- Technical notes
- Cellartis Power Primary HEP Medium
- Cellartis DEF-CS 500 Culture System
- Cellartis Enhanced hiPS-HEP cells
- Cellartis hES-MP 002.5
- Cellartis hPS cell-derived cardiomyocytes
- Cellartis iPS Cell to Hepatocyte Differentiation System
- 2i mES/iPSC medium
- 3i mES/iPSC medium
- NDiff 227
- NDiff N2
- Selection guides
Cellartis 3i mES/iPSC culture medium citation list
Cellartis 3i mES/iPSC Culture Medium (formerly iSTEM medium) is a defined cell culture medium for deriving, maintaining, and propagating mouse pluripotent stem cells. The medium utilizes a three small molecule inhibitor-based method (3i), which blocks FGFR (SU5402), ERK (PD184352), and GSK3 (CHIR99021). This technique blocks differentiating-inducing signals, promotes cell survival, and maintains pluripotency without the need for additional stimulatory cytokines. This method is a powerful way to keep pluripotent stem cells in a basal, ground-state that enables germline-competency. Read below for a citation list of studies in which 3i mES/iPSC (iSTEM) medium was used in peer-reviewed basic, translational, preclinical, and biomedical research.
Barbosa, H. S. C., Fernandes, T. G., Dias, T. P., Diogo, M. M. & Cabral, J. M. S. New insights into the mechanisms of embryonic stem cell self-renewal under hypoxia: a multifactorial analysis approach. PLoS One 7, e38963 (2012).
Fernandes, T. G., Diogo, M. M., Fernandes-Platzgummer, A., da Silva, C. L. & Cabral, J. M. S. Different stages of pluripotency determine distinct patterns of proliferation, metabolism, and lineage commitment of embryonic stem cells under hypoxia. Stem Cell Res. 5, 76–89 (2010).
Filipczyk, A. et al. Biallelic expression of nanog protein in mouse embryonic stem cells. Cell Stem Cell 13, 12–3 (2013).
Hirabayashi, M. et al. A retrospective analysis of germline competence in rat embryonic stem cell lines. Transgenic Res. 22, 411–6 (2013).
Hirabayashi, M. et al. Effect of leukemia inhibitory factor and forskolin on establishment of rat embryonic stem cell lines. J. Reprod. Dev. 60, 78–82 (2014).
Iijima, S. et al. Effect of different culture conditions on establishment of embryonic stem cells from BALB/cAJ and NZB/BINJ mice. Cell. Reprogram. 12, 679–88 (2010).
Kiyonari, H., Kaneko, M., Abe, S. & Aizawa, S. Three inhibitors of FGF receptor, ERK, and GSK3 establishes germline-competent embryonic stem cells of C57BL/6N mouse strain with high efficiency and stability. Genesis 48, 317–27 (2010).
Ohta, H. et al. Male germline and embryonic stem cell lines from NOD mice: efficient derivation of GS cells from a nonpermissive strain for ES cell derivation. Biol. Reprod. 81, 1147–53 (2009).
Pashaiasl, M., Khodadadi, K., Richings, N. M., Holland, M. K. & Verma, P. J. Cryopreservation and long-term maintenance of bovine embryo-derived cell lines. Reprod. Fertil. Dev. 25, 707–18 (2013).
Rosselló, R. A. et al. Mammalian genes induce partially reprogrammed pluripotent stem cells in non-mammalian vertebrate and invertebrate species. Elife 2, e00036 (2013).
Ying, Q.-L. et al. The ground state of embryonic stem cell self-renewal. Nature 453, 519–23 (2008).
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