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Stellar Competent Cells arranged in a 96-well plate for high-throughput transformation. Stellar Cells are an E. coli HST08 strain that provides high transformation efficiency. These cells can be used in a wide variety of applications: from preparation of cDNA and genomic libraries, to construction of longer-length genomic libraries, to subcloning, and even methylated DNA cloning. Stellar Competent Cells lack the gene cluster for cutting foreign methylated DNA (mrr-hsdRMS-mcrBC and mcrA), and are therefore useful for cloning methylated DNA. The cells can also be used for blue/white screening (i.e., α-complementation) when transformed with vectors containing the lacZα gene. A pUC19 vector, SOC Medium, 8-Cap Strips, and a Plate Lid are included. These competent cells are recommended for use with Takara Bio's In-Fusion PCR Cloning Kits.
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PrimeSTAR Max DNA Polymerase is a B-family polymerase blend that delivers exceptional fidelity and ultra-fast extension speed, making it ideal for applications such as cloning, mutagenesis, and high-throughput PCR. Includes a 2X premix that contains all necessary components (enzyme, buffer, dNTP mix) for assay setup. Primers and template not included.
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NucleoSpin 96 PCR Clean-up (1x96) 1x 96 preps for the purification of PCR products - NucleoSpin PCR Clean-up Binding Plate, MN Wash Plate, Elution Plate U-bottom, Self-adhering Foil, buffers
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