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Home › Learning centers › Nucleic acid purification › Plasmid purification › NucleoBond Xtra Midi/Maxi

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Tech Note

NucleoBond Xtra Midi/Maxi/EF

A new generation of anion exchange plasmid purification kits—ultra-fast, endotoxin-free plasmid DNA with high yields

  • Plasmid DNA containing less than 0.05 EU/μg—transfect highly sensitive cells
  • Patented endotoxin removal via an additional washing step—no extra ice incubation needed
  • Optimized column design—midipreps in approximately 45 min, maxipreps in 50 min
  • Column filter included—high filter flow rates, lysate clearing and column loading in parallel
  • Improved silica matrix—typical yields of 400 μg (midi) and 1,000 μg (maxi)
  • NucleoBond Xtra Plus EF kits contain NucleoBond Finalizers to speed up DNA precipitation
NucleoBond Xtra Combi Rack for maxi and midi columns

Figure 1. The NucleoBond Xtra Combi Rack holds both maxi and midi columns.

Yield and prep time Transfection Sensitive cells Protocol

Yield and prep time  

Higher plasmid yields in less time compared to competitor anion exchange-based kits

When the yields and speed of NucleoBond Xtra Midi or Midi Plus kits and NucleoBond Xtra Maxi or Maxi Plus kits were compared to competitor anion exchange-based kits (Figure 2), the NucleoBond Xtra kits reduced preparation time by up to 60% and provided up to 100% higher yields than the competitor products.

NucleoBond Xtra kit yields and speed vs. a competitor

Figure 2. Comparison of NucleoBond Xtra kits' yields and speed to those of competitor products. Plasmid DNA was isolated following each manufacturer's protocol using the maximum culture volume with high plasmid content. The yield of plasmid DNA was determined after DNA precipitation. NucleoBond Xtra and competitor kits including a desalting tool were compared in Panel A, left side (midi) and Panel A, right side (maxi), while kits without a desalting tool (requiring centrifugation for DNA precipitation) were compared in Panel B, left side (midi) and Panel B, right side (maxi). Note that the yields of plasmid DNA are slightly lower for kits including the desalting tool than for kits without the desalting tool, due to the residual DNA remaining on the tool.

Transfection  

Higher transfection efficiencies than a competitor kit

NucleoBond Xtra Maxi and Product Q were each used to purify a 6-kb high-copy plasmid expressing gephyrin (a polypeptide associated with the postsynaptic receptor complex that plays a key role in the organization of the postsynaptic membrane) fused to an EGFP (enhanced green fluorescent protein) reporter. When each plasmid prep was transfected into HEK293 cells, which showed transient expression of gephyrin, higher transfection efficiencies were observed with NucleoBond Xtra Maxi than Product Q (Figure 3, compare Panels A and B).

Transfection efficiencies with NucleoBond Xtra Maxi vs. a competitor

Figure 3. Comparison of transfection efficiencies using plasmid DNA purified with NucleoBond Xtra Maxi and a competitor product. A 6-kb high-copy plasmid expressing gephyrin fused to an EGFP reporter was transfected into HEK293 cells after purification with NucleoBond Xtra Maxi (Panel A) or Product Q (Panel B). Plasmid purification from E. coli DH5α was performed according to each manufacturers' instructions using the maximum culture volume with high plasmid content. NucleoBond Xtra Maxi yielded 1,222 μg of plasmid with an A260/280 of 1.84. Product Q yielded 557 μg of plasmid with an A260/280 of 1.86. HEK293 cells in 12-well plates (2.5 x 104 cells/well) were transfected for 48 hr with 0.1–0.25 μg/well of plasmid DNA using FuGENE HD/6 (Roche, 1.5 μl/well). Data kindly provided by Prof. Dr. Guenter Schwarz, Institute of Biochemistry, University of Cologne, Germany.

Sensitive cells  

Transfection of highly sensitive primary neurons

NucleoBond Xtra EF was used to purify a 9-kb high-copy plasmid expressing neuroligin (a postsynaptic transmembrane protein) fused to a GFP reporter. When the purified plasmid was transfected into primary rat hippocampal neurons, which are highly sensitive to endotoxins, fluorescence microscopy showed transient expression of neuroligin (Figure 4).

NucleoBond Xtra Maxi EF-purified plasmid transfects primary neurons

Figure 4. Transfection of primary rat hippocampal neurons using plasmid DNA purified with NucleoBond Xtra EF. A 9-kb high-copy plasmid expressing neuroligin fused to a GFP reporter was transfected into primary rat hippocampal neurons after purification with NucleoBond Xtra EF. Plasmid purification from E. coli XL1-Blue was performed according to the manufacturer's instructions. Primary rat hippocampal neurons were cultured in vitro for six days and transfected with 1 µg of plasmid DNA per well using calcium phosphate. The neurons were visualized by fluorescence microscopy 48 hr post-transfection. Data kindly provided by Dr. Nina Wittenmayer, Ruprecht-Karls-University Heidelberg, Institute for Anatomy and Cell Biology, Dept. of Medical Cell Biology, Heidelberg, Germany.

Endotoxin-free DNA for transfection of primary rat hippocampal neurons

A 4.5-kb high-copy plasmid purified using NucleoBond Xtra Maxi Plus EF was shown to transfect primary rat hippocampal neurons using fluorescence microscopy, as seen in both panels of Figure 5.

Endotoxin-free DNA transfects primary rat hippocampal neurons

Figure 5. Endotoxin-free DNA for transfection of primary rat hippocampal neurons. A 4.5-kb high-copy plasmid was transfected into primary rat hippocampal neurons after purification from E. coli DH5α with NucleoBond Xtra Maxi Plus EF. Primary rat hippocampal neurons were cultured in vitro for 14 days and transfected with 1 µg of plasmid DNA per well using NeuroMag transfection reagent (OZ Biosciences). The neurons were visualized by fluorescence microscopy 48 hr post-transfection. Data kindly provided by OZ Biosciences, Marseille, France.

Protocol  

No extra incubation is needed for endotoxin removal

The NucleoBond Xtra EF procedure, which is very similar to the NucleoBond Xtra procedure, requires no extra incubation step for endotoxin removal (Figure 6).

Comparing NucleoBond Xtra and NucleoBond Xtra EF procedures

Figure 6. Comparison of the NucleoBond Xtra and NucleoBond Xtra EF procedures.

Related Products

Cat. # Product Size Price License Quantity Details
740424.10 NucleoBond® Xtra Maxi EF 10 Preps USD $333.00

NucleoBond Xtra Maxi EF (10) 10 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Maxi Columns, NucleoBond Xtra Maxi Column Filters, buffers, RNase A

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns

The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns
The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns. Combi Racks facilitate column handling, loading, and elution.

Back

The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin)

The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin)
The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin). The first washing step using equilibration buffer is performed with the column filter inserted into the column to wash out residual lysate from the filter and obtain maximum recovery of DNA. After subsequent washing, the purified plasmid DNA is eluted in a high-salt buffer and precipitated with isopropanol. Precipitation can be performed by centrifugation [NucleoBond Xtra Midi (midiprep) or Maxi (maxiprep) kits] or by using the NucleoBond Finalizer.

Back

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification
NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE.

Back

740424.10: NucleoBond Xtra Maxi EF

740424.10: NucleoBond Xtra Maxi EF
740424.50 NucleoBond® Xtra Maxi EF 50 Preps USD $1660.00

NucleoBond Xtra Maxi EF (50) 50 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Maxi Columns, NucleoBond Xtra Maxi Column Filters, buffers, RNase A

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

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NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification
NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE.

Back

The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns

The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns
The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns. Combi Racks facilitate column handling, loading, and elution.

Back

The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin)

The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin)
The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin). The first washing step using equilibration buffer is performed with the column filter inserted into the column to wash out residual lysate from the filter and obtain maximum recovery of DNA. After subsequent washing, the purified plasmid DNA is eluted in a high-salt buffer and precipitated with isopropanol. Precipitation can be performed by centrifugation [NucleoBond Xtra Midi (midiprep) or Maxi (maxiprep) kits] or by using the NucleoBond Finalizer.

Back

740424.50: NucleoBond Xtra Maxi EF

740424.50: NucleoBond Xtra Maxi EF
740426.50 NucleoBond® Xtra Maxi Plus EF 50 Preps USD $1875.00

NucleoBond Xtra Maxi Plus EF (50) 50 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Maxi Columns, NucleoBond Xtra Maxi Column Filters, NucleoBond Finalizers Large, syringes, buffers, RNase A

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

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NucleoBond Xtra Finalizers: Finalizers are provided with the Plus kits

NucleoBond Xtra Finalizers: Finalizers are provided with the Plus kits
NucleoBond Xtra Finalizers: Finalizers are provided with the Plus kits. NucleoBond Finalizer technology replaces the tedious and time-consuming centrifugation step that follows every isopropanol precipitation of diluted DNA samples.

Back

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification
NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE.

Back

The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin)

The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin)
The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin). The first washing step using equilibration buffer is performed with the column filter inserted into the column to wash out residual lysate from the filter and obtain maximum recovery of DNA. After subsequent washing, the purified plasmid DNA is eluted in a high-salt buffer and precipitated with isopropanol. Precipitation can be performed by centrifugation [NucleoBond Xtra Midi (midiprep) or Maxi (maxiprep) kits] or by using the NucleoBond Finalizer.

Back

740426.50: NucleoBond Xtra Maxi Plus EF

740426.50: NucleoBond Xtra Maxi Plus EF
740426.10 NucleoBond® Xtra Maxi Plus EF 10 Preps USD $393.00

NucleoBond Xtra Maxi Plus EF (10) 10 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Maxi Columns, NucleoBond Xtra Maxi Column Filters, NucleoBond Finalizers Large, syringes, buffers, RNase A

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

Back

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification
NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE.

Back

NucleoBond Xtra Finalizers: Finalizers are provided with the Plus kits

NucleoBond Xtra Finalizers: Finalizers are provided with the Plus kits
NucleoBond Xtra Finalizers: Finalizers are provided with the Plus kits. NucleoBond Finalizer technology replaces the tedious and time-consuming centrifugation step that follows every isopropanol precipitation of diluted DNA samples.

Back

740426.10: NucleoBond Xtra Maxi Plus EF

740426.10: NucleoBond Xtra Maxi Plus EF
740420.10 NucleoBond® Xtra Midi EF 10 Preps USD $155.00

NucleoBond Xtra Midi EF (10) 10 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Midi Columns, NucleoBond Xtra Midi Column Filters, buffers, RNase A

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

Back

The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns

The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns
The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns. Combi Racks facilitate column handling, loading, and elution.

Back

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification
NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE.

Back

The NucleoBond PC endotoxin-free plasmid purification protocol compared to that of a leading competitor

The NucleoBond PC endotoxin-free plasmid purification protocol compared to that of a leading competitor
The NucleoBond PC endotoxin-free plasmid purification protocol compared to that of a leading competitor.

Back

740420.10: NucleoBond Xtra Midi EF

740420.10: NucleoBond Xtra Midi EF

Back

Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF

Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF

Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF. Multiple plasmid preps were performed in parallel from bacterial cultures containing varying quantities of cells (ODV200 vs. ODV600) using NucleoBond Xtra Midi, NucleoBond Xtra Midi EF, and the ZymoPURE II Plasmid Midiprep Kit, and analyzed by agarose gel electrophoresis. At both input levels, the NucleoBond Xtra kits provided higher and more consistent yields than the ZymoPURE II kit, and this performance advantage was particularly striking for the higher input amount (ODV400). ODV was calculated by multiplying the optical density of each culture (measured at 600 nm) times the culture volume.

740420.50 NucleoBond® Xtra Midi EF 50 Preps USD $710.00

NucleoBond Xtra Midi EF (50) 50 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Midi Columns, NucleoBond Xtra Midi Column Filters, buffers, RNase A

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

Back

The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns

The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns
The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns. Combi Racks facilitate column handling, loading, and elution.

Back

The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin)

The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin)
The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin). The first washing step using equilibration buffer is performed with the column filter inserted into the column to wash out residual lysate from the filter and obtain maximum recovery of DNA. After subsequent washing, the purified plasmid DNA is eluted in a high-salt buffer and precipitated with isopropanol. Precipitation can be performed by centrifugation [NucleoBond Xtra Midi (midiprep) or Maxi (maxiprep) kits] or by using the NucleoBond Finalizer.

Back

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification
NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE.

Back

The NucleoBond PC endotoxin-free plasmid purification protocol compared to that of a leading competitor

The NucleoBond PC endotoxin-free plasmid purification protocol compared to that of a leading competitor
The NucleoBond PC endotoxin-free plasmid purification protocol compared to that of a leading competitor.

Back

740420.50: NucleoBond Xtra Midi EF

740420.50: NucleoBond Xtra Midi EF

Back

Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF

Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF

Reliable plasmid purification from large quantities of cells with NucleoBond Xtra Midi and NucleoBond Xtra Midi EF. Multiple plasmid preps were performed in parallel from bacterial cultures containing varying quantities of cells (ODV200 vs. ODV600) using NucleoBond Xtra Midi, NucleoBond Xtra Midi EF, and the ZymoPURE II Plasmid Midiprep Kit, and analyzed by agarose gel electrophoresis. At both input levels, the NucleoBond Xtra kits provided higher and more consistent yields than the ZymoPURE II kit, and this performance advantage was particularly striking for the higher input amount (ODV400). ODV was calculated by multiplying the optical density of each culture (measured at 600 nm) times the culture volume.

740422.10 NucleoBond® Xtra Midi Plus EF 10 Preps USD $194.00

NucleoBond Xtra Midi Plus EF (10) 10 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Midi Columns, NucleoBond Xtra Midi Column Filters, NucleoBond Finalizers, syringes, buffers, RNase A

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

Back

The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns

The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns
The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns. Combi Racks facilitate column handling, loading, and elution.

Back

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification
NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE.

Back

NucleoBond Xtra Finalizers: Finalizers are provided with the Plus kits

NucleoBond Xtra Finalizers: Finalizers are provided with the Plus kits
NucleoBond Xtra Finalizers: Finalizers are provided with the Plus kits. NucleoBond Finalizer technology replaces the tedious and time-consuming centrifugation step that follows every isopropanol precipitation of diluted DNA samples.

Back

The NucleoBond PC endotoxin-free plasmid purification protocol compared to that of a leading competitor

The NucleoBond PC endotoxin-free plasmid purification protocol compared to that of a leading competitor
The NucleoBond PC endotoxin-free plasmid purification protocol compared to that of a leading competitor.

Back

740422.10: NucleoBond Xtra Midi Plus EF

740422.10: NucleoBond Xtra Midi Plus EF
740422.50 NucleoBond® Xtra Midi Plus EF 50 Preps USD $840.00

NucleoBond Xtra Midi Plus EF (50) 50 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Midi Columns, NucleoBond Xtra Midi Column Filters, NucleoBond Finalizers, syringes, buffers, RNase A

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

Back

The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns

The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns
The NucleoBond Xtra Combi Rack is specifically designed to support both Midi and Maxi columns. Combi Racks facilitate column handling, loading, and elution.

Back

The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin)

The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin)
The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion exchange resin). The first washing step using equilibration buffer is performed with the column filter inserted into the column to wash out residual lysate from the filter and obtain maximum recovery of DNA. After subsequent washing, the purified plasmid DNA is eluted in a high-salt buffer and precipitated with isopropanol. Precipitation can be performed by centrifugation [NucleoBond Xtra Midi (midiprep) or Maxi (maxiprep) kits] or by using the NucleoBond Finalizer.

Back

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification

NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification
NucleoBond Xtra Columns: The enlarged columns lead to lower silica resin beds and enable faster flow of lysate and buffers through the columns, allowing for fast purification. The improved silica material is based on the anion-exchange group MAE.

Back

NucleoBond Xtra Finalizers: Finalizers are provided with the Plus kits

NucleoBond Xtra Finalizers: Finalizers are provided with the Plus kits
NucleoBond Xtra Finalizers: Finalizers are provided with the Plus kits. NucleoBond Finalizer technology replaces the tedious and time-consuming centrifugation step that follows every isopropanol precipitation of diluted DNA samples.

Back

The NucleoBond PC endotoxin-free plasmid purification protocol compared to that of a leading competitor

The NucleoBond PC endotoxin-free plasmid purification protocol compared to that of a leading competitor
The NucleoBond PC endotoxin-free plasmid purification protocol compared to that of a leading competitor.

Back

740422.50: NucleoBond Xtra Midi Plus EF

740422.50: NucleoBond Xtra Midi Plus EF

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Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

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Alzheimer's disease research

Alzheimer's disease research

Alzheimer's disease (AD) affects ~5.8 million adults and is the sixth leading cause of death in the US alone. Over 400 AD clinical trials are currently under way, investigating the possibilities of immunotherapy, our microbiome, and inflammation as prospective therapeutic targets. We have a variety of best-in-class products that can help your AD research move forward.

See how our best-in-class solutions can help to advance your AD research

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Capturem Trypsin for a rapid, efficient mass spectometry workflow at room temperature.

Speed up your mass spec workflow

Capturem Trypsin provides rapid, efficient, and complete digestion of protein samples, allowing an uninterrupted mass spectometry workflow at room temperature for downstream protein analysis. This product utilizes our novel Capturem technology in a spin column format with membrane-immobilized trypsin. Capturem Trypsin Columns may be used to completely digest protein samples in less than a minute with digestion efficiencies (protein coverage) comparable to or better than those obtained using in-solution trypsin digestion.

Capturem trypsin technology

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Takara Bio's award-winning GMP-compliant manufacturing facility in Kusatsu, Shiga, Japan.

Partner with Takara Bio!

Takara Bio is proud to offer GMP-grade manufacturing capabilities at our award-winning facility in Kusatsu, Shiga, Japan.

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What does it take to generate good science? Careful planning, dedicated researchers, and the right tools. At Takara Bio, we thoughtfully develop best-in-class products to tackle your most challenging research problems, and have an expert team of technical support professionals to help you along the way, all at superior value.

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Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

Clontech, TaKaRa, cellartis

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