Lentivirus purification kits

Purification of lentivirus from packaging supernatants

The Lenti-X Maxi Purification Kit produces outstanding yields of highly purified virus from crude supernatants. The gravity column-based protocol is fast, simple, and effective, and superior to filter-based purification systems, which can damage fragile lentiviral particles and reduce yields. The gravity-flow column gently retains virus particles from the supernatant while unbound material flows through the column during the wash steps.

Why purify your lentivirus?

Lentivirus purification enables you to remove cellular contaminants that could otherwise adversely affect your transduction experiments. Crude supernatants contain residual plasmid DNA, uncharacterized infection and transduction inhibitors, cellular and serum proteins, and highly immunogenic viral proteins, nucleic acids, and virus fragments. Before using lentivirus with sensitive target cells or for in vivo applications, it is essential to remove these contaminants by purifying the lentivirus.

Simple procedure

Our lentivirus purification columns arrive prepacked and ready to use. Simply add the 10X binding buffer to your supernatant sample (9–45 ml) and apply it to the column. The sample and buffers flow through the resin by gravity—just like a column-based plasmid prep. Impurities are removed during two column washes, and purified lentivirus is recovered in 3 ml of elution buffer.

Prevent pseudotransduction

Using purified lentivirus stocks also prevents pseudotransduction, in which high levels of recombinant protein in the crude supernatant are passively transferred to target cells during infection. Pseudotransduction of target cells can mask the de novo expression that is expected from your transduced lentivirus.

Higher yield and purity

A gentle purification procedure is the key to maintaining viral infectivity and producing excellent lentivirus yields of 60–80%. Anion exchange-based membrane systems offer much lower recoveries, usually less than 20%. Lenti-X columns also attain a much higher degree of virus purity than filter-based systems. In fact, purified Lenti-X samples contain very little detectable protein, while filter-purified virus preparations contain high levels of extraneous proteins that copurify with the virus.

More Less

Cat. #	Product	Size	Price
631234	Lenti-X™ Maxi Purification Kit	5 Preps	USD $621.00
The Lenti-X Maxi Purification Kit purifies lentivirus from crude supernatants, and produces outstanding yields of highly purified virus. The gravity column-based protocol is fast, simple, and effective. The Lenti-X column gently retains charged virus particles from the supernatant, while unbound material exits the column during the wash steps. The purified virus that is eluted from the column is recovered fully intact and fully functional. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back Lentivirus purification Lentivirus purification.The Lenti-X gravity column-based method (bind, wash, elute) is extremely simple, effective, and preserves virus much better than syringe filter-based methods. Back Clontech's lentiviral purifcation kit generates high yield and high purity Clontech's lentiviral purification kit generates high yield and high purity. Lentivirus was generated using our high-titer lentiviral packaging system and purified. Panel A. To determine yield the lentiviral titer was measured before and after purification using either titration by qRT-PCR (RNA copies) or flow cytometry/fluorescence (IFU). The mean values from seven experiments are shown. Panel B. Equivalent amounts of purified lentivirus (1 x 10⁵ IFU) prepared using either Lenti-X or a filter-based system (Vendor M & Vendor S) were subjected to SDS-PAGE and silver-stained. The Lenti-X sample clearly contained far fewer contaminating proteins at equivalent IFU than either sample prepared from the filter-based systems. Back Collection Rack L Collection Rack L. Once assembled, the rack holds three columns (e.g., lentiviral purification columns) and three collection tubes (50 ml conical tubes) safely and securely. Back 631234: Lenti-X Maxi Purification Kit
631233	Lenti-X™ Maxi Purification Kit	2 Preps	USD $328.00
The Lenti-X Maxi Purification Kit purifies lentivirus from crude supernatants, and produces outstanding yields of highly purified virus. The gravity column-based protocol is fast, simple, and effective. The Lenti-X column gently retains charged virus particles from the supernatant, while unbound material exits the column during the wash steps. The purified virus that is eluted from the column is recovered fully intact and fully functional. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Resources Back Lentivirus purification Lentivirus purification.The Lenti-X gravity column-based method (bind, wash, elute) is extremely simple, effective, and preserves virus much better than syringe filter-based methods. Back Clontech's lentiviral purifcation kit generates high yield and high purity Clontech's lentiviral purification kit generates high yield and high purity. Lentivirus was generated using our high-titer lentiviral packaging system and purified. Panel A. To determine yield the lentiviral titer was measured before and after purification using either titration by qRT-PCR (RNA copies) or flow cytometry/fluorescence (IFU). The mean values from seven experiments are shown. Panel B. Equivalent amounts of purified lentivirus (1 x 10⁵ IFU) prepared using either Lenti-X or a filter-based system (Vendor M & Vendor S) were subjected to SDS-PAGE and silver-stained. The Lenti-X sample clearly contained far fewer contaminating proteins at equivalent IFU than either sample prepared from the filter-based systems. Back Collection Rack L Collection Rack L. Once assembled, the rack holds three columns (e.g., lentiviral purification columns) and three collection tubes (50 ml conical tubes) safely and securely. Back 631233: Lenti-X Maxi Purification Kit

Overview

Higher purity and yield than other purification technologies
Concentrates virus up to 10-fold
Simple and gentle gravity-flow protocol
Specially designed collection rack can be purchased with the kit (Cat. # 631245) or separately (Cat. # 631246)

More efficient and consistent lentiviral transductions, and healthier target cells
Efficiently removes transduction inhibitors and cytotoxic contaminants

More Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

learn about our uniquely simple to use lentiviral packaging system

Tips for a successful transduction

What are the most critical aspects that affect a lentiviral transduction experiment? Learn about upstream and downstream factors affecting lentiviral titer and transduction efficiency.

Tips Lentivirus learning center

Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2025 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

Lentivirus purification kits

Why purify your lentivirus?

Simple procedure

Prevent pseudotransduction

Higher yield and purity

Notice to purchaser