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  • ICELL8 cx Single-Cell System
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  • ICELL8 chips and consumables
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Home › Products › Next-generation sequencing › Single-cell NGS automation › ICELL8 cx chips and consumables

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ICELL8 cx chips and consumables

ICELL8 cx consumables

We offer a variety of accessories, plasticware, and other consumables for the ICELL8 cx Single-Cell System: plates, chips, chip loading and collection kits, etc. Use these consumables together with our validated ICELL8 cx workflows or your own custom workflows to generate NGS libraries for your single-cell applications.

We offer a variety of accessories, plasticware, and other consumables for the ICELL8 cx Single-Cell System: plates, chips, chip loading & collection kits, etc. Use these consumables together with our validated ICELL8 cx workflows or your own custom workflows to generate NGS libraries for your single-cell applications.

As part of the open design of the platform, we also offer a variety of blank chips containing no preprinted oligos for the development of custom applications. Blank chips can also be used to test cell dispensing and imaging using the ICELL8 cx system. Two depth options are available to accommodate different reaction volumes. In the chip name, the number denotes the volume of the nanowells while the letter(s) designation following the number indicates the shape of the well. (See table titled "Formats of available ICELL8 cx blank chips" under More Information.)

  • ICELL8 cx Blank Chip - 250 nl FB
  • ICELL8 350v Chip

The following products are required for processing ICELL8 cx chips:

  • The ICELL8 cx Loading Kit contains films and blotting paper designed to be used in the processing of ICELL8 cx chips
  • The ICELL8 Loading Kit – B contains blotting paper, optical film, and sealing film to be used for processing ICELL8 chips in workflows with multiple incubation steps
  • The ICELL8 Collection Kit – L contains a collection fixture to hold the chip, 2.0-ml tubes, and a film to allow users to spin down and collect the contents of a processed ICELL8 cx chip (e.g., extracted cDNA) into a tube for experiments

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Cat. # Product Size License Quantity Details
640197 ICELL8® cx Loading Kit Each USD $102.00

The ICELL8 cx Loading Kit includes Blotting Paper along with SmartChip films. This kit supports NGS applications on the ICELL8 cx Single-Cell System.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components

Required Products

Cat. # Product Size Price License Quantity Details
640000 ICELL8® Single-Cell System Each Inquire for Quotation

License Statement

ID Number  
329 This product is protected by one or more of these U.S. Patents 9,132,427, 9,909,171, 9,447,925, 9,951,381 and/or corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.
*

The ICELL8 Single-Cell System delivers unparalleled confidence and control for single-cell analysis by enabling precise cell isolation, cell selection, and high sample throughput. The system includes an ICELL8 MultiSample NanoDispenser, a complete imaging system—including microscope, computer, and software—a thermal cycler that accomodates ICELL8 chips, and additional accessories.

Documents Components Image Data

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Isolate nuclei and cells of any type

Isolate nuclei and cells of any type

The ICELL8 MSND's large-bore nozzle allows unbiased isolation of any sample. A few of the cell types that have been isolated using the system are listed. For samples highlighted in blue, the ICELL8 Single-Cell System is the first to allow successful isolation of single cells, nuclei, or spheroids.

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Bioanalyzer traces from example TCR libraries

Bioanalyzer traces from example TCR libraries

Electropherogram profiles of TCR sequencing libraries. Electropherograms from three different cell pools (see technical note for more details): Pool 9 (Panel A), 10 (Panel B), and 11 (Panel C). The variation in the profiles reflects the abundance of amplified TCRa (peak at ~900 bp) and TCRb (peak at ~700 bp). Panel D. Positive Control RNA TCR library (generated with 8 x 5 pg Control Jurkat Total RNA). Panel E. Negative Control TCR library shows no library is produced.

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ICELL8 system overview

ICELL8 system overview

Cell dispensing and visualization. After the ICELL8 MutliSample NanoDispenser has dispensed cells into the 5,184-nanowell chip, every well is imaged. Captured images are then processed in the CellSelect software to determine whether wells are empty, contain one cell, or contain multiple cells. This data can then be used to dispense reagents into the single-cell-containing wells.

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640000: ICELL8 Single-Cell System

640000: ICELL8 Single-Cell System
640194 ICELL8® cx Accessory Kit Each USD $2568.00

License Statement

ID Number  
325 Patent pending. For further information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.

The ICELL8 cx Accessory Kit is a component of the ICELL8 cx Single-Cell System and provides the accessories needed to run next-generation sequencing protocols related to ICELL8 cx applications, such as cDNA synthesis and PCR amplification.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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640194: ICELL8 cx Accessory Kit

640194: ICELL8 cx Accessory Kit
640192 ICELL8® 384-Well Source Plate and Seal Each USD $188.00

The ICELL8 384-Well Source Plate and Seal set is a component of the ICELL8 cx Single-Cell System. It includes NUNC plates and seals for covering the plates, which are utilized for sample and reagent dispense into SmartChip plates for related NGS applications.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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640192: ICELL8 384-Well Source Plate and Seal

640192: ICELL8 384-Well Source Plate and Seal
640018 MSND 384-Well Source Plate and Seals (430-000025) 20/Pack USD $482.00

20 Pack of MSND 384-Well Source Plate and Seals. Formerly 430-000025.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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640018: MSND 384-Well Source Plate and Seals (430-000025)

640018: MSND 384-Well Source Plate and Seals (430-000025)
640037 MSND 384-well Source Plate and Seals (430-000258) 120/Pack USD $1782.00

120 pack of MSND 384-well Source Plate and Seals. Formerly 430-000258.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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640037: MSND 384-well Source Plate and Seals (430-000258)

640037: MSND 384-well Source Plate and Seals (430-000258)
640206 ICELL8® Loading Kit – B Each USD $102.00

The ICELL8 Loading Kit – B contains blotting paper, optical film, and sealing film to be used for processing ICELL8 chips in workflows with multiple incubation steps.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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640206: ICELL8 Loading Kit - B

640206: ICELL8 Loading Kit - B
640212 ICELL8® Collection Kit – L Each USD $38.00

The ICELL8 Collection Kit – L consists of a collection fixture, 2.0-ml tubes, and collection film. This kit can be used to spin down a processed ICELL8 chip to collect the contents (e.g., cDNA or library product) of the chip into a tube, when the total extraction volume from the chip will be larger than 0.5 ml.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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640212: ICELL8 Collection Kit - L

640212: ICELL8 Collection Kit - L
640019 ICELL8® 350v Chip Each USD $749.00

The ICELL8 350v Chip is a 350-nl volume, V-bottom chip that enables users to dispense and image cells using ICELL8 single-cell systems. All wells in the 5,184-nanowell chip are unprinted and have a maximum working volume of 350 nl and a depth of 2.200 mm. Cells of choice are diluted, as per the appropriate application-specific ICELL8 or ICELL8 cx system user manual, and then dispensed and/or imaged on the ICELL8 or ICELL8 cx system. CellSelect Software is used to image and detect single-cell-bearing wells.

The ICELL8 350v Chip is used in the SMART-Seq ICELL8 cx application kit protocol along with the SMART-Seq ICELL8 Reagent Kit, which enables users to analyze full-length transcripts using the ICELL8 cx Single-Cell System.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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640019: ICELL8 350v Chip

640019: ICELL8 350v Chip
640193 ICELL8® cx Blank Chip - 250 nl FB 1 Chip USD $589.00

The ICELL8 cx Blank Chip - 250 nl FB is a 250-nl volume, flat-bottom chip which enables users to test cell dispense and cell imaging using the ICELL8 cx platform. All wells in the 5,184-nanowell chip are unprinted and have a maximum working volume of 200 nl and a depth of 1.600 mm. Cells of choice are diluted as per the appropriate assay-specific ICELL8 cx User Manual and dispensed and/or imaged on the ICELL8 cx system. ICELL8 cx CellSelect Software is used to image and detect single-cell-bearing wells.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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640193: ICELL8 cx Blank Chip - 250 nl FB

640193: ICELL8 cx Blank Chip - 250 nl FB
640258 SMART-Seq® Pro Indexing Primer Set - A 1 Chip USD $950.00

The SMART-Seq Pro Indexing Primer Set - A enables users to analyze full-length transcripts using ICELL8 single-cell systems and can generate indexed libraries that are ready for sequencing on Illumina platforms.

The plate contains 72 forward and 72 reverse indexes and a plate seal to process samples on one ICELL8 250v Chip or one ICELL8 350v Chip. This plate has been made available separately for customized protocol development. Primers are not resuspended to maximize flexibility with volumes and concentrations for assay developers.

This set contains different index primers than the SMART-Seq Pro Indexing Primer Set - B plate (Cat. # 640260). The SMART-Seq Pro protocol allows for the option to multiplex two libraries in a single sequencing run, one generated using Set A indexes and one generated using Set B indexes.

NOTE: This plate is already included with SMART-Seq Pro Application Kit - 2 Chip (Cat. No. 640257) and required for that workflow.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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640258: SMART-Seq Pro Indexing Primer Set - A

640258:  SMART-Seq Pro Indexing Primer Set - A
640260 SMART-Seq® Pro Indexing Primer Set - B 1 Chip USD $950.00

The SMART-Seq Pro Indexing Primer Set - B enables users to analyze full-length transcripts using ICELL8 single-cell systems and can generate indexed libraries that are ready for sequencing on Illumina platforms. 

The plate contains 72 forward and 72 reverse indexes and a plate seal to process samples on one ICELL8 250v Chip or one ICELL8 350v Chip. This plate has been made available separately for customized protocol development. Primers are not resuspended to maximize flexibility with volumes and concentrations for assay developers.

This set contains different index primers than the SMART-Seq Pro Indexing Primer Set - A plate (Cat. # 640258).  The SMART-Seq Pro protocol allows for the option to multiplex two libraries in a single sequencing run, one generated using Set A indexes and one generated using Set B indexes.

NOTE: This plate is already included with SMART-Seq Pro Application Kit - 2 Chip (Cat. No. 640257) and required for that workflow.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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640260: SMART-Seq Pro Indexing Primer Set - B

640260: SMART-Seq Pro Indexing Primer Set - B

ICELL8 sample prep guides ICELL8 sample prep protocols
Reagent preparation tips ICELL8 cx reagent & dispense guidelines

Overview

5,184-nanowell chip for single-cell analysis

ICELL8 cx chip shown with pipette tip for scale

Back side of ICELL8 cx chip showing serial number

More Information

Formats of available ICELL8 cx blank chips 

ChipVolume (nl)Depth (mm)Shape
ICELL8 cx Blank Chip - 250 nl FB 250 1.600 Flat bottom
ICELL8 350v Chip 350 2.200 V bottom

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


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