SMARTer universal low input RNA kit—cDNA synthesis for NGS from degraded samples

The SMARTer Universal Low Input RNA Kit for Sequencing provides a simple and efficient solution for generating cDNA from very low amounts of input RNA that is compromised, has a low RIN value, and/or lacks a poly(A) tail. The kit was specifically designed for RNA-Seq applications involving FFPE or laser-captured samples, and works with as little as 200 pg and up to 10 ng of rRNA-depleted material. It outperforms existing methods in terms of gene-body coverage, data reproducibility, read mappability, and ERCC correlation. For upstream rRNA removal from 10-100 ng of input total RNA, we recommend the RiboGone - Mammalian kit.

cDNA produced with this kit is compatible with low-input NGS library preparation kits, including those for Illumina and Ion Torrent.

Cat. #	Product	Size	Price
634940	SMARTer® Universal Low Input RNA Kit for Sequencing	25 Rxns	USD $2318.00
The SMARTer Universal Low Input RNA Kit for Sequencing contains the components needed to synthesize high-quality cDNA from as little as 200 pg of RNA, and includes the Advantage 2 PCR Kit for PCR amplification and validation. The kit utilizes both SMART technology and random priming, and has been designed and validated to prepare cDNA samples for sequencing and quantification with next-generation sequencing platforms. While SMART technology offers unparalleled sensitivity and unbiased amplification of cDNA transcripts, random (universal) priming allows for amplification of damaged RNA and maintains the true representation of the original mRNA transcripts. Both of these factors are critical for transcriptome sequencing and gene expression analysis. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like
634938	SMARTer® Universal Low Input RNA Kit for Sequencing	10 Rxns	USD $1082.00
The SMARTer Universal Low Input RNA Kit for Sequencing contains the components needed to synthesize high-quality cDNA from as little as 200 pg of RNA, and includes the Advantage 2 PCR Kit for PCR amplification and validation. The kit utilizes both SMART technology and random priming, and has been designed and validated to prepare cDNA samples for sequencing and quantification with next-generation sequencing platforms. While SMART technology offers unparalleled sensitivity and unbiased amplification of cDNA transcripts, random (universal) priming allows for amplification of damaged RNA and maintains the true representation of the original mRNA transcripts. Both of these factors are critical for transcriptome sequencing and gene expression analysis. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like

Overview

Sensitivity for analysis of low-input samples—works with 200 pg to 10 ng of rRNA-depleted input (derived from 2–100 ng of total RNA)
Efficient results from compromised samples—an ideal solution for FFPE samples
High quality RNA-seq data—consistent RNA-seq data across the recommended input range (200 pg–10 ng)
- Mappability >80%
- Reproducibility >0.98
- ERCC correlation >0.99
- Number of genes >15,000
Kit includes both RT and PCR enzymes

Interested in more data and FAQs about this product? Visit the NGS Learning Center.

More Information

Applications

Generate cDNA for RNA-seq from very low amounts of RNA (200 pg–10 ng) that:
- Is compromised (e.g., obtained from FFPE or LCM samples)
- Has low RIN values (<8)
- Is not polyadenylated

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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