Bacterial expression overview

Technologies

Takara Bio offers a choice of technologies for bacterial protein expression: standard E. coli expression, high-yield expression, low-temperature expression, high-purity expression, and bacterial expression systems.

Learn how to choose the best bacterial expression system for your specific application from our selection of standard E. coli expression systems, as well as specialized systems in E. coli or other bacterial hosts that are designed for high-yield, low-temperature, or high-purity expression.

Selection guide for bacterial expression systems
	Advantages	Protein yield per volume of culture	Applications
*Standard E. coli* expression systems**
pET Express & Purify Kits	High-yield, inducible, pET-based protein expression in N- or C-terminal 6xHN-tagged vectors Complete expression and purification system with a choice of In-Fusion or standard cloning options and TALON or His60 Ni purification resin/resin formats and buffers	Up to 1 g/l	Inducible expression and purification of his-tagged proteins
HAT Protein Expression & Purification System	Improved solubility of expressed proteins Native or denaturing purification of proteins at neutral pH, with included TALON resin, columns, and buffers	Up to 1 g/l	Expression and purification of HAT-tagged proteins
High-yield expression systems
B. subtilis Secretory Protein Expression System	Direct secretion of protein into culture medium In-Fusion Cloning-compatible systems	Up to 25 g/l	Fermentation of secreted proteins (medium- and large-scale)
Low-temperature expression system
pCold DNA cold shock expression system	Increased purity and yield resulting from low-temperature protein synthesis in E. coli that reduces expression of other proteins and protease activity Improved solubility of expressed proteins and increased recovery of soluble protein with the Chaperone Plasmid Set	Several mg to several 10s of mg/l	Expression of insoluble proteins and his-tagged proteins (with most of the pCold vectors) in E. coli
High-purity expression system
SPP System (single-protein rroduction system)	Higher purity due to highly specific expression of target protein in E. coli Efficient radioisotope labeling (up to 90% of newly expressed cellular protein)	Several 10s of mg/l	Expression of highly pure proteins, radioisotope labeling in E. coli

Cloning shouldn't be work, it should just work ...

With In-Fusion technology, you will be able to perform directional, seamless cloning of any PCR fragment—or multiple fragments—into any linearized vector in a single 15-minute reaction, with >95% cloning efficiency. Our In-Fusion bundles are complete systems that include all of the reagents you need for your cloning experiments: PCR enzyme, competent cells, and a kit for PCR cleanup or gel extraction. We have optimized the reagents to work together to give you the right clone the first time.

In-Fusion Snap Assembly

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