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Nucleic acid purification

Isolation of RNA from soil—NucleoBond RNA Soil and NucleoBond RNA Soil Mini

Efficient isolation of up to 600 µg of high-quality RNA from any kind of soil or sediment sample.

The NucleoBond RNA Soil and NucleoBond RNA Soil Mini kits are designed for the purification of RNA from soil and sediment samples. In combination with the DNA Set for NucleoBond RNA Soil (or DNA Set for NucleoBond RNA Soil Mini), RNA and DNA can be isolated from the same soil sample. Purified nucleic acids are suitable for metagenomic studies, for example, when the genome and transcriptome are analyzed from the same sample. NucleoBond RNA Soil Mini is a downscaled version of the NucleoBond RNA Soil kit.

Most soils contain relatively low amounts of RNA, compared to DNA. Therefore, these kits were designed with proven NucleoBond technology to enable variable sample inputs and yield pure nucleic acids. Sample lysis using our MN Bead Tubes Type A in the presence of the optimized Lysis Buffer E1 and phenol:chloroform:isoamyl alcohol ensures efficient sample disruption. The optional Buffer OPT enables efficient RNA and DNA purification from mineral and organic soil samples.

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Product

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Price

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740140.20

NucleoBond® RNA Soil

20 Preps

USD $458.00

The NucleoBond RNA Soil kit employs anion exchange chromatography and silica spin purification for isolation of RNA from soil and sediment samples, and can be used in combination with the DNA Set for NucleoBond RNA Soil to obtain both DNA and RNA from the same sample. Nucleic acids purified with these kits are suitable for metagenomic studies, including genomic and transcriptomic analyses. Most soils contain relatively low amounts of RNA compared to DNA. Therefore, this kit was designed to accommodate high sample inputs in order to yield 1–10 µg of RNA. Sample lysis using MN Bead Tubes Type A in the presence of the optimized Lysis Buffer E1 and phenol:chloroform:isoamyl alcohol ensures efficient sample disruption. The optional Buffer OPT enables efficient RNA and DNA purification from mineralic and organic soil samples. NucleoBond RNA Soil enables manual processing of 12 samples in approximately 1 hour. The kit includes sufficient quantities of reagents and materials for processing of 20 samples.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

740141.20

DNA Set for NucleoBond® RNA Soil

20 Preps

USD $91.00

DNA Set for NucleoBond RNA Soil is a 20-prep elution set designed to be used in conjunction with NucleoBond RNA Soil (Cat. # 740140.20) for parallel isolation of DNA and RNA from the same soil sample. Nucleic acids purified with these kits are suitable for metagenomic studies, including genomic and transcriptomic analyses. DNA Set for NucleoBond RNA Soil typically yields 5–50 µg of pure DNA from 1–2 g of soil. The kit includes sufficient quantities of reagents and materials for processing of 20 samples.

Notice to purchaser

Required Products

Cat. #	Product	Size	Price	License	Quantity	Details
740140.20	NucleoBond® RNA Soil	20 Preps	USD $458.00
The NucleoBond RNA Soil kit employs anion exchange chromatography and silica spin purification for isolation of RNA from soil and sediment samples, and can be used in combination with the DNA Set for NucleoBond RNA Soil to obtain both DNA and RNA from the same sample. Nucleic acids purified with these kits are suitable for metagenomic studies, including genomic and transcriptomic analyses. Most soils contain relatively low amounts of RNA compared to DNA. Therefore, this kit was designed to accommodate high sample inputs in order to yield 1–10 µg of RNA. Sample lysis using MN Bead Tubes Type A in the presence of the optimized Lysis Buffer E1 and phenol:chloroform:isoamyl alcohol ensures efficient sample disruption. The optional Buffer OPT enables efficient RNA and DNA purification from mineralic and organic soil samples. NucleoBond RNA Soil enables manual processing of 12 samples in approximately 1 hour. The kit includes sufficient quantities of reagents and materials for processing of 20 samples. Documents You May Also Like Image Data Back 740140.2: NucleoBond RNA Soil Back NucleoBond RNA Soil procedure NucleoBond RNA Soil procedure. Back High RNA yields with NucleoBond RNA Soil High RNA yields with NucleoBond RNA Soil. Various soil samples (clay, shore soil, and forest soil) were purified in duplicate with NucleoBond RNA Soil using the standard kit protocol. For comparison, the samples were processed in parallel using competitor kit "Q" and the corresponding kit protocol. Each RNA sample was eluted in a volume of 100 µl and yields were determined photometrically (left panel). In addition, 15 µl of each eluate was analyzed by gel electrophoresis (1% TAE; right panel). For each sample type, NucleoBond RNA Soil provided a higher yield compared to the competitor kit. Back Amplifiable RNA for perfect results with NucleoBond RNA Soil Amplifiable RNA for perfect results with NucleoBond RNA Soil. Various soil samples (clay, shore soil, and forest soil) were purified in duplicate using NucleoBond RNA Soil and competitor kit "Q" by following the standard protocol for each kit. RT-PCR was then performed using 40 µl of eluate from each sample (amplicon size = 466 bp). Samples prepared using NucleoBond RNA Soil demonstrated lower Cp values relative to corresponding samples prepared using kit "Q". Back NucleoBond RNA Soil enables efficient parallel DNA isolation NucleoBond RNA Soil enables efficient parallel DNA isolation. Various soil samples (clay, shore soil, and forest soil) were purified in duplicate using NucleoBond RNA Soil in combination with the DNA Set for NucleoBond RNA Soil following the standard kit protocol. For comparison, the samples were processed in parallel using competitor kit "Q" and the corresponding protocol. DNA samples were eluted in a volume of 100 µl and yields were determined photometrically (left panel). In addition, 15 µl of each eluate was analyzed by gel electrophoresis (1% TAE; right panel). For each sample type, NucleoBond RNA Soil combined with the DNA Set for NucleoBond RNA Soil provided a higher yield of DNA compared to the competitor kit.

Overview

Anion exchange technology to optimize RNA yield and purity
Ideal for metagenomic studies
Combination of mechanical homogenization and chemical lysis allows processing of large sample amounts
Parallel preparation of RNA and DNA in one hour

More Information

	NucleoBond RNA Soil	NucleoBond RNA Soil Mini
Technology	Anion-exchange chromatography
Format	Gravity flow columns combined with MN Bead Tubes Type A
Starting material	<2 g of soil	0.25–0.5 g soil
Fragment size	≥100 nt
Typical yield	1–10 µg	0.25–2.5 µg
A₂₆₀/A₂₈₀	1.7–2.1	1.5–2.0
A₂₆₀/A₂₃₀	1.8–2.5	—
Typical RIN (RNA integrity number)	>8.5	>7.0
Elution volume	100 µl	50–100 µl
Preparation time	60 min/6 preps	60 min/12 preps
Binding capacity	600 µg	30 µg

Applications

RNA isolation from soil for metagenomic analysis
Purified RNA is suitable for:

Real-time RT-PCR
Northern blotting
Primer extension
Array technology
RNase protection assays

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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Isolation of RNA from soil—NucleoBond RNA Soil and NucleoBond RNA Soil Mini

Notice to purchaser

740140.2: NucleoBond RNA Soil

NucleoBond RNA Soil procedure

High RNA yields with NucleoBond RNA Soil

Amplifiable RNA for perfect results with NucleoBond RNA Soil

NucleoBond RNA Soil enables efficient parallel DNA isolation

Notice to purchaser

NucleoBond RNA Soil procedure

High RNA yields with NucleoBond RNA Soil

Amplifiable RNA for perfect results with NucleoBond RNA Soil

NucleoBond RNA Soil enables efficient parallel DNA isolation

740140.2: NucleoBond RNA Soil

NucleoBond RNA Soil procedure

High RNA yields with NucleoBond RNA Soil

Amplifiable RNA for perfect results with NucleoBond RNA Soil

NucleoBond RNA Soil enables efficient parallel DNA isolation

Overview

More Information

Applications

Additional product information