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PrimeSTAR GXL DNA Polymerase

PrimeSTAR GXL polymerase is our most robust high-fidelity polymerase available for challenging targets (GC-rich sequences, long amplicons, excess template). It is a modified version of the PrimeSTAR HS enzyme that includes an elongation factor to provide unsurpassed processivity.

PrimeSTAR GXL polymerase is our most robust high-fidelity polymerase available for challenging targets (GC-rich sequences, excess template, long amplicons). It is a modified version of the PrimeSTAR HS enzyme that includes an elongation factor to provide unsurpassed processivity.

PrimeSTAR GXL DNA Polymerase provides efficient PCR amplification even for the toughest scenarios, including amplification of GC-rich targets, amplification in the presence of excess template that can contribute to nonspecific binding, and amplification of long products (up to 30 kb). The antibody-mediated hot-start feature enhances PCR specificity and sensitivity. PrimeSTAR GXL polymerase comes with separate tubes of 5X buffer and dNTP mixture.

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Cat. #	Product	Size	Price
R050A	PrimeSTAR® GXL DNA Polymerase	250 Units	USD $283.00
A hot-start, high-fidelity PCR enzyme, PrimeSTAR GXL DNA Polymerase excels in reactions with GC-rich templates, excess template, and long amplicons up to 30 kb (GXL). The polymerase is supplied with separate tubes of optimized buffer (Mg²⁺ plus), and dNTPs. Also available as a premix. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Resources Back Examples of product yield on GC-rich templates: comparison of PrimeSTAR GXL DNA Polymerase with four other commercially available high-fidelity DNA polymerases Examples of product yield on GC-rich templates: comparison of PrimeSTAR GXL DNA Polymerase with four other commercially available high-fidelity DNA polymerases. Company T’s enzyme includes buffers optimized for GC-rich templates. Back PrimeSTAR GXL DNA Polymerase amplifies products up to 30 kb (human genomic DNA template), 40 kb (lambda DNA), or 13.5 kb (human cDNA) PrimeSTAR GXL DNA Polymerase amplifies products up to 30 kb (human genomic DNA template), 40 kb (lambda DNA), or 13.5 kb (human cDNA). Back PrimeSTAR GXL DNA Polymerase shows efficient amplification in reaction mixes with a wide range of template quantity, including high levels of template that inhibit the activity of other commercially available high fidelity DNA polymerases PrimeSTAR GXL DNA Polymerase shows efficient amplification in reaction mixes with a wide range of template quantity, including high levels of template that inhibit the activity of other commercially available high fidelity DNA polymerases. Back R050A: PrimeSTAR GXL DNA Polymerase
R050B	PrimeSTAR® GXL DNA Polymerase	1,000 Units	USD $892.00
A hot-start, high-fidelity PCR enzyme, PrimeSTAR GXL DNA Polymerase excels in reactions with GC-rich templates, excess template, and long amplicons up to 30 kb (GXL). The polymerase is supplied with separate tubes of optimized buffer (Mg²⁺ plus), and dNTPs. Cat. # R050B contains 4 of Cat. # R050A. Please refer to Cat. # R050A for complete product documentation and resources. Also available as a premix. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back Examples of product yield on GC-rich templates: comparison of PrimeSTAR GXL DNA Polymerase with four other commercially available high-fidelity DNA polymerases Examples of product yield on GC-rich templates: comparison of PrimeSTAR GXL DNA Polymerase with four other commercially available high-fidelity DNA polymerases. Company T’s enzyme includes buffers optimized for GC-rich templates. Back PrimeSTAR GXL DNA Polymerase amplifies products up to 30 kb (human genomic DNA template), 40 kb (lambda DNA), or 13.5 kb (human cDNA) PrimeSTAR GXL DNA Polymerase amplifies products up to 30 kb (human genomic DNA template), 40 kb (lambda DNA), or 13.5 kb (human cDNA). Back PrimeSTAR GXL DNA Polymerase shows efficient amplification in reaction mixes with a wide range of template quantity, including high levels of template that inhibit the activity of other commercially available high fidelity DNA polymerases PrimeSTAR GXL DNA Polymerase shows efficient amplification in reaction mixes with a wide range of template quantity, including high levels of template that inhibit the activity of other commercially available high fidelity DNA polymerases. Back R050B: PrimeSTAR GXL DNA Polymerase

Overview

Amplification of products up to 30 kb (human genomic DNA), 40 kb (lambda DNA), or 13.5 kb (human cDNA)
Antibody-mediated hot-start formulation
High tolerance for GC-rich templates as well as the presence of excess nucleic acids templates

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Applications

Reliable amplification of long, GC-rich, or AT-rich sequences, such as regulatory regions and repeats
HLA typing
Unbiased library amplification for NGS

Termini of PCR products

PrimeSTAR GXL DNA Polymerase generates products with blunt ends. It is suitable for use in conjunction with blunt-ended cloning systems.

Recommended maximum amplicon length

Up to 30 kb (human genomic DNA template)
Up to 40 kb (lambda DNA template)
Up to 13.5 kb (human cDNA template)

Bulk, custom, and OEM information

If you are interested in bulk purchasing, custom packaging, custom formulations (including glycerol-free and high concentration), or partnership opportunities, please contact Corporate Development at oem@takarabio.com to discuss your needs or visit our OEM page to submit an inquiry.

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

1.02 | Quantifying population size by the hairs of the bears

Asian black bears live in the Himalayas, parts of Asia, and the Japanese islands Honshu and Shikoku. Related to now-extinct bear species and classified as vulnerable, Asian black bears are of keen interest to conservation biologists. But they're also aggressive, and researchers weren't eager to get up close and personal. So, how to estimate population size accurately while minimizing bear interaction? Hair traps and a sensitive PCR assay using PrimeSTAR GXL polymerase. That's Good Science!

Learn more about DNA Polymerases

Tech notes

Superior amplification of GC-rich templates with PrimeSTAR GXL DNA Polymerase

View data on the amplification of GC-rich templates without reaction optimization or inclusion of additives such as DMSO or Betaine.

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See what our customers are saying about PrimeSTAR GXL DNA Polymerase!

Hard to clone >65% GC rich clones. This product also was great for challenging genotype PCR reactions. I will be using this for my point mutation PCR cloning."
—Blake Nichols, UNIV OF CALIF DAVIS

I tried different Taq to amplify a fragment with a high GC content (70%) and this is the only one that worked!"
—Tatiana Ruiz Larroya, ACTIVE MOTIF INC.

It is a great enzyme. I love it! It is very good on pcr out long fragments, and i have tried other enzymes which can not do as good as PrimeStar GXL DNA polymerase on long PCRs. My lab have purchased several packs for this good enzyme."
—Katherine Tao, UNIV OF UTAH

Find high-quality solutions for applications downstream of your PCR

Looking for quick and dependable purification of your amplicon? Easily clean up your PCR product, either from an agarose gel or directly from the reaction.

Plan on using your amplicon for cloning? Seamlessly insert your PCR product into any vector, at any site of linearization using ligation-independent cloning.

Clean it

Find answers to your PCR questions

Primer design

Frequently asked questions about primer design for successful PCR.

Optimization

Frequently asked questions about PCR optimization.

Troubleshooting

Frequently asked questions about troubleshooting your PCR problems.

Applications and conditions

Frequently asked questions about general and specific applications for PCR and which polymerases to choose.

Shipping, storage, and handling

Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.

Avoid DNA contamination in PCR

There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.

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PrimeSTAR GXL DNA Polymerase

Notice to purchaser

Examples of product yield on GC-rich templates: comparison of PrimeSTAR GXL DNA Polymerase with four other commercially available high-fidelity DNA polymerases