High-throughput isolation of genomic DNA from tissue—NucleoSpin 8/96 Tissue
NucleoSpin 8/96 Tissue is designed for the rapid and simultaneous preparation of highly pure genomic DNA from animal and human tissue (e.g., mouse and rat tails) for varying throughputs in a flexible 8-well strip format and for high-throughput applications in a 96-well format. Up to 30–40 µg of pure genomic DNA (typical yields are 5–15 µg) can be prepared from a 1.2-cm mouse tail tip section (age of mice is 4–6 weeks). Output DNA can be used directly for PCR, Southern blotting, or any kind of enzymatic reaction.
NucleoSpin 8/96 Tissue is designed for the rapid and simultaneous preparation of highly pure genomic DNA from animal and human tissue (e.g., mouse and rat tails) for varying throughputs in a flexible 8-well strip format and for high-throughput applications in a 96-well format. Up to 30–40 µg of pure genomic DNA (typical yields are 5–15 µg) can be prepared from a 1.2-cm mouse tail tip section (age of mice is 4–6 weeks). Output DNA can be used directly for PCR, Southern blotting, or any kind of enzymatic reaction.
NucleoSpin Tissue Binding Strips/Plates can be used under vacuum or by centrifugation. With the NucleoSpin 8/96 Tissue method, lysis is achieved by incubation of the samples in a solution containing detergents and Proteinase K at 56°C. Appropriate conditions for binding of DNA to the silica membranes of the NucleoSpin Tissue Binding Strip/Plates are created by addition of large amounts of chaotropic ions and ethanol to the lysate. The binding process is reversible and specific to nucleic acids. Contaminants are removed by washing with two different wash buffers. Pure genomic DNA is finally eluted under low ionic strength in a slightly alkaline elution buffer.
The NucleoSpin 96 Tissue Core kit provides the buffers, Proteinase K, and NucleoSpin Tissue Binding Plates only. Accessory plates (e.g., lysis plates, elution plates) are not provided with the core kit. The user can select additional consumables from a variety of suitable accessory plates according to individual needs, for the highest flexibility.
Overview
- Processing possible under vacuum or by centrifugation
- Suitable for manual and automated processing
- Innovative MN Wash Plate minimizes risk of cross-contamination
More Information
| NucleoSpin 8 Tissue | NucleoSpin 96 Tissue NucleoSpin 96 Tissue Core Kit* |
|
| Technology | Silica membrane | Silica membrane |
| Format | 8-well strips | 96-well plates |
| Processing | Manual or automated, vacuum or centrifugation |
Manual or automated, vacuum or centrifugation |
| Starting material | <20 mg tissue, <106 cultured cells, bacteria |
<20 mg tissue, <106 cultured cells, bacteria |
| Fragment size | 300 bp–>30 kb | 300 bp–>30 kb |
| Typical yield | 15–25 µg | 15–25 µg |
| A260/280 | 1.8–1.9 | 1.8–1.9 |
| Elution volume | 100–200 µl | 100–200 µl |
| Preparation time | 20 min/6 strips (excl. lysis) | 60 min/plate (excl. lysis) |
| Binding capacity | 40 µg | 40 µg |
* NucleoSpin 96 Tissue Core Kit: Reduced kit composition together with a large variety of separately available accessories allow optimal adjustment of the kits to individual needs.
Applications
Output DNA is ready to use for:
- PCR
- Southern blotting
- Enzymatic reactions
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Designed for extra small samples
Purify DNA from small samples such as microdissected tissue, small blood samples, or small amounts of cells. Based on reliable silica-membrane technology, NucleoSpin Tissue XS overcomes the limitations of conventional kits using an innovative new column design with a funnel-shaped ring.
Learn more All genomic DNA kitsTakara Bio USA, Inc.
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