Isolation of RNA from soil—NucleoBond RNA Soil and NucleoBond RNA Soil Mini

DNA Set for NucleoBond RNA Soil is a 20-prep elution set designed to be used in conjunction with NucleoBond RNA Soil (Cat. # 740140.20) for parallel isolation of DNA and RNA from the same soil sample. Nucleic acids purified with these kits are suitable for metagenomic studies, including genomic and transcriptomic analyses. DNA Set for NucleoBond RNA Soil typically yields 5–50 µg of pure DNA from 1–2 g of soil. The kit includes sufficient quantities of reagents and materials for processing of 20 samples.

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NucleoBond RNA Soil procedure

NucleoBond RNA Soil procedure.

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High RNA yields with NucleoBond RNA Soil

High RNA yields with NucleoBond RNA Soil. Various soil samples (clay, shore soil, and forest soil) were purified in duplicate with NucleoBond RNA Soil using the standard kit protocol. For comparison, the samples were processed in parallel using competitor kit "Q" and the corresponding kit protocol. Each RNA sample was eluted in a volume of 100 µl and yields were determined photometrically (left panel). In addition, 15 µl of each eluate was analyzed by gel electrophoresis (1% TAE; right panel). For each sample type, NucleoBond RNA Soil provided a higher yield compared to the competitor kit.

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Amplifiable RNA for perfect results with NucleoBond RNA Soil

Amplifiable RNA for perfect results with NucleoBond RNA Soil. Various soil samples (clay, shore soil, and forest soil) were purified in duplicate using NucleoBond RNA Soil and competitor kit "Q" by following the standard protocol for each kit. RT-PCR was then performed using 40 µl of eluate from each sample (amplicon size = 466 bp). Samples prepared using NucleoBond RNA Soil demonstrated lower Cp values relative to corresponding samples prepared using kit "Q".

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NucleoBond RNA Soil enables efficient parallel DNA isolation

NucleoBond RNA Soil enables efficient parallel DNA isolation. Various soil samples (clay, shore soil, and forest soil) were purified in duplicate using NucleoBond RNA Soil in combination with the DNA Set for NucleoBond RNA Soil following the standard kit protocol. For comparison, the samples were processed in parallel using competitor kit "Q" and the corresponding protocol. DNA samples were eluted in a volume of 100 µl and yields were determined photometrically (left panel). In addition, 15 µl of each eluate was analyzed by gel electrophoresis (1% TAE; right panel). For each sample type, NucleoBond RNA Soil combined with the DNA Set for NucleoBond RNA Soil provided a higher yield of DNA compared to the competitor kit.

Required Products

Cat. #	Product	Size	Price	License	Quantity	Details
740140.20	NucleoBond® RNA Soil	20 Preps	$357.00
The NucleoBond RNA Soil kit employs anion exchange chromatography and silica spin purification for isolation of RNA from soil and sediment samples, and can be used in combination with the DNA Set for NucleoBond RNA Soil to obtain both DNA and RNA from the same sample. Nucleic acids purified with these kits are suitable for metagenomic studies, including genomic and transcriptomic analyses. Most soils contain relatively low amounts of RNA compared to DNA. Therefore, this kit was designed to accomodate high sample inputs in order to yield 1–10 µg of RNA. Sample lysis using NucleoSpin Bead Tubes Type A in the presence of the optimized Lysis Buffer E1 and phenol:chloroform:isoamyl alcohol ensures efficient sample disruption. The optional Buffer OPT enables efficient RNA and DNA purification from mineralic and organic soil samples. NucleoBond RNA Soil enables manual processing of 12 samples in approximately 1 hour. The kit includes sufficient quantities of reagents and materials for processing of 20 samples. Documents You May Also Like Image Data Back NucleoBond RNA Soil procedure NucleoBond RNA Soil procedure. Back High RNA yields with NucleoBond RNA Soil High RNA yields with NucleoBond RNA Soil. Various soil samples (clay, shore soil, and forest soil) were purified in duplicate with NucleoBond RNA Soil using the standard kit protocol. For comparison, the samples were processed in parallel using competitor kit "Q" and the corresponding kit protocol. Each RNA sample was eluted in a volume of 100 µl and yields were determined photometrically (left panel). In addition, 15 µl of each eluate was analyzed by gel electrophoresis (1% TAE; right panel). For each sample type, NucleoBond RNA Soil provided a higher yield compared to the competitor kit. Back Amplifiable RNA for perfect results with NucleoBond RNA Soil Amplifiable RNA for perfect results with NucleoBond RNA Soil. Various soil samples (clay, shore soil, and forest soil) were purified in duplicate using NucleoBond RNA Soil and competitor kit "Q" by following the standard protocol for each kit. RT-PCR was then performed using 40 µl of eluate from each sample (amplicon size = 466 bp). Samples prepared using NucleoBond RNA Soil demonstrated lower Cp values relative to corresponding samples prepared using kit "Q". Back NucleoBond RNA Soil enables efficient parallel DNA isolation NucleoBond RNA Soil enables efficient parallel DNA isolation. Various soil samples (clay, shore soil, and forest soil) were purified in duplicate using NucleoBond RNA Soil in combination with the DNA Set for NucleoBond RNA Soil following the standard kit protocol. For comparison, the samples were processed in parallel using competitor kit "Q" and the corresponding protocol. DNA samples were eluted in a volume of 100 µl and yields were determined photometrically (left panel). In addition, 15 µl of each eluate was analyzed by gel electrophoresis (1% TAE; right panel). For each sample type, NucleoBond RNA Soil combined with the DNA Set for NucleoBond RNA Soil provided a higher yield of DNA compared to the competitor kit.

DNA Set for NucleoBond RNA Soil Mini is a 10-prep elution set designed to be used in conjunction with NucleoBond RNA Soil Mini (Cat. # 740142.10) for parallel isolation of DNA and RNA from the same soil sample. Nucleic acids purified with these kits are suitable for metagenomic studies, including genomic and transcriptomic analyses. DNA Set for NucleoBond RNA Soil Mini typically yields 1.25–12.5 µg of pure DNA from 0.25–0.5 g of soil. The kit includes sufficient quantities of reagents and materials for processing of 10 samples.

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NucleoBond RNA Soil Mini enables efficient parallel DNA isolation when paired with the DNA Set for NucleoBond RNA Soil Mini

NucleoBond RNA Soil Mini enables efficient parallel DNA isolation when paired with the DNA Set for NucleoBond RNA Soil Mini. Two different soil samples were purified in duplicate according to the standard protocols for NucleoBond RNA Soil Mini and NucleoBond RNA Soil, respectively. DNA was eluted in 100 µl and measured photometrically. 15 µl of each eluate was analyzed on a 1% TAE gel. DNA yields obtained using NucleoBond RNA Soil Mini in tandem with the DNA Set for NucleoBond RNA Soil Mini were approximately 3–4 times lower in comparison to DNA yields obtained with NucleoBond RNA Soil and the DNA Set for NucleoBond RNA Soil.

Required Products

Cat. #	Product	Size	Price	License	Quantity	Details
740142.10	NucleoBond® RNA Soil Mini	10 Preps	$141.00
The NucleoBond RNA Soil Mini kit employs anion exchange chromatography and silica spin purification for isolation of RNA from soil and sediment samples, and can be used in combination with the DNA Set for NucleoBond RNA Soil Mini to obtain both DNA and RNA from the same sample. Nucleic acids purified with these kits are suitable for metagenomic studies. including genomic and transcriptomic analyses. Most soils contain relatively low amounts of RNA compared to DNA. Therefore, this kit was designed to accommodate sample inputs of 0.25–0.5 g in order to yield 0.25–2.5 µg of RNA. Sample lysis using NucleoSpin Bead Tubes Type A in the presence of the optimized Lysis Buffer E1 and phenol:chloroform:isoamyl alcohol ensures efficient sample disruption. The optional Buffer OPT enables efficient RNA and DNA purification from mineral and organic soil samples. NucleoBond RNA Soil Mini enables manual processing of 12 samples in approximately 1 hour. The kit includes sufficient quantities of reagents and materials for processing of 10 samples. Documents You May Also Like Image Data Back NucleoBond RNA Soil Mini enables efficient parallel DNA isolation when paired with the DNA Set for NucleoBond RNA Soil Mini NucleoBond RNA Soil Mini enables efficient parallel DNA isolation when paired with the DNA Set for NucleoBond RNA Soil Mini. Two different soil samples were purified in duplicate according to the standard protocols for NucleoBond RNA Soil Mini and NucleoBond RNA Soil, respectively. DNA was eluted in 100 µl and measured photometrically. 15 µl of each eluate was analyzed on a 1% TAE gel. DNA yields obtained using NucleoBond RNA Soil Mini in tandem with the DNA Set for NucleoBond RNA Soil Mini were approximately 3–4 times lower in comparison to DNA yields obtained with NucleoBond RNA Soil and the DNA Set for NucleoBond RNA Soil. Back NucleoBond RNA Soil Mini provides sufficient RNA yield for qPCR analysis NucleoBond RNA Soil Mini provides sufficient RNA yield for qPCR analysis. 2 different soil samples were purified in duplicate according to the standard protocols for NucleoBond RNA Soil Mini and NucleoBond RNA Soil, respectively. RNA was eluted in 100 µl and quantified photometrically. 15 µl of each eluate was analyzed by electrophoresis on a 1% TAE gel. Yields obtained with NucleoBond RNA Soil Mini were approximately 3–4 times lower than those obtained with NucleoBond RNA Soil, but were well-suited for downstream applications such as qPCR analysis.

DNA Set for NucleoBond RNA Soil Mini is a 50-prep elution set designed to be used in conjunction with NucleoBond RNA Soil Mini (Cat. # 740142.50) for parallel isolation of DNA and RNA from the same soil sample. Nucleic acids purified with these kits are suitable for metagenomic studies, including genomic and transcriptomic analyses. DNA Set for NucleoBond RNA Soil Mini typically yields 1.25–12.5 µg of pure DNA from 0.25–0.5 g of soil. The kit includes sufficient quantities of reagents and materials for processing of 50 samples.

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NucleoBond RNA Soil Mini enables efficient parallel DNA isolation when paired with the DNA Set for NucleoBond RNA Soil Mini

NucleoBond RNA Soil Mini enables efficient parallel DNA isolation when paired with the DNA Set for NucleoBond RNA Soil Mini. Two different soil samples were purified in duplicate according to the standard protocols for NucleoBond RNA Soil Mini and NucleoBond RNA Soil, respectively. DNA was eluted in 100 µl and measured photometrically. 15 µl of each eluate was analyzed on a 1% TAE gel. DNA yields obtained using NucleoBond RNA Soil Mini in tandem with the DNA Set for NucleoBond RNA Soil Mini were approximately 3–4 times lower in comparison to DNA yields obtained with NucleoBond RNA Soil and the DNA Set for NucleoBond RNA Soil.

Required Products

Cat. #	Product	Size	Price	License	Quantity	Details
740142.50	NucleoBond® RNA Soil Mini	50 Preps	$654.00
The NucleoBond RNA Soil Mini kit employs anion exchange chromatography and silica spin purification for isolation of RNA from soil and sediment samples, and can be used in combination with the DNA Set for NucleoBond RNA Soil Mini to obtain both DNA and RNA from the same sample. Nucleic acids purified with these kits are suitable for metagenomic studies, including genomic and transcriptomic analyses. Most soils contain relatively low amounts of RNA compared to DNA. Therefore, this kit was designed to accommodate sample inputs of 0.25–0.5 g in order to yield 0.25–2.5 µg of RNA. Sample lysis using NucleoSpin Bead Tubes Type A in the presence of the optimized Lysis Buffer E1 and phenol:chloroform:isoamyl alcohol ensures efficient sample disruption. The optional Buffer OPT enables efficient RNA and DNA purification from mineral and organic soil samples. NucleoBond RNA Soil Mini enables manual processing of 12 samples in approximately 1 hour. The kit includes sufficient quantities of reagents and materials for processing of 50 samples. Documents You May Also Like Image Data Back NucleoBond RNA Soil Mini enables efficient parallel DNA isolation when paired with the DNA Set for NucleoBond RNA Soil Mini NucleoBond RNA Soil Mini enables efficient parallel DNA isolation when paired with the DNA Set for NucleoBond RNA Soil Mini. Two different soil samples were purified in duplicate according to the standard protocols for NucleoBond RNA Soil Mini and NucleoBond RNA Soil, respectively. DNA was eluted in 100 µl and measured photometrically. 15 µl of each eluate was analyzed on a 1% TAE gel. DNA yields obtained using NucleoBond RNA Soil Mini in tandem with the DNA Set for NucleoBond RNA Soil Mini were approximately 3–4 times lower in comparison to DNA yields obtained with NucleoBond RNA Soil and the DNA Set for NucleoBond RNA Soil. Back NucleoBond RNA Soil Mini provides sufficient RNA yield for qPCR analysis NucleoBond RNA Soil Mini provides sufficient RNA yield for qPCR analysis. 2 different soil samples were purified in duplicate according to the standard protocols for NucleoBond RNA Soil Mini and NucleoBond RNA Soil, respectively. RNA was eluted in 100 µl and quantified photometrically. 15 µl of each eluate was analyzed by electrophoresis on a 1% TAE gel. Yields obtained with NucleoBond RNA Soil Mini were approximately 3–4 times lower than those obtained with NucleoBond RNA Soil, but were well-suited for downstream applications such as qPCR analysis.

The NucleoBond RNA Soil kit employs anion exchange chromatography and silica spin purification for isolation of RNA from soil and sediment samples, and can be used in combination with the DNA Set for NucleoBond RNA Soil to obtain both DNA and RNA from the same sample. Nucleic acids purified with these kits are suitable for metagenomic studies, including genomic and transcriptomic analyses. Most soils contain relatively low amounts of RNA compared to DNA. Therefore, this kit was designed to accomodate high sample inputs in order to yield 1–10 µg of RNA. Sample lysis using NucleoSpin Bead Tubes Type A in the presence of the optimized Lysis Buffer E1 and phenol:chloroform:isoamyl alcohol ensures efficient sample disruption. The optional Buffer OPT enables efficient RNA and DNA purification from mineralic and organic soil samples. NucleoBond RNA Soil enables manual processing of 12 samples in approximately 1 hour. The kit includes sufficient quantities of reagents and materials for processing of 20 samples.

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NucleoBond RNA Soil procedure

NucleoBond RNA Soil procedure.

Back

High RNA yields with NucleoBond RNA Soil

High RNA yields with NucleoBond RNA Soil. Various soil samples (clay, shore soil, and forest soil) were purified in duplicate with NucleoBond RNA Soil using the standard kit protocol. For comparison, the samples were processed in parallel using competitor kit "Q" and the corresponding kit protocol. Each RNA sample was eluted in a volume of 100 µl and yields were determined photometrically (left panel). In addition, 15 µl of each eluate was analyzed by gel electrophoresis (1% TAE; right panel). For each sample type, NucleoBond RNA Soil provided a higher yield compared to the competitor kit.

Back

Amplifiable RNA for perfect results with NucleoBond RNA Soil

Amplifiable RNA for perfect results with NucleoBond RNA Soil. Various soil samples (clay, shore soil, and forest soil) were purified in duplicate using NucleoBond RNA Soil and competitor kit "Q" by following the standard protocol for each kit. RT-PCR was then performed using 40 µl of eluate from each sample (amplicon size = 466 bp). Samples prepared using NucleoBond RNA Soil demonstrated lower Cp values relative to corresponding samples prepared using kit "Q".

Back

NucleoBond RNA Soil enables efficient parallel DNA isolation

NucleoBond RNA Soil enables efficient parallel DNA isolation. Various soil samples (clay, shore soil, and forest soil) were purified in duplicate using NucleoBond RNA Soil in combination with the DNA Set for NucleoBond RNA Soil following the standard kit protocol. For comparison, the samples were processed in parallel using competitor kit "Q" and the corresponding protocol. DNA samples were eluted in a volume of 100 µl and yields were determined photometrically (left panel). In addition, 15 µl of each eluate was analyzed by gel electrophoresis (1% TAE; right panel). For each sample type, NucleoBond RNA Soil combined with the DNA Set for NucleoBond RNA Soil provided a higher yield of DNA compared to the competitor kit.

The NucleoBond RNA Soil Mini kit employs anion exchange chromatography and silica spin purification for isolation of RNA from soil and sediment samples, and can be used in combination with the DNA Set for NucleoBond RNA Soil Mini to obtain both DNA and RNA from the same sample. Nucleic acids purified with these kits are suitable for metagenomic studies. including genomic and transcriptomic analyses. Most soils contain relatively low amounts of RNA compared to DNA. Therefore, this kit was designed to accommodate sample inputs of 0.25–0.5 g in order to yield 0.25–2.5 µg of RNA. Sample lysis using NucleoSpin Bead Tubes Type A in the presence of the optimized Lysis Buffer E1 and phenol:chloroform:isoamyl alcohol ensures efficient sample disruption. The optional Buffer OPT enables efficient RNA and DNA purification from mineral and organic soil samples. NucleoBond RNA Soil Mini enables manual processing of 12 samples in approximately 1 hour. The kit includes sufficient quantities of reagents and materials for processing of 10 samples.

Back

NucleoBond RNA Soil Mini enables efficient parallel DNA isolation when paired with the DNA Set for NucleoBond RNA Soil Mini

NucleoBond RNA Soil Mini enables efficient parallel DNA isolation when paired with the DNA Set for NucleoBond RNA Soil Mini. Two different soil samples were purified in duplicate according to the standard protocols for NucleoBond RNA Soil Mini and NucleoBond RNA Soil, respectively. DNA was eluted in 100 µl and measured photometrically. 15 µl of each eluate was analyzed on a 1% TAE gel. DNA yields obtained using NucleoBond RNA Soil Mini in tandem with the DNA Set for NucleoBond RNA Soil Mini were approximately 3–4 times lower in comparison to DNA yields obtained with NucleoBond RNA Soil and the DNA Set for NucleoBond RNA Soil.

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NucleoBond RNA Soil Mini provides sufficient RNA yield for qPCR analysis

NucleoBond RNA Soil Mini provides sufficient RNA yield for qPCR analysis. 2 different soil samples were purified in duplicate according to the standard protocols for NucleoBond RNA Soil Mini and NucleoBond RNA Soil, respectively. RNA was eluted in 100 µl and quantified photometrically. 15 µl of each eluate was analyzed by electrophoresis on a 1% TAE gel. Yields obtained with NucleoBond RNA Soil Mini were approximately 3–4 times lower than those obtained with NucleoBond RNA Soil, but were well-suited for downstream applications such as qPCR analysis.

The NucleoBond RNA Soil Mini kit employs anion exchange chromatography and silica spin purification for isolation of RNA from soil and sediment samples, and can be used in combination with the DNA Set for NucleoBond RNA Soil Mini to obtain both DNA and RNA from the same sample. Nucleic acids purified with these kits are suitable for metagenomic studies, including genomic and transcriptomic analyses. Most soils contain relatively low amounts of RNA compared to DNA. Therefore, this kit was designed to accommodate sample inputs of 0.25–0.5 g in order to yield 0.25–2.5 µg of RNA. Sample lysis using NucleoSpin Bead Tubes Type A in the presence of the optimized Lysis Buffer E1 and phenol:chloroform:isoamyl alcohol ensures efficient sample disruption. The optional Buffer OPT enables efficient RNA and DNA purification from mineral and organic soil samples. NucleoBond RNA Soil Mini enables manual processing of 12 samples in approximately 1 hour. The kit includes sufficient quantities of reagents and materials for processing of 50 samples.

Back

NucleoBond RNA Soil Mini enables efficient parallel DNA isolation when paired with the DNA Set for NucleoBond RNA Soil Mini

NucleoBond RNA Soil Mini enables efficient parallel DNA isolation when paired with the DNA Set for NucleoBond RNA Soil Mini. Two different soil samples were purified in duplicate according to the standard protocols for NucleoBond RNA Soil Mini and NucleoBond RNA Soil, respectively. DNA was eluted in 100 µl and measured photometrically. 15 µl of each eluate was analyzed on a 1% TAE gel. DNA yields obtained using NucleoBond RNA Soil Mini in tandem with the DNA Set for NucleoBond RNA Soil Mini were approximately 3–4 times lower in comparison to DNA yields obtained with NucleoBond RNA Soil and the DNA Set for NucleoBond RNA Soil.

Back

NucleoBond RNA Soil Mini provides sufficient RNA yield for qPCR analysis

NucleoBond RNA Soil Mini provides sufficient RNA yield for qPCR analysis. 2 different soil samples were purified in duplicate according to the standard protocols for NucleoBond RNA Soil Mini and NucleoBond RNA Soil, respectively. RNA was eluted in 100 µl and quantified photometrically. 15 µl of each eluate was analyzed by electrophoresis on a 1% TAE gel. Yields obtained with NucleoBond RNA Soil Mini were approximately 3–4 times lower than those obtained with NucleoBond RNA Soil, but were well-suited for downstream applications such as qPCR analysis.