- Technical notes
- Cellartis Power Primary HEP Medium
- Cellartis DEF-CS 500 Culture System
- Cellartis Enhanced hiPS-HEP cells
- Cellartis hES-MP 002.5
- Cellartis hPS cell-derived cardiomyocytes
- Cellartis iPS Cell to Hepatocyte Differentiation System
- 2i mES/iPSC medium
- 3i mES/iPSC medium
- NDiff 227
- NDiff N2
- Selection guides
RHB-A medium citation list
RHB-A medium is an optimized medium for deriving, maintaining, and expanding neural stem cells. The improved formulation enables efficient neural differentiation from pluripotent stem cells and can also enable differentiation directly into neurons. RHB-A medium can also support central nervous system organotypic slice cultures and culture of glioblastoma and other neural cancer cells. Read below for a citation list of studies in which RHB-A medium was used in peer-reviewed basic, translational, preclinical, and biomedical research.
Abranches, E. et al. Neural Differentiation of Embryonic Stem Cells In Vitro: A Road Map to Neurogenesis in the Embryo. PLoS One 4, e6286 (2009).
Abranches, E., Bekman, E. & Henrique, D. Generation and characterization of a novel mouse embryonic stem cell line with a dynamic reporter of Nanog expression. PLoS One 8, e59928 (2013).
Alvarez-Gonzalez, C. et al. Cord Blood Lin-CD45- Embryonic-Like Stem Cells Are a Heterogeneous Population That Lack Self-Renewal Capacity. PLoS One 8, e67968 (2013).
Aranha, M. M. et al. Apoptosis-associated microRNAs are modulated in mouse, rat and human neural differentiation. BMC Genomics 11, 514 (2010).
Bartesaghi, S. et al. Inhibition of oxidative metabolism leads to p53 genetic inactivation and transformation in neural stem cells. Proc. Natl. Acad. Sci. U. S. A. 112, 1059–64 (2015).
Brooks, M. D. et al. PDE7B is a novel, prognostically significant mediator of glioblastoma growth whose expression is regulated by endothelial cells. PLoS One 9, e107397 (2014).
Castelo-Branco, G. et al. The non-coding snRNA 7SK controls transcriptional termination, poising, and bidirectionality in embryonic stem cells. Genome Biol. 14, R98 (2013).
Chen, Y.-J. J. et al. Use of 'MGE Enhancers' for Labeling and Selection of Embryonic Stem Cell-Derived Medial Ganglionic Eminence (MGE) Progenitors and Neurons. PLoS One 8, e61956 (2013).
Christophorou, M. A. et al. Citrullination regulates pluripotency and histone H1 binding to chromatin. Nature 507, 104–8 (2014).
Conti, L. et al. Niche-independent symmetrical self-renewal of a mammalian tissue stem cell. PLoS Biol. 3, e283 (2005).
Ding, L. et al. A genome-scale RNAi screen for Oct4 modulators defines a role of the Paf1 complex for embryonic stem cell identity. Cell Stem Cell 4, 403–15 (2009).
Diogo, M. M., Henrique, D. & Cabral, J. M. S. Optimization and integration of expansion and neural commitment of mouse embryonic stem cells. Biotechnol. Appl. Biochem. 49, 105 (2008).
Falk, R. et al. Generation of anti-Notch antibodies and their application in blocking Notch signalling in neural stem cells. Methods 58, 69–78 (2012).
Fernandes, A. M. et al. Mouse embryonic stem cell expansion in a microcarrier-based stirred culture system. J. Biotechnol. 132, 227–236 (2007).
Fernandes, T. G. et al. Three-dimensional cell culture microarray for high-throughput studies of stem cell fate. Biotechnol. Bioeng. 106, 106–18 (2010).
Fernandes, T. G., Diogo, M. M., Fernandes-Platzgummer, A., da Silva, C. L. & Cabral, J. M. S. Different stages of pluripotency determine distinct patterns of proliferation, metabolism, and lineage commitment of embryonic stem cells under hypoxia. Stem Cell Res. 5, 76–89 (2010).
Fernandes, T. G., Fernandes-Platzgummer, A. M., da Silva, C. L., Diogo, M. M. & Cabral, J. M. S. Kinetic and metabolic analysis of mouse embryonic stem cell expansion under serum-free conditions. Biotechnol. Lett. 32, 171–9 (2010).
Gangoso, E., Thirant, C., Chneiweiss, H., Medina, J. M. & Tabernero, A. A cell-penetrating peptide based on the interaction between c-Src and connexin43 reverses glioma stem cell phenotype. Cell Death Dis. 5, e1023 (2014).
Gargiulo, G., Serresi, M., Cesaroni, M., Hulsman, D. & van Lohuizen, M. In vivo shRNA screens in solid tumors. Nat. Protoc. 9, 2880–2902 (2014).
Gaspar, N. et al. MGMT-independent temozolomide resistance in pediatric glioblastoma cells associated with a PI3-kinase-mediated HOX/stem cell gene signature. Cancer Res. 70, 9243–52 (2010).
Hansen, D. V, Lui, J. H., Parker, P. R. L. & Kriegstein, A. R. Neurogenic radial glia in the outer subventricular zone of human neocortex. Nature 464, 554–561 (2010).
Hook, L. et al. Non-immortalized human neural stem (NS) cells as a scalable platform for cellular assays. Neurochem. Int. 59, 432–44 (2011).
Hussain, W. et al. Reproducible culture and differentiation of mouse embryonic stem cells using an automated microwell platform. Biochem. Eng. J. 77, 246–257 (2013).
Jaccard, N. et al. Automated method for the rapid and precise estimation of adherent cell culture characteristics from phase contrast microscopy images. Biotechnol. Bioeng. 111, 504–17 (2014).
Khan, Z., Shervington, A., Munje, C. & Shervington, L. The complexity of identifying cancer stem cell biomarkers. Cancer Invest. 31, 404–11 (2013).
Lancini, C. et al. Tight regulation of ubiquitin-mediated DNA damage response by USP3 preserves the functional integrity of hematopoietic stem cells. J. Exp. Med. 211, 1759–77 (2014).
Li, M. et al. Frequent amplification of a chr19q13.41 microRNA polycistron in aggressive primitive neuroectodermal brain tumors. Cancer Cell 16, 533–46 (2009).
Mahfuz Chowdhury, M., Kimura, H., Fujii, T. & Sakai, Y. Induction of alternative fate other than default neuronal fate of embryonic stem cells in a membrane-based two-chambered microbioreactor by cell-secreted BMP4. Biomicrofluidics 6, 14117–14117-13 (2012).
McLaren, D. et al. Automated large-scale culture and medium-throughput chemical screen for modulators of proliferation and viability of human induced pluripotent stem cell-derived neuroepithelial-like stem cells. J. Biomol. Screen. 18, 258–68 (2013).
McMahon, S. S. et al. Engraftment, migration and differentiation of neural stem cells in the rat spinal cord following contusion injury. Cytotherapy 12, 313–25 (2010).
Moeckel, S. et al. Response-predictive gene expression profiling of glioma progenitor cells in vitro. PLoS One 9, e108632 (2014).
Mondragon-Teran, P., Tostoes, R., Mason, C., Lye, G. J. & Veraitch, F. S. Oxygen-controlled automated neural differentiation of mouse embryonic stem cells. Regen. Med. 8, 171–82 (2013).
Onken, J. et al. Versican isoform V1 regulates proliferation and migration in high-grade gliomas. J. Neurooncol. 120, 73–83 (2014).
Payne, N. L. et al. Comparative study on the therapeutic potential of neurally differentiated stem cells in a mouse model of multiple sclerosis. PLoS One 7, e35093 (2012).
Pollard, S. M. et al. Glioma stem cell lines expanded in adherent culture have tumor-specific phenotypes and are suitable for chemical and genetic screens. Cell Stem Cell 4, 568–80 (2009).
Pollard, S. M., Conti, L., Sun, Y., Goffredo, D. & Smith, A. Adherent neural stem (NS) cells from fetal and adult forebrain. Cereb. Cortex 16 Suppl 1, i112–20 (2006).
Pollard, S. M., Wallbank, R., Tomlinson, S., Grotewold, L. & Smith, A. Fibroblast growth factor induces a neural stem cell phenotype in foetal forebrain progenitors and during embryonic stem cell differentiation. Mol. Cell. Neurosci. 38, 393–403 (2008).
Ribeiro, S. et al. Plasmid DNA size does affect nonviral gene delivery efficiency in stem cells. Cell. Reprogram. 14, 130–7 (2012).
Rodrigues, C. A. V, Diogo, M. M., da Silva, C. L. & Cabral, J. M. S. Hypoxia enhances proliferation of mouse embryonic stem cell-derived neural stem cells. Biotechnol. Bioeng. 106, 260–70 (2010).
Ruiz, E. J., Oeztuerk-Winder, F. & Ventura, J.-J. A paracrine network regulates the cross-talk between human lung stem cells and the stroma. Nat. Commun. 5, 3175 (2014).
Sengupta, R. et al. Novel chemical library screen identifies naturally occurring plant products that specifically disrupt glioblastoma-endothelial cell interactions. Oncotarget 6, 18282–92 (2015).
Silva, J. et al. Nanog is the gateway to the pluripotent ground state. Cell 138, 722–37 (2009).
Silva, J. et al. Promotion of reprogramming to ground state pluripotency by signal inhibition. PLoS Biol. 6, e253 (2008).
Stricker, S. H. et al. Widespread resetting of DNA methylation in glioblastoma-initiating cells suppresses malignant cellular behavior in a lineage-dependent manner. Genes Dev. 27, 654–69 (2013).
Strogantsev, R. et al. Allele-specific binding of ZFP57 in the epigenetic regulation of imprinted and non-imprinted monoallelic expression. Genome Biol. 16, 112 (2015).
Sun, Y. et al. CD133 (Prominin) negative human neural stem cells are clonogenic and tripotent. PLoS One 4, e5498 (2009).
Sun, Y. et al. Long-term tripotent differentiation capacity of human neural stem (NS) cells in adherent culture. Mol. Cell. Neurosci. 38, 245–58 (2008).
Sun, Y., Hu, J., Zhou, L., Pollard, S. M. & Smith, A. Interplay between FGF2 and BMP controls the self-renewal, dormancy and differentiation of rat neural stem cells. J. Cell Sci. 124, 1867–77 (2011).
Tarunina, M. et al. Directed differentiation of embryonic stem cells using a bead-based combinatorial screening method. PLoS One 9, e104301 (2014).
Theunissen, T. W. et al. Reprogramming capacity of Nanog is functionally conserved in vertebrates and resides in a unique homeodomain. Development 138, 4853–65 (2011).
Tivnan, A. & McDonald, K. L. Current progress for the use of miRNAs in glioblastoma treatment. Mol. Neurobiol. 48, 757–68 (2013).
Tivnan, A. et al. The tumor suppressor microRNA, miR-124a, is regulated by epigenetic silencing and by the transcriptional factor, REST in glioblastoma. Tumour Biol. 35, 1459–65 (2014).
Torrado, E. F. et al. Directing mouse embryonic neurosphere differentiation toward an enriched neuronal population. Int. J. Dev. Neurosci. 37, 94–9 (2014).
Vinci, M., Box, C. & Eccles, S. A. Three-dimensional (3D) tumor spheroid invasion assay. J. Vis. Exp. e52686 (2015). doi:10.3791/52686
Yasuhara, N. et al. Importin alpha subtypes determine differential transcription factor localization in embryonic stem cells maintenance. Dev. Cell 26, 123–35 (2013).
Ying, Q.-L., Stavridis, M., Griffiths, D., Li, M. & Smith, A. Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture. Nat. Biotechnol. 21, 183–6 (2003).
Zhu, T. et al. An acellular cerebellar biological scaffold: Preparation, characterization, biocompatibility and effects on neural stem cells. Brain Res. Bull. 113, 48–57 (2015).
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2020 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.