T4 RNA Ligase

T4 RNA Ligase is a ligation enzyme for joining single-stranded RNA or DNA fragments by catalyzing the formation of phosphodiester bonds between single-stranded RNA or DNA containing 5'-phosphate and 3'-hydroxyl termini. This enzyme requires ATP for activity, and its activity is highest for RNA-RNA ligation, lower for RNA-DNA ligation, and lowest for DNA-DNA ligation. 3'-end labeling is often completed using 5'-[³²P]pCp.

Cat. #	Product	Size	Price
2050A	T4 RNA Ligase	1,000 Units	USD $244.00
T4 RNA Ligase catalyzes the formation of phosphodiester bonds between singlestranded nucleic acids with 5'-phosphate and 3'-hydroxyl termini. The enzyme requires ATP for activity. The rate of activity is highest for RNA-RNA ligation, lower for RNA-DNA ligation and very low for DNA-DNA ligation. T4 RNA Ligase is supplied in 20 mM Tris-HCl (pH 7.5), 50 mM NaCl, 1 mm DTT, 0.1 mM EDTA and 50% glycerol. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back 2050A: T4 RNA Ligase
2050B	T4 RNA Ligase	5,000 Units	USD $978.00
T4 RNA Ligase catalyzes the formation of phosphodiester bonds between singlestranded nucleic acids with 5'-phosphate and 3'-hydroxyl termini. The enzyme requires ATP for activity. The rate of activity is highest for RNA-RNA ligation, lower for RNA-DNA ligation and very low for DNA-DNA ligation. T4 RNA Ligase is supplied in 20 mM Tris-HCl (pH 7.5), 50 mM NaCl, 1 mm DTT, 0.1 mM EDTA and 50% glycerol. Cat. # 2050B contains 5 of Cat. # 2050A. Please refer to Cat. # 2050A for complete product documentation and resources. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Image Data Back 2050B: T4 RNA Ligase

More Information

Applications

Ligation of single-stranded RNA or DNA
3'-end labeling of single stranded RNA or DNA

Source

E. coli B strain infected with T4 am N82

Storage

–20°C

Concentration

10–50 U/µl

Unit definition

One unit is defined as the amount of enzyme that converts 1 pmol of [5'-³²P]pCp into its acid-insoluble form in 10 minutes at 5°C, using oligo(A) as the substrate during 3' end labeling of RNA.

Product citations

England, T. E., Bruce, A. G. & Uhlenbeck, O. C. Specific labeling of 3' termini of RNA with T4 RNA ligase. Methods Enzymol. 65, 65–74 (1980).

Romaniuk, P. J. & Uhlenbeck, O. C. Joining of RNA molecules with RNA ligase. Methods Enzymol. 100, 52–9 (1983).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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