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Cellartis DEF-CS 500 Culture System citation list
Takara Bio has over 15 years of experience in expanding, passaging and culturing human pluripotent stem cells under the Cellartis brand. The DEF-CS 500 system is a complete solution for the easy culturing of human pluripotent stem cells in a noncolony, 2D monolayer format. Cells grown in this system maintain pluripotency and a normal karyotype while exhibiting robust and uniform growth. Single-cell passaging enables the ability to directly isolate and expand single clones, such as cells that have undergone CRISPR/Cas9 gene editing.
Read below for a citation list of studies in which DEF-CS 500 system was used in peer-reviewed basic, translational, preclinical, and biomedical research.
- Asplund, A. et al. One Standardized Differentiation Procedure Robustly Generates Homogenous Hepatocyte Cultures Displaying Metabolic Diversity from a Large Panel of Human Pluripotent Stem Cells. Stem Cell Rev. 12, 90–104 (2016).
Twenty-five different human pluripotent stem cell lines were maintained with the DEF-CS system. Pluripotency of stem cells maintained in DEF-CS was confirmed with positive stem cell markers as well as negative differentiation markers. Karyotyping of the stem cells maintained using the DEF-CS system for up to 29 passages showed no genetic aberrations. These stem cell lines were later successfully differentiated into functional hepatocytes. The DEF-CS system has been shown to be a highly robust culture system across multiple hiPSC lines and preserves pluripotency.
- Boreström, C. et al. Footprint-free human induced pluripotent stem cells from articular cartilage with redifferentiation capacity: a first step toward a clinical-grade cell source. Stem Cells Transl. Med. 3, 433–47 (2014).
Chondrocyte-derived iPSC and fibroblast-derived iPSC were maintained using DEF-CS system in monolayer before these cells were successfully differentiated down to the chondrogenic lineage. Pluripotency of iPSC maintained in DEF-CS was confirmed with immunofluorescent staining and quantitative RT-PCR.
- Norrman, K., Strömbeck, A., Semb, H. & Ståhlberg, A. Distinct gene expression signatures in human embryonic stem cells differentiated towards definitive endoderm at single-cell level. Methods 59, 59–70 (2013).
Human embryonic stem cells were maintained in the DEF-CS system before differentiated into definitive endoderm.
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