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Home › Products › Nucleic acid purification › Genomic DNA purification kits › Blood and plasma › NucleoSpin cfDNA XS

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Purification of circulating DNA from plasma—NucleoSpin cfDNA XS

EDTA-treated human plasma

Increased amounts of circulating DNA in plasma have been found in a variety of disorders including cancer, autoimmune diseases, infections, certain fetal diseases, trauma, and stroke. Circulating DNA is known to be highly fragmented and low in concentration, which makes it difficult to purify.

Increased amounts of circulating DNA in plasma have been found in a variety of disorders including cancer, autoimmune diseases, infections, certain fetal diseases, trauma, and stroke. Circulating DNA is known to be highly fragmented and low in concentration, which makes it difficult to purify.

NucleoSpin cfDNA XS is designed to isolate fragmented DNA larger than 50 bp from human EDTA blood plasma. The kit overcomes the limitations of conventional kits, namely large membrane diameters associated with large default elution volumes, and a resulting DNA concentration that is too low for direct use as a PCR template. NucleoSpin cfDNA XS allows elution in just 5–20 µl, resulting in highly concentrated DNA.

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Cat. # Product Size License Quantity Details
740900.50 NucleoSpin® cfDNA XS 50 Preps USD $200.00

NucleoSpin cfDNA XS (50) 50 preps for the isolation of circulating DNA from plasma—NucleoSpin cfDNA XS Columns, Collection Tubes, buffers, Proteinase K.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes

NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes

NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes. Panel A. The columns are optimized to hold a very small silica membrane, yet fit inside a standard 1.5 ml microfuge tube. Panel B. The exceptionally small (2.0 mm) diameter of the NucleoSpinXS membrane (Column MN) allows efficient elution in only 5–30 μl. It provides a significant advantage over the small-sample column offered by Competitor Q (Column Q; 4.8 mm diameter), which requires an elution volume of at least 10–30 μl.

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740900.50: NucleoSpin Plasma XS

740900.50: NucleoSpin Plasma XS
740900.250 NucleoSpin® cfDNA XS 250 Preps USD $925.00

NucleoSpin cfDNA XS (250) 250 preps for the isolation of circulating DNA from plasma—NucleoSpin cfDNA XS Columns, Collection Tubes, buffers, Proteinase K.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data

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NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes

NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes

NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes. Panel A. The columns are optimized to hold a very small silica membrane, yet fit inside a standard 1.5 ml microfuge tube. Panel B. The exceptionally small (2.0 mm) diameter of the NucleoSpinXS membrane (Column MN) allows efficient elution in only 5–30 μl. It provides a significant advantage over the small-sample column offered by Competitor Q (Column Q; 4.8 mm diameter), which requires an elution volume of at least 10–30 μl.

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740900.250: NucleoSpin Plasma XS

740900.250: NucleoSpin Plasma XS
740900.10 NucleoSpin® cfDNA XS 10 Preps USD $36.00

NucleoSpin cfDNA XS (10) 10 preps for the isolation of circulating DNA from plasma—NucleoSpin cfDNA XS Columns, Collection Tubes, buffers, Proteinase K.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740900.10: NucleoSpin Plasma XS

740900.10: NucleoSpin Plasma XS

Isolate circulating DNA from plasma NucleoSnap cfDNA
Technical notes and data highlighting the performance of our nucleic acid purification kits Learning center
Purification kits for the the isolation of RNA or DNA from samples containing virus Viral DNA/RNA purification kits

Overview

  • High recovery of fragments >50 bp
  • Elution in only 5 µl
  • Concentrated DNA even from dilute samples
  • DNA is ready to use for real-time PCR

More Information

Technology

Silica membrane
Format XS spin columns
Starting material 240 µl plasma or serum
Fragment size >50 bp
Typical yield 0.1–100 ng per ml plasma, depending on sample
Typical concentration 1.2–12,000 pg/µl
Elution volume 5–20 µl
Preparation time

High-sensitivity procedure: 22–27 min/6 preps
Rapid procedure: 15–20 min/6 preps

Applications

  • Purify circulating DNA from plasma or serum
  • Analyze fetal DNA from maternal plasma
  • Detect tumor markers in plasma

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


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