BcaBEST Isothermal FluorDetect Kit (DNA/RNA)

Rapidly detect DNA or RNA targets without needing to switch reagents or kits. The BcaBEST Isothermal FluorDetect Kit (DNA/RNA) enables fluorescent detection of nucleic acid sequences using either loop-mediated isothermal amplification (LAMP) or one-step RT-LAMP.

The core of the kit is the BcaBEST Isothermal Mix, an optimized premix containing BcaBEST DNA Polymerase ver. 2.0 and PrimeScript III Reverse Transcriptase. This allows seamless amplification from both DNA and RNA templates. Detection is achieved through TB Green Solution, which enables real-time fluorescence monitoring using a PCR system equipped with a FAM filter. BcaBEST Isothermal Mix also includes Uracil-N-Glycosylase (UNG) to prevent false positives from carryover contamination.

To run the assay, simply combine your DNA or RNA template with standard LAMP primers, the BcaBEST Isothermal Mix, and TB Green Solution. Incubate the reaction at 63°C for 20 minutes using a real-time PCR system to detect and quantify amplification.

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Cat. #	Product	Size	Price
RR385A	BcaBEST™ Isothermal FluorDetect Kit (DNA/RNA)	100 Rxns	USD $263.00
The BcaBEST Isothermal FluorDetect Kit (DNA/RNA) allows for rapid detection of DNA or RNA targets via LAMP or one-step RT-LAMP. The kit includes BcaBEST Isothermal Mix (2X) and TB Green Solution (300X). Cat. # RR385A contains enough reagents for 100 reactions. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back BcaBEST Isothermal FluorDetect Kit (DNA/RNA) achieved rapid detection of DNA and RNA samples BcaBEST Isothermal FluorDetect Kit (DNA/RNA) achieved rapid detection of DNA and RNA samples. BcaBEST Isothermal FluorDetect Kit (DNA/RNA) was used to amplify DNA and RNA samples (template amount = 1, 10, 100, or 1,000 pg or 5, 50, 500, or 5,000 copies) at n = 2. The time to reach a constant fluorescence intensity was measured. For these samples, detection was generally achieved within 20 min. The "1/2" in the figure indicates that one of the n = 2 was not detected within 30 min. For the NTC (no-template control), no amplification within 30 min was observed except for one NTC for orf-134. RNA samples: HL-60 total RNA (ACTB and ALAS1) and SARS-CoV-2 RNA controls (Twist Bioscience). DNA samples: E. coli genomic DNA (fecA) and lambda phage genomic DNA (orf-134). Back BcaBEST Isothermal FluorDetect Kit (DNA/RNA) has a faster detection time compared to other companies’ reagents using total RNA input BcaBEST Isothermal FluorDetect Kit (DNA/RNA) has a faster detection time compared to other companies’ reagents using total RNA input. BcaBEST Isothermal FluorDetect Kit (DNA/RNA) and fluorescent LAMP detection reagents from various companies were used to amplify HL-60 total RNA (ACTB; template amount 1, 10, 100, or 1,000 pg) at n = 2. The time to reach a certain fluorescence intensity was measured. The BcaBEST Isothermal FluorDetect Kit (DNA/RNA) was capable of detection within 15 min. Back BcaBEST Isothermal FluorDetect Kit (DNA/RNA) has a slightly faster detection time compared to other companies’ reagents using nasopharyngeal fluid and urine inputs BcaBEST Isothermal FluorDetect Kit (DNA/RNA) has a slightly faster detection time compared to other companies’ reagents using nasopharyngeal fluid and urine inputs. BcaBEST Isothermal FluorDetect Kit (DNA/RNA) and fluorescent LAMP detection reagents from various companies were used to detect SARS CoV-2 (noninfectious; 1,000 copies/rxn) spiked into nasopharyngeal fluid (amount = 0, 0.1, or 1 μl/rxn) at n = 2, Chlamydia trachomatis (noninfectious; 180 copies/rxn) spiked into urine (amount = 0, 2.25, or 4.5 μl/rxn) at n = 3, and Neisseria gonorrhoeae (noninfectious; 180 copies/rxn) spiked into urine (amount = 0, 2.25, or 4.5 μl/rxn) at n = 3. The spiked samples were treated with Proteinase K and then heat inactivated before the assay. The time to reach a certain fluorescence intensity was measured. Back BcaBEST Isothermal FluorDetect Kit (DNA/RNA) is compatible with Q-Probe detection BcaBEST Isothermal FluorDetect Kit (DNA/RNA) is compatible with Q-Probe detection. BcaBEST Isothermal FluorDetect Kit (DNA/RNA) was used to amplify Chlamydia trachomatis control DNA (template amount = 1,000 or 200 copies/rxn at n = 2 and 0 copies/rxn at n = 6) and Neisseria gonorrhoeae control DNA (template amount = 1,000 or 200 copies/rxn at n = 2 and 0 copies/rxn at n = 6). The time for the fluorescence of TB Green (intercalator) or Q-Probe to reach the threshold were measured. Q-Probe hybridizes with amplified LAMP products, indicating decreasing fluorescence. The Q-Probe exhibits the same detection time as the intercalator but demonstrates more specificity in this assay.
RR385B	BcaBEST™ Isothermal FluorDetect Kit (DNA/RNA)	500 Rxns	USD $1118.00
The BcaBEST Isothermal FluorDetect Kit (DNA/RNA) allows for rapid detection of DNA or RNA targets via LAMP or one-step RT-LAMP. The kit includes BcaBEST Isothermal Mix (2X) and TB Green Solution (300X). Cat. # RR385B contains enough reagents for 500 reactions. Please refer to Cat. # RR385A for complete product documentation and resources. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Image Data Back BcaBEST Isothermal FluorDetect Kit (DNA/RNA) achieved rapid detection of DNA and RNA samples BcaBEST Isothermal FluorDetect Kit (DNA/RNA) achieved rapid detection of DNA and RNA samples. BcaBEST Isothermal FluorDetect Kit (DNA/RNA) was used to amplify DNA and RNA samples (template amount = 1, 10, 100, or 1,000 pg or 5, 50, 500, or 5,000 copies) at n = 2. The time to reach a constant fluorescence intensity was measured. For these samples, detection was generally achieved within 20 min. The "1/2" in the figure indicates that one of the n = 2 was not detected within 30 min. For the NTC (no-template control), no amplification within 30 min was observed except for one NTC for orf-134. RNA samples: HL-60 total RNA (ACTB and ALAS1) and SARS-CoV-2 RNA controls (Twist Bioscience). DNA samples: E. coli genomic DNA (fecA) and lambda phage genomic DNA (orf-134). Back BcaBEST Isothermal FluorDetect Kit (DNA/RNA) has a faster detection time compared to other companies’ reagents using total RNA input BcaBEST Isothermal FluorDetect Kit (DNA/RNA) has a faster detection time compared to other companies’ reagents using total RNA input. BcaBEST Isothermal FluorDetect Kit (DNA/RNA) and fluorescent LAMP detection reagents from various companies were used to amplify HL-60 total RNA (ACTB; template amount 1, 10, 100, or 1,000 pg) at n = 2. The time to reach a certain fluorescence intensity was measured. The BcaBEST Isothermal FluorDetect Kit (DNA/RNA) was capable of detection within 15 min. Back BcaBEST Isothermal FluorDetect Kit (DNA/RNA) has a slightly faster detection time compared to other companies’ reagents using nasopharyngeal fluid and urine inputs BcaBEST Isothermal FluorDetect Kit (DNA/RNA) has a slightly faster detection time compared to other companies’ reagents using nasopharyngeal fluid and urine inputs. BcaBEST Isothermal FluorDetect Kit (DNA/RNA) and fluorescent LAMP detection reagents from various companies were used to detect SARS CoV-2 (noninfectious; 1,000 copies/rxn) spiked into nasopharyngeal fluid (amount = 0, 0.1, or 1 μl/rxn) at n = 2, Chlamydia trachomatis (noninfectious; 180 copies/rxn) spiked into urine (amount = 0, 2.25, or 4.5 μl/rxn) at n = 3, and Neisseria gonorrhoeae (noninfectious; 180 copies/rxn) spiked into urine (amount = 0, 2.25, or 4.5 μl/rxn) at n = 3. The spiked samples were treated with Proteinase K and then heat inactivated before the assay. The time to reach a certain fluorescence intensity was measured. Back BcaBEST Isothermal FluorDetect Kit (DNA/RNA) is compatible with Q-Probe detection BcaBEST Isothermal FluorDetect Kit (DNA/RNA) is compatible with Q-Probe detection. BcaBEST Isothermal FluorDetect Kit (DNA/RNA) was used to amplify Chlamydia trachomatis control DNA (template amount = 1,000 or 200 copies/rxn at n = 2 and 0 copies/rxn at n = 6) and Neisseria gonorrhoeae control DNA (template amount = 1,000 or 200 copies/rxn at n = 2 and 0 copies/rxn at n = 6). The time for the fluorescence of TB Green (intercalator) or Q-Probe to reach the threshold were measured. Q-Probe hybridizes with amplified LAMP products, indicating decreasing fluorescence. The Q-Probe exhibits the same detection time as the intercalator but demonstrates more specificity in this assay.
9300A	TB Green® Solution	50 uL	USD $96.00
TB Green Solution contains a DNA intercalating dye that binds to double-stranded DNA, resulting in strong fluorescence upon binding. Its excitation and emission spectra are compatible with FAM detection channels, enabling real-time fluorescence monitoring and melting curve analysis in loop-mediated isothermal amplification (LAMP) assays. Supplied as a 300X concentrated solution. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components

Overview

Amplify DNA or RNA targets via isothermal conditions with one kit
Use with primers commonly used for LAMP (F3/B3/FIP/BIP)
Detect targets via fluorescence in as little as 20 min
Prevent false positives with the inclusion of UNG

More Information

Applications

LAMP and RT-LAMP
Rapid, point-of-care pathogen detection

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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BcaBEST Isothermal FluorDetect Kit (DNA/RNA)

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