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Technical notes View data for this product
Home › Products › Next-generation sequencing › RNA-seq › cDNA synthesis for Illumina or Ion Torrent

Next-generation sequencing

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Technical notes View data for this product

SMARTer universal low input RNA kit—cDNA synthesis for NGS from degraded samples

The SMARTer Universal Low Input RNA Kit for Sequencing provides a simple and efficient solution for generating cDNA from very low amounts of input RNA that is compromised, has a low RIN value, and/or lacks a poly(A) tail. The kit was specifically designed for RNA-Seq applications involving FFPE or laser-captured samples, and works with as little as 200 pg and up to 10 ng of rRNA-depleted material. It outperforms existing methods in terms of gene-body coverage, data reproducibility, read mappability, and ERCC correlation. For upstream rRNA removal from 10-100 ng of input total RNA, we recommend the RiboGone - Mammalian kit.

cDNA produced with this kit is compatible with low-input NGS library preparation kits, including those for Illumina and Ion Torrent. 

Cat. # Product Size License Quantity Details
634940 SMARTer® Universal Low Input RNA Kit for Sequencing 25 Rxns USD $2365.00

The SMARTer Universal Low Input RNA Kit for Sequencing contains the components needed to synthesize high-quality cDNA from as little as 200 pg of RNA, and includes the Advantage 2 PCR Kit for PCR amplification and validation. The kit utilizes both SMART technology and random priming, and has been designed and validated to prepare cDNA samples for sequencing and quantification with next-generation sequencing platforms. While SMART technology offers unparalleled sensitivity and unbiased amplification of cDNA transcripts, random (universal) priming allows for amplification of damaged RNA and maintains the true representation of the original mRNA transcripts. Both of these factors are critical for transcriptome sequencing and gene expression analysis.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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634940: SMARTer Universal Low Input RNA Kit for Sequencing

634940: SMARTer Universal Low Input RNA Kit for Sequencing
634938 SMARTer® Universal Low Input RNA Kit for Sequencing 10 Rxns USD $1104.00

The SMARTer Universal Low Input RNA Kit for Sequencing contains the components needed to synthesize high-quality cDNA from as little as 200 pg of RNA, and includes the Advantage 2 PCR Kit for PCR amplification and validation. The kit utilizes both SMART technology and random priming, and has been designed and validated to prepare cDNA samples for sequencing and quantification with next-generation sequencing platforms. While SMART technology offers unparalleled sensitivity and unbiased amplification of cDNA transcripts, random (universal) priming allows for amplification of damaged RNA and maintains the true representation of the original mRNA transcripts. Both of these factors are critical for transcriptome sequencing and gene expression analysis.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

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634938: SMARTer Universal Low Input RNA Kit for Sequencing

634938: SMARTer Universal Low Input RNA Kit for Sequencing

Overview

  • Sensitivity for analysis of low-input samples—works with 200 pg to 10 ng of rRNA-depleted input (derived from 2–100 ng of total RNA)
  • Efficient results from compromised samples—an ideal solution for FFPE samples
  • High quality RNA-seq data—consistent RNA-seq data across the recommended input range (200 pg–10 ng)
    • Mappability >80%
    • Reproducibility >0.98
    • ERCC correlation >0.99
    • Number of genes >15,000
  • Kit includes both RT and PCR enzymes

Interested in more data and FAQs about this product? Visit the NGS Learning Center.

More Information

Applications

  • Generate cDNA for RNA-seq from very low amounts of RNA (200 pg–10 ng) that:
    • Is compromised (e.g., obtained from FFPE or LCM samples)
    • Has low RIN values (<8)
    • Is not polyadenylated

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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  • Human ACE2 stable cell line
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  • RNA extraction and analysis for real-time qPCR
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  • Single-cell cloning of edited hiPS cells
  • mRNA and cDNA synthesis
  • In vitro transcription
  • cDNA synthesis kits
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  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • PCR
  • Most popular polymerases
  • High-yield PCR
  • High-fidelity PCR
  • GC rich PCR
  • PCR master mixes
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  • In-Fusion seamless cloning
  • Competent cells
  • Ligation kits
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