Concentrate lentiviral supernatants without ultracentrifugation

Effortlessly concentrate lentiviral supernatants 100-fold

Lenti-X Concentrator provides a fast, simple, and highly efficient method for concentrating any lentiviral stock. In the simple protocol, you just mix your lentiviral supernatant with the lentivirus concentration reagent, incubate for a short period, and spin the mixture in a standard centrifuge.

100-fold increase in titer

You will increase your lentiviral titer by up to 100-fold in ~1 hr, and obtain excellent recoveries with no ultracentrifugation. Lenti-X Concentrator works for all lentiviral supernatants, including those made from any Takara Bio-brand Lenti-X system, and the procedure can be scaled up or down to best suit your needs.

Far simpler than ultracentrifugation

Compared to ultracentrifugation, the Lenti-X Concentrator is more flexible in terms of scale, is easier to use, and requires no specialized equipment.

Scalable for supernatants of any volume, any titer

The Lenti-X Concentrator reagent is a 4X concentrate so that can be added to any volume of supernatant containing any amount of virus or any starting titer. In our hands, scalability has been successful for supernatant volumes up to 250 ml.

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Cat. #	Product	Size	Price
631231	Lenti-X™ Concentrator	100 mL	USD $282.00
The Lenti-X Concentrator is a complete reagent for the concentration of infectious lentiviral vector particles. This reagent provides a scalable alternative to ultracentrifugation for viral particle concentration. Vector supernatants can be concentrated 10–100 fold, depending upon the volumes used. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Resources Back Rapid lentivirus concentration Rapid lentivirus concentration. Add Lenti-X Concentrator reagent to clarified viral supernatant, incubate for 30 min to overnight at 4°C, and spin. That’s it. Back Lentiviral concentration with high yield Lentiviral concentration with high yield. Lentiviral supernatant from a pLVX-ZsGreen1 vector was concentrated from 3 ml down to 30 µl using the Lenti-X Concentrator Reagent. The titer before and after concentration were measured and confirmed that >90% of the lentivirus remained functional. Samples were titrated on HT1080 cells and analyzed by flow cytometry 48 hr post-transduction. Back Concentrate lentivirus from any volume or from any lentiviral titer Concentrate lentivirus from any volume or from any lentiviral titer. Lentiviral supernatant was diluted into 250 ml and then concentrated down to 2.5 ml using Lenti-X Concentrator (Panel A). 10-fold serial dilutions of a high-titer lentiviral supernatant (high, medium, and low) were concentrated from a volume of 10 ml down to 100 µl using the Lenti-X Concentrator Reagent (Panel B). Titrations were performed using HT1080 cells and flow cytometry 48 hr post-transduction. Back 631231: Lenti-X Concentrator
631232	Lenti-X™ Concentrator	500 mL	USD $969.00
The Lenti-X Concentrator is a complete reagent for the concentration of infectious lentiviral vector particles. This reagent provides a scalable alternative to ultracentrifugation for viral particle concentration. Vector supernatants can be concentrated 10–100 fold, depending upon the volumes used. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Rapid lentivirus concentration Rapid lentivirus concentration. Add Lenti-X Concentrator reagent to clarified viral supernatant, incubate for 30 min to overnight at 4°C, and spin. That’s it. Back Lentiviral concentration with high yield Lentiviral concentration with high yield. Lentiviral supernatant from a pLVX-ZsGreen1 vector was concentrated from 3 ml down to 30 µl using the Lenti-X Concentrator Reagent. The titer before and after concentration were measured and confirmed that >90% of the lentivirus remained functional. Samples were titrated on HT1080 cells and analyzed by flow cytometry 48 hr post-transduction. Back Concentrate lentivirus from any volume or from any lentiviral titer Concentrate lentivirus from any volume or from any lentiviral titer. Lentiviral supernatant was diluted into 250 ml and then concentrated down to 2.5 ml using Lenti-X Concentrator (Panel A). 10-fold serial dilutions of a high-titer lentiviral supernatant (high, medium, and low) were concentrated from a volume of 10 ml down to 100 µl using the Lenti-X Concentrator Reagent (Panel B). Titrations were performed using HT1080 cells and flow cytometry 48 hr post-transduction. Back 631232: Lenti-X Concentrator

Overview

Simple mix-and-spin protocol
Hassle-free and easily scaled up for large volumes
No ultracentrifugation required
100X concentration
90% yield
Lenti-X Concentrator can also concentrate MMLV and MSCV retrovirus supernatants utilizing the longer incubation times recommended in the Retro-X Concentrator Protocol-At-A-Glance

More Information

Applications

Lentiviral transduction studies
Increased multiplicity of infection (MOI)
Rescue of low-titer supernatants

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Lenti-X Concentrator video

Lenti-X Concentrator is a simple and fast method for concentrating any lentiviral stock—just mix your lentiviral supernatant with the concentration reagent, incubate, and spin the mixture in a standard centrifuge. Watch the video to see how it works.

Watch Concentrator video Lentiviral packaging overview

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Concentrate lentiviral supernatants without ultracentrifugation

100-fold increase in titer

Far simpler than ultracentrifugation

Scalable for supernatants of any volume, any titer

Notice to purchaser

Rapid lentivirus concentration

Lentiviral concentration with high yield

Concentrate lentivirus from any volume or from any lentiviral titer

631231: Lenti-X Concentrator