Titanium Taq SP—high-yield DNA polymerase

Titanium Taq SP DNA Polymerase is a highly sensitive, robust, hot-start enzyme for use in PCR applications. It is especially useful in demanding multiplex assays. It contains the same Titanium Taq DNA Polymerase and TaqStart Antibody as our standard Titanium Taq products, but with a modified, license-free buffer formulation that is suitable for commercial and OEM use, including molecular diagnostic services and kits. The following versions of Titanium Taq SP are available:

A glycerol-free version (Cat. # 638517), which is ideal for preparation of custom lyophilized (dried-down) PCR master mixes that offer higher convenience and room-temperature stability
A lyophilized, EcoDry premix version (Cat. # 638519) enables high performance with minimal effort and maximum ease, provided as a prealiquoted lyophilized master mix that already contains all the reagents needed for high-yield PCR in each tube
A registered General Purpose Reagent (GPR) version (Cat. # 639292) for molecular diagnostic development and testing
A GMP-grade version (Cat. # R320A)* that is manufactured as a quality-assured product under GMP conditions

*Not available in all geographic locations. Check for availability in your area.

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Cat. #	Product	Size	Price
638517	50X Titanium® Taq SP (Glycerol-Free)	250 uL	$361.00
Titanium Taq SP (Glycerol-Free) DNA polymerase contains Takara's high-yield Titanium Taq polymerase mix, which is made up of a 5′ to 3′ exonuclease-deficient Taq polymerase and TaqStart Antibody for hot-start PCR. Glycerol-free formulation is ideal for preparation of custom dried-down PCR master mixes that offer higher convenience and room-temperature stability. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back 638517: 50X Titanium Taq SP (Glycerol-Free)
639292	Titanium® Taq SP DNA Polymerase	100 Rxns	$145.00
Titanium Taq SP DNA Polymerase contains the same hot-start, high-yield Titanium Taq DNA polymerase and TaqStart Antibody as the standard Titanium Taq (Cat. # 639208). Cat. # 639292 is a registered general purpose reagent (GPR) appropriate for use in general laboratory applications, including molecular diagnostic development and testing. Notice to purchaser This product is a general purpose reagent intended For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated. This product is not intended for a specific application or made for any particular clinical use. The performance characteristics of this product have not been fully established. It is the user’s responsibility to validate the performance of the product, and any component thereof, for any particular use. Resale or transfer of this product, any component thereof, or any substance produced through use of this product, or any component thereof, to any third party is expressly forbidden. To obtain additional rights, please contact your local office or go to our website for additional information. Documents Components Image Data Back Titanium Taq efficiently amplifies specific genes from genomic DNA *Titanium Taq* efficiently amplifies specific genes from genomic DNA.** Human cardiac beta-myosin heavy chain fragments of different lengths were amplified from 100 ng of genomic DNA using Titanium Taq and two leading competitor’s hot start Taq polymerases. Optimal conditions were used for each enzyme, as specified by the manufacturer. Lane 1: 0.5 kb fragment. Lane 2: 1 kb fragment. Lane 3: 1.8 kb fragment. Lane M: 1 kb DNA size ladder. Back TITANIUM Taq is active over a wide range of Mg2+ concentrations *TITANIUM Taq* is active over a wide range of Mg²⁺ concentrations.** TITANIUM was used to amplify a 500 bp region of Calf Thymus genomic DNA. The MgCl₂ concentration was varied as indicated. The enzyme performed consistently through the range of Mg²⁺ used. Lane M: DNA size marker.
R320A	Titanium® Taq SP DNA Polymerase GMP grade	100 Rxns	$178.00
Titanium Taq SP DNA Polymerase GMP grade is manufactured as a quality-assured product under GMP conditions. These manufacturing practices bring an added level of confidence and consistency to this highly sensitive, robust, hot-start enzyme for use in PCR applications that require high yields. This SP version contains a modified, license-free buffer formulation that is suitable for commercial and OEM use. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components
638519	Titanium® Taq SP EcoDry™ Premix	48 Rxns	$87.00
This lyophilized PCR master mix is based on Takara's high-yield Titanium Taq DNA polymerase. This premix contains all of the necessary reagents for hot-start, high-yield PCR. EcoDry premixes are packaged as individual 8-well tube strips with optically clear sealing caps. Simply reconstitute the premix by adding PCR-grade water along with primers and template. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components

Overview

Reduce background amplification by using fewer PCR cycles
Save time by skipping optimization—Titanium Taq tolerates a wide range of Mg²⁺ concentrations
Amplify targets of up to 2 kb from highly complex templates, such as mammalian genomic DNA
Amplify rare or low-copy targets
Conveniently set up your PCR at room temperature and see increased specificity and yield due to the integrated hot-start antibody
Prepare your own lyophilized PCR master mixes with the glycerol-free version (Cat. # 638517)
The eco-friendly, lyophilized premix is ideal for room-temperature storage and convenience (Cat. # 638519)
Bring high performance to commercial and OEM use with cost-savings on royalty and license fees in some situations. (Contact Corporate Development at bd_oem@takarabio.com to discuss your needs or visit our OEM page to submit an inquiry.)

More Information

Applications

Genotyping
Whole-genome PCR
Multiplex PCR
Primer extension
Hot-start PCR
Preparative PCR
Rare-template amplification
Master mix lyophilization with glycerol-free 50X Titanium Taq SP (Cat. # 638517)

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Tech notes

Titanium Taq outperforms other polymerases in high-throughput genotyping

Titanium Taq DNA Polymerase is unrivaled for multiplex PCR in high-throughput genotyping applications.

Learn more about whole-genome SNP genotyping with Titanium Taq

Find answers to your PCR questions

Primer design

Frequently asked questions about primer design for successful PCR.

Optimization

Frequently asked questions about PCR optimization.

Troubleshooting

Frequently asked questions about troubleshooting your PCR problems.

Applications and conditions

Frequently asked questions about general and specific applications for PCR and which polymerases to choose.

Shipping, storage, and handling

Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.

Avoid DNA contamination in PCR

There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.

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