NucleoSnap DNA Plasma
The NucleoSnap DNA Plasma kit is designed for isolating circulating cell-free DNA (cfDNA) from plasma. NucleoSnap columns are equipped with a predetermined breaking point and can be divided into a funnel component and a mini spin column by simply snapping off the funnel component. These columns allow for an ultra-fast procedure, with final elution from a mini spin column using a microcentrifuge after vacuum processing. NucleoSnap DNA Plasma columns are optimized for DNA isolation, with no need for carrier RNA.
The NucleoSnap DNA Plasma kit is designed for isolating circulating cell-free DNA (cfDNA) from plasma. NucleoSnap columns are equipped with a predetermined breaking point and can be divided into a funnel component and a mini spin column by simply snapping off the funnel component. These columns allow for an ultra-fast procedure, with final elution from a mini spin column using a microcentrifuge after vacuum processing. NucleoSnap DNA Plasma columns are optimized for DNA isolation, with no need for carrier RNA.
Figure 1. The NucleoSnap (snap-off) column splits into mini spin column and funnel components.
Overview
- New column design for vacuum processing of large sample volumes
- Single piece of plastic (mini spin column attached to a funnel component) can be separated for final elution
- Ultra-fast procedure allows elution from a mini spin column using a microcentrifuge
- Column is optimized for DNA isolation, with no need for carrier RNA
More Information
| Technology | Silica membrane technology |
| Processing | Vacuum processing, manual |
| Format | Snap-off column |
| Starting material | EDTA, Cell-Free DNA BCT (Streck) |
| Sample input | 1–10 ml plasma (1–5 ml human EDTA/Streck plasma, protocol for 10 ml available) |
| Fragment size | >50 bp |
| Typical yield | Range of 0.1 to 100 ng DNA per ml plasma |
| Elution volume | 20–100 μl |
| Preparation time | 45 min/6 preps |
| Carrier | Not needed |
| Buffer preparation | All buffers and components are ready to use |
Applications
- Isolation of circulating cell-free DNA (cfDNA) from plasma
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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Using novel chemistry and a unique snap-off column design, NucleoSnap Plasmid Midi provides rapid purification of up to 700 µg of transfection-grade plasmid. The snap-off columns allow processing of lysate from large 50-ml culture volumes, and multiple samples can be rapidly processed by vacuum using our NucleoVac 24 Vacuum Manifold.
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