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  • ‹ Back to Ligation kits
  • DNA ligation kits, quick
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Home › Products › Cloning › Ligation kits › Mighty Cloning Reagent Set (Blunt End)

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Mighty Cloning Reagent Set (Blunt End)

Mighty Cloning Reagent Set (Blunt End) for preparation of PCR products for cloning into blunt-end vectors.

Mighty Cloning Reagent Set (Blunt End) is designed to allow PCR products to be quickly cloned into blunt-end vectors with a simple method. The blunting and 5'-phosphorylation reactions are done simultaneously and in parallel, so both blunt- and -dA ended products can be prepared in a single reaction. This kit eliminates the need for pretreatment (such as enzyme inactivation, removal of unused dNTPs and primers, etc.) of PCR products.

Cat. # Product Size Price License Quantity Details
6027 Mighty Cloning Reagent Set (Blunt End) 20 Rxns USD $424.00

Takara's Reagent Set for Mighty Cloning Kit (Blunt End) is designed to simply and quickly prepare PCR products for cloning into blunt-ended vectors. Because the blunting and 5'-phosphorylation reactions are done simultaneously, both blunt- and dA-ended products can be prepared in a single reaction. This kit eliminates the need for pre-treatment (such as enzyme inactivation, removal of unused dNTPs and primers, etc.), of PCR products. This kit includes the same ligation reagents as are included in Takara's DNA Ligation Kit (Mighty Mix), which provide simple, quick, and efficient ligation. The kit is suitable for blunt-end cloning of PCR products obtained with PrimeSTAR HS and e2TAK DNA Polymerases (not available in all regions).

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Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data Resources

Back

pUC118 Hinc II / BAPpUC118 DNA (BAP-treated) is a useful blunt-end cloning vector, prepared by cleavage with Hinc II, then dephosphorylated by alkaline phosphatase purified from E. coli

pUC118 Hinc II / BAPpUC118 DNA (BAP-treated) is a useful blunt-end cloning vector, prepared by cleavage with Hinc II, then dephosphorylated by alkaline phosphatase purified from E. coli

pUC118 Hinc II / BAP

pUC118 DNA (BAP-treated) is a useful blunt-end cloning vector, prepared by cleavage with Hinc II, then dephosphorylated by alkaline phosphatase purified from E.coli (BAP). The sequence of DNA cloned into this vector can be verified with M13 primer M4 and RV.

Vector map of pUC118

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Back

Cloning site of pUC118pUC118 Hinc II / BAPpUC118 DNA (BAP-treated) is a useful blunt-end cloning vector, prepared by cleavage with Hinc II, then dephosphorylated by alkaline phosphatase purified from E

Cloning site of pUC118pUC118 Hinc II / BAPpUC118 DNA (BAP-treated) is a useful blunt-end cloning vector, prepared by cleavage with Hinc II, then dephosphorylated by alkaline phosphatase purified from E

Cloning site of pUC118

pUC118 Hinc II / BAP

pUC118 DNA (BAP-treated) is a useful blunt-end cloning vector, prepared by cleavage with Hinc II, then dephosphorylated by alkaline phosphatase purified from E. coli (BAP). The sequence of DNA cloned into this vector can be verified with M13 primer M4 and RV.

.

Back

6027: Mighty Cloning Reagent Set (Blunt End)

6027: Mighty Cloning Reagent Set (Blunt End)

More Information

Applications

  • Quick and easy preparation of PCR products for cloning into blunt-end vectors

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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  • Human ACE2 stable cell line
  • Viral RNA isolation
  • Viral and host sequencing
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  • Publications
  • Next-generation sequencing
  • Spatial omics
  • RNA-seq
  • DNA-seq
  • Single-cell NGS automation
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  • Signature enzymes
  • High-throughput real-time PCR solutions
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  • Stem cells and stem cell-derived cells
  • mRNA and cDNA synthesis
  • In vitro transcription
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
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  • Purified total RNA and mRNA
  • PCR
  • Most popular polymerases
  • High-yield PCR
  • High-fidelity PCR
  • GC rich PCR
  • PCR master mixes
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  • In-Fusion seamless cloning
  • Competent cells
  • Ligation kits
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