High-throughput DNA extraction from stool samples—NucleoSpin 96 DNA Stool
NucleoSpin 96 DNA Stool enables high-throughput isolation of both microbial and host genomic DNA from fresh and frozen stool samples in a 96-well format. The kit employs a special lysis buffer in combination with mechanical sample disruption using MN Bead Tubes Type A (ceramic beads; included in the kit) to achieve efficient sample homogenization without enzymatic reactions such as protease digestion. Automation of the kit protocol is possible on common liquid handling platforms.
NucleoSpin 96 DNA Stool enables high-throughput isolation of both microbial and host genomic DNA from fresh and frozen stool samples in a 96-well format. The kit employs a special lysis buffer in combination with mechanical sample disruption using MN Bead Tubes Type A (ceramic beads; included in the kit) to achieve efficient sample homogenization without enzymatic reactions such as protease digestion. Automation of the kit protocol is possible on common liquid handling platforms.
Sample lysate is cleared in two steps: precipitation in Lysis Buffer ST2 at 2–8°C followed by filtration using the NucleoSpin Stool Filter Plate. Upon addition of Binding Buffer ST3, sample DNA is can be efficiently bound to the NucleoSpin Stool Binding Plate. Residual contaminants such as complex polysaccharides, bile salts, and other PCR inhibitors are removed via sequential washing steps. Following a drying step, ready-to-use DNA can be eluted in Elution Buffer SE.
Overview
- High-throughput isolation of microbial and host DNA from a wide array of stool samples in a 96-well format
- Includes MN bead tubes for efficient lysis—compatible with most common disruption devices
- Purified DNA is ready for downstream analysis by qPCR or NGS
- Scripts available for automated processing on common liquid handling platforms
More Information
| Technology | Silica membrane technology combined with MN Bead Tubes Type A |
| Starting material | 100–200 mg of human stool* (fresh or frozen) |
| Format | 96-well plate |
| Handling | Vacuum (manual or automated) |
| Fragment size | 50 bp–approximately 50 kb |
| Typical yield | 3–15 µg (varies by sample and disruption device) |
| A260/A280 |
1.6–1.8 |
| Elution volume | 100–200 µl |
| Preparation time | 90 min/96 preps (automated processing) |
| Binding capacity | 50 µg |
*For animal stool specimens and challenging samples with high amounts of lipids, polysaccharides, or proteins, it is recommended to first attempt processing of smaller inputs (e.g., 50–100 mg of sample material).
Applications
- Purification of microbial and host DNA from fresh and frozen stool samples
- Typical downstream applications: qPCR, NGS, metagenomic analyses
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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