnostic or strand-aware method.

Back

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis
RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Clontech) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Clontech), while retaining more noncoding reads.
634839 SMARTer® Stranded RNA-Seq Kit 96 Rxns USD $4024.00

The SMARTer Stranded RNA-Seq Kit includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform, starting from as little as 100 pg of polyA-purified or ribosomal RNA-depleted RNA. The kit consists of the SMARTer Stranded RNA-Seq Components, SeqAmp DNA Polymerase, and the llumina Indexing Primer Set (PCR primers for the amplification of indexed, paired-end Illumina-compatible sequencing libraries, which enable multiplexing of NGS library analysis).

The SMARTer Stranded RNA-Seq Kit utilizes our patented SMART (Switching Mechanism At 5' end of RNA Template) technology, coupled with PCR amplification, to generate Illumina-compatible libraries without the need for enzymatic clean-up or adapter ligations. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations
cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain polyA+ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit).

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The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels
The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a thousand fold range of input RNA levels. Scatter plots of expression (FPKM) for cDNA libraries prepared from 100 ng and 100 pg of human brain polyA+ RNA show a high correlation, suggesting consistency across input levels. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

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Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations
Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations. Comparisons of pairs of cDNA library replicas created from 100 ng and 100 pg of input RNA show high reproducibility across a wide range of input RNA. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R).

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Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments

Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments
Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments.

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High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis
High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis. Reads mapping to the ERCC data set from the previous experiments were pooled together and the FPKM was plotted against relative transcript abundance. The complete set of 92 ERCC transcripts was identified, with a slope of 0.934 and R2 of 0.9725. Axes are plotted on a log2 scale.

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Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads
Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina® HiSeq® Platform with 2 x 100 bp paired end reads. Short, overlapping reads originating from different strands of the genomic DNA were distinguished from each other, enabling quantitative expression analysis and accurate genome annotation. 99% of RNA-Seq reads mapped to the correct strand.

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Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit
Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit. Panel A. RNA-seq reads from the Human Brain Poly A+ RNA cDNA library were mapped against the human genome. The SMARTer Stranded method allowed assignment of sequencing reads to the correct gene in the case of overlapping PHC1 and M6PR transcripts. Panel B. Strand-specific coverage of the CDR1 locus. Nearly all reads are antisense to the annotated transcript, a finding independently reported elsewhere (Hansen, T. B. et al. (2011) EMBO J. 30(21):4414–4422). Panel C. Comparison of CDR1 gene counts obtained using either a strand-agnostic or strand-aware method.

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RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis
RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Clontech) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Clontech), while retaining more noncoding reads.
634862 SMARTer® Stranded RNA-Seq Kit HT 96 Rxns USD $4109.00

The SMARTer Stranded RNA-Seq Kit HT includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform in a high-throughput manner. The kit consists of the SMARTer Stranded RNA-Seq Components, SeqAmp DNA Polymerase, and the Indexing Primer Set HT for Illumina (which contains 8 indexed forward PCR primers and 12 indexed reverse PCR primers for the high-throughput amplification of 96 uniquely-indexed RNA-seq libraries). This kit generates indexed, paired-end, Illumina-compatible sequencing libraries in a 96-well plate format, which enables high levels of multiplexing of NGS library analysis.

The SMARTer Stranded RNA-Seq Kit HT utilizes our patented SMART (Switching Mechanism At 5' end of RNA Template) technology, coupled with PCR amplification, to generate Illumina-compatible libraries without the need for enzymatic cleanup or adapter ligations. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA. The Indexing Primer Set HT for Illumina integrated into this kit makes it convenient to generate 96 uniquely-indexed libraries for Illumina sequencing.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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634873 SMARTer® Stranded Total RNA Sample Prep Kit - HI Mammalian 12 Rxns USD $1176.00

The SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian generates libraries for RNA sequencing (RNA-seq), compatible with Illumina platforms for mammalian samples. The kit is designed to work with input ranges from 100 ng to 1 μg of total RNA. This kit incorporates both RiboGone and SMART technology, and processes ribosomal RNA (rRNA) removal (5S, 5.8S, 18S, and 28S nuclear rRNA sequences), as well as some mitochondrial rRNA sequences (12S), from human, mouse, or rat full-length or sheared total RNA, followed by cDNA synthesis and Illumina library preparation. RNA-seq libraries prepared with this kit are indexed.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Reproducibility across replicates

Reproducibility across replicates
Reproducibility across replicates. RNA-seq libraries were generated from two samples of 100 ng of HURR. The scatterplot illustrates correlations between the FPKMs (Fragments Per Kilobase Of Exon Per Million Fragments Mapped) from the two libraries.

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Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation

Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation
Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation. Section A. Depletion of rRNA from total RNA samples with RiboGone technology. Section B. First-strand cDNA synthesis with SMART technology, incorporating Illumina Read Primers 1 and 2. Section C. Template switching and generation of sequencing libraries with Illumina cluster-generating sequences and indexes by PCR amplification

Back

MAQC Analysis

MAQC Analysis
MAQC Analysis. RNA-seq libraries were generated with 400 ng of HURR and HUBR. The scatter plot shows the Log2 ratio of FPKMs of HURR/HBRR graphed against the Log2 of the ratio of HURR/HBRR derived from qPCR Taqman probes.

Back

Dynamic range and linearity of RNA-seq data

Dynamic range and linearity of RNA-seq data
Dynamic range and linearity of RNA-seq data. Libraries were generated from Human Brain Reference RNA with ERCC Spike-In RNA Mix2. The above graph shows strong correlation between the Log2 of input concentrations of individual ERCC transcripts vs. the Log2 of FPKMs for those transcripts.

Back

Reproducibility across RNA quality

Reproducibility across RNA quality
Reproducibility across RNA quality. A scatterplot illustrates the correlations between the FPKMs from two libraries generated from 1 µg Mouse Liver RNA that was chemically sheared until it had a RIN of 3 or 7.

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Sequence Alignment Metrics

Sequence Alignment Metrics
Sequence Alignment Metrics. 400 ng of HURR and HBRR with ERCC Spike-In RNA were treated with this kit. Alignment data is displayed for both libraries, with the percentage of reads that mapped to rRNA, exonic regions, intronic regions, intergenic regions, and the correct strand, as defined by Picard analysis.

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High-quality libraries across varying levels of RNA quality

High-quality libraries across varying levels of RNA quality
High-quality libraries across varying levels of RNA quality. Libraries were generated from Mouse Liver RNA by chemically shearing until it had a RIN of 3 or 7. Sequencing data showed the percentage of reads that mapped to rRNA, exonic regions, intronic regions, intergenic regions, and the correct strand, as defined by Picard analysis.
634874 SMARTer® Stranded Total RNA Sample Prep Kit - HI Mammalian 24 Rxns USD $1999.00

The SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian generates libraries for RNA sequencing (RNA-seq), compatible with Illumina platforms for mammalian samples. The kit is designed to work with input ranges from 100 ng to 1 μg of total RNA. This kit incorporates both RiboGone and SMART technology, and processes ribosomal RNA (rRNA) removal (5S, 5.8S, 18S, and 28S nuclear rRNA sequences), as well as some mitochondrial rRNA sequences (12S), from human, mouse, or rat full-length or sheared total RNA, followed by cDNA synthesis and Illumina library preparation. RNA-seq libraries prepared with this kit are indexed.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Back

Reproducibility across replicates

Reproducibility across replicates
Reproducibility across replicates. RNA-seq libraries were generated from two samples of 100 ng of HURR. The scatterplot illustrates correlations between the FPKMs (Fragments Per Kilobase Of Exon Per Million Fragments Mapped) from the two libraries.

Back

Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation

Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation
Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation. Section A. Depletion of rRNA from total RNA samples with RiboGone technology. Section B. First-strand cDNA synthesis with SMART technology, incorporating Illumina Read Primers 1 and 2. Section C. Template switching and generation of sequencing libraries with Illumina cluster-generating sequences and indexes by PCR amplification

Back

MAQC Analysis

MAQC Analysis
MAQC Analysis. RNA-seq libraries were generated with 400 ng of HURR and HUBR. The scatter plot shows the Log2 ratio of FPKMs of HURR/HBRR graphed against the Log2 of the ratio of HURR/HBRR derived from qPCR Taqman probes.

Back

Dynamic range and linearity of RNA-seq data

Dynamic range and linearity of RNA-seq data
Dynamic range and linearity of RNA-seq data. Libraries were generated from Human Brain Reference RNA with ERCC Spike-In RNA Mix2. The above graph shows strong correlation between the Log2 of input concentrations of individual ERCC transcripts vs. the Log2 of FPKMs for those transcripts.

Back

Reproducibility across RNA quality

Reproducibility across RNA quality
Reproducibility across RNA quality. A scatterplot illustrates the correlations between the FPKMs from two libraries generated from 1 µg Mouse Liver RNA that was chemically sheared until it had a RIN of 3 or 7.

Back

Sequence Alignment Metrics

Sequence Alignment Metrics
Sequence Alignment Metrics. 400 ng of HURR and HBRR with ERCC Spike-In RNA were treated with this kit. Alignment data is displayed for both libraries, with the percentage of reads that mapped to rRNA, exonic regions, intronic regions, intergenic regions, and the correct strand, as defined by Picard analysis.

Back

High-quality libraries across varying levels of RNA quality

High-quality libraries across varying levels of RNA quality
High-quality libraries across varying levels of RNA quality. Libraries were generated from Mouse Liver RNA by chemically shearing until it had a RIN of 3 or 7. Sequencing data showed the percentage of reads that mapped to rRNA, exonic regions, intronic regions, intergenic regions, and the correct strand, as defined by Picard analysis.
634875 SMARTer® Stranded Total RNA Sample Prep Kit - HI Mammalian 48 Rxns USD $3397.00

The SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian generates libraries for RNA sequencing (RNA-seq), compatible with Illumina platforms for mammalian samples. The kit is designed to work with input ranges from 100 ng to 1 μg of total RNA. This kit incorporates both RiboGone and SMART technology, and processes ribosomal RNA (rRNA) removal (5S, 5.8S, 18S, and 28S nuclear rRNA sequences), as well as some mitochondrial rRNA sequences (12S), from human, mouse, or rat full-length or sheared total RNA, followed by cDNA synthesis and Illumina library preparation. RNA-seq libraries prepared with this kit are indexed.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Back

Reproducibility across replicates

Reproducibility across replicates
Reproducibility across replicates. RNA-seq libraries were generated from two samples of 100 ng of HURR. The scatterplot illustrates correlations between the FPKMs (Fragments Per Kilobase Of Exon Per Million Fragments Mapped) from the two libraries.

Back

Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation

Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation
Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation. Section A. Depletion of rRNA from total RNA samples with RiboGone technology. Section B. First-strand cDNA synthesis with SMART technology, incorporating Illumina Read Primers 1 and 2. Section C. Template switching and generation of sequencing libraries with Illumina cluster-generating sequences and indexes by PCR amplification

Back

MAQC Analysis

MAQC Analysis
MAQC Analysis. RNA-seq libraries were generated with 400 ng of HURR and HUBR. The scatter plot shows the Log2 ratio of FPKMs of HURR/HBRR graphed against the Log2 of the ratio of HURR/HBRR derived from qPCR Taqman probes.

Back

Dynamic range and linearity of RNA-seq data

Dynamic range and linearity of RNA-seq data
Dynamic range and linearity of RNA-seq data. Libraries were generated from Human Brain Reference RNA with ERCC Spike-In RNA Mix2. The above graph shows strong correlation between the Log2 of input concentrations of individual ERCC transcripts vs. the Log2 of FPKMs for those transcripts.

Back

Reproducibility across RNA quality

Reproducibility across RNA quality
Reproducibility across RNA quality. A scatterplot illustrates the correlations between the FPKMs from two libraries generated from 1 µg Mouse Liver RNA that was chemically sheared until it had a RIN of 3 or 7.

Back

Sequence Alignment Metrics

Sequence Alignment Metrics
Sequence Alignment Metrics. 400 ng of HURR and HBRR with ERCC Spike-In RNA were treated with this kit. Alignment data is displayed for both libraries, with the percentage of reads that mapped to rRNA, exonic regions, intronic regions, intergenic regions, and the correct strand, as defined by Picard analysis.

Back

High-quality libraries across varying levels of RNA quality

High-quality libraries across varying levels of RNA quality
High-quality libraries across varying levels of RNA quality. Libraries were generated from Mouse Liver RNA by chemically shearing until it had a RIN of 3 or 7. Sequencing data showed the percentage of reads that mapped to rRNA, exonic regions, intronic regions, intergenic regions, and the correct strand, as defined by Picard analysis.
634876 SMARTer® Stranded Total RNA Sample Prep Kit - HI Mammalian 96 Rxns USD $4977.00

The SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian generates libraries for RNA sequencing (RNA-seq), compatible with Illumina platforms for mammalian samples. The kit is designed to work with input ranges from 100 ng to 1 μg of total RNA. This kit incorporates both RiboGone and SMART technology, and processes ribosomal RNA (rRNA) removal (5S, 5.8S, 18S, and 28S nuclear rRNA sequences), as well as some mitochondrial rRNA sequences (12S), from human, mouse, or rat full-length or sheared total RNA, followed by cDNA synthesis and Illumina library preparation. RNA-seq libraries prepared with this kit are indexed.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Back

Reproducibility across replicates

Reproducibility across replicates
Reproducibility across replicates. RNA-seq libraries were generated from two samples of 100 ng of HURR. The scatterplot illustrates correlations between the FPKMs (Fragments Per Kilobase Of Exon Per Million Fragments Mapped) from the two libraries.

Back

Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation

Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation
Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation. Section A. Depletion of rRNA from total RNA samples with RiboGone technology. Section B. First-strand cDNA synthesis with SMART technology, incorporating Illumina Read Primers 1 and 2. Section C. Template switching and generation of sequencing libraries with Illumina cluster-generating sequences and indexes by PCR amplification

Back

MAQC Analysis

MAQC Analysis
MAQC Analysis. RNA-seq libraries were generated with 400 ng of HURR and HUBR. The scatter plot shows the Log2 ratio of FPKMs of HURR/HBRR graphed against the Log2 of the ratio of HURR/HBRR derived from qPCR Taqman probes.

Back

Dynamic range and linearity of RNA-seq data

Dynamic range and linearity of RNA-seq data
Dynamic range and linearity of RNA-seq data. Libraries were generated from Human Brain Reference RNA with ERCC Spike-In RNA Mix2. The above graph shows strong correlation between the Log2 of input concentrations of individual ERCC transcripts vs. the Log2 of FPKMs for those transcripts.

Back

Reproducibility across RNA quality

Reproducibility across RNA quality
Reproducibility across RNA quality. A scatterplot illustrates the correlations between the FPKMs from two libraries generated from 1 µg Mouse Liver RNA that was chemically sheared until it had a RIN of 3 or 7.

Back

Sequence Alignment Metrics

Sequence Alignment Metrics
Sequence Alignment Metrics. 400 ng of HURR and HBRR with ERCC Spike-In RNA were treated with this kit. Alignment data is displayed for both libraries, with the percentage of reads that mapped to rRNA, exonic regions, intronic regions, intergenic regions, and the correct strand, as defined by Picard analysis.

Back

High-quality libraries across varying levels of RNA quality

High-quality libraries across varying levels of RNA quality
High-quality libraries across varying levels of RNA quality. Libraries were generated from Mouse Liver RNA by chemically shearing until it had a RIN of 3 or 7. Sequencing data showed the percentage of reads that mapped to rRNA, exonic regions, intronic regions, intergenic regions, and the correct strand, as defined by Picard analysis.
634877 SMARTer® Stranded Total RNA Sample Prep Kit - HI Mammalian 192 Rxns USD $8900.00

The SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian generates libraries for RNA sequencing (RNA-seq), compatible with Illumina platforms for mammalian samples. The kit is designed to work with input ranges from 100 ng to 1 μg of total RNA. This kit incorporates both RiboGone and SMART technology, and processes ribosomal RNA (rRNA) removal (5S, 5.8S, 18S, and 28S nuclear rRNA sequences), as well as some mitochondrial rRNA sequences (12S), from human, mouse, or rat full-length or sheared total RNA, followed by cDNA synthesis and Illumina library preparation. RNA-seq libraries prepared with this kit are indexed.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data Resources

Back

Reproducibility across replicates

Reproducibility across replicates
Reproducibility across replicates. RNA-seq libraries were generated from two samples of 100 ng of HURR. The scatterplot illustrates correlations between the FPKMs (Fragments Per Kilobase Of Exon Per Million Fragments Mapped) from the two libraries.

Back

Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation

Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation
Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation. Section A. Depletion of rRNA from total RNA samples with RiboGone technology. Section B. First-strand cDNA synthesis with SMART technology, incorporating Illumina Read Primers 1 and 2. Section C. Template switching and generation of sequencing libraries with Illumina cluster-generating sequences and indexes by PCR amplification

Back

MAQC Analysis

MAQC Analysis
MAQC Analysis. RNA-seq libraries were generated with 400 ng of HURR and HUBR. The scatter plot shows the Log2 ratio of FPKMs of HURR/HBRR graphed against the Log2 of the ratio of HURR/HBRR derived from qPCR Taqman probes.

Back

Dynamic range and linearity of RNA-seq data

Dynamic range and linearity of RNA-seq data
Dynamic range and linearity of RNA-seq data. Libraries were generated from Human Brain Reference RNA with ERCC Spike-In RNA Mix2. The above graph shows strong correlation between the Log2 of input concentrations of individual ERCC transcripts vs. the Log2 of FPKMs for those transcripts.

Back

Reproducibility across RNA quality

Reproducibility across RNA quality
Reproducibility across RNA quality. A scatterplot illustrates the correlations between the FPKMs from two libraries generated from 1 µg Mouse Liver RNA that was chemically sheared until it had a RIN of 3 or 7.

Back

Sequence Alignment Metrics

Sequence Alignment Metrics
Sequence Alignment Metrics. 400 ng of HURR and HBRR with ERCC Spike-In RNA were treated with this kit. Alignment data is displayed for both libraries, with the percentage of reads that mapped to rRNA, exonic regions, intronic regions, intergenic regions, and the correct strand, as defined by Picard analysis.

Back

High-quality libraries across varying levels of RNA quality

High-quality libraries across varying levels of RNA quality
High-quality libraries across varying levels of RNA quality. Libraries were generated from Mouse Liver RNA by chemically shearing until it had a RIN of 3 or 7. Sequencing data showed the percentage of reads that mapped to rRNA, exonic regions, intronic regions, intergenic regions, and the correct strand, as defined by Picard analysis.
634878 SMARTer® Stranded Total RNA Sample Prep Kit - HI Mammalian 480 Rxns USD $18254.00

The SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian generates libraries for RNA sequencing (RNA-seq), compatible with Illumina platforms for mammalian samples. The kit is designed to work with input ranges from 100 ng to 1 μg of total RNA. This kit incorporates both RiboGone and SMART technology, and processes ribosomal RNA (rRNA) removal (5S, 5.8S, 18S, and 28S nuclear rRNA sequences), as well as some mitochondrial rRNA sequences (12S), from human, mouse, or rat full-length or sheared total RNA, followed by cDNA synthesis and Illumina library preparation. RNA-seq libraries prepared with this kit are indexed.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data Resources

Back

Reproducibility across replicates

Reproducibility across replicates
Reproducibility across replicates. RNA-seq libraries were generated from two samples of 100 ng of HURR. The scatterplot illustrates correlations between the FPKMs (Fragments Per Kilobase Of Exon Per Million Fragments Mapped) from the two libraries.

Back

Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation

Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation
Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation. Section A. Depletion of rRNA from total RNA samples with RiboGone technology. Section B. First-strand cDNA synthesis with SMART technology, incorporating Illumina Read Primers 1 and 2. Section C. Template switching and generation of sequencing libraries with Illumina cluster-generating sequences and indexes by PCR amplification

Back

MAQC Analysis

MAQC Analysis
MAQC Analysis. RNA-seq libraries were generated with 400 ng of HURR and HUBR. The scatter plot shows the Log2 ratio of FPKMs of HURR/HBRR graphed against the Log2 of the ratio of HURR/HBRR derived from qPCR Taqman probes.

Back

Dynamic range and linearity of RNA-seq data

Dynamic range and linearity of RNA-seq data
Dynamic range and linearity of RNA-seq data. Libraries were generated from Human Brain Reference RNA with ERCC Spike-In RNA Mix2. The above graph shows strong correlation between the Log2 of input concentrations of individual ERCC transcripts vs. the Log2 of FPKMs for those transcripts.

Back

Reproducibility across RNA quality

Reproducibility across RNA quality
Reproducibility across RNA quality. A scatterplot illustrates the correlations between the FPKMs from two libraries generated from 1 µg Mouse Liver RNA that was chemically sheared until it had a RIN of 3 or 7.

Back

Sequence Alignment Metrics

Sequence Alignment Metrics
Sequence Alignment Metrics. 400 ng of HURR and HBRR with ERCC Spike-In RNA were treated with this kit. Alignment data is displayed for both libraries, with the percentage of reads that mapped to rRNA, exonic regions, intronic regions, intergenic regions, and the correct strand, as defined by Picard analysis.

Back

High-quality libraries across varying levels of RNA quality

High-quality libraries across varying levels of RNA quality
High-quality libraries across varying levels of RNA quality. Libraries were generated from Mouse Liver RNA by chemically shearing until it had a RIN of 3 or 7. Sequencing data showed the percentage of reads that mapped to rRNA, exonic regions, intronic regions, intergenic regions, and the correct strand, as defined by Picard analysis.
634861 SMARTer® Stranded Total RNA Sample Prep Kit - Low Input Mammalian 24 Rxns USD $2895.00

License Statement

ID Number  
246 This product is protected by U.S. Patent 9,428,794, and additional pending US and foreign patents. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.

The SMARTer Stranded Total RNA Sample Prep Kit - Low Input Mammalian provides a complete solution for sensitive and streamlined total RNA analysis. The kit is designed to work with a wide range of input amounts (10–100 ng) of total RNA of any quality. A variety of human, rat, and mouse samples can be used, including total RNA from FFPE material, tissue samples, etc. The kit provides whole transcriptome analysis with accurate measurement of strand orientation, unbiased coverage, high transcript mapping, and gene counts.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data Resources

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Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit
Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit. Panel A. RNA-seq reads from the Human Brain Poly A+ RNA cDNA library were mapped against the human genome. The SMARTer Stranded method allowed assignment of sequencing reads to the correct gene in the case of overlapping PHC1 and M6PR transcripts. Panel B. Strand-specific coverage of the CDR1 locus. Nearly all reads are antisense to the annotated transcript, a finding independently reported elsewhere (Hansen, T. B. et al. (2011) EMBO J. 30(21):4414–4422). Panel C. Comparison of CDR1 gene counts obtained using either a strand-agnostic or strand-aware method.

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RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis
RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Clontech) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Clontech), while retaining more noncoding reads.

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Capturem Trypsin for a rapid, efficient mass spectometry workflow at room temperature.

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Capturem Trypsin provides rapid, efficient, and complete digestion of protein samples, allowing an uninterrupted mass spectometry workflow at room temperature for downstream protein analysis. This product utilizes our novel Capturem technology in a spin column format with membrane-immobilized trypsin. Capturem Trypsin Columns may be used to completely digest protein samples in less than a minute with digestion efficiencies (protein coverage) comparable to or better than those obtained using in-solution trypsin digestion.

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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