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  • Fluorescent protein plasmids
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Selection guides Fluorescent lentiviral particles
Antibodies to GFP variants, mCherry and other fluorescent proteins Fluorescent protein antibodies
Home › Products › Gene function › Fluorescent proteins › Subcellular labeling plasmids

Gene function

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Selection guides Fluorescent lentiviral particles
Antibodies to GFP variants, mCherry and other fluorescent proteins Fluorescent protein antibodies

Fluorescent proteins for organelle and subcellular labeling

Label subcellular organelles using fluorescent proteins

Visualize subcellular structures directly and noninvasively by fluorescence microscopy. With these vectors, you can study cytoskeletal and organelle structure and function in living cells, in real time, and without chemical staining. You can monitor the location of a protein of interest relative to a given subcellular structure by labeling both the protein and the structure with separate fluorescent proteins. Ready-made lentiviral particles are also available for this application.

Visualize subcellular structures directly and noninvasively by fluorescence microscopy. With these vectors, you can study cytoskeletal and organelle structure and function in living cells, in real time, and without chemical staining. You can monitor the location of a protein of interest relative to a given subcellular structure by labeling both the protein and the structure with separate fluorescent proteins. Ready-made lentiviral particles are also available for this application.

The subcellular localization vectors encode fusions of fluorescent protein variants and localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure. The vectors are available in a variety of organelle- and cytoskeleton-targeted color variants. They are ideal for multiplex labeling experiments.

Photoactivatable mCherry vectors

PAmCherry is a photoactivatable mutant of mCherry that is nonfluorescent until it is exposed to 350–400 nm light. By selecting which cellular regions to activate, you can track organelles against a dark background. PAmCherry subcellular localization vectors are available that target PAmCherry to the cell membrane, mitochondria, actin, or tubulin. Observe photoactivated PAmCherry with the same filter sets used for other red fluorescent proteins, such as DsRed variants and mCherry.

Lenti-X Actin Dynamics Monitoring Kit

This kit is designed to monitor the highly dynamic behavior of the actin filament system in live cells. The kit includes lentiviral vectors encoding actin fusions to DD-AcGFP1 (green, destabilized) and mCherry (red), and the DD's stabilizing ligand Shield1. DD-AcGFP1-Actin is continuously targeted for degradation in the cell by the proteasomes unless the cells are cultured in medium containing the stabilizing ligand, Shield1. By contrast, mCherry-Actin (which does not contain the DD) has normal stability upon expression and is constitutively present in the cell.

Adding and removing Shield1 creates a pulse-chase-like set of conditions which allow you to monitor polymerization of actin monomers as newly synthesized DD-AcGFP1-Actin that is stabilized as Shield1 (green) is integrated into the existing (red) mCherry-Actin actin filament network.

Autophagy sensor vector

pAutophagSENSE is a mammalian expression vector that allows you to monitor the process of autophagy. pAutophagSENSE encodes a fusion of the green fluorescent protein AcGFP1 and the rat LC3 protein. In cells not undergoing autophagy, the AcGFP1-LC3 fusion protein is evenly distributed in the cytoplasm of a transfected cell. However, if a cell is undergoing autophagy via the formation of autophagosomes, the AcGFP1-LC3 fusion protein is incorporated into autophagosomes. This process can be monitored by following the redistribution of the green fluorescent fusion protein from the cytosol to the forming autophagosomes. The readout is a change from an even cytosolic distribution of green fluorescence in cells to a punctate pattern representing the forming autophagosomes. pAutophagSENSE saves you money compared to other autophagy detection systems, because it is vector-based—not a consumable you have to buy over and over again.

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Cat. # Product Size Price License Quantity Details
631076 Lenti-X™ Actin Dynamics Monitoring Kit Each Inquire for Quotation

License Statement

ID Number  
57 This product is covered by U.S. Patent Nos. 8,173,792 and 9,487,787.
39 AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.
*

Use this kit to monitor the highly dynamic behavior of the actin filament system in live cells. The Lenti-X Actin Dynamics Monitoring Kit includes lentiviral vectors encoding actin fusions to DD-AcGFP1 (green, destabilized) and mCherry (red), as well as the DD's stabilizing ligand Shield1. Once your cells have been infected with the included vectors, DD-AcGFP1-Actin is continuously targeted for degradation by the proteasomes, unless the cells are cultured in medium containing the stabilizing ligand Shield1. By contrast, mCherry-Actin (which does not contain the DD) has normal stability and is constitutively present in the cell.

Adding and removing Shield1 creates a "pulse-chase" like set of conditions which allow you to monitor how newly synthesized, Shield-stabilized DD-AcGFP1-Actin (green) is integrated into the existing (red) mCherry-Actin actin filament network.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications. AcGFP1 and DsRed2 protein fusions were transiently transfected and visualized by fluorescence microscopy. Panel A. pAcGFP1-Mito (mitochondria) and pDsRed2-Nuc (nucleus) in HEK 293 cells. Panel B. pAcGFP1-Golgi (Golgi apparatus) and pDsRed2-Nuc (nucleus) in HEK 293 cells.

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Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors.

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Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure. The vectors are available in a variety of organelle- and cytoskeleton-targeted color variants.

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Observe actin filament remodeling using the Lenti-X Actin Dynamics Monitoring Kit

Observe actin filament remodeling using the Lenti-X Actin Dynamics Monitoring Kit

Observe actin filament remodeling using the Lenti-X Actin Dynamics Monitoring Kit. Actin filaments are completely remodeled in HeLa cells in less than 1 hr. Panel A. Self-assembly of actin filaments occurs at the plus end of an existing actin filament as monomeric actin is incorporated. Conversely, disassembly occurs at the minus end where actin monomers depolymerize from the filament, causing a continuous rearrangement of the actin filament network. HeLa cells were infected with constructs encoding mCherry human alpha-Actin and DD-AcGPF1 human alpha-Actin. Cells were fixed using 4% paraformaldehyde and imaged with a 40X objective. Fluorescence micrographs were taken 1 hr after addition of Shield1 (Panels E–G) or without Shield1 (Panels B–D). In the absence of Shield1, DD-AcGFP1-Actin was degraded quickly (Panels C & D) despite a normal, mCherry-labeled actin filament network (Panels B & D). In the presence of Shield1, DD-AcGFP1-Actin was stabilized and present in the actin filament network along with mCherry-labeled Actin (Panels E–G). Our results are in agreement with a previous report that the actin filament network rearranges completely in 1 hr in PtK2 epithelial cells, but we used the extremely simple ProteoTuner-based method, rather than the extremely laborious microinjection method.

631078 pLVX-mCherry-Actin Vector 10 ug Inquire for Quotation *

This lentiviral expression vector encodes human β-actin fused to mCherry, an extremely bright red fluorescent protein. pLVX-mCherry-Actin constitutively expresses the mCherry-Actin fusion protein from PCMV IE when transduced into target cells. The mCherry-Actin fusion protein is incorporated into actin filaments, and allows for visualization of actin-containing subcellular structures in live cells as well as fixed cells. pLVX-mCherry-Actin is not designed to be used as a cloning vector.

To package the vector into high-titer, replication-incompetent lentiviral particles, we recommend using Lenti-X Packaging Single Shots (Cat. No. 632176) and the Lenti-X 293T Cell Line (Cat. No. 632180). The resulting lentivirus can then be used to transduce virtually any mammalian cell type.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications. AcGFP1 and DsRed2 protein fusions were transiently transfected and visualized by fluorescence microscopy. Panel A. pAcGFP1-Mito (mitochondria) and pDsRed2-Nuc (nucleus) in HEK 293 cells. Panel B. pAcGFP1-Golgi (Golgi apparatus) and pDsRed2-Nuc (nucleus) in HEK 293 cells.

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Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors.

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Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure. The vectors are available in a variety of organelle- and cytoskeleton-targeted color variants.

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631078: pLVX-mCherry Actin Vector

631078: pLVX-mCherry Actin Vector
632408 pDsRed2-Nuc Vector 20 ug USD $615.00

pDsRed2-Nuc encodes Discosoma sp. red fluorescent protein (DsRed2) fused with three copies of the nuclear localization signal (NLS) of the SV40 T-antigen. The NLS sequences are fused to the 3'-end of DsRed2. DsRed2 is a human codon-optimized variant of wild-type DsRed that has been engineered for faster maturation and lower non-specific aggregation. pDsRed2-Nuc is designed for fluorescent labeling of the nucleus in living cells.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications. AcGFP1 and DsRed2 protein fusions were transiently transfected and visualized by fluorescence microscopy. Panel A. pAcGFP1-Mito (mitochondria) and pDsRed2-Nuc (nucleus) in HEK 293 cells. Panel B. pAcGFP1-Golgi (Golgi apparatus) and pDsRed2-Nuc (nucleus) in HEK 293 cells.

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Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors.

Back

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure. The vectors are available in a variety of organelle- and cytoskeleton-targeted color variants.

632409 pDsRed2-ER Vector 20 ug USD $615.00

pDsRed2-ER is a mammalian expression vector designed to label the endoplasmic reticulum in living cells. The vector encodes a fusion consisting of Discosoma sp. red fluorescent protein (DsRed2); the endoplasmic reticulum (ER) targeting sequence of calreticulin, fused to the 5' end of DsRed2; and the ER retention sequence, KDEL, fused to the 3' end of DsRed2. DsRed2 is a human codon-optimized variant of wild-type DsRed that has been engineered for faster maturation and lower non-specific aggregation.

pDsRed2-ER can be introduced into mammalian cells using any standard transfection method. If required, stable transformants can be selected using G418.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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632409: pDsRed2-ER Vector

632409: pDsRed2-ER Vector

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AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications. AcGFP1 and DsRed2 protein fusions were transiently transfected and visualized by fluorescence microscopy. Panel A. pAcGFP1-Mito (mitochondria) and pDsRed2-Nuc (nucleus) in HEK 293 cells. Panel B. pAcGFP1-Golgi (Golgi apparatus) and pDsRed2-Nuc (nucleus) in HEK 293 cells.

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Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors.

Back

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure. The vectors are available in a variety of organelle- and cytoskeleton-targeted color variants.

632418 pDsRed2-Peroxi Vector 20 ug USD $615.00

pDsRed2-Peroxi encodes a fusion of Discosoma sp. red fluorescent protein (DsRed2) and the peroxisomal targeting signal 1 (PTS1). The PTS1 sequence is fused to the 3'-end of DsRed2 and encodes the tripeptide SKL, which targets the fusion protein to the matrix of peroxisomes. DsRed2 is human codon-optimized for high expression in mammalian cells. pDsRed2-Peroxi is designed for fluorescent labeling of peroxisomes.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications. AcGFP1 and DsRed2 protein fusions were transiently transfected and visualized by fluorescence microscopy. Panel A. pAcGFP1-Mito (mitochondria) and pDsRed2-Nuc (nucleus) in HEK 293 cells. Panel B. pAcGFP1-Golgi (Golgi apparatus) and pDsRed2-Nuc (nucleus) in HEK 293 cells.

Back

Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors.

Back

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure. The vectors are available in a variety of organelle- and cytoskeleton-targeted color variants.

632419 pDsRed2-Bid Vector 20 ug USD $615.00

pDsRed2-Bid encodes a fusion of Discosoma sp. red fluorescent protein (DsRed2) and Bid, a member of the Bcl-2 family. In healthy, non-apoptotic cells, Bid resides in the cytosol as soluble protein. Upon induction of apoptosis, Bid translocates to mitochondria. In cells expressing the Bid-DsRed2 fusion, the translocation can be detected by fluorescence microscopy.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Monitoring Bid activation with the pDsRed2-Bid Vector

Monitoring Bid activation with the pDsRed2-Bid Vector

Monitoring Bid activation with the pDsRed2-Bid Vector. 3T3 cells were transiently transfected with pDsRed2-Bid (Panels A & B). Approximately 24 hr later, cells were incubated with 700 nM staurosporine, an apoptosis inducing agent, at 37°C for 3 hr. Cells were then fixed and examined with a Zeiss Axioskop equipped with the appropriate light filters. In untreated cells (Panel A), the Bid reporter (Bid-DsRed2) distributes uniformly in the cytosol, as expected. Following induction (Panel B), the reporter translocates to the surface of mitochondria, forming intensely fluorescent clusters—clear evidence that the Bid pathway has been activated.

632421 pDsRed2-Mito Vector 20 ug USD $615.00

pDsRed2-Mito encodes a fusion of Discosoma sp. red fluorescent protein (DsRed2) and a mitochondrial targeting sequence of human cytochrome c oxidase subunit VIII (Mito). The targeting sequence is fused to the 5'-end of DsRed2, which is human codon-optimized for high expression in mammalian cells. pDsRed2-Mito is designed for fluorescent labeling of mitochondria.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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632421: pDsRed2-Mito Vector

632421: pDsRed2-Mito Vector

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AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications. AcGFP1 and DsRed2 protein fusions were transiently transfected and visualized by fluorescence microscopy. Panel A. pAcGFP1-Mito (mitochondria) and pDsRed2-Nuc (nucleus) in HEK 293 cells. Panel B. pAcGFP1-Golgi (Golgi apparatus) and pDsRed2-Nuc (nucleus) in HEK 293 cells.

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Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors.

Back

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure. The vectors are available in a variety of organelle- and cytoskeleton-targeted color variants.

632431 pAcGFP1-Nuc Vector 20 ug Inquire for Quotation

License Statement

ID Number  
72 Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pAcGFP1-Nuc Vector product License Agreement can be found by clicking here.
39 AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.
*

pAcGFP1-Nuc encodes a humanized green fluorescent protein (GFP) derived from Aequorea coerulescens. The fluorescent protein is fused at its C-terminus to three copies of the nuclear localization signal (NLS) of the SV40 T-antigen. This vector is designed a marker for visualizing the nucleus in living or fixed cells by fluorescence microscopy.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Use of AcGFP1 for fusions and fluorescence microscopy applications

Use of AcGFP1 for fusions and fluorescence microscopy applications

Use of AcGFP1 for fusions and fluorescence microscopy applications. Panels A and B. Activation of Protein Kinase C alpha was monitored with Living Colors AcGFP1. Panel A. HEK 293 cells were stably transfected with a plasmid encoding AcGFP1 fused to PKC alpha. Panel B. Cells were induced with 1.5 µg/ml PMA for 3 min. The PKC alpha-AcGFP1 fusion moves from the cytosol to the plasma membrane, a result consistent with the known mobilization pattern of PKC alpha. Panel C. HeLa cells were transiently transfected with pAcGFP1-Actin and visualized by fluorescence microscopy.

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AcGFP1 is a monomeric protein

AcGFP1 is a monomeric protein

AcGFP1 is a monomeric protein. Panel A. Recombinant AcGFP1 protein was analyzed by FPLC gel filtration chromatography. Overall protein absorbance (A280) and chromophore excitation (A477) of the eluted material were monitored simultaneously. AcGFP1 elutes from the column at a retention time corresponding to a molecular weight of 24 kDa. The calculated molecular weight of AcGFP1 is 26.9 kDa. Panel B. Recombinant AcGFP1 protein was analyzed by sucrose density ultracentrifugation using a continuous gradient. Panel C. Pseudonative gel analysis of proteins. The oligomeric structure of proteins is preserved during SDS-PAGE analysis if samples are kept at 4°C and not boiled prior to loading on a gel. Boiled and unboiled recombinant proteins (7.5 μg) were separated by SDS-PAGE electrophoresis (12% acrylamide). In both the boiled (denatured) and unboiled (nondenatured) samples, AcGFP1 runs as a uniform band of ~30 kDa due to its monomeric structure. The unboiled (nondenatured) DsRed-Express runs at a much higher molecular weight than its denatured (boiled) counterpart due to its oligomeric structure.

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AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications. AcGFP1 and DsRed2 protein fusions were transiently transfected and visualized by fluorescence microscopy. Panel A. pAcGFP1-Mito (mitochondria) and pDsRed2-Nuc (nucleus) in HEK 293 cells. Panel B. pAcGFP1-Golgi (Golgi apparatus) and pDsRed2-Nuc (nucleus) in HEK 293 cells.

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Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors.

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Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure. The vectors are available in a variety of organelle- and cytoskeleton-targeted color variants.

632432 pAcGFP1-Mito Vector 20 ug Inquire for Quotation

License Statement

ID Number  
72 Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pAcGFP1-Mito Vector product License Agreement can be found by clicking here.
39 AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.
*

pAcGFP1-Mito encodes a humanized green fluorescent protein (GFP) derived from Aequorea coerulescens. A mitochondrial targeting sequence from the precursor protein of human cytochrome c oxidase subunit VIII is fused to the N-terminus of the fluorescent protein. The fluorescence from pAcGFP1-Mito expression can be observed within the mitochondrial matrix inside the inner membrane. pAcGFP1-Mito can be used for specific labeling of mitochondria in living and fixed cells.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Use of AcGFP1 for fusions and fluorescence microscopy applications

Use of AcGFP1 for fusions and fluorescence microscopy applications

Use of AcGFP1 for fusions and fluorescence microscopy applications. Panels A and B. Activation of Protein Kinase C alpha was monitored with Living Colors AcGFP1. Panel A. HEK 293 cells were stably transfected with a plasmid encoding AcGFP1 fused to PKC alpha. Panel B. Cells were induced with 1.5 µg/ml PMA for 3 min. The PKC alpha-AcGFP1 fusion moves from the cytosol to the plasma membrane, a result consistent with the known mobilization pattern of PKC alpha. Panel C. HeLa cells were transiently transfected with pAcGFP1-Actin and visualized by fluorescence microscopy.

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AcGFP1 is a monomeric protein

AcGFP1 is a monomeric protein

AcGFP1 is a monomeric protein. Panel A. Recombinant AcGFP1 protein was analyzed by FPLC gel filtration chromatography. Overall protein absorbance (A280) and chromophore excitation (A477) of the eluted material were monitored simultaneously. AcGFP1 elutes from the column at a retention time corresponding to a molecular weight of 24 kDa. The calculated molecular weight of AcGFP1 is 26.9 kDa. Panel B. Recombinant AcGFP1 protein was analyzed by sucrose density ultracentrifugation using a continuous gradient. Panel C. Pseudonative gel analysis of proteins. The oligomeric structure of proteins is preserved during SDS-PAGE analysis if samples are kept at 4°C and not boiled prior to loading on a gel. Boiled and unboiled recombinant proteins (7.5 μg) were separated by SDS-PAGE electrophoresis (12% acrylamide). In both the boiled (denatured) and unboiled (nondenatured) samples, AcGFP1 runs as a uniform band of ~30 kDa due to its monomeric structure. The unboiled (nondenatured) DsRed-Express runs at a much higher molecular weight than its denatured (boiled) counterpart due to its oligomeric structure.

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AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications. AcGFP1 and DsRed2 protein fusions were transiently transfected and visualized by fluorescence microscopy. Panel A. pAcGFP1-Mito (mitochondria) and pDsRed2-Nuc (nucleus) in HEK 293 cells. Panel B. pAcGFP1-Golgi (Golgi apparatus) and pDsRed2-Nuc (nucleus) in HEK 293 cells.

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Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors.

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Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure. The vectors are available in a variety of organelle- and cytoskeleton-targeted color variants.

632433 pHcRed1-Nuc Vector 20 ug USD $615.00

pHcRed1-Nuc Vector encodes a far-red fluorescent protein HcRed1 fused with three copies of the nuclear localization signal (NLS) of the SV40 T-antigen. The NLS sequences are fused to the 3'-end of HcRed1. HcRed1 was generated by mutagenesis of a non-fluorescent chromoprotein from the reef coral Heteractis crispa. The coding sequence for HcRed1 is human codon-optimized for higher expression in mammalian cells. pHcRed1-Nuc is designed for visualizing the nucleus in living or fixed cells by fluorescence microscopy.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications. AcGFP1 and DsRed2 protein fusions were transiently transfected and visualized by fluorescence microscopy. Panel A. pAcGFP1-Mito (mitochondria) and pDsRed2-Nuc (nucleus) in HEK 293 cells. Panel B. pAcGFP1-Golgi (Golgi apparatus) and pDsRed2-Nuc (nucleus) in HEK 293 cells.

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Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors.

Back

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure. The vectors are available in a variety of organelle- and cytoskeleton-targeted color variants.

632434 pHcRed1-Mito Vector 20 ug USD $615.00

pHcRed1-Mito encodes a far-red fluorescent protein HcRed1 fused with mitochondrial targeting sequence from the precursor protein of human cytochrome c oxidase subunit VIII. HcRed1 is a fluorescent variant of a chromoprotein found in the reef coral Heteractis crispa. The coding sequence for HcRed1 has been human codon-optimized for higher expression in mammalian cells. pHcRed1-Mito can be used for specific labeling of mitochondria in living cells.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

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AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications. AcGFP1 and DsRed2 protein fusions were transiently transfected and visualized by fluorescence microscopy. Panel A. pAcGFP1-Mito (mitochondria) and pDsRed2-Nuc (nucleus) in HEK 293 cells. Panel B. pAcGFP1-Golgi (Golgi apparatus) and pDsRed2-Nuc (nucleus) in HEK 293 cells.

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Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors.

Back

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure. The vectors are available in a variety of organelle- and cytoskeleton-targeted color variants.

632453 pAcGFP1-Actin Vector 20 ug Inquire for Quotation

License Statement

ID Number  
39 AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.
*

pAcGFP1-Actin encodes a fusion protein consisting of Aequorea coerulescens green fluorescent protein (AcGFP1) fused at its C-terminus to the human cytoplasmic b-actin. The AcGFP1-Actin fusion protein incorporates into growing actin filaments, allowing visualization of the actin cytoskeleton in living or fixed cells.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Living Colors AcGFP1 is ideal for fluorescence microscopy applications

Living Colors AcGFP1 is ideal for fluorescence microscopy applications
Living Colors AcGFP1 is ideal for fluorescence microscopy applications. HeLa cells were transiently transfected with pAcGFP1-Actin (actin) and visualized by fluorescence microscopy.

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632453: pAcGFP1-Actin Vector

632453: pAcGFP1-Actin Vector

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AcGFP1 is a monomeric protein

AcGFP1 is a monomeric protein

AcGFP1 is a monomeric protein. Panel A. Recombinant AcGFP1 protein was analyzed by FPLC gel filtration chromatography. Overall protein absorbance (A280) and chromophore excitation (A477) of the eluted material were monitored simultaneously. AcGFP1 elutes from the column at a retention time corresponding to a molecular weight of 24 kDa. The calculated molecular weight of AcGFP1 is 26.9 kDa. Panel B. Recombinant AcGFP1 protein was analyzed by sucrose density ultracentrifugation using a continuous gradient. Panel C. Pseudonative gel analysis of proteins. The oligomeric structure of proteins is preserved during SDS-PAGE analysis if samples are kept at 4°C and not boiled prior to loading on a gel. Boiled and unboiled recombinant proteins (7.5 μg) were separated by SDS-PAGE electrophoresis (12% acrylamide). In both the boiled (denatured) and unboiled (nondenatured) samples, AcGFP1 runs as a uniform band of ~30 kDa due to its monomeric structure. The unboiled (nondenatured) DsRed-Express runs at a much higher molecular weight than its denatured (boiled) counterpart due to its oligomeric structure.

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AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications. AcGFP1 and DsRed2 protein fusions were transiently transfected and visualized by fluorescence microscopy. Panel A. pAcGFP1-Mito (mitochondria) and pDsRed2-Nuc (nucleus) in HEK 293 cells. Panel B. pAcGFP1-Golgi (Golgi apparatus) and pDsRed2-Nuc (nucleus) in HEK 293 cells.

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Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors.

Back

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure. The vectors are available in a variety of organelle- and cytoskeleton-targeted color variants.

632464 pAcGFP1-Golgi Vector 20 ug Inquire for Quotation

License Statement

ID Number  
72 Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pAcGFP1-Golgi Vector product License Agreement can be found by clicking here.
39 AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.
*

The pAcGFP1-Golgi Vector encodes a fusion protein consisting of Aequorea coerulescens green fluorescent protein (AcGFP1) fused at its N-terminus to 81 amino acids of the precursor to the human beta 1,4-galactosyltransferase (GT). AcGFP1 protein is optimized for brighter fluorescence and higher expression in mammalian cells. pAcGFP1-Golgi can be used for specific labeling of the trans-medial region of the Golgi apparatus in mammalian cells.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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632464: pAcGFP1-Golgi Vector

632464: pAcGFP1-Golgi Vector

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AcGFP1 is a monomeric protein

AcGFP1 is a monomeric protein

AcGFP1 is a monomeric protein. Panel A. Recombinant AcGFP1 protein was analyzed by FPLC gel filtration chromatography. Overall protein absorbance (A280) and chromophore excitation (A477) of the eluted material were monitored simultaneously. AcGFP1 elutes from the column at a retention time corresponding to a molecular weight of 24 kDa. The calculated molecular weight of AcGFP1 is 26.9 kDa. Panel B. Recombinant AcGFP1 protein was analyzed by sucrose density ultracentrifugation using a continuous gradient. Panel C. Pseudonative gel analysis of proteins. The oligomeric structure of proteins is preserved during SDS-PAGE analysis if samples are kept at 4°C and not boiled prior to loading on a gel. Boiled and unboiled recombinant proteins (7.5 μg) were separated by SDS-PAGE electrophoresis (12% acrylamide). In both the boiled (denatured) and unboiled (nondenatured) samples, AcGFP1 runs as a uniform band of ~30 kDa due to its monomeric structure. The unboiled (nondenatured) DsRed-Express runs at a much higher molecular weight than its denatured (boiled) counterpart due to its oligomeric structure.

Back

AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications. AcGFP1 and DsRed2 protein fusions were transiently transfected and visualized by fluorescence microscopy. Panel A. pAcGFP1-Mito (mitochondria) and pDsRed2-Nuc (nucleus) in HEK 293 cells. Panel B. pAcGFP1-Golgi (Golgi apparatus) and pDsRed2-Nuc (nucleus) in HEK 293 cells.

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Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors.

Back

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure. The vectors are available in a variety of organelle- and cytoskeleton-targeted color variants.

632479 pDsRed-Monomer-Actin Vector 20 ug Inquire for Quotation *

pDsRed-Monomer-Actin encodes DsRed-Monomer, a monomeric mutant of the Discosoma sp. red fluorescent protein DsRed. In this vector, the DsRed-Monomer coding sequence is fused at its C-terminus to full-length human cytoplasmic ß-actin. The pDsRed-Monomer-Actin fusion protein incorporates into growing actin filaments, allowing visualization of the actin cytoskeleton in living or fixed cells.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors.

Back

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure. The vectors are available in a variety of organelle- and cytoskeleton-targeted color variants.

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DsRed-Monomer is soluble when expressed in mammalian cells

DsRed-Monomer is soluble when expressed in mammalian cells
DsRed-Monomer is soluble when expressed in mammalian cells. HeLa cells were transfected with pDsRed-Monomer-N1 and fixed in 4% paraformaldehyde 24 hr post-transfection. DsRed-Monomer fluorescent protein displays an even, consistent, and homogeneous distribution.

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DsRed-Monomer is a monomeric protein

DsRed-Monomer is a monomeric protein

DsRed-Monomer is a monomeric protein. Panel A. Recombinant DsRed-Express and DsRed-Monomer fluorescent proteins (100 μg) were analyzed by FPLC gel filtration chromatography. Overall absorbance (A280) and chromophore excitation (A557) of the eluted material were monitored simultaneously. DsRed-Monomer elutes from the column at a retention time (39 min) corresponding to a molecular weight of 28 kDa. The calculated molecular weight of DsRed-Monomer is 26.8 kDa. DsRed-Express is a tetrameric protein that elutes at an earlier retention time (33 min) corresponding to a molecular weight of 89 kDa. Panel B. Pseudonative gel analysis of proteins. The oligomeric structure of proteins is preserved during SDS PAGE analysis if samples are kept at 4°C and not boiled prior to loading on a gel. Boiled and unboiled recombinant proteins (7.5 μg) were separated by SDS PAGE electrophoresis (12% acrylamide). In both the boiled (denatured) and unboiled (nondenatured) samples, DsRed-Monomer fluorescent protein runs as a uniform band of ~30 kDa due to its monomeric structure. The unboiled (nondenatured) DsRed-Express runs at a much higher molecular weight than its boiled (denatured) counterpart due to its tetrameric structure.

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Fluorescence excitation and emission spectra of DsRed-Monomer and AcGFP1

Fluorescence excitation and emission spectra of DsRed-Monomer and AcGFP1
Fluorescence excitation and emission spectra of DsRed-Monomer and AcGFP1.
632480 pDsRed-Monomer-Golgi Vector 20 ug Inquire for Quotation *

pDsRed-Monomer-Golgi encodes DsRed-Monomer, a monomeric mutant of the Discosoma sp. red fluorescent protein DsRed. In this vector, the DsRed-Monomer coding sequence is fused at its N-terminus to the N-terminal 81 amino acids of human beta 1,4-galactosyltransferase (GT). The pDsRed-Monomer-Golgi fusion protein can be used for specific labeling of the trans-medial region of the Golgi apparatus in mammalian cells. A membrane anchoring signal peptide within the 81 amino acid sequence targets the fusion protein.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors.

Back

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure. The vectors are available in a variety of organelle- and cytoskeleton-targeted color variants.

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DsRed-Monomer is soluble when expressed in mammalian cells

DsRed-Monomer is soluble when expressed in mammalian cells
DsRed-Monomer is soluble when expressed in mammalian cells. HeLa cells were transfected with pDsRed-Monomer-N1 and fixed in 4% paraformaldehyde 24 hr post-transfection. DsRed-Monomer fluorescent protein displays an even, consistent, and homogeneous distribution.

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DsRed-Monomer is a monomeric protein

DsRed-Monomer is a monomeric protein

DsRed-Monomer is a monomeric protein. Panel A. Recombinant DsRed-Express and DsRed-Monomer fluorescent proteins (100 μg) were analyzed by FPLC gel filtration chromatography. Overall absorbance (A280) and chromophore excitation (A557) of the eluted material were monitored simultaneously. DsRed-Monomer elutes from the column at a retention time (39 min) corresponding to a molecular weight of 28 kDa. The calculated molecular weight of DsRed-Monomer is 26.8 kDa. DsRed-Express is a tetrameric protein that elutes at an earlier retention time (33 min) corresponding to a molecular weight of 89 kDa. Panel B. Pseudonative gel analysis of proteins. The oligomeric structure of proteins is preserved during SDS PAGE analysis if samples are kept at 4°C and not boiled prior to loading on a gel. Boiled and unboiled recombinant proteins (7.5 μg) were separated by SDS PAGE electrophoresis (12% acrylamide). In both the boiled (denatured) and unboiled (nondenatured) samples, DsRed-Monomer fluorescent protein runs as a uniform band of ~30 kDa due to its monomeric structure. The unboiled (nondenatured) DsRed-Express runs at a much higher molecular weight than its boiled (denatured) counterpart due to its tetrameric structure.

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Fluorescence excitation and emission spectra of DsRed-Monomer and AcGFP1

Fluorescence excitation and emission spectra of DsRed-Monomer and AcGFP1
Fluorescence excitation and emission spectra of DsRed-Monomer and AcGFP1.
632488 pAcGFP1-Tubulin Vector 20 ug Inquire for Quotation

License Statement

ID Number  
72 Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pAcGFP1-Tubulin Vector product License Agreement can be found by clicking here.
39 AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.
*

pAcGFP1-Tubulin encodes a fusion protein consisting of the AcGFP1 green fluorescent protein (GFP) and human a-tubulin. AcGFP1 is an Aequorea coerulescens GFP that has been optimized for brighter fluorescence and higher expression in mammalian cells. The AcGFP1-Tubulin fusion protein incorporates into growing microtubules, allowing visualization of the microtubule network in living or fixed cells.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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AcGFP1 is a monomeric protein

AcGFP1 is a monomeric protein

AcGFP1 is a monomeric protein. Panel A. Recombinant AcGFP1 protein was analyzed by FPLC gel filtration chromatography. Overall protein absorbance (A280) and chromophore excitation (A477) of the eluted material were monitored simultaneously. AcGFP1 elutes from the column at a retention time corresponding to a molecular weight of 24 kDa. The calculated molecular weight of AcGFP1 is 26.9 kDa. Panel B. Recombinant AcGFP1 protein was analyzed by sucrose density ultracentrifugation using a continuous gradient. Panel C. Pseudonative gel analysis of proteins. The oligomeric structure of proteins is preserved during SDS-PAGE analysis if samples are kept at 4°C and not boiled prior to loading on a gel. Boiled and unboiled recombinant proteins (7.5 μg) were separated by SDS-PAGE electrophoresis (12% acrylamide). In both the boiled (denatured) and unboiled (nondenatured) samples, AcGFP1 runs as a uniform band of ~30 kDa due to its monomeric structure. The unboiled (nondenatured) DsRed-Express runs at a much higher molecular weight than its denatured (boiled) counterpart due to its oligomeric structure.

Back

AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications. AcGFP1 and DsRed2 protein fusions were transiently transfected and visualized by fluorescence microscopy. Panel A. pAcGFP1-Mito (mitochondria) and pDsRed2-Nuc (nucleus) in HEK 293 cells. Panel B. pAcGFP1-Golgi (Golgi apparatus) and pDsRed2-Nuc (nucleus) in HEK 293 cells.

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Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors.

Back

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure. The vectors are available in a variety of organelle- and cytoskeleton-targeted color variants.

632490 pAcGFP1-Endo Vector 20 ug Inquire for Quotation

License Statement

ID Number  
72 Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pAcGFP1-Endo Vector product License Agreement can be found by clicking here.
39 AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.
*

pAcGFP1-Endo encodes a fusion protein consisting of the Aequorea coerulescens green fluorescent protein, c-Myc, and RhoB protein. The RhoB GTPase localizes the fusion protein to vesicles involved in endocytosis and allows monitoring of intracellular membrane traffic in living and fixed cells using fluorescence microscopy. The c-Myc epitope allows detection of the fusion protein using c-Myc antibodies. AcGFP1 is a variant of the Aequorea coerulescens green fluorescent protein (AcGFP) that has been optimized for brighter fluorescence and higher expression in mammalian cells.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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632490: pAcGFP1-Endo Vector

632490: pAcGFP1-Endo Vector

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AcGFP1 is a monomeric protein

AcGFP1 is a monomeric protein

AcGFP1 is a monomeric protein. Panel A. Recombinant AcGFP1 protein was analyzed by FPLC gel filtration chromatography. Overall protein absorbance (A280) and chromophore excitation (A477) of the eluted material were monitored simultaneously. AcGFP1 elutes from the column at a retention time corresponding to a molecular weight of 24 kDa. The calculated molecular weight of AcGFP1 is 26.9 kDa. Panel B. Recombinant AcGFP1 protein was analyzed by sucrose density ultracentrifugation using a continuous gradient. Panel C. Pseudonative gel analysis of proteins. The oligomeric structure of proteins is preserved during SDS-PAGE analysis if samples are kept at 4°C and not boiled prior to loading on a gel. Boiled and unboiled recombinant proteins (7.5 μg) were separated by SDS-PAGE electrophoresis (12% acrylamide). In both the boiled (denatured) and unboiled (nondenatured) samples, AcGFP1 runs as a uniform band of ~30 kDa due to its monomeric structure. The unboiled (nondenatured) DsRed-Express runs at a much higher molecular weight than its denatured (boiled) counterpart due to its oligomeric structure.

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AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications. AcGFP1 and DsRed2 protein fusions were transiently transfected and visualized by fluorescence microscopy. Panel A. pAcGFP1-Mito (mitochondria) and pDsRed2-Nuc (nucleus) in HEK 293 cells. Panel B. pAcGFP1-Golgi (Golgi apparatus) and pDsRed2-Nuc (nucleus) in HEK 293 cells.

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Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors.

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Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure. The vectors are available in a variety of organelle- and cytoskeleton-targeted color variants.

632491 pAcGFP1-Mem Vector 20 ug Inquire for Quotation

License Statement

ID Number  
72 Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pAcGFP1-Mem Vector product License Agreement can be found by clicking here.
39 AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.
*

pAcGFP1-Mem encodes a fusion protein consisting of the Aequorea coerulescens green fluorescent protein and the N-terminal membrane-targeting signal of neuromodulin (also called GAP-43). AcGFP1 is a variant of the Aequorea coerulescens green fluorescent protein (AcGFP) that has been optimized for brighter fluorescence and higher expression in mammalian cells. pAcGFP1-Mem can be used for labeling the plasma membrane.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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AcGFP1 is a monomeric protein

AcGFP1 is a monomeric protein

AcGFP1 is a monomeric protein. Panel A. Recombinant AcGFP1 protein was analyzed by FPLC gel filtration chromatography. Overall protein absorbance (A280) and chromophore excitation (A477) of the eluted material were monitored simultaneously. AcGFP1 elutes from the column at a retention time corresponding to a molecular weight of 24 kDa. The calculated molecular weight of AcGFP1 is 26.9 kDa. Panel B. Recombinant AcGFP1 protein was analyzed by sucrose density ultracentrifugation using a continuous gradient. Panel C. Pseudonative gel analysis of proteins. The oligomeric structure of proteins is preserved during SDS-PAGE analysis if samples are kept at 4°C and not boiled prior to loading on a gel. Boiled and unboiled recombinant proteins (7.5 μg) were separated by SDS-PAGE electrophoresis (12% acrylamide). In both the boiled (denatured) and unboiled (nondenatured) samples, AcGFP1 runs as a uniform band of ~30 kDa due to its monomeric structure. The unboiled (nondenatured) DsRed-Express runs at a much higher molecular weight than its denatured (boiled) counterpart due to its oligomeric structure.

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AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications. AcGFP1 and DsRed2 protein fusions were transiently transfected and visualized by fluorescence microscopy. Panel A. pAcGFP1-Mito (mitochondria) and pDsRed2-Nuc (nucleus) in HEK 293 cells. Panel B. pAcGFP1-Golgi (Golgi apparatus) and pDsRed2-Nuc (nucleus) in HEK 293 cells.

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Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors.

Back

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure. The vectors are available in a variety of organelle- and cytoskeleton-targeted color variants.

632493 pDsRed-Monomer-F Vector 20 ug Inquire for Quotation *

pDsRed-Monomer-F encodes a fusion protein consisting of the DsRed-Monomer red fluorescent protein and the farnesylation signal of c-Ha-ras. DsRed-Monomer is a monomeric mutant of the Discosoma sp. red fluorescent protein DsRed. The DsRed-Monomer coding sequence in this construct has been human codon-optimized for efficient expression and enhanced brightness in mammalian cells. pDsRed-Monomer-F can be used for labeling the inner face of the plasma membrane.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications. AcGFP1 and DsRed2 protein fusions were transiently transfected and visualized by fluorescence microscopy. Panel A. pAcGFP1-Mito (mitochondria) and pDsRed2-Nuc (nucleus) in HEK 293 cells. Panel B. pAcGFP1-Golgi (Golgi apparatus) and pDsRed2-Nuc (nucleus) in HEK 293 cells.

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Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors.

Back

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure. The vectors are available in a variety of organelle- and cytoskeleton-targeted color variants.

Back

DsRed-Monomer is soluble when expressed in mammalian cells

DsRed-Monomer is soluble when expressed in mammalian cells
DsRed-Monomer is soluble when expressed in mammalian cells. HeLa cells were transfected with pDsRed-Monomer-N1 and fixed in 4% paraformaldehyde 24 hr post-transfection. DsRed-Monomer fluorescent protein displays an even, consistent, and homogeneous distribution.

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DsRed-Monomer is a monomeric protein

DsRed-Monomer is a monomeric protein

DsRed-Monomer is a monomeric protein. Panel A. Recombinant DsRed-Express and DsRed-Monomer fluorescent proteins (100 μg) were analyzed by FPLC gel filtration chromatography. Overall absorbance (A280) and chromophore excitation (A557) of the eluted material were monitored simultaneously. DsRed-Monomer elutes from the column at a retention time (39 min) corresponding to a molecular weight of 28 kDa. The calculated molecular weight of DsRed-Monomer is 26.8 kDa. DsRed-Express is a tetrameric protein that elutes at an earlier retention time (33 min) corresponding to a molecular weight of 89 kDa. Panel B. Pseudonative gel analysis of proteins. The oligomeric structure of proteins is preserved during SDS PAGE analysis if samples are kept at 4°C and not boiled prior to loading on a gel. Boiled and unboiled recombinant proteins (7.5 μg) were separated by SDS PAGE electrophoresis (12% acrylamide). In both the boiled (denatured) and unboiled (nondenatured) samples, DsRed-Monomer fluorescent protein runs as a uniform band of ~30 kDa due to its monomeric structure. The unboiled (nondenatured) DsRed-Express runs at a much higher molecular weight than its boiled (denatured) counterpart due to its tetrameric structure.

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Fluorescence excitation and emission spectra of DsRed-Monomer and AcGFP1

Fluorescence excitation and emission spectra of DsRed-Monomer and AcGFP1
Fluorescence excitation and emission spectra of DsRed-Monomer and AcGFP1.
632509 pAcGFP1-Mem Hyg Vector 20 ug Inquire for Quotation

License Statement

ID Number  
39 AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.
*

pAcGFP1-Mem Hyg encodes a fusion protein consisting of the Aequorea coerulescens green fluorescent protein and the N-terminal membrane-targeting signal of neuromodulin (also called GAP-43). AcGFP1 is a variant of the Aequorea coerulescens green fluorescent protein (AcGFP) that has been optimized for brighter fluorescence and higher expression in mammalian cells. pAcGFP1-Mem Hyg can be used for labeling the plasma membrane. The hygromycin resistance cassette allows you to create double-stable cell lines if this vector is used in conjunction with any other Living Colors vector that contains a neomycin resistance cassette.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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AcGFP1 is a monomeric protein

AcGFP1 is a monomeric protein

AcGFP1 is a monomeric protein. Panel A. Recombinant AcGFP1 protein was analyzed by FPLC gel filtration chromatography. Overall protein absorbance (A280) and chromophore excitation (A477) of the eluted material were monitored simultaneously. AcGFP1 elutes from the column at a retention time corresponding to a molecular weight of 24 kDa. The calculated molecular weight of AcGFP1 is 26.9 kDa. Panel B. Recombinant AcGFP1 protein was analyzed by sucrose density ultracentrifugation using a continuous gradient. Panel C. Pseudonative gel analysis of proteins. The oligomeric structure of proteins is preserved during SDS-PAGE analysis if samples are kept at 4°C and not boiled prior to loading on a gel. Boiled and unboiled recombinant proteins (7.5 μg) were separated by SDS-PAGE electrophoresis (12% acrylamide). In both the boiled (denatured) and unboiled (nondenatured) samples, AcGFP1 runs as a uniform band of ~30 kDa due to its monomeric structure. The unboiled (nondenatured) DsRed-Express runs at a much higher molecular weight than its denatured (boiled) counterpart due to its oligomeric structure.

Back

AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications. AcGFP1 and DsRed2 protein fusions were transiently transfected and visualized by fluorescence microscopy. Panel A. pAcGFP1-Mito (mitochondria) and pDsRed2-Nuc (nucleus) in HEK 293 cells. Panel B. pAcGFP1-Golgi (Golgi apparatus) and pDsRed2-Nuc (nucleus) in HEK 293 cells.

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Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors.

Back

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure. The vectors are available in a variety of organelle- and cytoskeleton-targeted color variants.

632510 pAcGFP1-F Hyg Vector 20 ug Inquire for Quotation

License Statement

ID Number  
39 AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.
*

pAcGFP1-F encodes a fusion protein consisting of the Aequorea coerulescens green fluorescent protein and the farnesylation signal of c-Ha-ras. AcGFP1 is a variant of the Aequorea coerulescens green fluorescent protein (AcGFP). The AcGFP1 coding sequence in this construct has been optimized for efficient expression and enhanced brightness in mammalian cells. pAcGFP1-F Hyg can be used for labeling the inner leaflet of the plasma membrane. The hygromycin resistance cassette allows you to create double-stable cell lines if this vector is used in conjunction with any other Living Colors vector that contains a neomycin resistance cassette.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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AcGFP1 is a monomeric protein

AcGFP1 is a monomeric protein

AcGFP1 is a monomeric protein. Panel A. Recombinant AcGFP1 protein was analyzed by FPLC gel filtration chromatography. Overall protein absorbance (A280) and chromophore excitation (A477) of the eluted material were monitored simultaneously. AcGFP1 elutes from the column at a retention time corresponding to a molecular weight of 24 kDa. The calculated molecular weight of AcGFP1 is 26.9 kDa. Panel B. Recombinant AcGFP1 protein was analyzed by sucrose density ultracentrifugation using a continuous gradient. Panel C. Pseudonative gel analysis of proteins. The oligomeric structure of proteins is preserved during SDS-PAGE analysis if samples are kept at 4°C and not boiled prior to loading on a gel. Boiled and unboiled recombinant proteins (7.5 μg) were separated by SDS-PAGE electrophoresis (12% acrylamide). In both the boiled (denatured) and unboiled (nondenatured) samples, AcGFP1 runs as a uniform band of ~30 kDa due to its monomeric structure. The unboiled (nondenatured) DsRed-Express runs at a much higher molecular weight than its denatured (boiled) counterpart due to its oligomeric structure.

Back

AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications. AcGFP1 and DsRed2 protein fusions were transiently transfected and visualized by fluorescence microscopy. Panel A. pAcGFP1-Mito (mitochondria) and pDsRed2-Nuc (nucleus) in HEK 293 cells. Panel B. pAcGFP1-Golgi (Golgi apparatus) and pDsRed2-Nuc (nucleus) in HEK 293 cells.

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Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors.

Back

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure. The vectors are available in a variety of organelle- and cytoskeleton-targeted color variants.

632511 pAcGFP1-F Vector 20 ug Inquire for Quotation

License Statement

ID Number  
39 AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.
*

pAcGFP1-F encodes a fusion protein consisting of the Aequorea coerulescens green fluorescent protein and the farnesylation signal of c-Ha-ras. AcGFP1 is a variant of the Aequorea coerulescens green fluorescent protein (AcGFP). The AcGFP1 coding sequence in this construct has been optimized for efficient expression and enhanced brightness in mammalian cells. pAcGFP1-F can be used for labeling the inner leaflet of the plasma membrane.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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632511: pAcGFP1-F Vector

632511: pAcGFP1-F Vector

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AcGFP1 is a monomeric protein

AcGFP1 is a monomeric protein

AcGFP1 is a monomeric protein. Panel A. Recombinant AcGFP1 protein was analyzed by FPLC gel filtration chromatography. Overall protein absorbance (A280) and chromophore excitation (A477) of the eluted material were monitored simultaneously. AcGFP1 elutes from the column at a retention time corresponding to a molecular weight of 24 kDa. The calculated molecular weight of AcGFP1 is 26.9 kDa. Panel B. Recombinant AcGFP1 protein was analyzed by sucrose density ultracentrifugation using a continuous gradient. Panel C. Pseudonative gel analysis of proteins. The oligomeric structure of proteins is preserved during SDS-PAGE analysis if samples are kept at 4°C and not boiled prior to loading on a gel. Boiled and unboiled recombinant proteins (7.5 μg) were separated by SDS-PAGE electrophoresis (12% acrylamide). In both the boiled (denatured) and unboiled (nondenatured) samples, AcGFP1 runs as a uniform band of ~30 kDa due to its monomeric structure. The unboiled (nondenatured) DsRed-Express runs at a much higher molecular weight than its denatured (boiled) counterpart due to its oligomeric structure.

Back

AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications. AcGFP1 and DsRed2 protein fusions were transiently transfected and visualized by fluorescence microscopy. Panel A. pAcGFP1-Mito (mitochondria) and pDsRed2-Nuc (nucleus) in HEK 293 cells. Panel B. pAcGFP1-Golgi (Golgi apparatus) and pDsRed2-Nuc (nucleus) in HEK 293 cells.

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Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors.

Back

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure. The vectors are available in a variety of organelle- and cytoskeleton-targeted color variants.

632512 pDsRed-Monomer-Mem Vector 20 ug Inquire for Quotation *

pDsRed-Monomer-Mem encodes a fusion protein consisting of the DsRed-Monomer red fluorescent protein and the N-terminal membrane-targeting signal of neuromodulin (also called GAP-43). DsRed-Monomer is a monomeric mutant of the Discosoma sp. red fluorescent protein DsRed that has been optimized for efficient expression and enhanced brightness in mammalian cells. pDsRed-Monomer-Mem can be used for labeling the plasma membrane.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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DsRed-Monomer is soluble when expressed in mammalian cells

DsRed-Monomer is soluble when expressed in mammalian cells
DsRed-Monomer is soluble when expressed in mammalian cells. HeLa cells were transfected with pDsRed-Monomer-N1 and fixed in 4% paraformaldehyde 24 hr post-transfection. DsRed-Monomer fluorescent protein displays an even, consistent, and homogeneous distribution.

Back

Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors.

Back

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure. The vectors are available in a variety of organelle- and cytoskeleton-targeted color variants.

Back

DsRed-Monomer is a monomeric protein

DsRed-Monomer is a monomeric protein

DsRed-Monomer is a monomeric protein. Panel A. Recombinant DsRed-Express and DsRed-Monomer fluorescent proteins (100 μg) were analyzed by FPLC gel filtration chromatography. Overall absorbance (A280) and chromophore excitation (A557) of the eluted material were monitored simultaneously. DsRed-Monomer elutes from the column at a retention time (39 min) corresponding to a molecular weight of 28 kDa. The calculated molecular weight of DsRed-Monomer is 26.8 kDa. DsRed-Express is a tetrameric protein that elutes at an earlier retention time (33 min) corresponding to a molecular weight of 89 kDa. Panel B. Pseudonative gel analysis of proteins. The oligomeric structure of proteins is preserved during SDS PAGE analysis if samples are kept at 4°C and not boiled prior to loading on a gel. Boiled and unboiled recombinant proteins (7.5 μg) were separated by SDS PAGE electrophoresis (12% acrylamide). In both the boiled (denatured) and unboiled (nondenatured) samples, DsRed-Monomer fluorescent protein runs as a uniform band of ~30 kDa due to its monomeric structure. The unboiled (nondenatured) DsRed-Express runs at a much higher molecular weight than its boiled (denatured) counterpart due to its tetrameric structure.

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Fluorescence excitation and emission spectra of DsRed-Monomer and AcGFP1

Fluorescence excitation and emission spectra of DsRed-Monomer and AcGFP1
Fluorescence excitation and emission spectra of DsRed-Monomer and AcGFP1.
632513 pDsRed-Monomer-Mem Hyg Vector 20 ug Inquire for Quotation *

pDsRed-Monomer-Mem Hyg encodes a fusion protein consisting of the DsRed-Monomer red fluorescent protein and the N-terminal membrane-targeting signal of neuromodulin (also called GAP-43). DsRed-Monomer is a monomeric mutant of the Discosoma sp. red fluorescent protein DsRed that has been optimized for efficient expression and enhanced brightness in mammalian cells. pDsRed-Monomer-Mem Hyg can be used for labeling the plasma membrane. The hygromycin resistance cassette allows you to create double-stable cell lines if this vector is used in conjunction with any other Living Colors vector that contains a neomycin resistance cassette.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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DsRed-Monomer is soluble when expressed in mammalian cells

DsRed-Monomer is soluble when expressed in mammalian cells
DsRed-Monomer is soluble when expressed in mammalian cells. HeLa cells were transfected with pDsRed-Monomer-N1 and fixed in 4% paraformaldehyde 24 hr post-transfection. DsRed-Monomer fluorescent protein displays an even, consistent, and homogeneous distribution.

Back

Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors.

Back

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure. The vectors are available in a variety of organelle- and cytoskeleton-targeted color variants.

Back

DsRed-Monomer is a monomeric protein

DsRed-Monomer is a monomeric protein

DsRed-Monomer is a monomeric protein. Panel A. Recombinant DsRed-Express and DsRed-Monomer fluorescent proteins (100 μg) were analyzed by FPLC gel filtration chromatography. Overall absorbance (A280) and chromophore excitation (A557) of the eluted material were monitored simultaneously. DsRed-Monomer elutes from the column at a retention time (39 min) corresponding to a molecular weight of 28 kDa. The calculated molecular weight of DsRed-Monomer is 26.8 kDa. DsRed-Express is a tetrameric protein that elutes at an earlier retention time (33 min) corresponding to a molecular weight of 89 kDa. Panel B. Pseudonative gel analysis of proteins. The oligomeric structure of proteins is preserved during SDS PAGE analysis if samples are kept at 4°C and not boiled prior to loading on a gel. Boiled and unboiled recombinant proteins (7.5 μg) were separated by SDS PAGE electrophoresis (12% acrylamide). In both the boiled (denatured) and unboiled (nondenatured) samples, DsRed-Monomer fluorescent protein runs as a uniform band of ~30 kDa due to its monomeric structure. The unboiled (nondenatured) DsRed-Express runs at a much higher molecular weight than its boiled (denatured) counterpart due to its tetrameric structure.

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Fluorescence excitation and emission spectra of DsRed-Monomer and AcGFP1

Fluorescence excitation and emission spectra of DsRed-Monomer and AcGFP1
Fluorescence excitation and emission spectra of DsRed-Monomer and AcGFP1.
632514 pDsRed-Monomer-F Hyg Vector 20 ug Inquire for Quotation *

pDsRed-Monomer-F Hyg encodes a fusion protein consisting of the DsRed-Monomer red fluorescent protein and the farnesylation signal of c-Ha-ras. DsRed-Monomer is a monomeric mutant of the Discosoma sp. red fluorescent protein DsRed. The DsRed-Monomer coding sequence in this construct has been optimized for efficient expression and enhanced brightness in mammalian cells. pDsRed-Monomer-F Hyg can be used for labeling the inner leaflet of the plasma membrane. The hygromycin resistance cassette allows you to create double-stable cell lines if this vector is used in conjunction with any other Living Colors vector that contains a neomycin resistance cassette.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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DsRed-Monomer is soluble when expressed in mammalian cells

DsRed-Monomer is soluble when expressed in mammalian cells
DsRed-Monomer is soluble when expressed in mammalian cells. HeLa cells were transfected with pDsRed-Monomer-N1 and fixed in 4% paraformaldehyde 24 hr post-transfection. DsRed-Monomer fluorescent protein displays an even, consistent, and homogeneous distribution.

Back

AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications. AcGFP1 and DsRed2 protein fusions were transiently transfected and visualized by fluorescence microscopy. Panel A. pAcGFP1-Mito (mitochondria) and pDsRed2-Nuc (nucleus) in HEK 293 cells. Panel B. pAcGFP1-Golgi (Golgi apparatus) and pDsRed2-Nuc (nucleus) in HEK 293 cells.

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Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors.

Back

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure. The vectors are available in a variety of organelle- and cytoskeleton-targeted color variants.

Back

DsRed-Monomer is a monomeric protein

DsRed-Monomer is a monomeric protein

DsRed-Monomer is a monomeric protein. Panel A. Recombinant DsRed-Express and DsRed-Monomer fluorescent proteins (100 μg) were analyzed by FPLC gel filtration chromatography. Overall absorbance (A280) and chromophore excitation (A557) of the eluted material were monitored simultaneously. DsRed-Monomer elutes from the column at a retention time (39 min) corresponding to a molecular weight of 28 kDa. The calculated molecular weight of DsRed-Monomer is 26.8 kDa. DsRed-Express is a tetrameric protein that elutes at an earlier retention time (33 min) corresponding to a molecular weight of 89 kDa. Panel B. Pseudonative gel analysis of proteins. The oligomeric structure of proteins is preserved during SDS PAGE analysis if samples are kept at 4°C and not boiled prior to loading on a gel. Boiled and unboiled recombinant proteins (7.5 μg) were separated by SDS PAGE electrophoresis (12% acrylamide). In both the boiled (denatured) and unboiled (nondenatured) samples, DsRed-Monomer fluorescent protein runs as a uniform band of ~30 kDa due to its monomeric structure. The unboiled (nondenatured) DsRed-Express runs at a much higher molecular weight than its boiled (denatured) counterpart due to its tetrameric structure.

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Fluorescence excitation and emission spectra of DsRed-Monomer and AcGFP1

Fluorescence excitation and emission spectra of DsRed-Monomer and AcGFP1
Fluorescence excitation and emission spectra of DsRed-Monomer and AcGFP1.
632515 pIRES2-AcGFP1-Nuc Vector 20 ug Inquire for Quotation

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72 Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pIRES2-AcGFP1-Nuc Vector product License Agreement can be found by clicking here.
39 AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.
*

pIRES2-AcGFP1-Nuc encodes AcGFP1 with three identical copies of the nuclear localization signal (NLS) of the simian virus 40 large T antigen fused in tandem to its C-terminus. The gene encoding the AcGFP1-Nuc fusion protein is located downstream of the IRES2 sequence. This permits both the gene of interest (cloned into the MCS upstream of the IRES2 sequence) and the AcGFP1-Nuc gene to be translated from a single bicistronic mRNA. AcGFP-1-Nuc localizes to the nucleus of a transfected cell, offering an advantage over random localization in the cytosol. It is most useful for those who are interested in monitoring translocation events in and out of the nucleus.

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Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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AcGFP1 is a monomeric protein

AcGFP1 is a monomeric protein

AcGFP1 is a monomeric protein. Panel A. Recombinant AcGFP1 protein was analyzed by FPLC gel filtration chromatography. Overall protein absorbance (A280) and chromophore excitation (A477) of the eluted material were monitored simultaneously. AcGFP1 elutes from the column at a retention time corresponding to a molecular weight of 24 kDa. The calculated molecular weight of AcGFP1 is 26.9 kDa. Panel B. Recombinant AcGFP1 protein was analyzed by sucrose density ultracentrifugation using a continuous gradient. Panel C. Pseudonative gel analysis of proteins. The oligomeric structure of proteins is preserved during SDS-PAGE analysis if samples are kept at 4°C and not boiled prior to loading on a gel. Boiled and unboiled recombinant proteins (7.5 μg) were separated by SDS-PAGE electrophoresis (12% acrylamide). In both the boiled (denatured) and unboiled (nondenatured) samples, AcGFP1 runs as a uniform band of ~30 kDa due to its monomeric structure. The unboiled (nondenatured) DsRed-Express runs at a much higher molecular weight than its denatured (boiled) counterpart due to its oligomeric structure.

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632515: pIRES2-AcGFP1-Nuc Vector

632515: pIRES2-AcGFP1-Nuc Vector

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Map of the fluorescent pIRES2 bicistronic expression vectors

Map of the fluorescent pIRES2 bicistronic expression vectors
Map of the fluorescent pIRES2 bicistronic expression vectors.

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Expression of two proteins from a single mRNA transcript

Expression of two proteins from a single mRNA transcript
Expression of two proteins from a single mRNA transcript. A fluorescent protein is translated from an internal ribosome entry site (IRES).
632583 pAutophagSENSE Vector Each Inquire for Quotation

License Statement

ID Number  
39 AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.
*

The pAutophagSENSE vector expresses the mutant green fluorescent protein AcGFP1 (excitation maximum = 475 nm; emission maximum = 505 nm) fused to the mouse LC3 protein in mammalian cells. The fusion protein is incorporated into the autophagosomes and is used to monitor autophagosome formation in live cells. This process is monitored by following the redistribution of a green fluorescent fusion protein from the cytosol to the forming autophagosomes.

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Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Live cell monitoring of autophagosome formation

Live cell monitoring of autophagosome formation
Live cell monitoring of autophagosome formation.
632588 pPAmCherry-Mem Vector 10 ug Inquire for Quotation *

pPAmCherry-Mem Vector is mammalian expression vector encoding a fusion protein of PAmCherry and the N-terminal 20 amino acids of neuromodulin (GAP-43). PAmCherry is a photoactivatable mutant of the fluorescent protein mCherry. PAmCherry is non-fluorescence until photoactivated by a short exposure to light at a wavelength between 350 nm and 400 nm. The excitation/emission wavelengths of photoactivated PAmCherry are 564 nm and 595 nm. The GAP-43 fragment contains a signal for posttranslational palmitoylation of cysteins 3 and 4 that targets the fusion protein to the plasma membrane.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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632588: pPAmCherry-Mem Vector

632588: pPAmCherry-Mem Vector

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The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells

The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells

The mCherry monoclonal antibody detects PAmCherry-N1 in the lysate of mammalian cells. HEK 293 cells were transiently transfected with pPAmCherry-N1. Cell lysates (corresponding to 30,000 cells) were prepared from HEK 293 cells transiently expressing PAmCherry-N1 (Lane 1) or a negative control (untransfected cells; Lane 2). Both lysates and a positive control (5 ng recombinant mCherry; Lane 3) were separated by SDS-PAGE and analyzed by Western blot using the mCherry monoclonal antibody at the recommended dilution of 1:1000.

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PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background

PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background
PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background. Visualize PAmCherry with the same filter sets you use to detect other red fluorescent proteins, such as DsRed and mCherry.
632589 pPAmCherry-Actin Vector 10 ug Inquire for Quotation *

pPAmCherry-Actin Vector is a mammalian expression vector encoding PAmCherry, a photoactivatable mutant of the fluorescent protein mCherry, fused to human cytoplasmic beta-actin. PAmCherry is non-fluorescence until photoactivated by a short exposure to light at a wavelength between 350 nm and 400 nm. The excitation/emission wavelengths of photoactivated PAmCherry are 564 nm and 595 nm.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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632589: pPAmCherry-Actin Vector

632589: pPAmCherry-Actin Vector

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The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells

The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells

The mCherry monoclonal antibody detects PAmCherry-N1 in the lysate of mammalian cells. HEK 293 cells were transiently transfected with pPAmCherry-N1. Cell lysates (corresponding to 30,000 cells) were prepared from HEK 293 cells transiently expressing PAmCherry-N1 (Lane 1) or a negative control (untransfected cells; Lane 2). Both lysates and a positive control (5 ng recombinant mCherry; Lane 3) were separated by SDS-PAGE and analyzed by Western blot using the mCherry monoclonal antibody at the recommended dilution of 1:1000.

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PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background

PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background
PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background. Visualize PAmCherry with the same filter sets you use to detect other red fluorescent proteins, such as DsRed and mCherry.
632590 pPAmCherry-Tubulin Vector 10 ug Inquire for Quotation *

pPAmCherry-Tubulin Vector is a mammalian expression vector encoding PAmCherry, a photoactivatable mutant of the fluorescent protein mCherry, fused to human alpha-tubulin. PAmCherry is non-fluorescence until photoactivated by a short exposure to light at a wavelength between 350 nm and 400 nm. The excitation/emission wavelengths of photoactivated PAmCherry are 564 nm and 595 nm.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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632590: pPAmCherry-Tubulin Vector

632590: pPAmCherry-Tubulin Vector

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The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells

The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells

The mCherry monoclonal antibody detects PAmCherry-N1 in the lysate of mammalian cells. HEK 293 cells were transiently transfected with pPAmCherry-N1. Cell lysates (corresponding to 30,000 cells) were prepared from HEK 293 cells transiently expressing PAmCherry-N1 (Lane 1) or a negative control (untransfected cells; Lane 2). Both lysates and a positive control (5 ng recombinant mCherry; Lane 3) were separated by SDS-PAGE and analyzed by Western blot using the mCherry monoclonal antibody at the recommended dilution of 1:1000.

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PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background

PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background
PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background. Visualize PAmCherry with the same filter sets you use to detect other red fluorescent proteins, such as DsRed and mCherry.
632591 pPAmCherry-Mito Vector 10 ug Inquire for Quotation *

pPAmCherry-Mito Vector is a mammalian expression vector encoding PAmCherry, a photoactivatable mutant of the fluorescent protein mCherry, fused to the mitochondrial targeting sequence derived from the precursor subunit VIII of human cytochrome C oxidase. PAmCherry is non-fluorescence until photoactivated by a short exposure to light at a wavelength between 350 nm and 400 nm. The excitation/emission wavelengths of photoactivated PAmCherry are 564 nm and 595 nm.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Photoactivated PAmCherry-Mito shows strong red fluorescence and localizes correctly to the mitochondria

Photoactivated PAmCherry-Mito shows strong red fluorescence and localizes correctly to the mitochondria

Photoactivated PAmCherry-Mito shows strong red fluorescence and localizes correctly to the mitochondria. U2OS cells were transiently transfected with pPAmCherry-Mito. PAmCherry-Mito was activated using an Argon 458 nm laser (scan speed: 400 Hz), and the cells were imaged using a HeNe 543 nm laser for excitation and a standard red fluorescence emission filter set.

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The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells

The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells

The mCherry monoclonal antibody detects PAmCherry-N1 in the lysate of mammalian cells. HEK 293 cells were transiently transfected with pPAmCherry-N1. Cell lysates (corresponding to 30,000 cells) were prepared from HEK 293 cells transiently expressing PAmCherry-N1 (Lane 1) or a negative control (untransfected cells; Lane 2). Both lysates and a positive control (5 ng recombinant mCherry; Lane 3) were separated by SDS-PAGE and analyzed by Western blot using the mCherry monoclonal antibody at the recommended dilution of 1:1000.

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Prior to photoactivation, no red fluorescence is detected in a cell expressing PAmCherry-Mito (Panel A)

Prior to photoactivation, no red fluorescence is detected in a cell expressing PAmCherry-Mito (Panel A)
Prior to photoactivation, no red fluorescence is detected in a cell expressing PAmCherry-Mito (Panel A). However, after photoactivation, strong red fluorescence is observed in the subcellular, activated region of the cell (Panel B). U2OS cells were transiently transfected with pPAmCherry-Mito. Cells were imaged prior to activation in order to determine the level of background fluorescence (Panel A). PAmCherry-Mito was then activated in a small region of a cell imaged using a HeNe 543 nm laser for excitation and a standard red fluorescence emission filter set (Panel B).

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PAmCherry-Mito makes it easy to follow the behavior of a subset of mitochondria

PAmCherry-Mito makes it easy to follow the behavior of a subset of mitochondria

PAmCherry-Mito makes it easy to follow the behavior of a subset of mitochondria. Activating the mitochondria in just one region of the cell makes it possible to follow their movements into dark (nonactivated) areas of the cell. U2OS cells were transiently transfected with pPAmCherry-Mito. PAmCherry-Mito was activated in a small region of a cell, and the cells were imaged every 10 sec for 15 min using a HeNe 543 nm laser for excitation and a standard red fluorescence emission filter set.

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PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background

PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background
PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background. Visualize PAmCherry with the same filter sets you use to detect other red fluorescent proteins, such as DsRed and mCherry.

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632591: pPAmCherry-Mito Vector

632591: pPAmCherry-Mito Vector

*You must be logged in to a Purchasing Account in order to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@takarabio.com.

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Overview

  • Localize fluorescence to specific organelles or structures in living cells
  • Visualize biological processes as they occur
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Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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