Purification of circulating DNA from plasma—NucleoSpin Plasma XS

Increased amounts of circulating DNA in plasma have been found in a variety of disorders including cancer, autoimmune diseases, infections, certain fetal diseases, trauma, and stroke. Circulating DNA is known to be highly fragmented and low in concentration, which makes it difficult to purify.

NucleoSpin Plasma XS is designed to isolate fragmented DNA larger than 50 bp from human EDTA blood plasma. The kit overcomes the limitations of conventional kits, namely large membrane diameters associated with large default elution volumes, and a resulting DNA concentration that is too low for direct use as a PCR template. NucleoSpin Plasma XS allows elution in just 5–20 µl, resulting in highly concentrated DNA.

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Cat. #	Product	Size	Price
740900.50	NucleoSpin® cfDNA XS	50 Preps	$192.00
NucleoSpin cfDNA XS (50) 50 preps for the isolation of circulating DNA from plasma—NucleoSpin cfDNA XS Columns, Collection Tubes, buffers, Proteinase K. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes. Panel A. The columns are optimized to hold a very small silica membrane, yet fit inside a standard 1.5 ml microfuge tube. Panel B. The exceptionally small (2.0 mm) diameter of the NucleoSpinXS membrane (Column MN) allows efficient elution in only 5–30 μl. It provides a significant advantage over the small-sample column offered by Competitor Q (Column Q; 4.8 mm diameter), which requires an elution volume of at least 10–30 μl. Back 740900.50: NucleoSpin Plasma XS
740900.250	NucleoSpin® cfDNA XS	250 Preps	$885.00
NucleoSpin cfDNA XS (250) 250 preps for the isolation of circulating DNA from plasma—NucleoSpin cfDNA XS Columns, Collection Tubes, buffers, Proteinase K. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes. Panel A. The columns are optimized to hold a very small silica membrane, yet fit inside a standard 1.5 ml microfuge tube. Panel B. The exceptionally small (2.0 mm) diameter of the NucleoSpinXS membrane (Column MN) allows efficient elution in only 5–30 μl. It provides a significant advantage over the small-sample column offered by Competitor Q (Column Q; 4.8 mm diameter), which requires an elution volume of at least 10–30 μl. Back 740900.250: NucleoSpin Plasma XS
740900.10	NucleoSpin® cfDNA XS	10 Preps	$34.30
NucleoSpin cfDNA XS (10) 10 preps for the isolation of circulating DNA from plasma—NucleoSpin cfDNA XS Columns, Collection Tubes, buffers, Proteinase K. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back 740900.10: NucleoSpin Plasma XS

Overview

High recovery of fragments >50 bp
Elution in only 5 µl
Concentrated DNA even from dilute samples
DNA is ready to use for real-time PCR

More Information

Technology	Silica membrane
Format	XS spin columns
Starting material	240 µl plasma or serum
Fragment size	>50 bp
Typical yield	0.1–100 ng per ml plasma, depending on sample
Typical concentration	1.2–12,000 pg/µl
Elution volume	5–20 µl
Preparation time	High-sensitivity procedure: 22–27 min/6 preps Rapid procedure: 15–20 min/6 preps

Applications

Purify circulating DNA from plasma or serum
Analyze fetal DNA from maternal plasma
Detect tumor markers in plasma

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Products for isolation of genomic DNA

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