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iMatrix-511 citation list
iMatrix-511 is a recombinant laminin-511 E8 fragment that can be used as a pluripotent stem cell culture substrate to retain pluripotency and a stable karyotype. The fragments enable defined, feeder- and xeno-free culture conditions and provide greater adhesive properties than either full-length laminin, vitronectin, or undefined basement membrane reagents such as Matrigel. Read below for a citation list of studies in which iMatrix-511 was used in peer-reviewed basic, translational, preclinical, and biomedical research.
Burridge, P. W. et al. Chemically defined generation of human cardiomyocytes. Nat. Methods 11, 855–60 (2014).
Kawase, E. Efficient Expansion of Dissociated Human Pluripotent Stem Cells Using a Synthetic Substrate. Methods Mol. Biol. 1307, 61–9 (2016).
Kim, S.-I. et al. Inducible Transgene Expression in Human iPS Cells Using Versatile All-in-One piggyBac Transposons. Methods Mol. Biol. 1357, 111–31 (2016).
Miyazaki, T. & Kawase, E. Efficient and scalable culture of single dissociated human pluripotent stem cells using recombinant E8 fragments of human laminin isoforms. Curr. Protoc. Stem Cell Biol. 32, 1C.18.1–8 (2015).
Miyazaki, T. & Suemori, H. Cryopreservation of human pluripotent stem cells: a general protocol. Methods Mol. Biol. 1235, 97–104 (2015).
Nakagawa, M. et al. A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells. Sci. Rep. 4, 3594 (2014).
Okeyo, K. O. et al. Cell Adhesion Minimization by a Novel Mesh Culture Method Mechanically Directs Trophoblast Differentiation and Self-Assembly Organization of Human Pluripotent Stem Cells. Tissue Eng. Part C. Methods 21, 1105–15 (2015).
Okumura, N. et al. Laminin-511 and -521 Enable Efficient In Vitro Expansion of Human Corneal Endothelial Cells. Investig. Opthalmology Vis. Sci. 56, 2933 (2015).
Sasaki, K. et al. Robust In Vitro Induction of Human Germ Cell Fate from Pluripotent Stem Cells. Cell Stem Cell 17, 178–94 (2015).
Takashima, Y. et al. Resetting transcription factor control circuitry toward ground-state pluripotency in human. Cell 158, 1254–69 (2014).
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