NucleoSnap cfDNA
The NucleoSnap cfDNA kit is designed for isolating circulating cell-free DNA (cfDNA) from plasma. NucleoSnap columns are equipped with a predetermined breaking point and can be divided into a funnel component and a mini spin column by simply snapping off the funnel component. These columns allow for an ultra-fast procedure, with final elution from a mini spin column using a microcentrifuge after vacuum processing. NucleoSnap cfDNA columns are optimized for DNA isolation, with no need for carrier RNA.
The NucleoSnap cfDNA kit is designed for isolating circulating cell-free DNA (cfDNA) from plasma. NucleoSnap columns are equipped with a predetermined breaking point and can be divided into a funnel component and a mini spin column by simply snapping off the funnel component. These columns allow for an ultra-fast procedure, with final elution from a mini spin column using a microcentrifuge after vacuum processing. NucleoSnap DNA Plasma columns are optimized for DNA isolation, with no need for carrier RNA.
Figure 1. The NucleoSnap (snap-off) column splits into mini spin column and funnel components.
Overview
- New column design for vacuum processing of large sample volumes
- Single piece of plastic (mini spin column attached to a funnel component) can be separated for final elution
- Ultra-fast procedure allows elution from a mini spin column using a microcentrifuge
- Column is optimized for DNA isolation, with no need for carrier RNA
More Information
| Technology | Silica-membrane technology |
| Processing | Vacuum processing, manual |
| Format | Snap-off column |
| Starting material | EDTA, Cell-Free DNA BCT (Streck) |
| Sample input | 1–10 ml plasma (1–5 ml human EDTA/Streck plasma, protocol for 10 ml available) |
| Fragment size | >50 bp |
| Typical yield | Range of 0.1 to 100 ng DNA per ml plasma |
| Elution volume | 20–100 μl |
| Preparation time | 45 min/6 preps |
| Carrier | Not needed |
| Buffer preparation | All buffers and components are ready to use |
Applications
- Isolation of circulating cell-free DNA (cfDNA) from plasma
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Isolate up to 700 µg of transfection-grade plasmid DNA in only 35 minutes
Using novel chemistry and a unique snap-off column design, NucleoSnap Plasmid Midi provides rapid purification of up to 700 µg of transfection-grade plasmid. The snap-off columns allow processing of lysate from large 50-ml culture volumes, and multiple samples can be rapidly processed by vacuum using our NucleoVac 24 Vacuum Manifold.
Learn more Other plasmid kits
Cell-free DNA-seq library prep in under 2 hours
ThruPLEX Plasma-Seq Kit
- Efficient library preparation from as little as 1 ng of cell-free DNA
- Excellent complexity and GC representation
- Three-step, single-tube protocol can be completed in less than 2 hours
- UDIs or dual index options for multiplexing up to 384 samples
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2021 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.
