Concentrate retroviral supernatants without ultracentrifugation
Retro-X Concentrator provides a fast, simple, and highly efficient method for concentrating any retroviral stock including MMLV- or MSCV-based retroviral supernatants. In the simple protocol, you just mix your retroviral supernatant with the retrovirus concentration reagent, incubate for a short period, and spin the mixture in a standard centrifuge.
You’ll increase your retroviral titer by up to 100-fold and obtain excellent recoveries—with no ultracentrifugation.
Retro-X Concentrator provides a fast, simple, and highly efficient method for concentrating any retroviral stock including MMLV- or MSCV-based retroviral supernatants. In the simple protocol, you just mix your retroviral supernatant with the retrovirus concentration reagent, incubate for a short period, and spin the mixture in a standard centrifuge.
You’ll increase your retroviral titer by up to 100-fold and obtain excellent recoveries—with no ultracentrifugation.
Far simpler than ultracentrifugation
Compared to ultracentrifugation, the Retro-X Concentrator is more flexible in terms of scale, is easier to use than ultracentrifugation, and requires no specialized equipment.
Scalable for supernatants of any volume, any titer
The Retro-X Concentrator reagent is a 4X concentrate, so it can be added to any volume of supernatant containing any amount of virus or any starting titer. At Takara Bio, scalability has been successful for supernatant volumes up to 250 ml.
Increase titers by up to 100 fold
Using the Retro-X Concentrator protocol, we were able to increase the titer of retroviral supernatants for various envelope-pseudotyped retrovirus samples from 46-fold (amphotropic and 10A1 pseudotypes) to 100-fold (ecotropic and VSV-G), when concentrating them from 10 ml down to 100 µl.
Overview
- Simple mix-and-spin protocol
- Hassle-free and easily scaled up for large volumes
- No ultracentrifugation required
- 100X concentration
- 90% yield
- Retro-X Concentrator can also concentrate lentivirus supernatants utilizing the incubation times recommended in the Lenti-X Concentrator Protocol-At-A-Glance
More Information
Applications
- Retroviral transduction studies
- Increased multiplicity of infection (MOI)
- Rescue of low-titer supernatants
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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