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  • ‹ Back to Epigenetics and small RNA sequencing
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SMARTer smRNA-Seq Kit for Illumina—sequence small RNAs with high sensitivity and minimal bias

Discovery and analysis of small non-coding RNAs (smRNAs) has become an important part of understanding gene expression regulation. Some of the well-known small RNA species are miRNAs, siRNAs, piRNAs, and snoRNAs, many of which range in size from 20 to 30 nt. The SMARTer smRNA-Seq Kit for Illumina has been specifically designed to generate high-quality Illumina-ready sequencing libraries from low-input amounts of small RNAs such as these.

This kit was developed to work directly from 1 ng–2 µg inputs of total RNA or enriched small RNA samples, and incorporates features from the industry-leading SMART-Seq v4 kit, including SMART technology (Switching Mechanism at 5’ End of RNA Template) and locked nucleic acids (LNAs). Libraries are generated in a ligation-free manner, ensuring that diverse small RNA species are represented with minimal bias.

Cat. # Product Size Price License Quantity Details
635031 SMARTer® smRNA-Seq Kit for Illumina® 96 Rxns $4416.00

The SMARTer smRNA-Seq Kit for Illumina is used to generate small RNA-seq libraries for sequencing on Illumina platforms. This kit was developed to work directly with total RNA or enriched small RNA (including microRNA), for inputs ranging from 1 ng–2 μg. By incorporating features of the SMARTer Stranded RNA-Seq kits, including our proprietary SMART (Switching Mechanism at the 5' end of RNA Template) technology, and primers that include locked nucleic acids (LNAs), this kit allows users to analyze a wide range of smRNA species and generate sequencing libraries of considerable complexity from as little as 1 ng of input material. Illumina adapter and index sequences are incorporated in a ligation-free manner during library amplification, ensuring that diverse smRNA species are represented with minimal bias.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data Resources

Back

Demonstrating the accuracy of the SMARTer approach for small RNA-seq

Demonstrating the accuracy of the SMARTer approach for small RNA-seq
Demonstrating the accuracy of the SMARTer approach for small RNA-seq. Sequencing libraries were generated from an equimolar pool of 963 synthetic miRNAs (miRXplore Universal Reference) using the SMARTer smRNA-Seq Kit for Illumina (1 ng input; purple), or a small RNA-seq kit from a different vendor (Competitor N) employing an adapter ligation method (100 ng input; blue). Following sequencing, mapping, and counting of reads, miRNA expression levels (Y axis, log scale) were normalized, resulting in an expected expression level equal to 1 for each miRNA, and a 2-fold cutoff was assigned both above and below the expected expression level (indicated by two horizontal lines). For visualization purposes, miRNAs are ranked along the X axis in order of expression level.

Back

Reproducibility of SMARTer small RNA-seq data

Reproducibility of SMARTer small RNA-seq data
Reproducibility of SMARTer small RNA-seq data. Sequencing libraries were generated in parallel from the indicated input amounts of human brain total RNA using the SMARTer smRNA-Seq Kit for Illumina, and size selected using the BluePippin system. Following sequencing, data processing, and mapping, expression levels of miRNAs identified for each library were quantified and plotted on correlation diagrams. Panel A. Correlation of miRNA expression levels for experimental replicates involving 1 ng inputs. Panel B. Correlation of miRNA expression levels for 2 µg vs. 1 ng inputs.

Back

Schematic of technology used by the SMARTer smRNA-Seq Kit for Illumina

Schematic of technology used by the SMARTer smRNA-Seq Kit for Illumina
Schematic of technology used by the SMARTer smRNA-Seq Kit for Illumina. SMART technology is used in a ligation-free workflow to generate sequencing libraries for Illumina platforms. Input RNA is first polyadenylated in order to provide a priming sequence for an oligo(dT) primer. cDNA synthesis is primed by the 3’ smRNA dT Primer, which incorporates an adapter sequence (green) at the 5’ end of each first-strand cDNA molecule. When the MMLV-derived PrimeScript Reverse Transcriptase (RT) reaches the 5’ end of each RNA template, it adds non-templated nucleotides which are bound by the SMART smRNA Oligo—enhanced with locked nucleic acid (LNA) technology for greater sensitivity. In the template-switching step, PrimeScript RT uses the SMART smRNA Oligo as a template for the addition of a second adapter sequence (purple) to the 3’end of each first-strand cDNA molecule. In the next step, full-length Illumina adapters (including indexes for sample multiplexing) are added during PCR amplification. The Forward PCR Primer binds to the sequence added by the SMART smRNA Oligo, while the Reverse PCR Primer binds to the sequence added by the 3’ smRNA dT Primer. Resulting library cDNA molecules include adapters required for clustering on an Illumina flow cell (P5 shown in light blue, P7 shown in red), Illumina TruSeq® HT indexes (Index 2 [i5] shown in orange, Index 1 [i7] shown in yellow), and regions bound by the Read Primer 1 or Read Primer 2 sequencing primers (shown in purple and green, respectively). Note that adapters included in the final library add 153 bp to the size of RNA-derived insert sequences.
635030 SMARTer® smRNA-Seq Kit for Illumina® 48 Rxns $2448.00

The SMARTer smRNA-Seq Kit for Illumina is used to generate small RNA-seq libraries for sequencing on Illumina platforms. This kit was developed to work directly with total RNA or enriched small RNA (including microRNA), for inputs ranging from 1 ng–2 μg. By incorporating features of the SMARTer Stranded RNA-Seq kits, including our proprietary SMART (Switching Mechanism at the 5' end of RNA Template) technology, and primers that include locked nucleic acids (LNAs), this kit allows users to analyze a wide range of smRNA species and generate sequencing libraries of considerable complexity from as little as 1 ng of input material. Illumina adapter and index sequences are incorporated in a ligation-free manner during library amplification, ensuring that diverse smRNA species are represented with minimal bias.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data Resources

Back

Demonstrating the accuracy of the SMARTer approach for small RNA-seq

Demonstrating the accuracy of the SMARTer approach for small RNA-seq
Demonstrating the accuracy of the SMARTer approach for small RNA-seq. Sequencing libraries were generated from an equimolar pool of 963 synthetic miRNAs (miRXplore Universal Reference) using the SMARTer smRNA-Seq Kit for Illumina (1 ng input; purple), or a small RNA-seq kit from a different vendor (Competitor N) employing an adapter ligation method (100 ng input; blue). Following sequencing, mapping, and counting of reads, miRNA expression levels (Y axis, log scale) were normalized, resulting in an expected expression level equal to 1 for each miRNA, and a 2-fold cutoff was assigned both above and below the expected expression level (indicated by two horizontal lines). For visualization purposes, miRNAs are ranked along the X axis in order of expression level.

Back

Reproducibility of SMARTer small RNA-seq data

Reproducibility of SMARTer small RNA-seq data
Reproducibility of SMARTer small RNA-seq data. Sequencing libraries were generated in parallel from the indicated input amounts of human brain total RNA using the SMARTer smRNA-Seq Kit for Illumina, and size selected using the BluePippin system. Following sequencing, data processing, and mapping, expression levels of miRNAs identified for each library were quantified and plotted on correlation diagrams. Panel A. Correlation of miRNA expression levels for experimental replicates involving 1 ng inputs. Panel B. Correlation of miRNA expression levels for 2 µg vs. 1 ng inputs.

Back

Schematic of technology used by the SMARTer smRNA-Seq Kit for Illumina

Schematic of technology used by the SMARTer smRNA-Seq Kit for Illumina
Schematic of technology used by the SMARTer smRNA-Seq Kit for Illumina. SMART technology is used in a ligation-free workflow to generate sequencing libraries for Illumina platforms. Input RNA is first polyadenylated in order to provide a priming sequence for an oligo(dT) primer. cDNA synthesis is primed by the 3’ smRNA dT Primer, which incorporates an adapter sequence (green) at the 5’ end of each first-strand cDNA molecule. When the MMLV-derived PrimeScript Reverse Transcriptase (RT) reaches the 5’ end of each RNA template, it adds non-templated nucleotides which are bound by the SMART smRNA Oligo—enhanced with locked nucleic acid (LNA) technology for greater sensitivity. In the template-switching step, PrimeScript RT uses the SMART smRNA Oligo as a template for the addition of a second adapter sequence (purple) to the 3’end of each first-strand cDNA molecule. In the next step, full-length Illumina adapters (including indexes for sample multiplexing) are added during PCR amplification. The Forward PCR Primer binds to the sequence added by the SMART smRNA Oligo, while the Reverse PCR Primer binds to the sequence added by the 3’ smRNA dT Primer. Resulting library cDNA molecules include adapters required for clustering on an Illumina flow cell (P5 shown in light blue, P7 shown in red), Illumina TruSeq® HT indexes (Index 2 [i5] shown in orange, Index 1 [i7] shown in yellow), and regions bound by the Read Primer 1 or Read Primer 2 sequencing primers (shown in purple and green, respectively). Note that adapters included in the final library add 153 bp to the size of RNA-derived insert sequences.
635029 SMARTer® smRNA-Seq Kit for Illumina® 12 Rxns $720.00

The SMARTer smRNA-Seq Kit for Illumina is used to generate small RNA-seq libraries for sequencing on Illumina platforms. This kit was developed to work directly with total RNA or enriched small RNA (including microRNA), for inputs ranging from 1 ng–2 μg. By incorporating features of the SMARTer Stranded RNA-Seq kits, including our proprietary SMART (Switching Mechanism at the 5' end of RNA Template) technology, and primers that include locked nucleic acids (LNAs), this kit allows users to analyze a wide range of smRNA species and generate sequencing libraries of considerable complexity from as little as 1 ng of input material. Illumina adapter and index sequences are incorporated in a ligation-free manner during library amplification, ensuring that diverse smRNA species are represented with minimal bias.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data Resources

Back

Demonstrating the accuracy of the SMARTer approach for small RNA-seq

Demonstrating the accuracy of the SMARTer approach for small RNA-seq
Demonstrating the accuracy of the SMARTer approach for small RNA-seq. Sequencing libraries were generated from an equimolar pool of 963 synthetic miRNAs (miRXplore Universal Reference) using the SMARTer smRNA-Seq Kit for Illumina (1 ng input; purple), or a small RNA-seq kit from a different vendor (Competitor N) employing an adapter ligation method (100 ng input; blue). Following sequencing, mapping, and counting of reads, miRNA expression levels (Y axis, log scale) were normalized, resulting in an expected expression level equal to 1 for each miRNA, and a 2-fold cutoff was assigned both above and below the expected expression level (indicated by two horizontal lines). For visualization purposes, miRNAs are ranked along the X axis in order of expression level.

Back

Reproducibility of SMARTer small RNA-seq data

Reproducibility of SMARTer small RNA-seq data
Reproducibility of SMARTer small RNA-seq data. Sequencing libraries were generated in parallel from the indicated input amounts of human brain total RNA using the SMARTer smRNA-Seq Kit for Illumina, and size selected using the BluePippin system. Following sequencing, data processing, and mapping, expression levels of miRNAs identified for each library were quantified and plotted on correlation diagrams. Panel A. Correlation of miRNA expression levels for experimental replicates involving 1 ng inputs. Panel B. Correlation of miRNA expression levels for 2 µg vs. 1 ng inputs.

Back

Schematic of technology used by the SMARTer smRNA-Seq Kit for Illumina

Schematic of technology used by the SMARTer smRNA-Seq Kit for Illumina
Schematic of technology used by the SMARTer smRNA-Seq Kit for Illumina. SMART technology is used in a ligation-free workflow to generate sequencing libraries for Illumina platforms. Input RNA is first polyadenylated in order to provide a priming sequence for an oligo(dT) primer. cDNA synthesis is primed by the 3’ smRNA dT Primer, which incorporates an adapter sequence (green) at the 5’ end of each first-strand cDNA molecule. When the MMLV-derived PrimeScript Reverse Transcriptase (RT) reaches the 5’ end of each RNA template, it adds non-templated nucleotides which are bound by the SMART smRNA Oligo—enhanced with locked nucleic acid (LNA) technology for greater sensitivity. In the template-switching step, PrimeScript RT uses the SMART smRNA Oligo as a template for the addition of a second adapter sequence (purple) to the 3’end of each first-strand cDNA molecule. In the next step, full-length Illumina adapters (including indexes for sample multiplexing) are added during PCR amplification. The Forward PCR Primer binds to the sequence added by the SMART smRNA Oligo, while the Reverse PCR Primer binds to the sequence added by the 3’ smRNA dT Primer. Resulting library cDNA molecules include adapters required for clustering on an Illumina flow cell (P5 shown in light blue, P7 shown in red), Illumina TruSeq® HT indexes (Index 2 [i5] shown in orange, Index 1 [i7] shown in yellow), and regions bound by the Read Primer 1 or Read Primer 2 sequencing primers (shown in purple and green, respectively). Note that adapters included in the final library add 153 bp to the size of RNA-derived insert sequences.

Overview

  • Analysis of diverse small RNA species—Sequencing library prep of small RNAs ranging in size from 15–150 nt
  • Flexible sample inputs—Start from 1 ng–2 µg of total RNA or enriched small RNA
  • Illumina-ready sequencing libraries—Ligation-free incorporation of Illumina adapters and indexes for sample multiplexing
  • Quick, single-tube workflow—Generate sequencing libraries in ~3 hours (not including validation and post-PCR size-selection steps)

More Information

Applications

  • Illumina-ready sequencing library preparation, specifically for small RNAs ranging in size from 15–150 nt

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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Capturem Trypsin for a rapid, efficient mass spectometry workflow at room temperature.

Speed up your mass spec workflow

Capturem Trypsin provides rapid, efficient, and complete digestion of protein samples, allowing an uninterrupted mass spectometry workflow at room temperature for downstream protein analysis. This product utilizes our novel Capturem technology in a spin column format with membrane-immobilized trypsin. Capturem Trypsin Columns may be used to completely digest protein samples in less than a minute with digestion efficiencies (protein coverage) comparable to or better than those obtained using in-solution trypsin digestion.

Capturem trypsin technology

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