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Overviews RNA purification from soil
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Product overview

High-throughput purification of DNA from soil: NucleoSpin 8/96 Soil

Soil samples contain different amounts of organic matter and inorganic material (humic substances or polysaccharides) and exhibit different pHs, all of which can hinder the consistency of DNA extraction from these sample types. The NucleoSpin 8/96 Soil kits overcome these obstacles through the use of highly optimized lysis conditions and mechanical disruption. Using these kits with 500 mg of soil as a starting material typically yields 2–10 µg of eluted genomic DNA that is ready to use for downstream applications such as PCR, qPCR, restriction analysis, etc.

  • Alternative lysis buffers and additional Enhancer SX enable optimization of the protocol to maximize yields from soil samples of different compositions
  • MN Bead Tubes Type A (ceramic beads) facilitate more efficient lysis of soil microorganisms and reduce risk of cross-contamination
  • NucleoSpin Inhibitor Removal Strips/Plate allow for elimination of all PCR inhibitors, such as humic substances

At-a-glance  

NucleoSpin 8 Soil NucleoSpin 96 Soil
Technology Silica-membrane technology Silica-membrane technology
Format 8-well strip 96-well plates
Processing Manual or automated, vacuum or centrifugation Manual or automated, vacuum or centrifugation
Starting material <500 mg soil, sludge, or sediment <500 mg soil, sludge, or sediment
Fragment size 50 bp–approx. 50 kb 50 bp–approx. 50 kb
Typical yield 2–10 µg (500 mg soil) 2–10 µg (500 mg soil)
A260/A280 1.6–1.8 1.6–1.8
Elution volume 100–200 µl 100–200 µl
Preparation time 150 min/6 strips 150 min/plate
Binding capacity 50 µg 50 µg

Application data  

DNA isolation from soil

DNA isolation from Alaska Gelisol (active)DNA isolation from Alaska Gelisol (permafrost)

Figure 1. DNA isolation from Alaskan gelisol. Bacterial genome copy numbers were determined for DNA that was extracted from 250-mg soil samples derived from active (Panel A) and permafrost (Panel B) layers of Alaska gelisol using the NucleoSpin kit in comparison with a competitor kit from Mo Bio. Far more bacterial DNA was extracted with the NucleoSpin kit using the combination of Buffer S1 and Buffer SX than with the competitor kit. (Data provided by customers.)

DNA isolation from peat

DNA isolation from peat (250 mg)DNA isolation from peat (500 mg)

Figure 2. DNA isolation from spruce peat. Bacterial genome copy numbers were determined for DNA that was extracted from 250-mg (Panel A) and 500-mg (Panel B) samples of spruce peat using the NucleoSpin kit in comparison with a competitor kit from Mo Bio. Far more bacterial DNA was extracted with the NucleoSpin kit using Buffer S1 (with or without Buffer SX) than with the competitor kit. (Data provided by customers.)

Automated DNA isolation from soil

NucleoSpin 8/96 Soil yield and purityNucleoSpin 8/96 Soil gel analysisNucleoSpin 8/96 Soil PCR performance

Figure 3. Automated DNA extraction from soil on the epMotion 5075 VAC. DNA was extracted from 500-mg inputs of soil on the epMotion 5075 liquid-handling system using each possible lysis buffer combination provided with the NucleoSpin 96 Soil kit. For this sample type, the highest yield of high molecular weight DNA was observed for the SL2 + SX Buffer combination (Panels A and B). No PCR inhibition was observed when 2-µl volumes of eluate from each preparation were used as templates (Panel C).

Protocol  

NucleoSpin 8/96 Soil procedure

Figure 4. Protocol overviews for NucleoSpin 8 Soil and NucleoSpin 96 Soil. NucleoSpin 8 Soil allows for processing of 48 samples (6 strips) in approximately 150 min, whereas NucleoSpin 96 Soil enables processing of 96 samples (1 plate) in the same amount of time. Both kits can be used with vacuum- or centrifuge-based processing and are compatible with manual or automated workflows.

Required hardware  

Centrifugation: Centrifuge-based processing of NucleoSpin 8/96 Soil kits requires a microtiter plate centrifuge which is able to accommodate the NucleoSpin Soil Strips/Plate stacked on a round- or square-well block or rack with tube strips. The centrifuge must be able to reach an acceleration of 5,600–6,000g (bucket height: 85 mm). Centrifuge-based processing of 8-well strips requires the reusable Starter Set C, which contains Column Holders C, NucleoSpin Dummy Strips, MN Square-well Blocks, and Tube Strips.

Vacuum processing: The NucleoSpin 8/96 Soil kits also allow for manual processing with the NucleoVac 96 Vacuum Manifold. Additionally, a suitable centrifuge for sample preparation steps may be required. Vacuum processing of 8-well strips and automation on laboratory platforms with standard 96-well plate vacuum chambers require the reusable Starter Set A, which contains Column Holders A and Dummy Strips.

Related products

Cat. # Product Size License Quantity Details
740779 NucleoSpin® 8 Soil 12 x 8 Preps USD $407.00

The NucleoSpin 8 Soil kit is designed for the isolation of high molecular weight genomic DNA from microorganisms such as Gram-positive and Gram-negative bacteria, archaea, fungi, and algae in soil, sludge, and sediment samples. The kit is also suitable for stool samples. It provides two special lysis buffers, Buffer SL1 and Buffer SL2 , which can be combined with the chemical additive Enhancer SX to guarantee the highest possible yields with excellent purity for all types of sample material. Efficient mechanical lysis of the sample material is achieved by bead beating using MN Bead Tubes Type A (Cat. # 740786.50).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740787.2 NucleoSpin® 96 Soil 2 x 96 Preps USD $765.00

The NucleoSpin 96 Soil kit is designed for the isolation of high molecular weight genomic DNA from microorganisms such as Gram-positive and Gram-negative bacteria, archaea, fungi, and algae in soil, sludge, and sediment samples. The kit is also suitable for stool samples. It provides two special lysis buffers, Buffer SL1 and Buffer SL2 , which can be combined with the chemical additive Enhancer SX to guarantee the highest possible yields with excellent purity for all types of sample material. Efficient mechanical lysis of the sample material is achieved by bead beating using MN Bead Tubes Type A (Cat. # 740786.50).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740787.4 NucleoSpin® 96 Soil 4 x 96 Preps USD $1320.00

The NucleoSpin 96 Soil kit is designed for the isolation of high molecular weight genomic DNA from microorganisms such as Gram-positive and Gram-negative bacteria, archaea, fungi, and algae in soil, sludge, and sediment samples. It is also suitable for stool samples. The kit provides two special lysis buffers, Buffer SL1 and Buffer SL2, which can be combined with the chemical additive Enhancer SX to guarantee the highest possible yields with excellent purity for various sample materials. Efficient mechanical lysis of the sample material is achieved by bead beating using MN Bead Tubes Type A (Cat. # 740786.50).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740681 NucleoVac™ 96 Vacuum Manifold Each USD $665.00

NucleoVac 96 Vacuum Manifold vacuum manifold for use of NucleoSpin 8/96 kits under vacuum - manifold base, manifold lid, waste containers, spacer set

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740641 NucleoVac™ Vacuum Regulator Each USD $194.00

NucleoVac 96 Vacuum Regulator for use with NucleoVac 96 Vacuum Manifold, for controlling of vacuum

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Image Data
740682 Starter Set A Each USD $327.00

Starter Set A for processing NucleoSpin 8-well strips under vacuum on the NucleoVac 96 Vacuum Manifold or similar manifolds - 2 Column Holders A, 12 NucleoSpin Dummy Strips

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data
740684 Starter Set C Each USD $461.00

Starter Set C for processing NucleoSpin 8-well strips under centrifugation - 2 Column Holders C, 2 Racks of Tube Strips, 2 MN Square-well Blocks, Rack of Tube Strips

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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