We use cookies to improve your browsing experience and provide meaningful content. Read our cookie policy. Accept
  •  Customer Login
  • Register
  •  View Cart (0)
  •  Customer Login
  • Register
  •  View Cart (0)

Takara Bio
  • Products
  • Services & Support
  • Learning centers
  • APPLICATIONS
  • About
  • Contact Us

Clontech Takara Cellartis

Close

  • ‹ Back to Tips and troubleshooting
  • Scaling up: moving from research to large-scale RNA production for mRNA therapeutics
  • Why lyo-ready mixes are crucial for qPCR assay development
  • It's a snap! 9 considerations for easy multi-fragment cloning
  • Best practices for RNA-seq: Optimizing sample prep
  • Designing primers for site-directed mutagenesis
  • Qualities to look for in your ideal OEM partner
  • Understanding viral titration—behind the science
  • 4 factors to consider for immune repertoire profiling
  • 5 FACS tips for scRNA-seq
  • Choosing a his-tagged purification resin
  • 5 tips to make your single-cell RNA-seq experiments a success
  • Using UMIs in NGS experiments
  • Web and mobile apps
  • One-step vs. two-step RT-qPCR
  • Avoid DNA contamination in PCR
  • When your his-tagged constructs don't bind
Polyhistidine tag Troubleshooting issues with his-tag binding
His-tag purification learning center His-tag purification learning center
Home › About › BioView blog › Tips and troubleshooting › Choosing a his-tagged purification resin

BioView blog

  • Automation
    • Methods of detection for AMR surveillance
    • Optimizing agriculture screening with high-throughput genotyping
    • ICELL8 cx Single-Cell System and combinatorial indexing
    • Full-length scRNA-seq in white adipose tissue
    • Takara Bio Single-Cell Workshop, Spring 2021
    • Big problems from small bugs
    • Better biobanking with high-throughput qPCR
    • Top 5 considerations when automating single-cell sequencing
    • What's inside automated single-cell RNA-seq platforms?
  • Cancer research
    • Arc-well: sequence single-cell DNA from 30-year old FFPE samples
    • When Epstein-Barr virus becomes a chronic menace
    • Amplifying our understanding of breast cancer metastases
    • Cancer immunotherapy
    • Accurate detection of SNVs and CNVs from 5-cell inputs
  • Career spotlights
    • Career spotlight: territory manager
    • Career spotlight: senior inside sales representative
    • Career spotlight: manufacturing engineer
    • Career spotlight: senior lead development representative
    • Career spotlight: technical support scientist
  • Current events
    • National Hispanic Heritage Month
      • 2024
        • Dr. Nick Silva discusses his passion for training and supporting underrepresented students in our first National Hispanic Heritage Month spotlight interview
        • Dr. Loyda Morales Rodriguez discusses her Hispanic heritage and how it drives her passion for increasing diversity and inclusivity in clinical research
        • Dr. Darya Marchany-Rivera speaks about her passion for helping people—including Hispanic students—meet their goals and dreams
        • Dr. Dianne Laboy Cintrón talks about her experience as an underrepresented student—and studying an “underrepresented” part of the human genome
      • 2023
        • Dr. Jose Barbosa
        • Dr. Yadira Soto-Feliciano
        • Dr. Sarah Stewart
    • Identifying promising HIV vaccine strategies
    • Your spit can save your life
    • Controlling the spread of COVID-19 with direct saliva testing
    • The 2020 Nobel Prize in Chemistry for CRISPR
    • In memory of Hermann Bujard
    • Coronavirus publication: know your enemy
    • Dethroning king coronavirus with novel vaccines
    • Entering new worlds while staying in place
    • Working hard to meet production demands for SARS-CoV-2 testing
    • Shelter in place
    • Takara Bio plays a crucial role in fighting the novel coronavirus
    • Screening for novel coronavirus with one-step RT-qPCR: getting ahead of the outbreak
  • Customer stories
    • PrimeSTAR GXL: a decisive component in characterizing viral RNA structures
    • Joining the fight against antibiotic resistance
    • Overcoming technical challenges in extracellular vesicle research
    • Sequencing grey matter
    • Unlocking cardiomyocyte heterogeneity: the role of transcription factors
    • Profiling transcription factors with CUT&RUN sequencing
  • Gene editing
    • Measure twice, cut once
    • Successful knockout experiments part II
    • Successful knockout experiments part I
    • Efficient nonviral T-cell engineering using CRISPR
  • Research news
    • wellDA-seq: bridging the gap between genetic and epigenetic profiling
    • Engineered PsCas9 for therapeutic genome editing
    • Delivery of functionalized DNA origami into the cell nucleus
    • Demystifying and simplifying the lentiviral production and transduction workflow
    • On-demand pharmaceuticals in space
    • Amplify NGS libraries without bias
    • Takara Bio wins a Crabby!
    • A new hiPSC model for type 2 diabetes
    • TCR-seq methods: strengths, weaknesses, and rankings
    • A faster path to analysis for mAbs as therapeutic agents
    • Gene therapy takes a step forward
    • ICELL8 technology keeps cardiovascular research pumping
    • Women's Networking Event at AGBT 2020
    • Detecting infectious disease threats in a changing climate
    • Unraveling the world of microorganisms
    • Microbiomes in the brain and belly
    • Gaining insight into pulmonary arterial hypertension with purified exosomes
    • Total RNA-seq from human biofluids and EVs
    • Seq-ing the small
    • Taking the SMARTer approach to RNA-seq of FFPE tissues
  • Single-cell analysis
    • Optimized full-length single nuclei RNA sequencing (snRNA-seq) to propel crop innovation
    • ABRF publishes single-cell RNA-seq benchmarking study
    • Two RNA-seq approaches reveal resident memory T cells hold the power to reverse liver fibrosis
    • Combination of single-cell RNA-seq approaches yields insights into the brain
    • Combining droplet and full-length sequencing technologies for a complete picture
    • Smashing single-cell sequencing sensitivity
    • Change of heart: exploring transcriptional variation in cardiomyocytes
    • Accelerating chromatin mapping with single-cell ATAC-seq
    • Bringing epigenomic profiling to the single-cell biology stage
    • Using the power of RNA-seq to characterize brain cell types
  • Stem cell research
    • Tools for iPSC-derived disease model development
    • 20 years of human stem cell research
    • Maximize transduction efficiency in hematopoietic cells
  • Tips and troubleshooting
    • Scaling up: moving from research to large-scale RNA production for mRNA therapeutics
    • Why lyo-ready mixes are crucial for qPCR assay development
    • It's a snap! 9 considerations for easy multi-fragment cloning
    • Best practices for RNA-seq: Optimizing sample prep
    • Designing primers for site-directed mutagenesis
    • Qualities to look for in your ideal OEM partner
    • Understanding viral titration—behind the science
    • 4 factors to consider for immune repertoire profiling
    • 5 FACS tips for scRNA-seq
    • Choosing a his-tagged purification resin
    • 5 tips to make your single-cell RNA-seq experiments a success
    • Using UMIs in NGS experiments
    • Web and mobile apps
    • One-step vs. two-step RT-qPCR
    • Avoid DNA contamination in PCR
    • When your his-tagged constructs don't bind
  • Women in STEM
    • Women in Science Day 2022
    • Women in STEM interview: Christina Chang
    • Women in STEM interview: Kim Smith
  • That's Good Support!
  • About our blog
Need help?
Contact Sales
Polyhistidine tag Troubleshooting issues with his-tag binding
His-tag purification learning center His-tag purification learning center

How to choose the right his-tagged purification resin

Date: June 17, 2019

Author: Takara Bio Blog Team

Categories: Protein science | Useful resources

In the grand scheme of your research, the resin you use to purify your construct seems like one of the more trivial decisions. After all, the resin your labmate uses seems to work great. However, every protein construct is a little different, so the resin that works for the rest of your lab may not be the right one for your project. His-tagged proteins are purified using immobilized metal affinity chromatography (IMAC) resins, which come in many flavors of metal cores—the most popular being nickel- (Ni2+) or cobalt-based (Co2+) resins. Which one is the right choice for your next his-tagged protein purification? The answer lies in determining whether yield or purity is more crucial for your downstream applications.

The differences in performance of Co2+- and Ni2+-based IMAC resins is a result of the spatial positioning of each metal ion in the reactive core influencing the specificity and capacity of histidine binding (because, as all protein researchers know, structure impacts function!) Cobalt-based resins offer better specificity, resulting in higher purity, while nickel-based resins offer better adsorption, resulting in higher binding capacity. Each type of resin has its advantages and limitations, depending on your specific application.

Relationship between specificity versus adsorption of metals used in IMAC resins

Cobalt-based resin

Advantages

  • Highest purity
  • Highest specificity for his-tagged proteins
  • Reduced copurification of impurities

Applications

  • Crystallography
  • Functional assays
  • Structural studies

Structure of a cobalt-based resin

The reactive core of a cobalt-based resin has strict requirements for the spatial positioning of histidines, so only proteins containing adjacent histidines or specially positioned neighboring histidines are able to bind cobalt in this reactive core, resulting in higher purity and lower yields. Use a cobalt-based resin if you need extremely pure protein for highly sensitive methods such as crystallography and functional assays.

Nickel-based resin

Advantages

  • Highest binding capacity
  • Works under native or denaturing conditions
  • Low leakage of Ni2+ ions

Applications

  • Protein labeling
  • Antibody production
  • Animal studies

Structure of a nickel-IDA resin

The reactive core of a nickel-based resin has less strict spatial requirements, so it binds histidines located in places other than the protein's polyhistidine tag, resulting in higher yields and lower purity. Nickel-based resins are commonly available with iminodiacetic acid (IDA) and nitrilotriacetic acid (NTA) as the chelating ligand, which differ in how strongly they coordinate with the metal ion. IDA has three coordination sites with the Ni2+ ion, leaving three open sites for histidine residues to interact with the ion. NTA, on the other hand, has four coordination sites with the Ni2+ ion, leaving only two sites for histidine residues. Therefore, IDA-based resins have higher binding capacities. However, it is critical to note that the weaker coordination between IDA and the Ni2+ ion also leads to an increased likelihood of leaching of the metal into the purification buffers, which can negatively impact some downstream applications. If your main objective is to purify large amounts of protein for labeling experiments and antibody production, then a nickel-based resin is the better choice.

Summary

As an early step in protein research, choosing the right IMAC resin for your his-tagged protein purification is essential for achieving the best results for your downstream applications. It is difficult to predict which resin will work best for a given construct ahead of time, so the best approach is typically to test a few resins in small-scale batch purifications and assess which combination of yield and purity works best for your situation. To learn more about choosing the best his-tagged protein purification method for your experiment, read our his-tagged protein purification overview.

Back to Blog Front


Capturem membrane technology

Capturem technology

There is a constant need for faster, more efficient antibody and protein purification processes at any scale. Capturem membrane technology allows for purification directly from complex matrices, such as cell supernatants, in minutes. This technology also provides highly purified and concentrated antibodies and his-tagged proteins, even from samples containing additives not compatible with other purification technologies.

Antibody purification His-tagged purification

Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2025 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

Takara Bio

Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

Support
  • Contact us
  • Technical support
  • Customer service
  • Shipping & delivery
  • Sales
  • Feedback
Products
  • New products
  • Special offers
  • Instrument & reagent services
Learning centers
  • NGS
  • Gene function
  • Stem cell research
  • Protein research
  • PCR
  • Cloning
  • Nucleic acid purification
About
  • Our brands
  • Careers
  • Events
  • Blog
  • Need help?
  • Announcements
  • Quality and compliance
  • That's Good Science!
Facebook Twitter  LinkedIn

logo strip white

©2025 Takara Bio Inc. All Rights Reserved.

Region - North America Privacy Policy Terms and Conditions Terms of Use

Top



  • COVID-19 research
  • Viral detection with qPCR
  • SARS-CoV-2 pseudovirus
  • Human ACE2 stable cell line
  • Viral RNA isolation
  • Viral and host sequencing
  • Vaccine development
  • CRISPR screening
  • Drug discovery
  • Immune profiling
  • Publications
  • Next-generation sequencing
  • Spatial omics
  • RNA-seq
  • DNA-seq
  • Single-cell NGS automation
  • Reproductive health
  • Bioinformatics tools
  • Immune profiling
  • Real-time PCR
  • Great value master mixes
  • Signature enzymes
  • High-throughput real-time PCR solutions
  • Detection assays
  • References, standards, and buffers
  • Stem cell research
  • Media, differentiation kits, and matrices
  • Stem cells and stem cell-derived cells
  • mRNA and cDNA synthesis
  • In vitro transcription
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • PCR
  • Most popular polymerases
  • High-yield PCR
  • High-fidelity PCR
  • GC rich PCR
  • PCR master mixes
  • Cloning
  • In-Fusion seamless cloning
  • Competent cells
  • Ligation kits
  • Restriction enzymes
  • Nucleic acid purification
  • Automated platforms
  • Plasmid purification kits
  • Genomic DNA purification kits
  • DNA cleanup kits
  • RNA purification kits
  • Gene function
  • Gene editing
  • Viral transduction
  • Fluorescent proteins
  • T-cell transduction and culture
  • Tet-inducible expression systems
  • Transfection reagents
  • Cell biology assays
  • Protein research
  • Purification products
  • Two-hybrid and one-hybrid systems
  • Mass spectrometry reagents
  • Antibodies and ELISAs
  • Primary antibodies and ELISAs by research area
  • Fluorescent protein antibodies
  • New products
  • Special offers
  • OEM
  • Portfolio
  • Process
  • Facilities
  • Request samples
  • FAQs
  • Instrument services
  • Apollo services
  • ICELL8 services
  • SmartChip ND system services
  • Gene and cell therapy manufacturing services
  • Services
  • Facilities
  • Our process
  • Resources
  • Customer service
  • Sales
  • Make an appointment with your sales rep
  • Shipping & delivery
  • Technical support
  • Feedback
  • Online tools
  • GoStix Plus FAQs
  • Partnering & Licensing
  • Vector information
  • Vector document overview
  • Vector document finder
Takara Bio's award-winning GMP-compliant manufacturing facility in Kusatsu, Shiga, Japan.

Partner with Takara Bio!

Takara Bio is proud to offer GMP-grade manufacturing capabilities at our award-winning facility in Kusatsu, Shiga, Japan.

  • Automation systems
  • Shasta Single Cell System introduction
  • SmartChip Real-Time PCR System introduction
  • ICELL8 introduction
  • Next-generation sequencing
  • RNA-seq
  • Technical notes
  • Technology and application overviews
  • FAQs and tips
  • DNA-seq protocols
  • Bioinformatics resources
  • Webinars
  • Spatial biology
  • Real-time PCR
  • Download qPCR resources
  • Overview
  • Reaction size guidelines
  • Guest webinar: extraction-free SARS-CoV-2 detection
  • Technical notes
  • Nucleic acid purification
  • Nucleic acid extraction webinars
  • Product demonstration videos
  • Product finder
  • Plasmid kit selection guide
  • RNA purification kit finder
  • mRNA and cDNA synthesis
  • mRNA synthesis
  • cDNA synthesis
  • PCR
  • Citations
  • PCR selection guide
  • Technical notes
  • FAQ
  • Cloning
  • Automated In-Fusion Cloning
  • In-Fusion Cloning general information
  • Primer design and other tools
  • In‑Fusion Cloning tips and FAQs
  • Applications and technical notes
  • Stem cell research
  • Overview
  • Protocols
  • Technical notes
  • Gene function
  • Gene editing
  • Viral transduction
  • T-cell transduction and culture
  • Inducible systems
  • Cell biology assays
  • Protein research
  • Capturem technology
  • Antibody immunoprecipitation
  • His-tag purification
  • Other tag purification
  • Expression systems
  • Antibodies and ELISA
  • Molecular diagnostics
  • Interview: adapting to change with Takara Bio
  • Applications
  • Solutions
  • Partnering
  • Contact us
  • mRNA and protein therapeutics
  • Characterizing the viral genome and host response
  • Identifying and cloning protein targets
  • Expressing and purifying protein targets
  • Immunizing mice and optimizing vaccines
  • Pathogen detection
  • Sample prep
  • Detection methods
  • Identification and characterization
  • SARS-CoV-2
  • Antibiotic-resistant bacteria
  • Food crop pathogens
  • Waterborne disease outbreaks
  • Viral-induced cancer
  • Immunotherapy research
  • T-cell therapy
  • Antibody therapeutics
  • T-cell receptor profiling
  • TBI initiatives in cancer therapy
  • Cancer research
  • Kickstart your cancer research with long-read sequencing
  • Sample prep from FFPE tissue
  • Sample prep from plasma
  • Cancer biomarker quantification
  • Single cancer cell analysis
  • Cancer transcriptome analysis
  • Cancer genomics and epigenomics
  • HLA typing in cancer
  • Gene editing for cancer therapy/drug discovery
  • Alzheimer's disease research
  • Antibody engineering
  • Sample prep from FFPE tissue
  • Single-cell sequencing
  • Reproductive health technologies
  • Embgenix FAQs
  • Preimplantation genetic testing
  • ESM partnership program
  • ESM Collection Kit forms
  • Infectious diseases
  • Develop vaccines for HIV
Create a web account with us

Log in to enjoy additional benefits

Want to save this information?

An account with takarabio.com entitles you to extra features such as:

•  Creating and saving shopping carts
•  Keeping a list of your products of interest
•  Saving all of your favorite pages on the site*
•  Accessing restricted content

*Save favorites by clicking the star () in the top right corner of each page while you're logged in.

Create an account to get started

  • BioView blog
  • Automation
  • Cancer research
  • Career spotlights
  • Current events
  • Customer stories
  • Gene editing
  • Research news
  • Single-cell analysis
  • Stem cell research
  • Tips and troubleshooting
  • Women in STEM
  • That's Good Support!
  • About our blog
  • That's Good Science!
  • SMART-Seq Pro Biomarker Discovery Contest
  • DNA extraction educational activity
  • That's Good Science Podcast
  • Season one
  • Season two
  • Season three
  • Our brands
  • Our history
  • In the news
  • Events
  • Biomarker discovery events
  • Calendar
  • Conferences
  • Speak with us
  • Careers
  • Company benefits
  • Trademarks
  • License statements
  • Quality statement
  • HQ-grade reagents
  • International Contacts by Region
  • United States and Canada
  • China
  • Japan
  • Korea
  • Europe
  • India
  • Affiliates & distributors
  • Need help?
  • Privacy request
  • Website FAQs

That's GOOD Science!

What does it take to generate good science? Careful planning, dedicated researchers, and the right tools. At Takara Bio, we thoughtfully develop exceptional products to tackle your most challenging research problems, and have an expert team of technical support professionals to help you along the way, all at superior value.

Explore what makes good science possible

 Customer Login
 View Cart (0)
Takara Bio
  • Home
  • Products
  • Services & Support
  • Learning centers
  • APPLICATIONS
  • About
  • Contact Us
  •  Customer Login
  • Register
  •  View Cart (0)

Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

Clontech, TaKaRa, cellartis

  • Products
  • COVID-19 research
  • Next-generation sequencing
  • Real-time PCR
  • Stem cell research
  • mRNA and cDNA synthesis
  • PCR
  • Cloning
  • Nucleic acid purification
  • Gene function
  • Protein research
  • Antibodies and ELISA
  • New products
  • Special offers
  • COVID-19 research
  • Viral detection with qPCR
  • SARS-CoV-2 pseudovirus
  • Human ACE2 stable cell line
  • Viral RNA isolation
  • Viral and host sequencing
  • Vaccine development
  • CRISPR screening
  • Drug discovery
  • Immune profiling
  • Publications
  • Next-generation sequencing
  • Spatial omics
  • RNA-seq
  • DNA-seq
  • Single-cell NGS automation
  • Reproductive health
  • Bioinformatics tools
  • Immune profiling
  • Real-time PCR
  • Great value master mixes
  • Signature enzymes
  • High-throughput real-time PCR solutions
  • Detection assays
  • References, standards, and buffers
  • Stem cell research
  • Media, differentiation kits, and matrices
  • Stem cells and stem cell-derived cells
  • mRNA and cDNA synthesis
  • In vitro transcription
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • PCR
  • Most popular polymerases
  • High-yield PCR
  • High-fidelity PCR
  • GC rich PCR
  • PCR master mixes
  • Cloning
  • In-Fusion seamless cloning
  • Competent cells
  • Ligation kits
  • Restriction enzymes
  • Nucleic acid purification
  • Automated platforms
  • Plasmid purification kits
  • Genomic DNA purification kits
  • DNA cleanup kits
  • RNA purification kits
  • Gene function
  • Gene editing
  • Viral transduction
  • Fluorescent proteins
  • T-cell transduction and culture
  • Tet-inducible expression systems
  • Transfection reagents
  • Cell biology assays
  • Protein research
  • Purification products
  • Two-hybrid and one-hybrid systems
  • Mass spectrometry reagents
  • Antibodies and ELISA
  • Primary antibodies and ELISAs by research area
  • Fluorescent protein antibodies
  • Services & Support
  • OEM
  • Instrument services
  • Gene and cell therapy manufacturing
  • Customer service
  • Sales
  • Shipping & delivery
  • Technical support
  • Feedback
  • Online tools
  • Partnering & Licensing
  • Vector information
  • OEM
  • Portfolio
  • Process
  • Facilities
  • Request samples
  • FAQs
  • Instrument services
  • Apollo services
  • ICELL8 services
  • SmartChip ND system services
  • Gene and cell therapy manufacturing
  • Services
  • Facilities
  • Our process
  • Resources
  • Sales
  • Make an appointment with your sales rep
  • Online tools
  • GoStix Plus FAQs
  • Vector information
  • Vector document overview
  • Vector document finder
  • Learning centers
  • Automation systems
  • Next-generation sequencing
  • Spatial biology
  • Real-time PCR
  • Nucleic acid purification
  • mRNA and cDNA synthesis
  • PCR
  • Cloning
  • Stem cell research
  • Gene function
  • Protein research
  • Antibodies and ELISA
  • Automation systems
  • Shasta Single Cell System introduction
  • SmartChip Real-Time PCR System introduction
  • ICELL8 introduction
  • Next-generation sequencing
  • RNA-seq
  • Technical notes
  • Technology and application overviews
  • FAQs and tips
  • DNA-seq protocols
  • Bioinformatics resources
  • Webinars
  • Real-time PCR
  • Download qPCR resources
  • Overview
  • Reaction size guidelines
  • Guest webinar: extraction-free SARS-CoV-2 detection
  • Technical notes
  • Nucleic acid purification
  • Nucleic acid extraction webinars
  • Product demonstration videos
  • Product finder
  • Plasmid kit selection guide
  • RNA purification kit finder
  • mRNA and cDNA synthesis
  • mRNA synthesis
  • cDNA synthesis
  • PCR
  • Citations
  • PCR selection guide
  • Technical notes
  • FAQ
  • Cloning
  • Automated In-Fusion Cloning
  • In-Fusion Cloning general information
  • Primer design and other tools
  • In‑Fusion Cloning tips and FAQs
  • Applications and technical notes
  • Stem cell research
  • Overview
  • Protocols
  • Technical notes
  • Gene function
  • Gene editing
  • Viral transduction
  • T-cell transduction and culture
  • Inducible systems
  • Cell biology assays
  • Protein research
  • Capturem technology
  • Antibody immunoprecipitation
  • His-tag purification
  • Other tag purification
  • Expression systems
  • APPLICATIONS
  • Molecular diagnostics
  • mRNA and protein therapeutics
  • Pathogen detection
  • Immunotherapy research
  • Cancer research
  • Alzheimer's disease research
  • Reproductive health technologies
  • Infectious diseases
  • Molecular diagnostics
  • Interview: adapting to change with Takara Bio
  • Applications
  • Solutions
  • Partnering
  • Contact us
  • mRNA and protein therapeutics
  • Characterizing the viral genome and host response
  • Identifying and cloning protein targets
  • Expressing and purifying protein targets
  • Immunizing mice and optimizing vaccines
  • Pathogen detection
  • Sample prep
  • Detection methods
  • Identification and characterization
  • SARS-CoV-2
  • Antibiotic-resistant bacteria
  • Food crop pathogens
  • Waterborne disease outbreaks
  • Viral-induced cancer
  • Immunotherapy research
  • T-cell therapy
  • Antibody therapeutics
  • T-cell receptor profiling
  • TBI initiatives in cancer therapy
  • Cancer research
  • Kickstart your cancer research with long-read sequencing
  • Sample prep from FFPE tissue
  • Sample prep from plasma
  • Cancer biomarker quantification
  • Single cancer cell analysis
  • Cancer transcriptome analysis
  • Cancer genomics and epigenomics
  • HLA typing in cancer
  • Gene editing for cancer therapy/drug discovery
  • Alzheimer's disease research
  • Antibody engineering
  • Sample prep from FFPE tissue
  • Single-cell sequencing
  • Reproductive health technologies
  • Embgenix FAQs
  • Preimplantation genetic testing
  • ESM partnership program
  • ESM Collection Kit forms
  • Infectious diseases
  • Develop vaccines for HIV
  • About
  • BioView blog
  • That's Good Science!
  • Our brands
  • Our history
  • In the news
  • Events
  • Careers
  • Trademarks
  • License statements
  • Quality and compliance
  • HQ-grade reagents
  • International Contacts by Region
  • Need help?
  • Website FAQs
  • BioView blog
  • Automation
  • Cancer research
  • Career spotlights
  • Current events
  • Customer stories
  • Gene editing
  • Research news
  • Single-cell analysis
  • Stem cell research
  • Tips and troubleshooting
  • Women in STEM
  • That's Good Support!
  • About our blog
  • That's Good Science!
  • SMART-Seq Pro Biomarker Discovery Contest
  • DNA extraction educational activity
  • That's Good Science Podcast
  • Season one
  • Season two
  • Season three
  • Events
  • Biomarker discovery events
  • Calendar
  • Conferences
  • Speak with us
  • Careers
  • Company benefits
  • International Contacts by Region
  • United States and Canada
  • China
  • Japan
  • Korea
  • Europe
  • India
  • Affiliates & distributors
  • Need help?
  • Privacy request
Takara Bio
  • Products
  • Services & Support
  • Learning centers
  • APPLICATIONS
  • About
  • Contact Us