Protein transfection reagents

The Xfect Protein Transfection Reagent uses a cell-penetrating peptide to bind and transport active proteins directly into a wide variety of mammalian cell types, including hard-to-transfect human suspension cell lines and mouse embryonic stem cells.

What is the Xfect Protein Transfection Reagent and how does it work?

Xfect Protein Transfection Reagent is a modified peptide with cell-penetrating activity whose amino acid composition enables it to interact with a protein cargo and transport this protein across a cell membrane barrier. Just incubate your protein of interest with the Xfect reagent in the supplied buffer for 30 minutes and then apply the mixture to your cells. Two hours later you are ready to assay for protein activity.

Why transfect purified proteins directly?

Protein transfection is extremely rapid compared to traditional gene expression studies using transfected DNA (1–2 hours compared to 18–48 hours) because it bypasses cellular processes such as transcription and translation. It also facilitates studies involving transient effects of proteins, and avoids potentially harmful random DNA integration into the genome of the target cells. Xfect Protein Transfection Reagent makes it possible to deliver active proteins directly into cells for studies that involve transcriptional regulation, the cell cycle, apoptosis, oncogenesis, epigenetics, cell regeneration, and transdifferentiation.

What are the advantages of Xfect Transfection Reagent compared to other protein delivery technologies?

The two technologies most frequently used for protein delivery are based on lipids and cell-penetrating peptides. Lipid-based reagents tend to demonstrate high cytotoxicity, while peptide-based reagents transfect with low efficiency. Xfect Protein Transfection Reagent offers the best advantages of both, i.e., it retains low cytotoxicity and delivers more protein to a higher percentage of target cells (see Image Data tab in the product details section below). Moreover, Xfect Protein Transfection Reagent can transfect cells that are growing at a higher density than competing products, which is important since assays are often performed within a few hours post-transfection and the higher cell densities ensure sufficient material for downstream analysis.

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Cat. #	Product	Size	Price
631323	Xfect™ Protein Transfection Reagent	30 Rxns	USD $502.00
The Xfect Protein Transfection Reagent allows the delivery of proteins and antibodies into cell lines in less than two hours. Delivery efficiency is high (60-99%), and proteins retain their biological activity. This kit contains Xfect Protein Transfection Reagent, Xfect Protein Buffer, deionized water, and a Beta-Galactosidase Control. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back 631323: Xfect Protein Transfection Reagent Back Simple, rapid protein transfection with Xfect Protein Transfection Reagent Simple, rapid protein transfection with Xfect Protein Transfection Reagent. Xfect’s reagent cell-penetrating activity enables proteins to be transported across membranes of mammalian cells. Back How fast is direct protein transfection? How fast is direct protein transfection?. We transfected human recombinant caspase-3 using Xfect Protein Transfection Reagent and detected completion of apoptosis after just 2 hr, demonstrating that protein transfection using Xfect reagent is both fast and delivers active protein (Panel B). Other methods for inducing apoptosis require 12 hr for completion of Annexin V staining (Martin, S. J. et al. (1995) J. Exp. Med. 182(5):1545–1556.). Back Xfect Protein Transfection Reagent delivers up to 8X more active beta-galactosidase into HeLa cells than the leading competitor, Product C. Xfect Protein Transfection Reagent delivers up to 8X more active beta-galactosidase into HeLa cells than the leading competitor, Product C. Panel A. Xfect Protein Transfection Reagent or Product C was used to transfect Jurkat cells with various amount of beta-galactosidase (beta-gal) according to the manufacturers’ recommended protocol. One hour post-transfection, the cells were assayed for beta-gal activity using ourLuminescent beta-galactosidase Detection Kit II (Cat. No. 631712). Xfect Protein Transfection Reagent displayed a far higher signal for beta-gal than did Product C. Panel B. HeLa cells transfected with 4 µg of beta-gal demonstrated higher efficiency and a higher amount of beta-gal protein per cell when transfection was performed with Xfect Protein Transfection Reagent (middle panel) than with Product C (right-hand panel). Back Xfect Protein Transfection Reagent delivers more recombinant fluorescent proteins than Product C into a range of cell types Xfect Protein Transfection Reagent delivers more recombinant fluorescent proteins than Product C into a range of cell types. Xfect Protein Transfection Reagent and Product C were used to transfect a number of cell lines with purified recombinant fluorescent protein (either rAcGFP1 or rDsRed-Express). In all of the cell lines tested, a far greater MFI (mean fluorescence intensity) was detected via flow cytometry after transfection with Xfect Protein Transfection Reagent than after transfection with Product C. Back Xfect Protein Transfection Reagent provides much higher cell viability than Product P when transfecting HeLa cells Xfect Protein Transfection Reagent provides much higher cell viability than Product P when transfecting HeLa cells. HeLa cells were seeded in a 96-well plate (3 x 10⁴ cells per well) one day prior to transfection. The cells were transfected with 0.25 µg of beta-galactosidase using Product P or Xfect Protein Transfection Reagent for 4 hours. A WST-1 proliferation assay was performed to assess cytotoxicity in the transfected samples relative to untreated HeLa cells. Back Protein transfection efficiencies across different cell lines: Xfect Protein Transfection Reagent vs. the leading competitor, Product C Protein transfection efficiencies across different cell lines: Xfect Protein Transfection Reagent vs. the leading competitor, Product C. Panel A. Xfect Protein Transfection Reagent yields higher transfection efficiencies than Product C across a broad range of mammalian cells, including a number of rodent and human cell lines, hard-to-transfect human suspension cells, and mouse embryonic stem cells. The cells were transfected with 4 µg of recombinant fluorescent protein (either rAcGFP1 or rDsRed-Express) using Xfect Protein Transfection Reagent or Product C according to the manufacturers’ recommended protocol. Transfection efficiency was assessed by flow cytometry one hour post-transfection. Panel B. HeLa transfected with 5 µg of rAcGFP1 using Product C or Xfect Protein Transfection Reagent.
631324	Xfect™ Protein Transfection Reagent	100 Rxns	USD $1072.00
The Xfect Protein Transfection Reagent allows the delivery of proteins and antibodies into cell lines in less than two hours. Delivery efficiency is high (60-99%), and proteins retain their biological activity. This kit contains Xfect Protein Transfection Reagent, Xfect Protein Buffer, deionized water, and a Beta-Galactosidase Control. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back Simple, rapid protein transfection with Xfect Protein Transfection Reagent Simple, rapid protein transfection with Xfect Protein Transfection Reagent. Xfect’s reagent cell-penetrating activity enables proteins to be transported across membranes of mammalian cells. Back How fast is direct protein transfection? How fast is direct protein transfection?. We transfected human recombinant caspase-3 using Xfect Protein Transfection Reagent and detected completion of apoptosis after just 2 hr, demonstrating that protein transfection using Xfect reagent is both fast and delivers active protein (Panel B). Other methods for inducing apoptosis require 12 hr for completion of Annexin V staining (Martin, S. J. et al. (1995) J. Exp. Med. 182(5):1545–1556.). Back Xfect Protein Transfection Reagent delivers up to 8X more active beta-galactosidase into HeLa cells than the leading competitor, Product C. Xfect Protein Transfection Reagent delivers up to 8X more active beta-galactosidase into HeLa cells than the leading competitor, Product C. Panel A. Xfect Protein Transfection Reagent or Product C was used to transfect Jurkat cells with various amount of beta-galactosidase (beta-gal) according to the manufacturers’ recommended protocol. One hour post-transfection, the cells were assayed for beta-gal activity using ourLuminescent beta-galactosidase Detection Kit II (Cat. No. 631712). Xfect Protein Transfection Reagent displayed a far higher signal for beta-gal than did Product C. Panel B. HeLa cells transfected with 4 µg of beta-gal demonstrated higher efficiency and a higher amount of beta-gal protein per cell when transfection was performed with Xfect Protein Transfection Reagent (middle panel) than with Product C (right-hand panel). Back Xfect Protein Transfection Reagent delivers more recombinant fluorescent proteins than Product C into a range of cell types Xfect Protein Transfection Reagent delivers more recombinant fluorescent proteins than Product C into a range of cell types. Xfect Protein Transfection Reagent and Product C were used to transfect a number of cell lines with purified recombinant fluorescent protein (either rAcGFP1 or rDsRed-Express). In all of the cell lines tested, a far greater MFI (mean fluorescence intensity) was detected via flow cytometry after transfection with Xfect Protein Transfection Reagent than after transfection with Product C. Back Xfect Protein Transfection Reagent provides much higher cell viability than Product P when transfecting HeLa cells Xfect Protein Transfection Reagent provides much higher cell viability than Product P when transfecting HeLa cells. HeLa cells were seeded in a 96-well plate (3 x 10⁴ cells per well) one day prior to transfection. The cells were transfected with 0.25 µg of beta-galactosidase using Product P or Xfect Protein Transfection Reagent for 4 hours. A WST-1 proliferation assay was performed to assess cytotoxicity in the transfected samples relative to untreated HeLa cells. Back Protein transfection efficiencies across different cell lines: Xfect Protein Transfection Reagent vs. the leading competitor, Product C Protein transfection efficiencies across different cell lines: Xfect Protein Transfection Reagent vs. the leading competitor, Product C. Panel A. Xfect Protein Transfection Reagent yields higher transfection efficiencies than Product C across a broad range of mammalian cells, including a number of rodent and human cell lines, hard-to-transfect human suspension cells, and mouse embryonic stem cells. The cells were transfected with 4 µg of recombinant fluorescent protein (either rAcGFP1 or rDsRed-Express) using Xfect Protein Transfection Reagent or Product C according to the manufacturers’ recommended protocol. Transfection efficiency was assessed by flow cytometry one hour post-transfection. Panel B. HeLa transfected with 5 µg of rAcGFP1 using Product C or Xfect Protein Transfection Reagent. Back 631324: Xfect Protein Transfection Reagent

Overview

Transfect a large amount of active protein
Virtually no cytotoxicity, unlike lipofection
Very high efficiency, even in stem or hematopoietic cells
Simple protocol—assay for your protein in just 2 hours

More Information

Applications

Cell cycle studies
Transcriptional regulation
Protein localization
Signaling pathway studies
Apoptosis
Oncogenesis
Epigenetics
Cell regeneration
Transdifferentiation

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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Protein transfection reagents

What is the Xfect Protein Transfection Reagent and how does it work?

Why transfect purified proteins directly?

What are the advantages of Xfect Transfection Reagent compared to other protein delivery technologies?

Notice to purchaser

631323: Xfect Protein Transfection Reagent

Simple, rapid protein transfection with Xfect Protein Transfection Reagent

How fast is direct protein transfection?

Xfect Protein Transfection Reagent delivers up to 8X more active beta-galactosidase into HeLa cells than the leading competitor, Product C.

Xfect Protein Transfection Reagent delivers more recombinant fluorescent proteins than Product C into a range of cell types

Xfect Protein Transfection Reagent provides much higher cell viability than Product P when transfecting HeLa cells

Protein transfection efficiencies across different cell lines: Xfect Protein Transfection Reagent vs. the leading competitor, Product C