Retroviral packaging cell lines and systems
The Retro-X Universal Packaging System is our premium retroviral packaging system featuring the GP2-293 cell line. All four commonly used envelopes are supplied on separate vectors including VSV-G, eco, ampho, and 10A1 to allow you to choose the tropism that is most appropriate for your target cells. The envelope vector is cotransfected with your retroviral expression vector, and high titers of amphotropic, ecotropic, dualtropic, or pantropic virions can be obtained in less than 48 hours.
The Retro-X Universal Packaging System is our premium retroviral packaging system featuring the GP2-293 cell line. All four commonly used envelopes are supplied on separate vectors including VSV-G, eco, ampho, and 10A1 to allow you to choose the tropism that is most appropriate for your target cells. The envelope vector is cotransfected with your retroviral expression vector, and high titers of amphotropic, ecotropic, dualtropic, or pantropic virions can be obtained in less than 48 hours.
RetroPack PT67 Cell Line
RetroPack PT67 is the recommended packaging cell line for creating a stable producer cell line for continuous production of retrovirus. If you wish to make retrovirus via transient transfection, we recommend the Retro-X Universal Packaging System. This retroviral packaging system includes an NIH/3T3-based packaging cell line that expresses the 10A1 viral envelope. Virus packaged from PT67 cells can be used to infect a broad range of mammalian cells including human mouse and rat cell types. The virus is able to enter cells via two different surface molecules, the amphotropic retrovirus receptor (Ram-1) and the GALV receptor.
Pantropic Retroviral Expression System
The Pantropic Retroviral Expression System also features the GP2-293 packaging cell line that expresses gamma-retroviral gag and pol proteins together with pVSV-G. Upon transient transfection of pVSV-G the cell line expresses an envelope glycoprotein from the vesicular stomatitis virus that is not dependent on a cell surface receptor; instead, it mediates viral entry through lipid binding and plasma membrane fusion. Two transfer expression vectors are included with the pantropic system, giving you the choice of using a retroviral 5' LTR or the Drosophila hsp70 promoter to express your gene of interest.
Overview
- We recommend the Retro-X Universal Packaging System for most applications
- This system produces the highest titers
- GP2-293 cells are easy to transfect which leads to very high titers
- It is supplied with all four commonly used envelopes for great flexibility (including VSV-G and Eco)
- Retrovirus is produced by transient transfection of the envelope vector and retrovirus vector
- Use RetroPack cells if you wish to create a stable producer cell line
- All retroviral packaging cell lines and systems are compatible with all retroviral vectors sold by Takara Bio, including all MMLV- and MSCV-based retroviruses.
More Information
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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Premium packaging system for recombinant retrovirus production
Our retroviral packaging system includes the GP2-293 Packaging Cell Line, which expresses viral Gag and Pol proteins, and 4 envelope expression vectors. Cotransfecting an envelope vector with your retroviral construct allows you to select the ideal tropism for optimal transduction of your target cells.
Read the tech note Retrovirus learning centerTakara Bio USA, Inc.
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