PrimePath Direct Saliva SARS-CoV-2 Detection Kit

PrimePath Direct Saliva SARS-CoV-2 Detection Kit is a research-use-only, complete kit for detecting SARS-CoV-2 directly from saliva specimens without the need for any DNA/RNA extraction. The workflow has been thoroughly formulated to ensure safe sample handling, reduce hands-on time, and deliver faster, more sensitive results.

The kit contains all components required to collect the saliva specimens and run the RT-qPCR detection assay, including our high-performance One-Step PrimeScript III RT-qPCR Kit (Cat. # RR600A). This assay is based on primers and probes designed and published by the CDC.

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Cat. #	Product	Size	Price
638340	PrimePath™ Direct Saliva SARS-CoV-2 Detection Kit	200 Rxns	USD $1422.00
Complete end-to-end kit for collecting samples and running an RT-qPCR assay to detect SARS-CoV-2 in saliva without needing any DNA/RNA extraction or additional heat-lysis steps. NOTE: Cat. No. 638340 contains Cat. Nos. 638341–3 and RR600A. Customers have the option of purchasing these kit components separately, but this is not required with the purchase of Cat. No. 638340. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Workflow for PrimePath Direct Saliva SARS-CoV-2 Detection Kit No extraction step is needed to detect SARS-CoV-2 directly in a saliva sample. Use the provided collection sticks to obtain saliva samples. After inactivation, simply add RT-qPCR reagents directly to the saliva and load into a real-time PCR instrument. Back NextSWAB collection stick Manufactured with medical-grade plastic, NextSWAB will precisely capture ~50 μl saliva and provide a user-friendly experience. Back Activity of viruses in PrimePath Viral Inactivation Buffer Saliva samples are dissolved in PrimePath Viral Inactivation Buffer after collection by the NextSWAB. By 2 hours, the indicated viruses are inactivated, making them safe to handle or transport. (ND = not detected) Back Detection of the SARS-CoV-2 nucleocapsid gene by qPCR PrimePath SARS-CoV-2 Assay Mix was compared to that of Competitor E in a quantitative PCR (qPCR) assay. For both the N1 (FAM) and N2 (HEX) regions of the SARS-CoV-2 nucleocapsid gene, our reagent delivered greater fluorescence intensity, enabling easier detection of the pathogen. Back Detection of the SARS-CoV-2 nucleocapsid gene by ddPCR Both the N1 (FAM) and N2 (HEX) regions of the SARS-CoV-2 nucleocapsid gene were quantified using droplet digital PCR (ddPCR). At all dilutions tested, the copy number of each target was calculated to be a consistent value.
638343	PrimePath™ Saliva Collection Kit	200 Preps	USD $332.00
The PrimePath Saliva Collection Kit contains collection sticks and a viral inactivation buffer that is used both for storage and processing samples. At one end of each collection stick, there are channels for collecting liquid such as saliva. When mixed with the collected saliva sample, the buffer will inactivate any SARS-CoV-2 particles that are present. This process makes the saliva compatible with the RT-qPCR step in the PrimePath Saliva Direct SARS-CoV-2 Detection Kit. NOTE: Cat. No. 638343 is included with Cat. No. 638340. It can also be purchased separately. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Workflow for PrimePath Direct Saliva SARS-CoV-2 Detection Kit No extraction step is needed to detect SARS-CoV-2 directly in a saliva sample. Use the provided collection sticks to obtain saliva samples. After inactivation, simply add RT-qPCR reagents directly to the saliva and load into a real-time PCR instrument. Back NextSWAB collection stick Manufactured with medical-grade plastic, NextSWAB will precisely capture ~50 μl saliva and provide a user-friendly experience. Back Activity of viruses in PrimePath Viral Inactivation Buffer Saliva samples are dissolved in PrimePath Viral Inactivation Buffer after collection by the NextSWAB. By 2 hours, the indicated viruses are inactivated, making them safe to handle or transport. (ND = not detected)
RR600A	One Step PrimeScript™ III RT-PCR Kit	200 Rxns	USD $405.00
One Step PrimeScript III RT-qPCR Mix is a dedicated reagent for one-step real-time, probe-based RT-qPCR (using the 5’ nuclease method). This 2X premix does not freeze at its storage temperature of –20℃, so a reaction can be started simply by adding the template sample, primer, and a probe for detecting the desired target. The quick and simple protocol allows the reverse transcription and qPCR reactions to be performed in the same tube. The reverse transcription reaction uses the novel PrimeScript III RTase, which displays increased heat tolerance (up to 55℃) while maintaining the specificity and extensibility of PrimeScript RTase. This allows cDNA synthesis from RNA with a more complex secondary structure. After cDNA synthesis, TaKaRa Taq HS performs highly specific and efficient PCR amplification, while the fluorescence emitted by the probe is detected in real-time. One Step PrimeScript III RT-qPCR Mix is also highly resistant to a wide variety of inhibitory substances in blood and soil, allowing stable one-step real-time RT-qPCR to be performed on a wide range of samples. This product can be used for various applications such as gene expression, RNA virus detection, etc. Cat. # RR600A contains sufficient reagent for performing 200 reactions in a volume of 25 µl per reaction. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back RR600A: One Step PrimeScript III RT-PCR Kit

Overview

Complete kit—all components needed for the user to perform SARS-CoV-2 detection are included (except the qPCR instrument). The kit includes our high-performance One Step PrimeScript III RT-PCR Kit for a simple setup. The in vitro-transcribed (IVT) PrimePath SARS-CoV-2 Positive Control is validated using ddPCR and comprises exact copies of the target RNA sought by the CDC-designed primers and probes in this assay.
Easier, safer sample collection and handling—PrimePath Saliva Collection Kit provides unique collection sticks for easy capture of saliva samples. Additionally, the kit comes with a buffer that inactivates SARS-CoV-2 once the saliva sample is dissolved, making it safer for handling and transportation.
Faster results—no DNA/RNA extraction is necessary. A total qPCR assay detecting SARS-CoV-2 can be completed in <30 minutes.

More Information

Applications

Detection of SARS-CoV-2 in unpurified saliva samples

Additional Product Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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PrimePath Direct Saliva SARS-CoV-2 Detection Kit

Notice to purchaser

Workflow for PrimePath Direct Saliva SARS-CoV-2 Detection Kit

NextSWAB collection stick

Activity of viruses in PrimePath Viral Inactivation Buffer

Detection of the SARS-CoV-2 nucleocapsid gene by qPCR

Detection of the SARS-CoV-2 nucleocapsid gene by ddPCR

Notice to purchaser

Workflow for PrimePath Direct Saliva SARS-CoV-2 Detection Kit

NextSWAB collection stick

Activity of viruses in PrimePath Viral Inactivation Buffer

Notice to purchaser

RR600A: One Step PrimeScript III RT-PCR Kit

Overview

More Information

Applications

Additional Product Information