|Format||Highly reactive superparamagnetic beads|
|Processing||Manual or automated|
|Input material||Reaction mixtures from common NGS library preparation kits|
|Amount of input material||17.5 pg–5 µg|
|Input volume||50–150 µl|
|Elution volume||10–100 µl|
NucleoMag NGS Clean-up and Size Select
The NucleoMag NGS Clean-up and Size Select kit uses magnetic-bead technology to enable efficient cleanup of enzymatic reactions and tunable size selection of DNA fragments for NGS library preparation. The kit makes it possible to selectively target fragments of a given size within a range of ~150–800 bp by varying bead-to-sample volume ratios and performing single- or double-sided size selection (Figure 1). The beads can easily be used in existing NGS library preparation protocols since the recommended dilution ratios are similar to those used with other magnetic bead-based methods.
The NucleoMag NGS Clean-up and Size Select procedure is based on reversible adsorption of DNA fragments to paramagnetic beads. The purified DNA fragments, which are eluted in low-salt buffer or water after an ethanol wash, are free of contaminating nucleotides, primers, adapters, adapter dimers, enzymes, buffer additives, or salts, and can be used directly in downstream applications.
The recovery rates for different fragment sizes vary depending on the bead-to-sample volume ratios employed (Figure 2), and varying volume ratios can be applied in series for double-sided size selection (Figure 3). The NucleoMag NGS Clean-up and Size Select kit has been shown to yield comparable fragment size distributions (Figure 4) and sequencing data (Figure 5) to size-selection beads from other providers.
The NucleoMag NGS Clean-up and Size Select kit offers the following benefits:
- Processing of inputs as low as 17.5 pg and input volumes ranging from 50–150 µl
- Tunable selection of DNA fragments ranging in size from 150–800 bp
- Scalable, automation-friendly magnetic-bead technology
- Comparable performance and dilution ratios to beads from other providers (e.g., AMPure XP)
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