Concentrate retroviral supernatants without ultracentrifugation

Retro-X Concentrator provides a fast, simple, and highly efficient method for concentrating any retroviral stock including MMLV- or MSCV-based retroviral supernatants. In the simple protocol, you just mix your retroviral supernatant with the retrovirus concentration reagent, incubate for a short period, and spin the mixture in a standard centrifuge.

You’ll increase your retroviral titer by up to 100-fold and obtain excellent recoveries—with no ultracentrifugation.

Far simpler than ultracentrifugation

Compared to ultracentrifugation, the Retro-X Concentrator is more flexible in terms of scale, is easier to use than ultracentrifugation, and requires no specialized equipment.

Scalable for supernatants of any volume, any titer

The Retro-X Concentrator reagent is a 4X concentrate, so it can be added to any volume of supernatant containing any amount of virus or any starting titer. At Takara Bio, scalability has been successful for supernatant volumes up to 250 ml.

Increase titers by up to 100 fold

Using the Retro-X Concentrator protocol, we were able to increase the titer of retroviral supernatants for various envelope-pseudotyped retrovirus samples from 46-fold (amphotropic and 10A1 pseudotypes) to 100-fold (ecotropic and VSV-G), when concentrating them from 10 ml down to 100 µl.

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Cat. #	Product	Size	Price
631456	Retro-X™ Concentrator	500 mL	USD $772.00
The Retro-X Concentrator is a complete reagent for the concentration of infectious retroviral vector particles. This reagent provides a scalable alternative to ultracentrifugation for viral particle concentration. Vector supernatants can be concentrated 10–100 fold, depending upon the volumes and envelope used. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back Simple method to concentrate retrovirus Simple method to concentrate retrovirus. Add Retro-X Concentrator reagent to clarified viral supernatant, incubate overnight at 4°C, and spin. That’s it. Back Far simpler than ultracentrifugation Far simpler than ultracentrifugation. In a side-by-side comparison of Retro-X Concentrator and ultracentrifugation, the advantages of the Retro-X method are clearly evident. Retro-X Concentrator is more flexible and easier to use than ultracentrifugation. The Retro-X Concentrator reagent is itself a 4X concentrate, so it can be added to any volume of supernatant containing any amount of virus or any starting titer. Scalability has been successful for supernatant volumes up to 250 ml. Back Retro-X Concentrator can concentrate retroviral supernatants created with all commonly used envelopes Retro-X Concentrator can concentrate retroviral supernatants created with all commonly used envelopes. A retrovirus expressing DsRed2 was packaged with different retroviral envelopes using the Retro-X Universal Packaging System (Cat. # 631530), and harvested at 72 hr posttransfection. Retro-X Concentrator was used to concentrate dualtropic (10A1), amphotropic (Ampho), ecotropic (Eco), and VSV-G (pantropic) pseudotyped retrovirus samples from 10 ml down to 100 µl (Panels A & B). Crude and concentrated viral stocks were then titrated on HT1080 or NIH 3T3 cells and analyzed via flow cytometry at 72 hr postinfection to determine the percentage of positive cells. Back Retroviral vectors available from Clontech Retroviral vectors available from Takara Bio. Not all vectors are shown. Some retroviral vectors are not sold separately. Back 631456: Retro-X Concentrator
631455	Retro-X™ Concentrator	100 mL	USD $225.00
The Retro-X Concentrator is a complete reagent for the concentration of infectious retroviral vector particles. This reagent provides a scalable alternative to ultracentrifugation for viral particle concentration. Vector supernatants can be concentrated 10–100 fold, depending upon the volumes and envelope used. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back Simple method to concentrate retrovirus Simple method to concentrate retrovirus. Add Retro-X Concentrator reagent to clarified viral supernatant, incubate overnight at 4°C, and spin. That’s it. Back Far simpler than ultracentrifugation Far simpler than ultracentrifugation. In a side-by-side comparison of Retro-X Concentrator and ultracentrifugation, the advantages of the Retro-X method are clearly evident. Retro-X Concentrator is more flexible and easier to use than ultracentrifugation. The Retro-X Concentrator reagent is itself a 4X concentrate, so it can be added to any volume of supernatant containing any amount of virus or any starting titer. Scalability has been successful for supernatant volumes up to 250 ml. Back Retro-X Concentrator can concentrate retroviral supernatants created with all commonly used envelopes Retro-X Concentrator can concentrate retroviral supernatants created with all commonly used envelopes. A retrovirus expressing DsRed2 was packaged with different retroviral envelopes using the Retro-X Universal Packaging System (Cat. # 631530), and harvested at 72 hr posttransfection. Retro-X Concentrator was used to concentrate dualtropic (10A1), amphotropic (Ampho), ecotropic (Eco), and VSV-G (pantropic) pseudotyped retrovirus samples from 10 ml down to 100 µl (Panels A & B). Crude and concentrated viral stocks were then titrated on HT1080 or NIH 3T3 cells and analyzed via flow cytometry at 72 hr postinfection to determine the percentage of positive cells. Back 631455: Retro-X Concentrator

Overview

Simple mix-and-spin protocol
Hassle-free and easily scaled up for large volumes
No ultracentrifugation required
100X concentration
90% yield
Retro-X Concentrator can also concentrate lentivirus supernatants utilizing the incubation times recommended in the Lenti-X Concentrator Protocol-At-A-Glance

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Applications

Retroviral transduction studies
Increased multiplicity of infection (MOI)
Rescue of low-titer supernatants

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Choose the right viral system

Use these tables to compare the characteristics of lentivirus, retrovirus, adenovirus, and adeno-associated virus (AAV), and select the best recombinant viral gene delivery system for your needs.

Compare viruses Product pages

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