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  • In-Fusion Snap Assembly
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In-Fusion Snap Assembly EcoDry for seamless DNA cloning in a lyophilized format

In-Fusion Snap Assembly EcoDry products feature our In-Fusion Snap Assembly master mix in a lyophilized, ready-to-use format that can be stored at room temperature. The cloning reagent is prealiquoted and dried down in tubes or well-plates that are covered with an aluminum seal. The room temp stability makes this convenient cloning enzyme format ideal for high-throughput cloning with robotic liquid handlers.

In-Fusion Snap Assembly EcoDry products feature our In-Fusion Snap Assembly master mix in a lyophilized, ready-to-use format that can be stored at room temperature. The cloning reagent is prealiquoted and dried down in tubes or well-plates that are covered with an aluminum seal. The room temp stability makes this convenient cloning enzyme format ideal for high-throughput cloning with robotic liquid handlers. Whether at the bench or with automation platforms, use of pre-aliquoted reagents means minimal handling—eliminating chances of contamination, pipetting errors, or missing reagents. Just like its liquid counterpart, In-Fusion Snap Assembly EcoDry allows you to clone any PCR fragment into any linearized vector in a single step without restriction digestion of the PCR fragment, ligation, or blunt-end polishing.

The ability to clone directly into any vector at any restriction site eliminates the need for further subcloning or manipulation, dramatically simplifying the cloning workflow and enabling transition to high-throughput applications. In-Fusion Snap Assembly EcoDry master mix fuses PCR-generated sequences and linearized vectors efficiently and precisely, utilizing a 15-bp overlap at their ends. This 15-bp overlap can be engineered by designing custom primers for amplification of the desired sequences using our free primer design tool.

In-Fusion Cloning technology is based on the removal of nucleotides from the 3' end of the linear DNA strands. This allows complementary base pairs between two fragments of DNA to anneal, leading to fragment joining. This method can be used to clone single or multiple fragments into a single vector without subcloning. Additional applications include directional cDNA cloning, mutagenesis, gene synthesis, high-throughput cloning, as well as the addition of adaptors, linkers, and protein tags.

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Cat. # Product Size Price License Quantity Details
638954 In-Fusion® Snap Assembly EcoDry™ Master Mix 8 Rxns USD $190.00

The In-Fusion Snap Assembly EcoDry Master Mix enables high-efficiency, high-fidelity, directional cloning of one or more PCR fragments into any vector. The cornerstone of In-Fusion Cloning technology is Takara Bio’s proprietary In-Fusion master mix, which fuses DNA fragments (e.g., PCR-generated sequences and linearized vectors) efficiently and precisely by recognizing a 15-bp overlap at their ends. This 15-bp overlap can be engineered by designing primers for amplification of the desired sequences. The In-Fusion Snap Assembly Master Mix offers high cloning efficiency, especially for longer fragments, short oligonucleotides, and multiple fragments. It is provided in a convenient, lyophilized (dry) format.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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638954: In-Fusion Snap Assembly EcoDry Master Mix

638954: In-Fusion Snap Assembly EcoDry Master Mix

Back

The In-Fusion cloning protocol

The In-Fusion cloning protocol

The In-Fusion cloning protocol.

638955 In-Fusion® Snap Assembly EcoDry™ Master Mix 32 Rxns USD $568.00

The In-Fusion Snap Assembly EcoDry Master Mix enables high-efficiency, high-fidelity, directional cloning of one or more PCR fragments into any vector. The cornerstone of In-Fusion Cloning technology is Takara Bio’s proprietary In-Fusion master mix, which fuses DNA fragments (e.g., PCR-generated sequences and linearized vectors) efficiently and precisely by recognizing a 15-bp overlap at their ends. This 15-bp overlap can be engineered by designing primers for amplification of the desired sequences. The In-Fusion Snap Assembly Master Mix offers high cloning efficiency, especially for longer fragments, short oligonucleotides, and multiple fragments. It is provided in a convenient, lyophilized (dry) format.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

Back

The In-Fusion cloning protocol

The In-Fusion cloning protocol

The In-Fusion cloning protocol.

Back

638955: In-Fusion Snap Assembly EcoDry Master Mix

638955: In-Fusion Snap Assembly EcoDry Master Mix
638956 In-Fusion® Snap Assembly EcoDry™ Master Mix 96 Rxns USD $1270.00

The In-Fusion Snap Assembly EcoDry Master Mix enables high-efficiency, high-fidelity, directional cloning of one or more PCR fragments into any vector. The cornerstone of In-Fusion Cloning technology is Takara Bio’s proprietary In-Fusion master mix, which fuses DNA fragments (e.g., PCR-generated sequences and linearized vectors) efficiently and precisely by recognizing a 15-bp overlap at their ends. This 15-bp overlap can be engineered by designing primers for amplification of the desired sequences. The In-Fusion Snap Assembly Master Mix offers high cloning efficiency, especially for longer fragments, short oligonucleotides, and multiple fragments. It is provided in a convenient, lyophilized (dry) format.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

Back

The In-Fusion cloning protocol

The In-Fusion cloning protocol

The In-Fusion cloning protocol.

Back

638956: In-Fusion Snap Assembly EcoDry Master Mix

638956: In-Fusion Snap Assembly EcoDry Master Mix
638953 In-Fusion® Snap Assembly EcoDry™ Master Mix with Competent Cells 96 Rxns USD $1633.00

The In-Fusion Snap Assembly EcoDry Master Mix with Competent Cells enables high-efficiency, high-fidelity, directional cloning of one or more PCR fragments into any vector. The cornerstone of In-Fusion Cloning technology is Takara Bio’s proprietary In-Fusion master mix, which fuses DNA fragments (e.g., PCR-generated sequences and linearized vectors) efficiently and precisely by recognizing a 15-bp overlap at their ends. This 15-bp overlap can be engineered by designing primers for amplification of the desired sequences. The In-Fusion Snap Assembly Master Mix offers high cloning efficiency, especially for longer fragments, short oligonucleotides, and multiple fragments. It is provided in a convenient, lyophilized (dry) format. In addition to the cloning kit, this package includes Stellar™ Competent Cells, an E. coli HST08 strain that provides high transformation efficiency paired with blue-white screening capability when used with pUC plasmid vectors.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

Back

The In-Fusion cloning protocol

The In-Fusion cloning protocol

The In-Fusion cloning protocol.

Back

638953: In-Fusion Snap Assembly EcoDry Master Mix with Competent Cells

638953: In-Fusion Snap Assembly EcoDry Master Mix with Competent Cells

Overview

Discover all the benefits of In-Fusion Cloning, in a dried-down format:

  • Ease of use and consistency—Lyophilized, pre-aliquoted cloning mix; dried-down format eliminates handling errors and contamination, facilitating high-throughput workflows
  • Highly efficient—Over 95% efficiency demonstrated with a broad range of fragment sizes from 0.5 kb to 15 kb
  • Sub-cloning is unnecessary—Clone any insert, into any locus, in any vector, with just one reaction
  • Seamless construction—Final constructs have no superfluous base pairs (as is often the case with restriction digest or TA cloning)
  • Versatility and flexibility for your applications—Clone single or multiple DNA fragments simultaneously, or perform site-directed mutagenesis in a single reaction

More Information

Applications

  • High-throughput cloning (see full range of reagent options on our high-throughput cloning page)
  • Directional cDNA cloning
  • Multiple fragment cloning
  • Site-directed mutagenesis
  • Gene synthesis
  • Adding adaptors, linkers, and protein tags
  • Cloning using modular parts

Choose the right In-Fusion Cloning system for your needs

View our selection guide.

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


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Competent cells Stellar Competent Cells overview
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Selection guides In-Fusion Cloning selection guide

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