Terra PCR Direct Polymerase Mix and kits
Terra PCR Direct mixes and kits save time and money by allowing you to skip DNA extraction and purification, and go directly to PCR amplification.
The polymerase mix comes preblended with a monoclonal antibody for automatic hot-start PCR, and allows you to easily amplify DNA targets up to 2 kb, including difficult templates (even when the GC content is greater than 70%). We offer five different Terra PCR Direct kits formulated for various sample types and applications:
Terra PCR Direct mixes and kits save time and money by allowing you to skip DNA extraction and purification, and go directly to PCR amplification.
The polymerase mix comes preblended with a monoclonal antibody for automatic hot-start PCR, and allows you to easily amplify DNA targets up to 2 kb, including difficult templates (even when the GC content is greater than 70%). We offer five different Terra PCR Direct kits formulated for various sample types and applications:
- Polymerase mix: This kit fits most applications, and has been improved with the inclusion of Proteinase K to enhance gel detection of the PCR product.
- Red dye premix: This kit simplifies PCR setup to a single tube. It also includes a specially formulated loading dye that separates during gel electrophoresis.
- Genotyping kit: This kit includes an extraction buffer and separate loading dye, to handle the broadest range of samples and provide the most robust performance.
- Card & FFPE kits: These kits are designed to process samples preserved on Whatman FTA cards or using FFPE fixation.
Use our Terra kit and sample type selection guide to choose the right kit for your application.
Terra PCR Direct contains a novel DNA polymerase that lets you amplify directly from animal and plant tissues, as well as from crude DNA extracts. This highly sensitive DNA polymerase only requires a small amount of DNA template, thereby saving you precious samples.
Note: Proteinase K is now included with the Terra PCR Direct Polymerase Mix and the Terra PCR Direct Red Dye Premix to enhance gel detection.
Overview
- Skip sample prep
- Amplify directly from animal or plant tissues, or from crude extracts
- Easily amplify GC-rich targets
- Room-temperature setup with automatic hot start
- Specialized kits including a convenient premix, an all-in-one kit that includes extraction buffer and loading dye, and FFPE and Whatman FTA sample processing formulations
More Information
Applications
- Genotyping
- Direct PCR from crude extracts, tissues, blood, FFPE samples and many more
Bulk, custom, and OEM information
If you are interested in bulk purchasing, custom packaging, custom formulations (including glycerol-free and high concentration), or partnership opportunities, please contact Corporate Development at bd_oem@takarabio.com to discuss your needs or visit our OEM page to submit an inquiry.
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Powered by BiozTech note
Amplifying a gene of interest from human nail DNA
Terra PCR Direct Polymerase Mix allows for direct amplification from a tissue source or crude extracts, without the need for DNA purification.
Tech note
Amplifying a gene of interest from raw and processed meat samples
Terra PCR Direct polymerase demonstrates an exceptional ability to provide consistently strong amplification of a target gene directly from raw or processed meat.
See what our customers are saying about Terra PCR Direct Polymerase Mix!
"We are impressed with product. Easy to use. Helps to save time. Satisfied with results."
—Nataliia Bogdanova, UT HEALTH SCIENCE CENTER HOUSTON
"Great experience!! It was easy to use and great yield. The price is well below comparable kits. This is a great incentive to continue to use this product besides the high quality of the result."
—Katrina Owens, UNIV OF MARYLAND BALTIMORE
"This premix is very convenient. It was able to amplify the genes we are looking for within mouse tail DNA."
—Dakim Gaines, CANCER INSTITUTE OF NJ
Find answers to your PCR questions
Primer design
Frequently asked questions about primer design for successful PCR.
Optimization
Frequently asked questions about PCR optimization.
Troubleshooting
Frequently asked questions about troubleshooting your PCR problems.
Applications and conditions
Frequently asked questions about general and specific applications for PCR and which polymerases to choose.
Shipping, storage, and handling
Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.
Avoid DNA contamination in PCR
There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2022 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.
