Magnetic-bead-based isolation of viral RNA/DNA and bacterial DNA from fluids

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Nucleic acid purification

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Product overview

Magnetic-bead-based isolation of viral RNA/DNA and bacterial DNA from fluids

The NucleoMag Pathogen kit is designed for the isolation of viral RNA/DNA and bacterial DNA from cell-free bodily fluids such as serum or plasma, blood, homogenized tissue sample suspensions, stool sample suspensions, and swab washes. The procedure is based on the reversible adsorption of nucleic acids to paramagnetic beads. Sample lysis is achieved by incubation with a lysis buffer containing chaotropic ions and supplemented by Proteinase K digestion. Nucleic acids are bound to the paramagnetic beads via addition of NucleoMag beads and binding buffer to the lysate. After magnetic separation with the NucleoMag Separator, the paramagnetic beads are washed with our specialized Wash Buffer to remove contaminants and salts.

Reagents and magnetic beads for isolation of 96 samples
One kit for all common clinical sample types
High sensitivity
Reliable nucleic acid isolation—suitable even for low viral titers

NucleoMag principle Procedure Example data

NucleoMag principle

The NucleoMag family of products is based on magnetic bead technology. Nucleic acids are bound to the magnetic beads under chaotropic salt conditions, contaminants are washed away with ethanolic wash buffers, and pure RNA and DNA are eluted from the beads using elution buffer.

Procedure

Example data

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Isolation of viral RNA and DNA and bacterial DNA from cell-free body fluids,homogenized tissue sample suspensions, stool sample suspensions, and swab washes

RNA and DNA isolation from pathogens

The NucleoMag Pathogen kit is designed for the rapid manual and automated small-scale isolation of viral RNA and DNA and bacterial DNA from cell-free body fluids such as serum or plasma, blood, homogenized tissue sample suspensions, stool sample suspensions, and swab washes.

NucleoMag Pathogen NucleoMag SEP magnetic separator

Takara Bio USA, Inc.
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