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  • ‹ Back to Genomic DNA purification
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Product overview

Magnetic-bead-based isolation of viral RNA/DNA and bacterial DNA from fluids

The NucleoMag Pathogen kit is designed for the isolation of viral RNA/DNA and bacterial DNA from cell-free bodily fluids such as serum or plasma, blood, homogenized tissue sample suspensions, stool sample suspensions, and swab washes. The procedure is based on the reversible adsorption of nucleic acids to paramagnetic beads. Sample lysis is achieved by incubation with a lysis buffer containing chaotropic ions and supplemented by Proteinase K digestion. Nucleic acids are bound to the paramagnetic beads via addition of NucleoMag beads and binding buffer to the lysate. After magnetic separation with the NucleoMag Separator, the paramagnetic beads are washed with our specialized Wash Buffer to remove contaminants and salts.

  • Reagents and magnetic beads for isolation of 96 samples
  • One kit for all common clinical sample types
  • High sensitivity
  • Reliable nucleic acid isolation—suitable even for low viral titers
NucleoMag principle Procedure Example data

NucleoMag principle  

The NucleoMag family of products is based on magnetic bead technology. Nucleic acids are bound to the magnetic beads under chaotropic salt conditions, contaminants are washed away with ethanolic wash buffers, and pure RNA and DNA are eluted from the beads using elution buffer. 

  NucleoMag bind           

Figure 1. NucleoMag magnetic bead technology. NucleoMag beads and binding buffer are added to the sample (left). Beads bound to DNA/RNA are held in place in the well by the magnet, and contaminants are washed away (middle). DNA/RNA is eluted while the beads are held in place by the magnet (right).

Procedure  

NucleoMag SEP

Figure 2. Overview of NucleoMag protocol for 96 samples.

Example data  

NucleoMag SEP ct value

Figure 3. A norovirus-positive stool sample and various dilutions of the sample were used for viral RNA purification using the NucleoMag Pathogen kit. Purified viral RNA was analyzed by real-time PCR.

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Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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The NucleoMag Pathogen kit is designed for the isolation of viral RNA and DNA and bacterial DNA from cell-free body fluids such as serum or plasma, blood, homogenized tissue sample suspensions, stool sample suspensions, and swab washes. This kit provides reagents and magnetic beads for isolation of 4 x 96 samples.

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Sensitivity screening for Hepatitis B virus (HBV) and Hepatitis C virus (HCV)

Sensitivity screening for Hepatitis B virus (HBV) and Hepatitis C virus (HCV)
Sensitivity screening for Hepatitis B virus (HBV) and Hepatitis C virus (HCV). Dilution series (1:10) of human plasma (200 �l, with original virus titer as shown) were extracted in triplicate with the NucleoMag Pathogen kit and tested with the RealStar HBV PCR Kit 1.0 and the HCV RT-PCR Kit 1.0 (Altona Diagnostics). The NucleoMag Pathogen kit demonstrates a sufficient sensitivity for Hepatitis B virus (HBV) and Hepatitis C virus (HCV) detection in human plasma sample material. The internal control was extracted and recovered as expected. PCR inhibitors were not detected.

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Reliable detection of viral RNA

Reliable detection of viral RNA
Reliable detection of viral RNA. A Norovirus-positive stool sample and various dilutions of the sample were processed for viral RNA purification using the NucleoMag Pathogen kit and competitor kit "R". Purified viral RNA was analyzed by qPCR.

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Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Sensitivity screening for pathogen detection in human feces samples. Dilution series of 10-3–10-4 of feces samples were prepared for sensitivity screening using three replicates. Nucleic acid extraction was performed with the NucleoMag Pathogen kit and the competitor kit "R". PCR analysis was performed using the RIDA GENE Viral Stool Panel I (R-Biopharm) and RealStar Clostridium difficile PCR Kit 1.0 (Altona Diagnostics).

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Isolation of viral RNA and DNA and bacterial DNA from cell-free body fluids,homogenized tissue sample suspensions, stool sample suspensions, and swab washes

RNA and DNA isolation from pathogens

The NucleoMag Pathogen kit is designed for the rapid manual and automated small-scale isolation of viral RNA and DNA and bacterial DNA from cell-free body fluids such as serum or plasma, blood, homogenized tissue sample suspensions, stool sample suspensions, and swab washes.

NucleoMag Pathogen NucleoMag SEP magnetic separator

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Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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