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  • Homodimerization systems
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Home › Products › Gene function › iDimerize inducible protein interaction systems › Homodimerization systems

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Learn more about inducing protein-protein interaction with iDimerize Systems iDimerize systems learning center
Learn more about our tet, ProteoTuner, and iDimerize inducible systems Inducible systems

iDimerize Inducible Homodimer System

Inducible homodimerization systems

The iDimerize Inducible Homodimer System brings protein-protein interactions under real-time, small-molecule control. A protein of interest is fused to the DmrB binding domain, and dimerization is induced by adding the B/B Homodimerizer (identical to the AP20187 ligand). Plasmid and lentiviral (Lenti-X) vector formats are available.

The iDimerize Inducible Homodimer System brings protein-protein interactions under real-time, small-molecule control. A protein of interest is fused to the DmrB binding domain, and dimerization is induced by adding the B/B Homodimerizer (identical to the AP20187 ligand). Plasmid and lentiviral (Lenti-X) vector formats are available.

NOTE: While it is possible to use the iDimerize Inducible Homodimer System to induce heterodimerization, you will obtain a mixture of homodimers and heterodimers. To induce heterodimers specifically and exclusively, use the iDimerize Inducible Heterodimer System.

iDimerize Inducible Homodimer Systems (with Tet-On 3G technology)

One challenge of ligand-dependent dimerization experiments is that non-ligand-induced dimerization events may occur if the protein of interest is expressed at high levels. This is especially problematic if the target protein is a membrane protein, because the local concentration can increase quickly due to the limited space on the membrane. We've combined iDimerize and Tet-On 3G technologies to eliminate these unwanted events. First, use doxycycline to optimize the protein's expression to physiologically relevant levels. Then induce dimerization by adding the dimerizer ligand to your culture medium.

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Cat. # Product Size Price License Quantity Details
635088 B/B Washout Ligand 500 uL USD $414.00

License Statement

ID Number  
57 This product is covered by U.S. Patent Nos. 8,173,792 and 9,487,787.
99 iDimerize and Shield1 Technologies: This product is sold under license from ARIAD Pharmaceuticals, Inc., a wholly owned subsidiary of Takeda Pharmaceutical Company Limited, for use only in internal research conducted by the buyer, which license expressly excludes the right to sell or otherwise transfer the product to third parties and is expressly made subject to compliance with Takara Bio USA, Inc.’s general Terms and Conditions of Sale and any other restrictions set forth on the product’s web page. No right is given to use this product or its components for any other purpose including, but not limited to diagnostic, therapeutic and/or prophylactic applications for or in human beings, animals and/or plants, including but not limited to gene and cell therapy applications, whether in vitro, ex vivo or in vivo, in foods, drugs, devices or cosmetics of any kind, or for consumption by or use in connection with or administration or application to humans or veterinary treatment of animals, or to provide any services, including research services, to third parties. For information regarding licenses for uses other than internal research, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

B/B Washout Ligand is a membrane-permeant ligand that dissociates protein interactions induced by the B/B Homodimerizer, which is provided in each of the iDimerize Inducible Homodimer Systems (Cat. Nos. 635068, 635072, 635084, and 635086). The B/B Washout Ligand dissociates these interactions with a T1/2 of ~10 minutes after adding it to target cells treated with B/B Homodimerizer.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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B/B Washout Ligand releases B/B Homodimerizer-induced interactions

B/B Washout Ligand releases B/B Homodimerizer-induced interactions

B/B Washout Ligand releases B/B Homodimerizer-induced interactions. HeLa cells were transfected with a bidirectional vector (Panel A) which expressed two fluorescent proteins (tdTomato and AcGFP1) each fused to a DmrB dimerization domain. The tdTomato protein was additionally tagged with the FLAG epitope (DYKDDDDK) to enable efficient immunoprecipitation. After overnight treatment with 0.1 μM B/B Homodimerizer, transfected cells were lysed and immunoprecipitated using anti-DYKDDDDK beads (Cat. No. 635686) which recognize the FLAG epitope (Panel B). The beads were washed three times with buffer containing both 0.1 μM B/B Homodimerizer and increasing concentrations of B/B Washout Ligand. After washing, the beads were boiled in SDS-sample buffer, centrifuged, and separated on a polyacrylamide gel. The samples were then analyzed via Western blot using an anti-AcGFP1 specific antibody, and an anti-FLAG antibody to detect the tdTomato fusion (Panel C). B/B Washout Ligand efficiently competed for binding to the DmrB tagged proteins, releasing DmrB-AcGFP1 from the beads.

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B/B Washout Ligand can be used to disrupt protein interactions that were induced by the B/B Homodimerizer

B/B Washout Ligand can be used to disrupt protein interactions that were induced by the B/B Homodimerizer
B/B Washout Ligand can be used to disrupt protein interactions that were induced by the B/B Homodimerizer.

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635088: B/B Washout Ligand

635088: B/B Washout Ligand
635086 Lenti-X™ iDimerize™ Inducible Homodimer System (with Tet-On® 3G) Each Inquire for Quotation

License Statement

ID Number  
42 Use of the Tetracycline controllable expression systems (the "Tet Technology") is covered by a series of patents including U.S. Patent # 8383364, # 9181556 , European patents EP # 1954811, #2352833 and corresponding patent claims outside these regions which are proprietary to TET Systems GmbH & Co. KG. Academic research institutions are granted an automatic license with the purchase of this product to use the Tet Technology only for internal, academic research purposes, which license specifically excludes the right to sell, or otherwise transfer, the Tet Technology or its component parts to third parties. Notwithstanding the above, academic and not-for profit research institutions whose research using the Tet Technology is sponsored by for profit organizations, which shall receive ownership to any data and results stemming from the sponsored research, shall need a commercial license agreement from TET Systems in order to use the Tet Technology. In accepting this license, all users acknowledge that the Tet Technology is experimental in nature. TET Systems GmbH & Co. KG makes no warranties, express or implied or of any kind, and hereby disclaims any warranties, representations, or guarantees of any kind as to the Tet Technology, patents, or products. All others are invited to request a license from TET Systems GmbH & Co. KG prior to purchasing these reagents or using them for any purpose. Takara Bio USA, Inc. is required by its licensing agreement to submit a report of all purchasers of the Tet-controllable expression system to TET Systems.

For license information, please contact:
GSF/CEO
TET Systems GmbH & Co. KG,
Im Neuenheimer Feld 582
69120 Heidelberg
Germany
Tel: +49 6221 5880400
Fax: +49 6221 5880404
email: info@tetsystems.com
or use the electronic licensing request form via https://www.tetsystems.com/licensing/
63 Use of this product is covered by one or more of the following U.S. Patent Nos. and corresponding patent claims outside the U.S.: 8,562,966, 8,557,231. This product is intended for research purposes only. It may not be used for (i) any human or veterinary use, including without limitation therapeutic and prophylactic use, (ii) any clinical use, including without limitation diagnostic use, (iii) screening of chemical and/or biological compounds for the identification of pharmaceutically active agents (including but not limited to screening of small molecules), target validation, preclinical testing services, or drug development. Any use of this product for any of the above mentioned purposes requires a license from the Massachusetts Institute of Technology.
89
This Product is protected by one or more patents from the family consisting of: US9945850 and any corresponding patents, divisionals, continuations, patent applications and foreign filings sharing common priority with the same family.
99 iDimerize and Shield1 Technologies: This product is sold under license from ARIAD Pharmaceuticals, Inc., a wholly owned subsidiary of Takeda Pharmaceutical Company Limited, for use only in internal research conducted by the buyer, which license expressly excludes the right to sell or otherwise transfer the product to third parties and is expressly made subject to compliance with Takara Bio USA, Inc.’s general Terms and Conditions of Sale and any other restrictions set forth on the product’s web page. No right is given to use this product or its components for any other purpose including, but not limited to diagnostic, therapeutic and/or prophylactic applications for or in human beings, animals and/or plants, including but not limited to gene and cell therapy applications, whether in vitro, ex vivo or in vivo, in foods, drugs, devices or cosmetics of any kind, or for consumption by or use in connection with or administration or application to humans or veterinary treatment of animals, or to provide any services, including research services, to third parties. For information regarding licenses for uses other than internal research, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.
*

The Lenti-X iDimerize Inducible Homodimer System (with Tet-On 3G) is a tetracycline-inducible lentiviral gene expression system that lets you control the expression level and dimerization of a protein of interest in live cells. The system includes the pLVX-TRE3G-Hom1 Vector Set, which contains a mammalian expression vector (pLVX-TRE3G-Hom1) that allows doxycycline-dependent expression of your gene of interest, tagged with the DmrB domain, in Tet-On 3G transactivator-expressing cells. Homodimerization of the resulting DmrB-tagged protein is controlled by the addition of the B/B Homodimerizer.

The Lenti-X iDimerize Inducible Homodimer System (with Tet-On 3G) includes vectors and linear selection markers, an optimized packaging system, and the B/B Homodimerizer. The system provides enough reagents for 16 lentiviral packaging reactions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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635084 iDimerize™ Inducible Homodimer System (with Tet-On® 3G) Each Inquire for Quotation

License Statement

ID Number  
42 Use of the Tetracycline controllable expression systems (the "Tet Technology") is covered by a series of patents including U.S. Patent # 8383364, # 9181556 , European patents EP # 1954811, #2352833 and corresponding patent claims outside these regions which are proprietary to TET Systems GmbH & Co. KG. Academic research institutions are granted an automatic license with the purchase of this product to use the Tet Technology only for internal, academic research purposes, which license specifically excludes the right to sell, or otherwise transfer, the Tet Technology or its component parts to third parties. Notwithstanding the above, academic and not-for profit research institutions whose research using the Tet Technology is sponsored by for profit organizations, which shall receive ownership to any data and results stemming from the sponsored research, shall need a commercial license agreement from TET Systems in order to use the Tet Technology. In accepting this license, all users acknowledge that the Tet Technology is experimental in nature. TET Systems GmbH & Co. KG makes no warranties, express or implied or of any kind, and hereby disclaims any warranties, representations, or guarantees of any kind as to the Tet Technology, patents, or products. All others are invited to request a license from TET Systems GmbH & Co. KG prior to purchasing these reagents or using them for any purpose. Takara Bio USA, Inc. is required by its licensing agreement to submit a report of all purchasers of the Tet-controllable expression system to TET Systems.

For license information, please contact:
GSF/CEO
TET Systems GmbH & Co. KG,
Im Neuenheimer Feld 582
69120 Heidelberg
Germany
Tel: +49 6221 5880400
Fax: +49 6221 5880404
email: info@tetsystems.com
or use the electronic licensing request form via https://www.tetsystems.com/licensing/
63 Use of this product is covered by one or more of the following U.S. Patent Nos. and corresponding patent claims outside the U.S.: 8,562,966, 8,557,231. This product is intended for research purposes only. It may not be used for (i) any human or veterinary use, including without limitation therapeutic and prophylactic use, (ii) any clinical use, including without limitation diagnostic use, (iii) screening of chemical and/or biological compounds for the identification of pharmaceutically active agents (including but not limited to screening of small molecules), target validation, preclinical testing services, or drug development. Any use of this product for any of the above mentioned purposes requires a license from the Massachusetts Institute of Technology.
99 iDimerize and Shield1 Technologies: This product is sold under license from ARIAD Pharmaceuticals, Inc., a wholly owned subsidiary of Takeda Pharmaceutical Company Limited, for use only in internal research conducted by the buyer, which license expressly excludes the right to sell or otherwise transfer the product to third parties and is expressly made subject to compliance with Takara Bio USA, Inc.’s general Terms and Conditions of Sale and any other restrictions set forth on the product’s web page. No right is given to use this product or its components for any other purpose including, but not limited to diagnostic, therapeutic and/or prophylactic applications for or in human beings, animals and/or plants, including but not limited to gene and cell therapy applications, whether in vitro, ex vivo or in vivo, in foods, drugs, devices or cosmetics of any kind, or for consumption by or use in connection with or administration or application to humans or veterinary treatment of animals, or to provide any services, including research services, to third parties. For information regarding licenses for uses other than internal research, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.
*

The iDimerize Inducible Homodimer System (with Tet-On 3G) lets you control the expression level and dimerization of a protein of interest in live cells. The system includes the iDimerize Inducible Homodimer Vector Set 2, which contains a mammalian expression vector (pTRE3G-Hom1) that allows doxycycline-dependent expression of your protein of interest, tagged with the DmrB domain, in Tet-On 3G transactivator-expressing cells. Homodimerization of the resulting DmrB-tagged protein is controlled by the addition of the B/B Homoodimerizer.

The iDimerize Inducible Homodimer System (with Tet-On 3G) includes vectors and linear selection markers, our highly efficient transfection reagent, Xfect, Tet System Approved FBS, and the B/B Homodimerizer.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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635072 Lenti-X™ iDimerize™ Inducible Homodimer System Each USD $1247.00

License Statement

ID Number  
99 iDimerize and Shield1 Technologies: This product is sold under license from ARIAD Pharmaceuticals, Inc., a wholly owned subsidiary of Takeda Pharmaceutical Company Limited, for use only in internal research conducted by the buyer, which license expressly excludes the right to sell or otherwise transfer the product to third parties and is expressly made subject to compliance with Takara Bio USA, Inc.’s general Terms and Conditions of Sale and any other restrictions set forth on the product’s web page. No right is given to use this product or its components for any other purpose including, but not limited to diagnostic, therapeutic and/or prophylactic applications for or in human beings, animals and/or plants, including but not limited to gene and cell therapy applications, whether in vitro, ex vivo or in vivo, in foods, drugs, devices or cosmetics of any kind, or for consumption by or use in connection with or administration or application to humans or veterinary treatment of animals, or to provide any services, including research services, to third parties. For information regarding licenses for uses other than internal research, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

The Lenti-X iDimerize Inducible Homodimer Vector Set 1 is a component of the Lenti-X iDimerize Inducible Homodimer System (Cat. No. 635072). This system enables lentiviral delivery of your gene of interest into a variety of mammalian cells, and the expression of your protein of interest, tagged with a domain allowing for inducible protein homodimerization, via a small molecule "dimerizer".

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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635069 B/B Homodimerizer 25 mg USD $2654.00

License Statement

ID Number  
99 iDimerize and Shield1 Technologies: This product is sold under license from ARIAD Pharmaceuticals, Inc., a wholly owned subsidiary of Takeda Pharmaceutical Company Limited, for use only in internal research conducted by the buyer, which license expressly excludes the right to sell or otherwise transfer the product to third parties and is expressly made subject to compliance with Takara Bio USA, Inc.’s general Terms and Conditions of Sale and any other restrictions set forth on the product’s web page. No right is given to use this product or its components for any other purpose including, but not limited to diagnostic, therapeutic and/or prophylactic applications for or in human beings, animals and/or plants, including but not limited to gene and cell therapy applications, whether in vitro, ex vivo or in vivo, in foods, drugs, devices or cosmetics of any kind, or for consumption by or use in connection with or administration or application to humans or veterinary treatment of animals, or to provide any services, including research services, to third parties. For information regarding licenses for uses other than internal research, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

The B/B Homodimerizer is a membrane-permeant ligand that causes homodimerization of proteins fused to dimerization domain B (DmrB)—provided in the iDimerize Inducible Homodimer System (Cat. No. 635068). NOTE: The B/B Homodimerizer is identical to the AP20187 ligand previously supplied in the ARGENT Regulated Homodimerization Kit from ARIAD Pharmaceuticals, Inc.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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635069: B/B Homodimerizer

635069: B/B Homodimerizer

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Signal transduction example 2

Signal transduction example 2

Signal transduction example 2. Inducible mouse model for prostate cancer. iDimerize Inducible Homodimer technology was used to characterize the role of different FGF receptor subtypes (FGFR1 and FGFR2) in prostate cancer. Transgenic mice (which express conditionally active alleles of FGFR1 or FGFR2 in prostate tissue) were treated with B/B Homodimerizer and monitored for prostate cancer. FGFR1, but not FGFR2, was found to play a role in prostate cancer development [Freeman, K. W., et al. (2003) Cancer Res. 63(23):8256–8563].

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Signal transduction example 1: Inducing a Programmed Cell Death Pathway (Inducible Apoptosis)

Signal transduction example 1: Inducing a Programmed Cell Death Pathway (Inducible Apoptosis)
Signal transduction example 1: Inducing a Programmed Cell Death Pathway (Inducible Apoptosis). The Fas receptor (FasR) is a transmembrane protein located on the surface of cells that activates programmed cell death (apoptosis) when induced to trimerize by fas ligand (FasL) molecules located on the surface of adjacent cells (e.g., cytotoxic T cells). FasR-FasL binding plays an important role in the regulation of the immune system and cancer progression. This trimerization (and the resulting apoptotic signaling cascade) can be mimicked using the iDimerize Inducible Homodimer System: cells expressing a FasR-DmrB fusion protein can induce cell death on demand, simply by adding the B/B Homodimerizer. An in vivo model [the MaFIA mouse; Burnett, S. H. et al. (2004) J. Leukoc. Biol. 75(4):612–623] utilizes the Fas receptor to systematically and reversibly eliminate macrophages from transgenic mice.

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Molecular weights of iDimerize ligands

Molecular weights of iDimerize ligands
Molecular weights of iDimerize ligands.
635068 iDimerize™ Inducible Homodimer System Each USD $1143.00

License Statement

ID Number  
99 iDimerize and Shield1 Technologies: This product is sold under license from ARIAD Pharmaceuticals, Inc., a wholly owned subsidiary of Takeda Pharmaceutical Company Limited, for use only in internal research conducted by the buyer, which license expressly excludes the right to sell or otherwise transfer the product to third parties and is expressly made subject to compliance with Takara Bio USA, Inc.’s general Terms and Conditions of Sale and any other restrictions set forth on the product’s web page. No right is given to use this product or its components for any other purpose including, but not limited to diagnostic, therapeutic and/or prophylactic applications for or in human beings, animals and/or plants, including but not limited to gene and cell therapy applications, whether in vitro, ex vivo or in vivo, in foods, drugs, devices or cosmetics of any kind, or for consumption by or use in connection with or administration or application to humans or veterinary treatment of animals, or to provide any services, including research services, to third parties. For information regarding licenses for uses other than internal research, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

The iDimerize Inducible Homodimer System lets you control the homodimerization of a protein of interest in live cells via a membrane permeant compound. The system includes the iDimerize Inducible Homodimer Vector Set 1, which contains two mammalian expression vectors encoding fusion tags that can be easily added to your protein of interest. The activity and localization of the resulting chimeric protein can be controlled by the addition of a small molecule to the cell medium. The system also includes the membrane-permeant compound, B/B Homodimerizer, required for homodimerization, and two linear selection markers for hygromycin and puromycin resistance.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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How does iDimerize technology work?

How does iDimerize technology work?

How does iDimerize technology work? A chemical inducer of dimerization, or “dimerizer”, is a cell-permeant organic small molecule with two separate motifs that each bind with high affinity to a specific protein module (Dmr domain) fused onto the protein(s) of interest. Addition of the dimerizer brings the chimeric protein subunits into very close proximity to each other, mimicking the activation of the cellular event that dimerization of interest controls. B/B Homodimerizer induces self-association of two copies of the same protein whereas A/C Heterodimerizer induces association of two different proteins. Conversely, a reverse dimerizer ligand (D/D Solubilizer) will bind to and dissociate a protein that aggregates in its absence.

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Activation of a signal transduction pathway through dimerization of a cell surface receptor protein

Activation of a signal transduction pathway through dimerization of a cell surface receptor protein
Activation of a signal transduction pathway through dimerization of a cell surface receptor protein. Cell surface receptor proteins fused to the DmrB domain do not interact until the B/B Homodimerizer is added. This cell-permeant ligand induces the fusion proteins to interact, activating a downstream event.

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Signal transduction example 2

Signal transduction example 2

Signal transduction example 2. Inducible mouse model for prostate cancer. iDimerize Inducible Homodimer technology was used to characterize the role of different FGF receptor subtypes (FGFR1 and FGFR2) in prostate cancer. Transgenic mice (which express conditionally active alleles of FGFR1 or FGFR2 in prostate tissue) were treated with B/B Homodimerizer and monitored for prostate cancer. FGFR1, but not FGFR2, was found to play a role in prostate cancer development [Freeman, K. W., et al. (2003) Cancer Res. 63(23):8256–8563].

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Signal transduction example 1: Inducing a Programmed Cell Death Pathway (Inducible Apoptosis)

Signal transduction example 1: Inducing a Programmed Cell Death Pathway (Inducible Apoptosis)
Signal transduction example 1: Inducing a Programmed Cell Death Pathway (Inducible Apoptosis). The Fas receptor (FasR) is a transmembrane protein located on the surface of cells that activates programmed cell death (apoptosis) when induced to trimerize by fas ligand (FasL) molecules located on the surface of adjacent cells (e.g., cytotoxic T cells). FasR-FasL binding plays an important role in the regulation of the immune system and cancer progression. This trimerization (and the resulting apoptotic signaling cascade) can be mimicked using the iDimerize Inducible Homodimer System: cells expressing a FasR-DmrB fusion protein can induce cell death on demand, simply by adding the B/B Homodimerizer. An in vivo model [the MaFIA mouse; Burnett, S. H. et al. (2004) J. Leukoc. Biol. 75(4):612–623] utilizes the Fas receptor to systematically and reversibly eliminate macrophages from transgenic mice.

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Exploiting fas-mediated apoptosis to induce programmed cell death

Exploiting fas-mediated apoptosis to induce programmed cell death

Exploiting fas-mediated apoptosis to induce programmed cell death. Fas-mediated apoptosis is triggered by trimerization of the fas receptor (FasR) via its interaction with the fas ligand (FasL) on an adjacent cell (left). This FasR trimerization event activates the caspase 8 pathway. In the MaFIA mouse model, the fas death domain is fused to two repeats of a dimerization domain and expressed from a myeloid-specific promoter. Apoptosis of macrophage cells in this mouse is induced by treatment with B/B Homodimerizer (AP20187) (right).

635059 B/B Homodimerizer 5 x 500 uL USD $574.00

License Statement

ID Number  
99 iDimerize and Shield1 Technologies: This product is sold under license from ARIAD Pharmaceuticals, Inc., a wholly owned subsidiary of Takeda Pharmaceutical Company Limited, for use only in internal research conducted by the buyer, which license expressly excludes the right to sell or otherwise transfer the product to third parties and is expressly made subject to compliance with Takara Bio USA, Inc.’s general Terms and Conditions of Sale and any other restrictions set forth on the product’s web page. No right is given to use this product or its components for any other purpose including, but not limited to diagnostic, therapeutic and/or prophylactic applications for or in human beings, animals and/or plants, including but not limited to gene and cell therapy applications, whether in vitro, ex vivo or in vivo, in foods, drugs, devices or cosmetics of any kind, or for consumption by or use in connection with or administration or application to humans or veterinary treatment of animals, or to provide any services, including research services, to third parties. For information regarding licenses for uses other than internal research, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

The B/B Homodimerizer is a membrane-permeant ligand that causes homodimerization of proteins fused to dimerization domain B (DmrB)—provided in the iDimerize Inducible Homodimer System (Cat. No. 635068). NOTE: The B/B Homodimerizer is identical to the AP20187 ligand previously supplied in the ARGENT Regulated Homodimerization Kit from ARIAD Pharmaceuticals, Inc.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Molecular structure of the B/B Homodimerizer

Molecular structure of the B/B Homodimerizer
Molecular structure of the B/B Homodimerizer.

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Molecular weights of iDimerize ligands

Molecular weights of iDimerize ligands
Molecular weights of iDimerize ligands.

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Exploiting fas-mediated apoptosis to induce programmed cell death

Exploiting fas-mediated apoptosis to induce programmed cell death

Exploiting fas-mediated apoptosis to induce programmed cell death. Fas-mediated apoptosis is triggered by trimerization of the fas receptor (FasR) via its interaction with the fas ligand (FasL) on an adjacent cell (left). This FasR trimerization event activates the caspase 8 pathway. In the MaFIA mouse model, the fas death domain is fused to two repeats of a dimerization domain and expressed from a myeloid-specific promoter. Apoptosis of macrophage cells in this mouse is induced by treatment with B/B Homodimerizer (AP20187) (right).

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635059: B/B Homodimerizer

635059: B/B Homodimerizer
635058 B/B Homodimerizer 5 mg USD $700.00

License Statement

ID Number  
99 iDimerize and Shield1 Technologies: This product is sold under license from ARIAD Pharmaceuticals, Inc., a wholly owned subsidiary of Takeda Pharmaceutical Company Limited, for use only in internal research conducted by the buyer, which license expressly excludes the right to sell or otherwise transfer the product to third parties and is expressly made subject to compliance with Takara Bio USA, Inc.’s general Terms and Conditions of Sale and any other restrictions set forth on the product’s web page. No right is given to use this product or its components for any other purpose including, but not limited to diagnostic, therapeutic and/or prophylactic applications for or in human beings, animals and/or plants, including but not limited to gene and cell therapy applications, whether in vitro, ex vivo or in vivo, in foods, drugs, devices or cosmetics of any kind, or for consumption by or use in connection with or administration or application to humans or veterinary treatment of animals, or to provide any services, including research services, to third parties. For information regarding licenses for uses other than internal research, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

The B/B Homodimerizer is a membrane-permeant ligand that causes homodimerization of proteins fused to dimerization domain B (DmrB)—provided in the iDimerize Inducible Homodimer System (Cat. No. 635068). NOTE: The B/B Homodimerizer is identical to the AP20187 ligand previously supplied in the ARGENT Regulated Homodimerization Kit from ARIAD Pharmaceuticals, Inc.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data Resources

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Signal transduction example 2

Signal transduction example 2

Signal transduction example 2. Inducible mouse model for prostate cancer. iDimerize Inducible Homodimer technology was used to characterize the role of different FGF receptor subtypes (FGFR1 and FGFR2) in prostate cancer. Transgenic mice (which express conditionally active alleles of FGFR1 or FGFR2 in prostate tissue) were treated with B/B Homodimerizer and monitored for prostate cancer. FGFR1, but not FGFR2, was found to play a role in prostate cancer development [Freeman, K. W., et al. (2003) Cancer Res. 63(23):8256–8563].

Back

Molecular structure of the B/B Homodimerizer

Molecular structure of the B/B Homodimerizer
Molecular structure of the B/B Homodimerizer.

Back

Signal transduction example 1: Inducing a Programmed Cell Death Pathway (Inducible Apoptosis)

Signal transduction example 1: Inducing a Programmed Cell Death Pathway (Inducible Apoptosis)
Signal transduction example 1: Inducing a Programmed Cell Death Pathway (Inducible Apoptosis). The Fas receptor (FasR) is a transmembrane protein located on the surface of cells that activates programmed cell death (apoptosis) when induced to trimerize by fas ligand (FasL) molecules located on the surface of adjacent cells (e.g., cytotoxic T cells). FasR-FasL binding plays an important role in the regulation of the immune system and cancer progression. This trimerization (and the resulting apoptotic signaling cascade) can be mimicked using the iDimerize Inducible Homodimer System: cells expressing a FasR-DmrB fusion protein can induce cell death on demand, simply by adding the B/B Homodimerizer. An in vivo model [the MaFIA mouse; Burnett, S. H. et al. (2004) J. Leukoc. Biol. 75(4):612–623] utilizes the Fas receptor to systematically and reversibly eliminate macrophages from transgenic mice.

Back

Molecular weights of iDimerize ligands

Molecular weights of iDimerize ligands
Molecular weights of iDimerize ligands.

Back

Exploiting fas-mediated apoptosis to induce programmed cell death

Exploiting fas-mediated apoptosis to induce programmed cell death

Exploiting fas-mediated apoptosis to induce programmed cell death. Fas-mediated apoptosis is triggered by trimerization of the fas receptor (FasR) via its interaction with the fas ligand (FasL) on an adjacent cell (left). This FasR trimerization event activates the caspase 8 pathway. In the MaFIA mouse model, the fas death domain is fused to two repeats of a dimerization domain and expressed from a myeloid-specific promoter. Apoptosis of macrophage cells in this mouse is induced by treatment with B/B Homodimerizer (AP20187) (right).

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635058: B/B Homodimerizer

635058: B/B Homodimerizer
632622 B/B Homodimerizer 4 x 25 mg USD $8438.00

License Statement

ID Number  
99 iDimerize and Shield1 Technologies: This product is sold under license from ARIAD Pharmaceuticals, Inc., a wholly owned subsidiary of Takeda Pharmaceutical Company Limited, for use only in internal research conducted by the buyer, which license expressly excludes the right to sell or otherwise transfer the product to third parties and is expressly made subject to compliance with Takara Bio USA, Inc.’s general Terms and Conditions of Sale and any other restrictions set forth on the product’s web page. No right is given to use this product or its components for any other purpose including, but not limited to diagnostic, therapeutic and/or prophylactic applications for or in human beings, animals and/or plants, including but not limited to gene and cell therapy applications, whether in vitro, ex vivo or in vivo, in foods, drugs, devices or cosmetics of any kind, or for consumption by or use in connection with or administration or application to humans or veterinary treatment of animals, or to provide any services, including research services, to third parties. For information regarding licenses for uses other than internal research, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

The B/B Homodimerizer is a membrane-permeant ligand that causes homodimerization of proteins fused to dimerization domain B (DmrB)-provided in the iDimerize Inducible Homodimer System (Cat. No. 635068). NOTE: The B/B Homodimerizer is identical to the AP20187 ligand previously supplied in the ARGENT Regulated Homodimerization Kit from ARIAD Pharmaceuticals, Inc.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data Resources

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632622: B/B Homodimerizer

632622: B/B Homodimerizer

Back

Signal transduction example 2

Signal transduction example 2

Signal transduction example 2. Inducible mouse model for prostate cancer. iDimerize Inducible Homodimer technology was used to characterize the role of different FGF receptor subtypes (FGFR1 and FGFR2) in prostate cancer. Transgenic mice (which express conditionally active alleles of FGFR1 or FGFR2 in prostate tissue) were treated with B/B Homodimerizer and monitored for prostate cancer. FGFR1, but not FGFR2, was found to play a role in prostate cancer development [Freeman, K. W., et al. (2003) Cancer Res. 63(23):8256–8563].

Back

Signal transduction example 1: Inducing a Programmed Cell Death Pathway (Inducible Apoptosis)

Signal transduction example 1: Inducing a Programmed Cell Death Pathway (Inducible Apoptosis)
Signal transduction example 1: Inducing a Programmed Cell Death Pathway (Inducible Apoptosis). The Fas receptor (FasR) is a transmembrane protein located on the surface of cells that activates programmed cell death (apoptosis) when induced to trimerize by fas ligand (FasL) molecules located on the surface of adjacent cells (e.g., cytotoxic T cells). FasR-FasL binding plays an important role in the regulation of the immune system and cancer progression. This trimerization (and the resulting apoptotic signaling cascade) can be mimicked using the iDimerize Inducible Homodimer System: cells expressing a FasR-DmrB fusion protein can induce cell death on demand, simply by adding the B/B Homodimerizer. An in vivo model [the MaFIA mouse; Burnett, S. H. et al. (2004) J. Leukoc. Biol. 75(4):612–623] utilizes the Fas receptor to systematically and reversibly eliminate macrophages from transgenic mice.

Back

Exploiting fas-mediated apoptosis to induce programmed cell death

Exploiting fas-mediated apoptosis to induce programmed cell death

Exploiting fas-mediated apoptosis to induce programmed cell death. Fas-mediated apoptosis is triggered by trimerization of the fas receptor (FasR) via its interaction with the fas ligand (FasL) on an adjacent cell (left). This FasR trimerization event activates the caspase 8 pathway. In the MaFIA mouse model, the fas death domain is fused to two repeats of a dimerization domain and expressed from a myeloid-specific promoter. Apoptosis of macrophage cells in this mouse is induced by treatment with B/B Homodimerizer (AP20187) (right).

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A/C Heterodimerizer, B/B Homodimeirzer, AP20187, AP21967 Dimerizer ligands
Indible heterodimer system using AP21967 ligand Induce heterodimers
Citations Published studies

Overview

  • Previously available from ARIAD as the ARGENT Regulated Homodimerization Kit
  • Rapid chemical induction of cell signaling pathways
  • Activate any pathway or cellular event controlled by homo-oligomers (e.g., dimers, trimers, or tetramers)
  • Established tool used by over 2,000 research groups, with over 400 scientific publications to date
  • B/B Homodimerizer is identical to the AP20187 ligand
  • B/B Washout Ligand reverses protein oligomers that were induced by the B/B Homodimerizer
  • Plasmid and lentiviral (Lenti-X) vector formats are available

More Information

Applications

  • Induce any event or pathway that is controlled or activated by protein-protein interactions
  • Activate signaling pathways in real time
  • Study transmembrane receptors
  • Recruit signaling proteins to specific subcellular locations (e.g., the plasma membrane)
  • Inducible homodimer technology has been used for functional studies of:
    • Receptor and non-receptor tyrosine kinases
    • Receptor and non-receptor serine/threonine kinases
    • Non-kinase receptors
    • Signaling proteases
    • Adaptor proteins
    • Cell adhesion and rolling
    • DNA looping
    • Activation of recombinase
    • RNA splicing
    • Protein splicing
    • Glycosylation
Product citations

Burnett, S. H. et al. Conditional macrophage ablation in transgenic mice expressing a Fas-based suicide gene. J. Leukoc. Biol. 75, 612–623 (2004).

Freeman, K. W. et al. Inducible prostate intraepithelial neoplasia with reversible hyperplasia in conditional FGFR1-expressing mice. Cancer Res. 63, 8256–63 (2003).               

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


Control protein interactions using dimerizer ligands such as AP20187 and AP21967

Instant control of protein-protein interactions in mammalian cells

Every process in the cell is regulated by a complex network of protein-protein interactions. iDimerize technology allows you to specifically, rapidly, and reversibly control the interaction between two proteins simply by adding or removing a small molecule ligand from the culture medium.

iDimerize learning center Technology overview

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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