Fast and reliable isolation of environmental DNA—NucleoSpin eDNA Water

Isolation of genomic DNA from stool samples

Analysis of environmental DNA (eDNA) can provide a deeper understanding of freshwater and saltwater ecosystems, but common methods for eDNA isolation can be lengthy and laborious, often involving overnight incubation steps. The NucleoSpin eDNA Water kit is designed to streamline eDNA isolation workflows by providing a versatile solution that can be applied to filtered or unfiltered samples, featuring a protocol that can be completed in a little over one hour.

The NucleoSpin eDNA Water workflow has been optimized for speed and simplicity without compromising the quality and purity of isolated DNA. Samples can be obtained by either filtration with round filters or filter cartridges. DNA is liberated from the filter by a strong lysis buffer. Alternatively, eDNA can be precipitated from <40 ml of unfiltered water (protocol included; requires additional buffer PREC). NucleoTrap Bead suspension efficiently binds the DNA, while minimizing carryover of contaminants. Following washing and drying steps, the bead suspension is transferred to a NucleoSpin XS column, from which pure eDNA is eluted. The purification workflow takes less than 70 min, while the full preparation time depends on the sampling and/or filtration setup.

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Cat. #	Product	Size	Price
740402.10	NucleoSpin® eDNA Water	10 Preps	USD $163.00
The NucleoSpin eDNA Water kit enables rapid purification of environmental DNA (eDNA) from diverse water samples with reliable results and minimal risk of DNA contamination. The combination of silica matrix and silica membrane technologies allows for efficient isolation of high-quality DNA in about an hour, providing a convenient alternative to the overnight incubation steps typical of eDNA workflows. The XS columns provided with the kit are treated with ethylene oxide in order to minimize the risk of DNA contamination. NucleoSpin eDNA Water is compatible with a variety of filters and filtration systems, such as conventional bottle top filters (e.g., 45 mm) as well as cartridge filters (e.g., Sterivex). Further, an alternative protocol for direct eDNA precipitation from unfiltered water is provided, enabling isolation of free DNA. These properties make NucleoSpin eDNA Water ideal for studies of freshwater and saltwater ecosystems with varying levels of organic and inorganic matter. Cat. # 740402.10 includes reagents and materials for processing 10 samples. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back 740402.10: NucleoSpin eDNA Water Back Compatibility of NucleoSpin eDNA Water with different water filtration systems Compatibility of NucleoSpin eDNA Water with different water filtration systems. Samples of creek water were filtered through a glass fiber filter (GF) as well as several filtration systems commonly used in conjunction with eDNA purification. The NucleoSpin eDNA Water kit was then used to purify eDNA from each filter unit, and eluates were analyzed by qPCR for metazoan DNA. Purification from each filtration system yielded comparable results, as demonstrated by the consistent Ct values. Back Consistent performance of NucleoSpin eDNA Water with diverse round filters Consistent performance of NucleoSpin eDNA Water with diverse round filters. 500-ml samples of creek water were filtered through the following different types of round filters, which were then processed using NucleoSpin eDNA Water: regenerated cellulose (RC), cellulose nitrate (NC), cellulose acetate (CA), polyester (PE), polycarbonate (PC), mixed cellulose ester (CM) and paper. As demonstrated by the resulting Ct values obtained in a downstream qPCR analysis, performance of DNA isolation was similar for each of the filter types and comparable to the performance observed with the recommended ethylene oxide-treated glass fiber filter (GF), which provides the best flow rate and lowest risk of clogging. Back Sample preparation workflow for NucleoSpin eDNA Water Sample preparation workflow for NucleoSpin eDNA Water. Samples can be obtained either by filtration with round filters or filter cartridges (e.g., Sterivex). DNA is liberated from the filter by a strong lysis buffer. Alternatively, eDNA can be precipitated from <40 ml of unfiltered water (using Buffer PREC, Cat. # 740568). NucleoTrap Suspension beads efficiently bind the DNA while minimizing carryover of contaminants. Following washing and drying steps, the bead suspension is transferred to a NucleoSpin XS column, from which pure eDNA is eluted. The purification steps take less than 70 min, while total preparation times vary depending on the sampling and/or filtration setup. Back Seasonal traceability of European toad in a freshwater pond Seasonal traceability of European toad in a freshwater pond. In Germany, European toads become active after hibernation—usually in March—and typically start spawning in April. The presence of toads and/or spawn and tadpoles can be monitored by isolation of eDNA and subsequent PCR analysis. The NucleoSpin eDNA Water kit was used in combination with NucleoSpin Filter Midi to isolate DNA from a freshwater pond at the indicated timepoints, and PCR was performed to detect toad-specific or general eukaryotic DNA using corresponding primer pairs. Back Superior downstream performance with NucleoSpin eDNA Water as compared to competitor kits Superior downstream performance with NucleoSpin eDNA Water as compared to competitor kits. eDNA was purified from running water (creek) and static water (pond) using the NucleoSpin eDNA Water kit in parallel with two competitor kits ("BT" and "PW") and analyzed by qPCR for metazoan DNA. As demonstrated by the lower Ct values, NucleoSpin eDNA Water provided higher sensitivity than the competitors.
740402.50	NucleoSpin® eDNA Water	50 Preps	USD $635.00
The NucleoSpin eDNA Water kit enables rapid purification of environmental DNA (eDNA) from diverse water samples with reliable results and minimal risk of DNA contamination. The combination of silica matrix and silica membrane technologies allows for efficient isolation of high-quality DNA in about an hour, providing a convenient alternative to the overnight incubation steps typical of eDNA workflows. The XS columns provided with the kit are treated with ethylene oxide in order to minimize the risk of DNA contamination. NucleoSpin eDNA Water is compatible with a variety of filters and filtration systems, such as conventional bottle top filters (e.g., 45 mm) as well as cartridge filters (e.g., Sterivex). Further, an alternative protocol for direct eDNA precipitation from unfiltered water is provided, enabling isolation of free DNA. These properties make NucleoSpin eDNA Water ideal for studies of freshwater and saltwater ecosystems with varying levels of organic and inorganic matter. Cat. # 740402.50 includes reagents and materials for processing 50 samples. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back 740402.50: NucleoSpin eDNA Water Back Compatibility of NucleoSpin eDNA Water with different water filtration systems Compatibility of NucleoSpin eDNA Water with different water filtration systems. Samples of creek water were filtered through a glass fiber filter (GF) as well as several filtration systems commonly used in conjunction with eDNA purification. The NucleoSpin eDNA Water kit was then used to purify eDNA from each filter unit, and eluates were analyzed by qPCR for metazoan DNA. Purification from each filtration system yielded comparable results, as demonstrated by the consistent Ct values. Back Consistent performance of NucleoSpin eDNA Water with diverse round filters Consistent performance of NucleoSpin eDNA Water with diverse round filters. 500-ml samples of creek water were filtered through the following different types of round filters, which were then processed using NucleoSpin eDNA Water: regenerated cellulose (RC), cellulose nitrate (NC), cellulose acetate (CA), polyester (PE), polycarbonate (PC), mixed cellulose ester (CM) and paper. As demonstrated by the resulting Ct values obtained in a downstream qPCR analysis, performance of DNA isolation was similar for each of the filter types and comparable to the performance observed with the recommended ethylene oxide-treated glass fiber filter (GF), which provides the best flow rate and lowest risk of clogging. Back Sample preparation workflow for NucleoSpin eDNA Water Sample preparation workflow for NucleoSpin eDNA Water. Samples can be obtained either by filtration with round filters or filter cartridges (e.g., Sterivex). DNA is liberated from the filter by a strong lysis buffer. Alternatively, eDNA can be precipitated from <40 ml of unfiltered water (using Buffer PREC, Cat. # 740568). NucleoTrap Suspension beads efficiently bind the DNA while minimizing carryover of contaminants. Following washing and drying steps, the bead suspension is transferred to a NucleoSpin XS column, from which pure eDNA is eluted. The purification steps take less than 70 min, while total preparation times vary depending on the sampling and/or filtration setup. Back Seasonal traceability of European toad in a freshwater pond Seasonal traceability of European toad in a freshwater pond. In Germany, European toads become active after hibernation—usually in March—and typically start spawning in April. The presence of toads and/or spawn and tadpoles can be monitored by isolation of eDNA and subsequent PCR analysis. The NucleoSpin eDNA Water kit was used in combination with NucleoSpin Filter Midi to isolate DNA from a freshwater pond at the indicated timepoints, and PCR was performed to detect toad-specific or general eukaryotic DNA using corresponding primer pairs. Back Superior downstream performance with NucleoSpin eDNA Water as compared to competitor kits Superior downstream performance with NucleoSpin eDNA Water as compared to competitor kits. eDNA was purified from running water (creek) and static water (pond) using the NucleoSpin eDNA Water kit in parallel with two competitor kits ("BT" and "PW") and analyzed by qPCR for metazoan DNA. As demonstrated by the lower Ct values, NucleoSpin eDNA Water provided higher sensitivity than the competitors.

Overview

Fast workflow—pure eDNA in hours instead of days
Ethylene oxide-treated XS columns—minimal risk of DNA contamination
Compatible with diverse types of water, filtration systems, and laboratory setups

More Information

Technology	Silica membrane and silica matrix
Starting material	Filter, water
Format	XS spin columns
Handling	Centrifugation (manual)
Fragment size	100 bp–approximately 50 kb
Elution volume	100 µl
Preparation time	<70 min (excluding water filtration)

Applications

Purification of environmental DNA (eDNA) from filters/filter cartridges or unpurified water
Typical downstream applications: qPCR, NGS

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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Fast and reliable isolation of environmental DNA—NucleoSpin eDNA Water

Notice to purchaser

740402.10: NucleoSpin eDNA Water

Compatibility of NucleoSpin eDNA Water with different water filtration systems

Consistent performance of NucleoSpin eDNA Water with diverse round filters

Sample preparation workflow for NucleoSpin eDNA Water

Seasonal traceability of European toad in a freshwater pond

Superior downstream performance with NucleoSpin eDNA Water as compared to competitor kits