New products from Takara Bio

Determine absolute copy number and DNA integrity with only a few nanograms of FFPE input using the new all-in-one kit

Takara FFPE DNA QC All-in-One Kit

• Perform simultaneous quantification and fragmentation evaluation by real‑time PCR—no electrophoresis required
• Achieve copy number and integrity data to adjust input or cycle number and obtain uniform, high-yield libraries with ThruPLEX and ThruPLEX FLEX kits
• Save precious material with the kit’s low-input capabilities (requires only a few ng of FFPE DNA vs. >100 ng typically needed for electrophoretic methods)

Rapidly detect DNA or RNA targets without needing to switch reagents or kits

BcaBEST Isothermal FluorDetect Kit (DNA/RNA) 

• Use one kit to amplify DNA or RNA targets under isothermal conditions (loop-mediated isothermal amplification [LAMP] or one-step RT-LAMP, respectively) 
• Detect targets in as little as 20 min via TB Green fluorescence monitoring  
• Prevent false positives with the inclusion of Uracil-N-Glycosylase (UNG) 

Facilitate rapid and efficient lentiviral transduction of T cells with a microfluidic-based sponge

Lenti-X T-Cell Transduction Sponge

• Conduct T‑cell activation and transduction in one step in isolated T-cells or PBMCs
• Streamline your protocol with a product preinfused with two anti-human antibodies and IL-2 cytokine—or add your own!
• Skip labor-intensive, time-consuming spinoculation or chemical enhancers
• Transduce 10⁵–10⁷ cells in one well of a 24-well plate with each sponge

Construct DNA libraries from both ssDNA and dsDNA—all from one kit

ssDNA-Seq Kit

• Use with highly fragmented, damaged, or degraded DNA, including FFPE samples
• Enable analysis of ssDNA fragments as short as 50 bp
• Complete library prep in under 2 hr with a simple workflow
• Multiplex up to 384 samples with Unique Dual Index Kit compatibility

Scale up mRNA production with low dsRNA byproducts

PrimeCap T7 RNA Polymerase (low dsRNA) GMP grade

• Obtain in-vitro transcribed RNA that is notably safer by reducing dsRNA byproducts by >90%
• Retain the same high yields as T7 RNA polymerase ver.2, especially when used with cap analogs such as CleanCap Reagent AG (TriLink Bio Technologies)
• Facilitate a smooth transition to large-scale manufacturing with enzymes produced following GMP guidelines

Isolate both RNA and DNA from tissue, plants, or cultured cells—all with one kit

NucleoMag RNA and NucleoMag RNA/DNA Pro

• Gain the flexibility to isolate RNA and DNA, or RNA only, from the same sample
• Process samples manually or streamline with automated magnetic rod systems
• Obtain highly pure RNA/DNA for direct use in downstream applications such as RT-PCR, enzymatic reactions, and sequencing

Unlock full-length RNA isoforms from low-input samples

SMART-Seq mRNA Long Read

• Capture full-length transcripts up to 8 kb in length
• Process ~10 pg–100 ng of total RNA input or samples consisting of 1–1,000 cells
• Multiplex up to 96 samples for sequencing on the Oxford Nanopore Technology platform

Increase RNA purification throughput for plant and fungi samples

NucleoSpin 96 RNA Plant & Fungi

• Process samples in a 96-well format by centrifugation, vacuum, or automation
• Adapt the protocol with ease for your specific plant or fungal sample type
• Remove secondary metabolites that interfere with downstream processing

Achieve rapid and sensitive mycoplasma extraction

NucleoSpin Mycoplasma DNA

• Extract even trace amounts of mycoplasma DNA successfully from cell culture samples
• Have full trust in your results as every lot is confirmed to test negative for mycoplasma DNA
• Pair with the TaKaRa Mycoplasma qPCR Detection Kit for a complete mycoplasma solution

Discover fast, thermal-cycle-free amplification with high yields

phi29 DNA Polymerase

• Perform high-yield, isothermal amplification from as little as 5 pg of DNA
• Obtain accurate amplification from long DNA templates
• Skip optimization of PCR primer and cycling conditions

Ultra-long amplification PCR enzyme with high fidelity and specificity

PrimeSTAR LongSeq DNA Polymerase

• Amplify ultra-long (50 kb or more) segments—even difficult sequences such as GC-/AT-rich sequences
• Suppress nonspecific amplification—for highly specific long-length multiplex PCR reactions
• Leverage highly accurate template amplification—ideal for long-read sequencing

Get more from your NGS data with our latest bioinformatics software

Cogent NGS Analysis Pipeline / Cogent NGS Discovery Software

• Demultiplex scDNA-seq data in addition to single-cell and bulk RNA-seq data
• Perform ribodepletion of data using SortMeRNA
• Analyze data generated from the high-throughput Shasta Total RNA-Seq and the Shasta Whole-Genome Amplification kits