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Terra PCR Direct Polymerase Mix and kits

Terra PCR Direct

Terra PCR Direct mixes and kits save time and money by allowing you to skip DNA extraction and purification, and go directly to PCR amplification.

The polymerase mix comes preblended with a monoclonal antibody for automatic hot-start PCR, and allows you to easily amplify DNA targets up to 2 kb, including difficult templates (even when the GC content is greater than 70%). We offer five different Terra PCR Direct kits formulated for various sample types and applications:

Terra PCR Direct mixes and kits save time and money by allowing you to skip DNA extraction and purification, and go directly to PCR amplification.

The polymerase mix comes preblended with a monoclonal antibody for automatic hot-start PCR, and allows you to easily amplify DNA targets up to 2 kb, including difficult templates (even when the GC content is greater than 70%). We offer five different Terra PCR Direct kits formulated for various sample types and applications:

  • Polymerase mix: This kit fits most applications, and has been improved with the inclusion of Proteinase K to enhance gel detection of the PCR product.
  • Red dye premix: This kit simplifies PCR setup to a single tube. It also includes a specially formulated loading dye that separates during gel electrophoresis.
  • Genotyping kit: This kit includes an extraction buffer and separate loading dye, to handle the broadest range of samples and provide the most robust performance.
  • Card & FFPE kits: These kits are designed to process samples preserved on Whatman FTA cards or using FFPE fixation.

Use our PCR selection guide to choose the right kit for your application.

Terra PCR Direct contains a novel DNA polymerase that lets you amplify directly from animal and plant tissues, as well as from crude DNA extracts. This highly sensitive DNA polymerase only requires a small amount of DNA template, thereby saving you precious samples.

Note: Proteinase K is now included with the Terra PCR Direct Polymerase Mix and the Terra PCR Direct Red Dye Premix to enhance gel detection.

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Cat. # Product Size Price License Quantity Details
639270 Terra™ Direct PCR Polymerase Mix 200 Rxns USD $203.00

A hot-start polymerase mix that is developed for direct PCR amplification from tissue samples, crude extracts, and dirty templates. It is ideal for amplifying short DNA targets (up to 2 kb), regardless of GC content or template purity. The polymerase mix is supplied with separate tubes of buffer (with Mg2+ and dNTPs) and Proteinase K. The inclusion of Proteinase K enhances gel detection of the PCR products.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data Resources

Back

639270: Terra PCR Direct Polymerase Mix

639270: Terra PCR Direct Polymerase Mix

Back

Terra PCR Direct was used to amplify the cyclin D2 gene (Ccnd2, 0.5 kb; Lane 1) and the transferrin receptor gene (TfrC 2 kb; Lane 2) from 1 μl of mouse blood treated with either EDTA or heparin

Terra PCR Direct was used to amplify the cyclin D2 gene (Ccnd2, 0.5 kb; Lane 1) and the transferrin receptor gene (TfrC 2 kb; Lane 2) from 1 μl of mouse blood treated with either EDTA or heparin
Panel A. Terra PCR Direct was used to amplify the cyclin D2 gene (Ccnd2, 0.5 kb; Lane 1) and the transferrin receptor gene (TfrC, 2 kb; Lane 2) from 1 μl of mouse blood treated with either EDTA or heparin. Panel B. Terra PCR Direct was used to amplify the mouse Ywhaz1 gene (1 kb) directly from either a 1 mm tail or 1.5 mm2 ear biopsy. A 4 μl aliquot of each sample was mixed with gel loading buffer that either lacked or contained proteinase K (Lanes 1 and 2, respectively). The PCR products treated with proteinase K ran as expected, whereas those without proteinase K treatment got stuck in the wells. Panel C. Terra PCR Direct was used to amplify the cytochrome c oxidase gene (cox1; 0.5 kb) directly from 0.5 mm (Lane 1) and 1.2 mm (Lane 2) tomato or spinach leaf cuttings (made using hole punches).
639271 Terra™ Direct PCR Polymerase Mix 800 Rxns USD $676.00

A hot-start polymerase mix that is developed for direct PCR amplification from tissue samples, crude extracts, and dirty templates. It is ideal for amplifying short DNA targets (up to 2 kb), regardless of GC content or template purity. The polymerase mix is supplied with separate tubes of buffer (with Mg2+ and dNTPs) and Proteinase K. The inclusion of Proteinase K enhances gel detection of the PCR products.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data Resources

Back

Terra PCR Direct was used to amplify the cyclin D2 gene (Ccnd2, 0.5 kb; Lane 1) and the transferrin receptor gene (TfrC 2 kb; Lane 2) from 1 μl of mouse blood treated with either EDTA or heparin

Terra PCR Direct was used to amplify the cyclin D2 gene (Ccnd2, 0.5 kb; Lane 1) and the transferrin receptor gene (TfrC 2 kb; Lane 2) from 1 μl of mouse blood treated with either EDTA or heparin
Panel A. Terra PCR Direct was used to amplify the cyclin D2 gene (Ccnd2, 0.5 kb; Lane 1) and the transferrin receptor gene (TfrC, 2 kb; Lane 2) from 1 μl of mouse blood treated with either EDTA or heparin. Panel B. Terra PCR Direct was used to amplify the mouse Ywhaz1 gene (1 kb) directly from either a 1 mm tail or 1.5 mm2 ear biopsy. A 4 μl aliquot of each sample was mixed with gel loading buffer that either lacked or contained proteinase K (Lanes 1 and 2, respectively). The PCR products treated with proteinase K ran as expected, whereas those without proteinase K treatment got stuck in the wells. Panel C. Terra PCR Direct was used to amplify the cytochrome c oxidase gene (cox1; 0.5 kb) directly from 0.5 mm (Lane 1) and 1.2 mm (Lane 2) tomato or spinach leaf cuttings (made using hole punches).

Back

639271: Terra PCR Direct Polymerase Mix

639271: Terra PCR Direct Polymerase Mix
639286 Terra™ Direct PCR Red Dye Premix 200 Rxns USD $231.00

A red dye-added 2X PCR master mix that contains everything necessary to amplify DNA directly from animal and plant tissue. The mix contains Terra PCR Direct Polymerase, a highly sensitive enzyme that allows amplification of targets from small amounts of template. In addition, the enzyme readily amplifies short DNA targets (up to 2 kb), regardless of GC content or template purity. A tube of Proteinase K is included to enhance gel detection of the PCR products.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data Resources

Back

Terra PCR Direct was used to amplify the cyclin D2 gene (Ccnd2, 0.5 kb; Lane 1) and the transferrin receptor gene (TfrC 2 kb; Lane 2) from 1 μl of mouse blood treated with either EDTA or heparin

Terra PCR Direct was used to amplify the cyclin D2 gene (Ccnd2, 0.5 kb; Lane 1) and the transferrin receptor gene (TfrC 2 kb; Lane 2) from 1 μl of mouse blood treated with either EDTA or heparin
Panel A. Terra PCR Direct was used to amplify the cyclin D2 gene (Ccnd2, 0.5 kb; Lane 1) and the transferrin receptor gene (TfrC, 2 kb; Lane 2) from 1 μl of mouse blood treated with either EDTA or heparin. Panel B. Terra PCR Direct was used to amplify the mouse Ywhaz1 gene (1 kb) directly from either a 1 mm tail or 1.5 mm2 ear biopsy. A 4 μl aliquot of each sample was mixed with gel loading buffer that either lacked or contained proteinase K (Lanes 1 and 2, respectively). The PCR products treated with proteinase K ran as expected, whereas those without proteinase K treatment got stuck in the wells. Panel C. Terra PCR Direct was used to amplify the cytochrome c oxidase gene (cox1; 0.5 kb) directly from 0.5 mm (Lane 1) and 1.2 mm (Lane 2) tomato or spinach leaf cuttings (made using hole punches).

Back

639286: Terra PCR Direct Red Dye Premix

639286: Terra PCR Direct Red Dye Premix
639285 Terra™ Direct PCR Genotyping Kit 200 Rxns USD $456.00

This Genotyping Kit contains everything needed to genotype directly from animal tissue such as mouse tails. The Kit is based on the hot-start Terra PCR Direct DNA Polymerase Mix, a highly sensitive enzyme that allows amplification of targets from small amounts of template. In addition, the enzyme readily amplifies short DNA targets (up to 2 kb), regardless of GC content or template purity. Tissue Extract Buffer and PCR buffer (with Mg2+ and dNTPs) are included in the kit. A tube of Proteinase K is provided to enhance gel detection of the PCR products.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data Resources

Back

Terra PCR Direct was used to amplify the cyclin D2 gene (Ccnd2, 0.5 kb; Lane 1) and the transferrin receptor gene (TfrC 2 kb; Lane 2) from 1 μl of mouse blood treated with either EDTA or heparin

Terra PCR Direct was used to amplify the cyclin D2 gene (Ccnd2, 0.5 kb; Lane 1) and the transferrin receptor gene (TfrC 2 kb; Lane 2) from 1 μl of mouse blood treated with either EDTA or heparin
Panel A. Terra PCR Direct was used to amplify the cyclin D2 gene (Ccnd2, 0.5 kb; Lane 1) and the transferrin receptor gene (TfrC, 2 kb; Lane 2) from 1 μl of mouse blood treated with either EDTA or heparin. Panel B. Terra PCR Direct was used to amplify the mouse Ywhaz1 gene (1 kb) directly from either a 1 mm tail or 1.5 mm2 ear biopsy. A 4 μl aliquot of each sample was mixed with gel loading buffer that either lacked or contained proteinase K (Lanes 1 and 2, respectively). The PCR products treated with proteinase K ran as expected, whereas those without proteinase K treatment got stuck in the wells. Panel C. Terra PCR Direct was used to amplify the cytochrome c oxidase gene (cox1; 0.5 kb) directly from 0.5 mm (Lane 1) and 1.2 mm (Lane 2) tomato or spinach leaf cuttings (made using hole punches).

Back

639285: Terra PCR Direct Genotyping Kit

639285: Terra PCR Direct Genotyping Kit

Primer design FAQs Primer design FAQs
Optimization FAQs Optimization FAQs
Troubleshooting FAQs Troubleshooting FAQs

Overview

  • Skip sample prep
  • Amplify directly from animal or plant tissues, or from crude extracts 
  • Easily amplify GC-rich targets
  • Room-temperature setup with automatic hot start
  • Specialized kits including a convenient premix, an all-in-one kit that includes extraction buffer and loading dye, and FFPE and Whatman FTA sample processing formulations

More Information

Applications

  • Genotyping
  • Direct PCR from crude extracts, tissues, blood, FFPE samples and many more

Bulk, custom, and OEM information

If you are interested in bulk purchasing, custom packaging, custom formulations (including glycerol-free and high concentration), or partnership opportunities, please contact us at oem@takarabio.com to discuss your needs or visit our OEM page to submit an inquiry.

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


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Tech note

Amplifying a gene of interest from human nail DNA

Terra PCR Direct Polymerase Mix allows for direct amplification from a tissue source or crude extracts, without the need for DNA purification.

Learn more


Tech note

Amplifying a gene of interest from raw and processed meat samples

Terra PCR Direct polymerase demonstrates an exceptional ability to provide consistently strong amplification of a target gene directly from raw or processed meat.

Learn more


pcr success PCR learning center
Citations Citations: Terra Direct

See what our customers are saying about Terra PCR Direct Polymerase Mix!

"We are impressed with product. Easy to use. Helps to save time. Satisfied with results."
—Nataliia Bogdanova, UT HEALTH SCIENCE CENTER HOUSTON
"Great experience!! It was easy to use and great yield. The price is well below comparable kits. This is a great incentive to continue to use this product besides the high quality of the result."
—Katrina Owens, UNIV OF MARYLAND BALTIMORE
"This premix is very convenient. It was able to amplify the genes we are looking for within mouse tail DNA."
—Dakim Gaines, CANCER INSTITUTE OF NJ

Find answers to your PCR questions

Primer design

Frequently asked questions about primer design for successful PCR.

Optimization

Frequently asked questions about PCR optimization.

Troubleshooting

Frequently asked questions about troubleshooting your PCR problems.

Applications and conditions

Frequently asked questions about general and specific applications for PCR and which polymerases to choose.

Shipping, storage, and handling

Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.

Avoid DNA contamination in PCR

There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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