SMARTer® Universal Low Input RNA Kit for Sequencing

Recommended Products

Cat. # Product Size Price License Quantity Details
639207 Advantage® 2 PCR Kit 30 Rxns USD $181.00

This kit is based on Takara's Advantage 2 Polymerase Mix. It contains all reagents needed for a hot-start PCR, including control template and primer mix. This kit is ideal for PCR applications that require high fidelity, such as cDNA amplification or library construction. Enough reagents are supplied for 30 PCR reactions of 50 μl each.

634847 RiboGone™ - Mammalian 24 Rxns USD $1377.00

RiboGone - Mammalian allows for the specific removal of rRNA sequences (5S, 5.8S, 18S, and 28S), as well as mitochondrial rRNA sequences (12S), from human, mouse, or rat total RNA. This kit is designed for use with limited sample amounts (10–100 ng of total RNA), and works with high- or low-quality RNA. Samples processed using the RiboGone kit are ready for cDNA synthesis with random primers.

638325 DNA Standards for Library Quantification 50 Rxns USD $268.00

The DNA Standards for Library Quantification includes four DNA standards (0.01–10 pM) that are used for qPCR-based library quantification prior to Illumina next-generation sequencing. These DNA standards contain specific concentrations of a DNA fragment (538 bp) and are designed specifically for use with the Library Quantification Kit (Cat No. 638324). When used as templates for qPCR with the Library Quantification Kit, the standards will generate 447-bp DNA fragments that can be used to produce a standard curve for determining the concentration of DNA in an Illumina library. The standards are not suitable for use with other qPCR techniques, as they may lead to quantification errors or poor results.

638324 Library Quantification Kit 500 Rxns USD $580.00

The Library Quantification Kit is used for rapid library quantification prior to Illumina next-generation sequencing. The kit contains all of the components needed to determine the amount of library DNA (genomic, cDNA, etc.) in a sample using qPCR. Quantification is accomplished by comparison of the threshold cycle (Ct) of the diluted sample with a standard curve derived from four calibrated DNA standards.

634846 RiboGone™ - Mammalian 6 Rxns USD $394.00

RiboGone - Mammalian allows for the specific removal of rRNA sequences (5S, 5.8S, 18S, and 28S), as well as mitochondrial rRNA sequences (12S), from human, mouse, or rat total RNA. This kit is designed for use with limited sample amounts (10–100 ng of total RNA), and works with high- or low-quality RNA. Samples processed using the RiboGone kit are ready for cDNA synthesis with random primers.

635011 SMARTer-Seq™ Magnetic Separator - PCR Strip Each USD $231.00

The SMARTer-Seq Magnetic Separator - PCR Strip is designed especially for separation of magnetic beads (like AMPure XP beads; Beckman Coulter), from solution. This stand holds up to 24 0.2-ml PCR tubes in two rows, and is designed for capture of magnetic beads in 10–20 minutes. It is ideally suited for magnetic bead-based DNA purification and size selection techniques as used with most of our products for next-generation sequencing.