Titanium Taq SP—high-yield DNA polymerase
Titanium Taq SP DNA Polymerase is a highly sensitive, robust, hot-start enzyme for use in PCR applications. It is especially useful in demanding multiplex assays. It contains the same Titanium Taq DNA Polymerase and TaqStart Antibody as our standard Titanium Taq products, but with a modified, license-free buffer formulation that is suitable for commercial and OEM use, including molecular diagnostic services and kits. The following versions of Titanium Taq SP are available:
Titanium Taq SP DNA Polymerase is a highly sensitive, robust, hot-start enzyme for use in PCR applications. It is especially useful in demanding multiplex assays. It contains the same Titanium Taq DNA Polymerase and TaqStart Antibody as our standard Titanium Taq products, but with a modified, license-free buffer formulation that is suitable for commercial and OEM use, including molecular diagnostic services and kits. The following versions of Titanium Taq SP are available:
- A glycerol-free version (Cat. # 638517), which is ideal for preparation of custom lyophilized (dried-down) PCR master mixes that offer higher convenience and room-temperature stability
- A lyophilized, EcoDry premix version (Cat. # 638519) enables high performance with minimal effort and maximum ease, provided as a prealiquoted lyophilized master mix that already contains all the reagents needed for high-yield PCR in each tube
- A registered General Purpose Reagent (GPR) version (Cat. # 639292) for molecular diagnostic development and testing
-
A GMP-grade version (Cat. # R320A)* that is manufactured as a quality-assured product under GMP conditions
*Not available in all geographic locations. Check for availability in your area.
Overview
- Reduce background amplification by using fewer PCR cycles
- Save time by skipping optimization—Titanium Taq tolerates a wide range of Mg2+ concentrations
- Amplify targets of up to 2 kb from highly complex templates, such as mammalian genomic DNA
- Amplify rare or low-copy targets
- Conveniently set up your PCR at room temperature and see increased specificity and yield due to the integrated hot-start antibody
- Prepare your own lyophilized PCR master mixes with the glycerol-free version (Cat. # 638517)
- The eco-friendly, lyophilized premix is ideal for room-temperature storage and convenience (Cat. # 638519)
- Bring high performance to commercial and OEM use with cost-savings on royalty and license fees in some situations. (Contact Corporate Development at bd_oem@takarabio.com to discuss your needs or visit our OEM page to submit an inquiry.)
More Information
Applications
- Genotyping
- Whole-genome PCR
- Multiplex PCR
- Primer extension
- Hot-start PCR
- Preparative PCR
- Rare-template amplification
- Master mix lyophilization with glycerol-free 50X Titanium Taq SP (Cat. # 638517)
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Tech notes
Titanium Taq outperforms other polymerases in high-throughput genotyping
Titanium Taq DNA Polymerase is unrivaled for multiplex PCR in high-throughput genotyping applications.
Learn more about whole-genome SNP genotyping with Titanium Taq
Find answers to your PCR questions
Primer design
Frequently asked questions about primer design for successful PCR.
Optimization
Frequently asked questions about PCR optimization.
Troubleshooting
Frequently asked questions about troubleshooting your PCR problems.
Applications and conditions
Frequently asked questions about general and specific applications for PCR and which polymerases to choose.
Shipping, storage, and handling
Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.
Avoid DNA contamination in PCR
There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.
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