Higher plasmid yields in less time compared to competitor anion exchange-based kits
When the yields and speed of NucleoBond Xtra Midi or Midi Plus kits and NucleoBond Xtra Maxi or Maxi Plus kits were compared to competitor anion exchange-based kits (Figure 2), the NucleoBond Xtra kits reduced preparation time by up to 60% and provided up to 100% higher yields than the competitor products.
Higher transfection efficiencies than a competitor kit
NucleoBond Xtra Maxi and Product Q were each used to purify a 6-kb high-copy plasmid expressing gephyrin (a polypeptide associated with the postsynaptic receptor complex that plays a key role in the organization of the postsynaptic membrane) fused to an EGFP (enhanced green fluorescent protein) reporter. When each plasmid prep was transfected into HEK293 cells, which showed transient expression of gephyrin, higher transfection efficiencies were observed with NucleoBond Xtra Maxi than Product Q (Figure 3, compare Panels A and B).
Transfection of highly sensitive primary neurons
NucleoBond Xtra EF was used to purify a 9-kb high-copy plasmid expressing neuroligin (a postsynaptic transmembrane protein) fused to a GFP reporter. When the purified plasmid was transfected into primary rat hippocampal neurons, which are highly sensitive to endotoxins, fluorescence microscopy showed transient expression of neuroligin (Figure 4).
Endotoxin-free DNA for transfection of primary rat hippocampal neurons
A 4.5-kb high-copy plasmid purified using NucleoBond Xtra Maxi Plus EF was shown to transfect primary rat hippocampal neurons using fluorescence microscopy, as seen in both panels of Figure 5.
No extra incubation is needed for endotoxin removal
The NucleoBond Xtra EF procedure, which is very similar to the NucleoBond Xtra procedure, requires no extra incubation step for endotoxin removal (Figure 6).