- Capturem rapid purification technology
- His-tag purification
- Antibody purification
- Phosphoprotein and glycoprotein purification
- Other tag purification
- Mass spectrometry digestion reagents
- Matchmaker Gold yeast two-hybrid systems
- Expression systems
FAQs about Capturem technology
There is a constant need for faster, more efficient antibody and protein purification processes at any scale. Capturem membrane technology allows for purification directly from complex matrices, such as cell supernatants, in minutes. This technology also provides highly purified and concentrated antibodies and his-tagged proteins, even from samples containing additives not compatible with other purification technologies.
Find out more about Capturem technology: click on the expandable tabs below to view the answers to the questions that we are most frequently asked.
General questions about Capturem technology
Can you reuse Capturem plates and columns?
Capturem devices are designed for single use and have not been tested for multiple uses.
How many elutions are required to recover most of your bound protein or antibody?
That depends on the specific chemistries involved. We find that his-tagged and Protein A-based purification typically require 1–2 elutions, while Protein G and some streptavidin chemistries (e.g., Ab-Ab capture) require 2–3 elutions.
What is the yield or capacity of Capturem plates and columns?
Is it a problem if the column or plate membranes dry out between steps when centrifuging or using vacuum filtration?
We have not seen any problems resulting from membranes drying out between steps, whether we are using centrifugation, vacuum filtration, or positive pressure manifolds. We expect the liquid to be fully removed after a 3-min centrifugation step.
What are the recommended storage conditions for Capturem plates and columns?
All Capturem products can be stored at room temperature.
What sort of clarification procedure is required before loading samples onto Capturem plates and columns?
We recommend pre-clarification by centrifugation. If any sort of clogging occurs, we also recommend filtering with a filter smaller than 1.2 microns (for example, a 0.8-micron filtration unit). Other methods for dealing with viscous samples, such as the use of DNAse I or lysozyme, are discussed in our xTractor Buffer and xTractor Buffer Kit User Manual.
What pH range can Capturem membranes tolerate?
Capturem membranes are highly stable nylon-based membranes that remain stable from pH 1 to pH 13. The pH range we recommend for each Capturem product is based on its particular chemistry, and is specified in its respective Protocol-At-A-Glance. For example, we recommend a pH of 8 for Protein A purification and a pH of 5 for Protein G purification.
Capturem technology technical notes
Capturem his-tagged purification protocols
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2018 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.