Tetracycline inducible expression—Tet-One systems
Before the Tet-One systems were developed, our Tet-On and Tet-Off products all required two separate vectors: one to introduce the doxycycline-responsive transactivator protein into your target cells, and the other to introduce the inducible promoter controlling your gene of interest.
Before the Tet-One systems were developed, our Tet-On and Tet-Off products all required two separate vectors: one to introduce the doxycycline-responsive transactivator protein into your target cells, and the other to introduce the inducible promoter controlling your gene of interest.
The Tet-One Systems provide both of these components on a single vector. The Tet-On 3G transactivator is expressed in the forward direction from the human phosphoglycerate kinase 1 promoter, and the cloned gene of interest is expressed from the PTRE3GS promoter in the reverse orientation. Compared to the two-vector Tet-On 3G systems, all previously published all-in-one vectors have shown a low signal-to-noise ratio, typically providing only 50- to 100-fold induced expression, even in selected clones. Our Tet-One systems are based on an all-in-one design that has shown up to 25,000-fold induction (Heinz et al. 2011).
Overview
- All-in-one vector design provides all the power of Tet-On 3G technology in a single vector, making inducible expression as easy as constitutive expression
- Low background and high maximum induced expression
- High sensitivity to doxycycline: near maximum expression at 10 ng/ml doxycycline
- Inducible expression in a broad range of tissues, because the Tet-On 3G transactivator is expressed from the human PGK promoter
- High-titer lentivirus packaging system provided with Lenti-X Tet-One systems
- Retro-X systems incude our Retro-X Universal Packaging System
- The AAVpro system uses a helper-free packaging system and includes extraction reagents for efficient isolation of AAV particles
More Information
Applications
- Tightly controlled inducible expression in mammalian cells
References
Heinz, N., Schambach, A., Galla, M., Maetzig, T., Baum, C., Loew R. & Schiedlmeier, B. Retroviral and transposon-based Tet-regulated all-in-one vectors with reduced background expression and improved dynamic range. Human Gene Ther. 22, 166–176 (2011).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Tighly controlled expression system that is as easy to use as any constitutive system
Until now, the best inducible mammalian expression systems have all required a two-vector format. Tet-One inducible expression systems are the only commercially available single-vector systems that can deliver a >10,000-fold difference in expression levels between induced and uninduced states.
Tet-One technology overview All inducible systemsTakara Bio USA, Inc.
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