Magnetic bead-based isolation of genomic DNA from tissue—NucleoMag Tissue
NucleoMag Tissue is designed for manual or automated small-scale preparation of genomic DNA from human and animal tissue, cultured cells, or bacteria, in a 96-well format. After lysis of the sample, genomic DNA is selectively bound to NucleoMag Beads. Contaminants are washed away and the DNA can be eluted from the beads under low-salt conditions.
NucleoMag Tissue is designed for manual or automated small-scale preparation of genomic DNA from human and animal tissue, cultured cells, or bacteria, in a 96-well format. After lysis of the sample, genomic DNA is selectively bound to NucleoMag Beads. Contaminants are washed away and the DNA can be eluted from the beads under low-salt conditions. Since the magnetic bead technology provides a closed system (no transfer of sample from one well to another during the preparation), the risk for cross contamination is reduced.
Typically, 96 samples can be purified in less than 120 minutes on common automation platforms. The kit provides reagents for the purification of 10–20 µg of genomic DNA from samples (e.g., 20 mg of tissue or 107 cells). The purified DNA is ready to use for PCR, Southern blotting, and any enzymatic reaction.
Overview
- Minimize cross-contamination with a single-tube procedure
- Small elution volumes—as low as 50 µl
- Suitable for manual and automated processing
- Flexible—complete processing possible at room temperature (excluding lysis step)
More Information
| Technology | Magnetic bead technology |
| Format | Highly reactive superparamagnetic beads (size of beads: 1–3 µm) |
| Processing | Manual or automated |
| Starting material | <20 mg tissue, <107 cells or bacteria |
| Fragment size | 300 bp–>30 kb |
| Typical yield | 10–20 µg |
| A260/280 | >1.6 to 1.9 |
| Elution volume | >50 µl |
| Preparation time | 120 min/96 preps |
| Binding capacity | 0.4 µg/1 µl beads |
Applications
- Manual or automated isolation of DNA from human and animal tissue, cultured cells, or bacteria
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Detection of influenza A virus subtype H1N1
In this study, we describe the automated use of the NucleoMag Virus kit on the KingFisher Flex magnetic separator for the isolation of viral nucleic acids and subsequent detection by PCR. For kit evaluation, A/H1N1 samples from an interlaboratory test, as well as clinical samples, were used for kit evaluation. The data demonstrates the excellent quality of the eluted viral RNA which is suitable for downstream RT-PCR.
See the data Other articlesTakara Bio USA, Inc.
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