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  • ‹ Back to DNA/RNA cleanup and extraction
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PRODUCT OVERVIEW

NucleoSpin RNA Clean-up

  • Complete removal of RT-PCR inhibitors
  • Time-saving procedure based on NucleoSpin RNA, without DNase digestions and homogenization steps
  • RNA cleanup from pre-purified RNA (phenol/chloroform), enzymatic reactions (e.g., amplification reactions, labeling reactions)
  • For isolation of RNA from cells and tissue with minimal DNA contamination, we recommend rDNase-containing NucleoSpin RNA kits
  • XS format available for extra-small sample sizes (see specifications in the At-a-glance table)
At-a-glance Applications Protocol overview Application data XS format

At-a-glance  

Technology Silica-membrane technology
Format Mini spin columns
Sample material ≤200 µl phenol/chloroform extract, reaction mixture, or <105 cells; <300 ul RNA sample containing <90 ug RNA (XS)
Fragment size >200 nt
Typical recovery 85–95%
A260/A280 1.9–2.1
Typical RIN (RNA integrity number) Depends on sample quality (no significant loss of RIN detected)
Elution volume 40–120 µl; 5–30 µl (XS)
Preparation time 20 min/6 preps
Binding capacity 200 µg; 110 µg (XS)

Applications  

RNA clean-up for:

  • Pre-purified RNA (e.g., Trizol)
  • Reaction mixtures
  • Amino-allyl-mRNA
  • Biotinylated RNA
  • RNA isolation from up to 105 cultured cells (whenever co-purification of some genomic DNA is acceptable, kit does not contain rDNase)
  • Typical downstream applications: enzymatic-labeling reactions, RT-PCR, DNA/RNA-based chip hybridizations

NucleoSpin RNA Clean-Up kits are recommended for the cleanup of total RNA from RNA preparations which contain unacceptable amounts of RT-PCR inhibitors, such as RNA prepared with phenol-chloroform based methods. These kits are also suitable for the isolation of RNA from small amounts of cultured cells whenever copurification of some genomic DNA is acceptable. The kits allow purification of pure RNA with an A260/A280 ratio generally exceeding 1.9 (measured in TE buffer, pH 7.5).

NucleoSpin RNA Clean-Up kits are also recommended for cleanup of in vitro-transcribed RNA and aminoallyl mRNA, as well as biotinylated and fluorescently-labeled RNA. Purified RNA is ready for use in applications such as enzymatic labeling reactions (including dye incorporation), RT-PCR, and DNA/RNA-based chip hybridizations.

The integrity of purified RNA can be examined by denaturing agarose gel electrophoresis.

Protocol overview  

RNA-containing samples are mixed with a solution containing large amounts of chaotropic ions. This solution immediately inactivates RNases—which are present in virtually all biological materials—and creates appropriate binding conditions which favor adsorption of RNA to the silica membrane. Simple washing steps remove salts, metabolites, organics like phenol, and cellular debris. Pure RNA can then be eluted with RNase-free water.

NucleoSpin RNA Clean-Up kit procedure.

Application data  

The cRNA purified with NucleoSpin RNA Clean-up Kit from Macherey-Nagel is very suitable for downstream biochip technology and detection using the products from Affymetrix, and gives very excellent results.
—Guido Krupp, Ph.D., Director and Founder, artus GmbH, Hamburg, Germany

cRNA analysis with Bioanalyzer

Amino-allyl-modified cRNA (total RNA from rat kidney) was purified using either NucleoSpin RNA Clean-Up (blue) or using a kit from competitor Q (red). 1/20 μl was analyzed by Agilent Bioanalyzer 2100 (green: RNA 6000 ladder, Thermo Fisher Scientific).

Data kindly provided by Dr. Guido Krupp, artus GmbH, Hamburg.

XS format  

Purification of high-quality RNA from reaction mixtures
RNA quality (RIN) and quantity were analyzed with an Agilent® Bioanalyzer, Agilent RNA 6000 Nano chip, and Agilent RNA 6000 Nano reagent before and after clean-up. The crude RNA solution (lanes 1 and 2) show low RNA concentrations around 85 ng/μL. Nevertheless, the RNA is not degraded and has a high RIN of about 9.4. Lanes 3 and 4 show the corresponding RNA after DNase digestion and purification with NucleoSpin RNA Clean-up XS. RNA quality is not affected by the whole procedure, showing similar RIN and high recovery of about 95% in a total volume of 20 μL.

Related Products

Cat. # Product Size License Quantity Details
740948.250 NucleoSpin® RNA Clean-up 250 Preps USD $1060.00

NucleoSpin RNA Clean-up (250) 250 preps for clean-up of total RNA from reaction mixtures - NucleoSpin RNA Columns, Collection Tubes, buffers

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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The NucleoSpin RNA Clean-up procedure

The NucleoSpin RNA Clean-up procedure
The NucleoSpin RNA Clean-up procedure.

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740948.250: NucleoSpin RNA Clean-up

740948.250: NucleoSpin RNA Clean-up
740948.50 NucleoSpin® RNA Clean-up 50 Preps USD $223.00

NucleoSpin RNA Clean-up (50) 50 preps for clean-up of total RNA from reaction mixtures - NucleoSpin RNA Columns, Collection Tubes, buffers

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740903.250 NucleoSpin® RNA Clean-up XS 250 Preps USD $1465.00

NucleoSpin RNA Clean-up XS (250) 250 preps for the clean-up of total RNA - NucleoSpin RNA XS Columns, Collection Tubes, buffers

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes

NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes

NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes. Panel A. The columns are optimized to hold a very small silica membrane, yet fit inside a standard 1.5 ml microfuge tube. Panel B. The exceptionally small (2.0 mm) diameter of the NucleoSpinXS membrane (Column MN) allows efficient elution in only 5–30 μl. It provides a significant advantage over the small-sample column offered by Competitor Q (Column Q; 4.8 mm diameter), which requires an elution volume of at least 10–30 μl.

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740903.250: NucleoSpin RNA Clean-up XS

740903.250: NucleoSpin RNA Clean-up XS
740903.50 NucleoSpin® RNA Clean-up XS 50 Preps USD $332.00

NucleoSpin RNA Clean-up XS (50) 50 preps for the clean-up of total RNA - NucleoSpin RNA XS Columns, Collection Tubes, buffers

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes

NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes

NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes. Panel A. The columns are optimized to hold a very small silica membrane, yet fit inside a standard 1.5 ml microfuge tube. Panel B. The exceptionally small (2.0 mm) diameter of the NucleoSpinXS membrane (Column MN) allows efficient elution in only 5–30 μl. It provides a significant advantage over the small-sample column offered by Competitor Q (Column Q; 4.8 mm diameter), which requires an elution volume of at least 10–30 μl.

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740903.50: NucleoSpin RNA Clean-up XS

740903.50: NucleoSpin RNA Clean-up XS
740903.10 NucleoSpin® RNA Clean-up XS 10 Preps USD $76.00

NucleoSpin RNA Clean-up XS (10) 10 preps for the clean-up of total RNA - NucleoSpin RNA XS Columns, Collection Tubes, buffers

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes

NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes

NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes. Panel A. The columns are optimized to hold a very small silica membrane, yet fit inside a standard 1.5 ml microfuge tube. Panel B. The exceptionally small (2.0 mm) diameter of the NucleoSpinXS membrane (Column MN) allows efficient elution in only 5–30 μl. It provides a significant advantage over the small-sample column offered by Competitor Q (Column Q; 4.8 mm diameter), which requires an elution volume of at least 10–30 μl.

Back

740903.10: NucleoSpin RNA Clean-up XS

740903.10: NucleoSpin RNA Clean-up XS
740963 rDNase Set Each USD $90.00

The rDNase Set is intended for performing RNA minipreps using NucleoSpin RNA kits. Cat. # 740963 consists of one vial of RNase-free recombinant DNase (lyophilized), plus 7 ml Reaction Buffer.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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