Retro-X™ Tet-One™ Inducible Expression System (Puro)

Recommended Products

Cat. # Product Size Price License Quantity Details
631453 Retro-X™ qRT-PCR Titration Kit 200 Rxns USD $579.00

This Retro-X qRT-PCR Titration Kit and a real-time PCR instrument can be used to rapidly quantify the virion content of MMLV-based retroviral supernatants. The viral genome copy number in a supernatant sample is determined by first purifying the viral RNA, and then comparing the RT-PCR threshold cycle (Ct) of an appropriate dilution of purified viral RNA to sample curve Ct values that are derived from a calibrated, serially diluted control viral RNA template.

631530 Retro-X™ Universal Packaging System Each USD $941.00

This kit provides a complete retroviral vector packaging system for use with any retroviral vector. It is designed to produce virus that can infect dividing cells from a broad range of mammalian and nonmammalian cell types. The GP2-293 Packaging Cell Line contains only the MoMuLV gag and pol genes. The viral envelope portion of the packaging function (env gene) is supplied by transiently co-transfecting one of the Retro-X Universal Packaging Vectors (p10A1, pAmpho, pEco, or pVSV-G) with the retroviral vector. The pQCLIN Retroviral Vector (included in the vector set) can be used as a positive control to constitutively express β-galactosidase in transduced mammalian cells.

631455 Retro-X™ Concentrator 100 mL USD $271.00

The Retro-X Concentrator is a complete reagent for the concentration of infectious retroviral vector particles. This reagent provides a scalable alternative to ultracentrifugation for viral particle concentration. Vector supernatants can be concentrated 10–100 fold, depending upon the volumes and envelope used.

T100A RetroNectin® Recombinant Human Fibronectin Fragment 0.5 mg USD $229.00

RetroNectin reagent is a 63-kD fragment of recombinant human fibronectin fragment (also referred to as rFN-CH-296) that enhances the efficiency of lentiviral- and retroviral-mediated gene transduction. This is particularly important for hematopoietic cells and other hard-to-transfect cell types. When coated on the surface of containers such as culture dishes, petri dishes, flasks or bags, RetroNectin significantly enhances virus-mediated gene transduction into mammalian cells. This enhanced transduction is thought to result from the colocalization of virus particles and target cells on the molecules of RetroNectin. Viral particles bind RetroNectin via interaction with heparin-binding domain II, and target cells bind mainly through the interaction of the cell surface integrin receptor VLA-4 with the fibronectin CS1 site.

0027VCT rLV.EF1.Tet3G-9 3 x 20 uL USD $2177.00

The Flash Therapeutics Inducible Reprogramming Lentiviral Vectors are prepackaged lentiviral particles expressing polycistronic Oct4, Sox2, KLF4, and Myc for reprogramming human somatic cells under the induction of doxycycline using Tet technology. These VSV-G pseudotyped viruses are capable of infecting both dividing and non-dividing cells. The high purification level of these lentiviral particles allows transduction of primary cells and immortalized cell lines without inducing any toxicity to the cells, even at high multiplicity of infection (M.O.I.). The expression of these four factors has been shown to reprogram a variety of differentiated cells into induced pluripotent stem (iPS) cells with embryonic stem (ES) cell-like properties.

631132 TetR Monoclonal Antibody (Clone 9G9) 200 ug USD $467.00

Monoclonal antibody produced from mouse hybridoma cells against the wild-type bacterial Tet repressor (TetR). It can be used for detecting TetR-containing proteins on Western blots, and for testing or screening cell lines expressing the Tet-On Advanced, Tet-Off Advanced, or Tet-Off transactivators. It is not recommended for cell lines expressing the original Tet-On or Tet-tTS proteins.