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  • ‹ Back to Next-generation sequencing
  • Harnessing the power of full-length transcriptome analysis for biomarker discoveries
  • Uncover more genes and SNPs from single cells
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Uncover more genes and SNPs from single cells with highly sensitive RNA-seq technology

With the single-cell data generated using our newest RNA-seq kits, you can gain deeper insights into single-cell biology:

  • Full-length chemistry powered by highly optimized enzymes
  • Unparalleled sensitivity & reproducibility with single-tube workflow
  • High library yields for easy adaptation to high-throughput sequencers
  • Validated with quality-control data you can rely upon

We have designed the plate-based SMART-Seq Single Cell PLUS Kit (SSsc PLUS kit) and automated SMART-Seq Pro Application Kit for the ICELL8 cx system (SS Pro kit) to generate the most data from your precious samples.

The SSsc PLUS kit gives you high-quality, full-length cDNA directly from single cells. It identifies more genes and generates a higher yield of Illumina-ready sequencing libraries than popular homebrew methods (Figure 1, Panels A and B).

The SS Pro kit is an automated, end-to-end solution for full-length single-cell RNA-seq. This kit allows you to generate libraries on a nanoliter scale for 1,500 single cells per experiment. In comparison studies, SS Pro kit detected more SNPs than end-counting technologies, such as 10x Chromium, even at low allele frequencies (Figure 1, Panel C).


Figure 1. SSsc PLUS and SS Pro kits enable the discovery of more genes and SNPs compared to popular scRNA-seq methods. Panel A. SSsc PLUS kit detects at least 10% more genes than the popular homebrew methods, Smart-seq2 (SS2) and Smart-seq3 (SS3), enabling detection of rare genes or genes previously missed. Panel B. SSsc PLUS kit allows researchers to input at least 8X more cDNA than the Nextera XT kit, which leads to higher library yields. Panel C. Comparison data shows that the SS Pro kit detects a greater number of SNPs than 10x Chromium across the board, particularly at lower allele frequencies (where data is more difficult to detect).


Easy-to-use data analysis software and tools for biomarker investigation

The SMART-Seq Pro application kit for the ICELL8 cx system is an end-to-end solution that includes analysis tools for full-length interrogation. The Cogent NGS Analysis Pipeline (CogentAP) generates visual map reports from NGS sequencing data, including percent mapped, unmapped, exonic, intronic, mitochondrial, and gDNA. This powerful bioinformatics analysis tool handles the majority of computation and performs genome- and transcript-based analyses.

Cogent NGS Discovery Software (CogentDS) is a GUI-based discovery tool for interactive analysis, such as auto-clustering, UMAP generation, and gene discovery (Figure 2).

Figure 2: The SMART-Seq Pro Application Kit enables key biomarker insights using included CogentDS tools. CogentDS has the ability to examine particular gene fusions of interest and overlay information from these fusions over generated UMAP plots. In this figure, the CogentDS overlay feature is applied over UMAP results for a specific gene fusion of interest, ACTB-ACTG1. Cells containing the gene fusion are then highlighted visually for better characterization of clusters.


Try our highest-sensitivity RNA-seq solutions and gain deeper insights into single-cell biology.


Cat. # Product Size Price License Quantity Details
R400750 SMART-Seq® Single Cell PLUS Kit 48 Rxns USD $4032.00

License Statement

ID Number  
385 This product is protected by U.S. Patents 7,803,550, 8,399,199, 8,728,737, and 9,598,727 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.
275 SMART-Seq2 Technology. This product is sold under exclusive license from Ludwig Institute of Cancer Research, Ltd. and is covered by US Patent No. 10266894, Japanese Patent No. 6336080, and European Patent No. 3036336, and pending U.S. patent application and/or pending claims of foreign counterparts. For license information, please contact a Takara Bio USA, Inc. licensing representative by e-mail at licensing@takarabio.com.

The SMART-Seq Single Cell PLUS Kit is a complete kit designed to first generate high-quality cDNA from single cells and then high-quality Illumina sequencing-ready libraries. Indexes are added using a unique dual index kit (Cat. # R400744 or R400745). This kit supports up to 48 reactions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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R400750: SMART-Seq Single Cell PLUS Kit

R400750: SMART-Seq Single Cell PLUS Kit

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The SMART-Seq Single Cell PLUS Kit demonstrates greater discrimination between control and single-cell samples than NEBNext.

The SMART-Seq Single Cell PLUS Kit demonstrates greater discrimination between control and single-cell samples than NEBNext.

Clustering of the SSsc single cell and positive controls and significantly distinct discrimination of the negative controls provides confidence in the data generated with SSsc PLUS. By UMAP analysis, there is a clear separation between the negative controls and the positive controls/single-cell samples for SSsc PLUS. As expected, the positive controls (grey) and the single-cell samples (green) cluster together for the SSsc PLUS while the negative controls (dusty blue) cluster together near the bottom of the chart. Interestingly, the SSsc PLUS negative controls and all of the NEBNext samples cluster together: negative (red), positive (light blue), and the single cells (purple). These analyses indicate that experimental noise, even when working with such small amounts of starting material, will not affect confidence in the biological import of the results. Data is shown for SMART-Seq Single Cell PLUS (SSsc PLUS). SMART-Seq mRNA Single Cell LP is an equivalent replacement for SSsc PLUS.

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Automation and miniaturization of cDNA synthesis on the mosquito HV.

Automation and miniaturization of cDNA synthesis on the mosquito HV.

Comparing full-volume versus eighth-volume processing on the mosquito HV with single CHO cells. Boxplots of gene counts for each preparation (medians: FV=16,592, Eighth=16,014). Boxplots show similar sensitivity for FV versus miniaturized volumes. All boxplots interquartile range (IQR) is the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data. Data is shown for SMART-Seq Single Cell (SSsc). SMART-Seq mRNA Single Cell is an equivalent replacement for SSsc.

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Automation and miniaturization of SMART-Seq mRNA Single Cell on the MANTIS Liquid Dispenser.

Automation and miniaturization of SMART-Seq mRNA Single Cell on the MANTIS Liquid Dispenser.

Comparing full-volume versus quarter-volume processing with single GM12878 cells on the MANTIS Liquid Dispenser. Boxplots of gene counts for each preparation (medians: FV=9,980, Quarter=9,603). Boxplots show similar sensitivity for FV versus miniaturized volumes. All boxplots interquartile range (IQR) is the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data. Data is shown for SMART-Seq Single Cell (SSsc). SMART-Seq mRNA Single Cell is an equivalent replacement for SMART-Seq Single Cell.

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Comparable data between SMART-Seq library prep and Nextera XT kits enable existing users to easily make the switch.

Comparable data between SMART-Seq library prep and Nextera XT kits enable existing users to easily make the switch.

Comparable data between SMART-Seq library prep and Nextera XT kits enable existing users to easily make the switch. Representative XY-plots of two example libraries from the same cDNA prepared with SSsc PLUS or Nextera XT in duplicate, and the same cDNA library compared between Nextera XT and PLUS. R2 values are shown in the graph; Nextera XT R2=0.9961, PLUS R2=0.9978, Nextera XT vs PLUS R2=0.9579. Data is shown for SMART-Seq Single Cell PLUS (SSsc PLUS). SMART-Seq mRNA Single Cell LP is an equivalent replacement for SSsc PLUS.

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Performance comparison of SS2 versus SSsc with GM12878 cells and SS3 versus SSsc with primary T cells isolated from PBMCs.

Performance comparison of SS2 versus SSsc with GM12878 cells and SS3 versus SSsc with primary T cells isolated from PBMCs.

Boxplots representing the distribution of gene counts for TPM > 0.1. The boxes denote the interquartile range (IQR), i.e., the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data. Outliers are plotted as empty circles. Data is shown for SMART-Seq Single Cell (SSsc). SMART-Seq mRNA Single Cell is an equivalent replacement for SSsc.

R400751 SMART-Seq® Single Cell PLUS Kit 96 Rxns USD $7390.00

License Statement

ID Number  
385 This product is protected by U.S. Patents 7,803,550, 8,399,199, 8,728,737, and 9,598,727 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.
275 SMART-Seq2 Technology. This product is sold under exclusive license from Ludwig Institute of Cancer Research, Ltd. and is covered by US Patent No. 10266894, Japanese Patent No. 6336080, and European Patent No. 3036336, and pending U.S. patent application and/or pending claims of foreign counterparts. For license information, please contact a Takara Bio USA, Inc. licensing representative by e-mail at licensing@takarabio.com.

The SMART-Seq Single Cell PLUS Kit is a complete kit designed to first generate high-quality cDNA from single cells and then high-quality Illumina sequencing-ready libraries. Indexes are added using a unique dual index kit (Cat. # R400744 or R400745). This kit supports up to 96 reactions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

The SMART-Seq Single Cell PLUS Kit demonstrates greater discrimination between control and single-cell samples than NEBNext.

The SMART-Seq Single Cell PLUS Kit demonstrates greater discrimination between control and single-cell samples than NEBNext.

Clustering of the SSsc single cell and positive controls and significantly distinct discrimination of the negative controls provides confidence in the data generated with SSsc PLUS. By UMAP analysis, there is a clear separation between the negative controls and the positive controls/single-cell samples for SSsc PLUS. As expected, the positive controls (grey) and the single-cell samples (green) cluster together for the SSsc PLUS while the negative controls (dusty blue) cluster together near the bottom of the chart. Interestingly, the SSsc PLUS negative controls and all of the NEBNext samples cluster together: negative (red), positive (light blue), and the single cells (purple). These analyses indicate that experimental noise, even when working with such small amounts of starting material, will not affect confidence in the biological import of the results. Data is shown for SMART-Seq Single Cell PLUS (SSsc PLUS). SMART-Seq mRNA Single Cell LP is an equivalent replacement for SSsc PLUS.

Back

Automation and miniaturization of cDNA synthesis on the mosquito HV.

Automation and miniaturization of cDNA synthesis on the mosquito HV.

Comparing full-volume versus eighth-volume processing on the mosquito HV with single CHO cells. Boxplots of gene counts for each preparation (medians: FV=16,592, Eighth=16,014). Boxplots show similar sensitivity for FV versus miniaturized volumes. All boxplots interquartile range (IQR) is the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data. Data is shown for SMART-Seq Single Cell (SSsc). SMART-Seq mRNA Single Cell is an equivalent replacement for SSsc.

Back

Automation and miniaturization of SMART-Seq mRNA Single Cell on the MANTIS Liquid Dispenser.

Automation and miniaturization of SMART-Seq mRNA Single Cell on the MANTIS Liquid Dispenser.

Comparing full-volume versus quarter-volume processing with single GM12878 cells on the MANTIS Liquid Dispenser. Boxplots of gene counts for each preparation (medians: FV=9,980, Quarter=9,603). Boxplots show similar sensitivity for FV versus miniaturized volumes. All boxplots interquartile range (IQR) is the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data. Data is shown for SMART-Seq Single Cell (SSsc). SMART-Seq mRNA Single Cell is an equivalent replacement for SMART-Seq Single Cell.

Back

Comparable data between SMART-Seq library prep and Nextera XT kits enable existing users to easily make the switch.

Comparable data between SMART-Seq library prep and Nextera XT kits enable existing users to easily make the switch.

Comparable data between SMART-Seq library prep and Nextera XT kits enable existing users to easily make the switch. Representative XY-plots of two example libraries from the same cDNA prepared with SSsc PLUS or Nextera XT in duplicate, and the same cDNA library compared between Nextera XT and PLUS. R2 values are shown in the graph; Nextera XT R2=0.9961, PLUS R2=0.9978, Nextera XT vs PLUS R2=0.9579. Data is shown for SMART-Seq Single Cell PLUS (SSsc PLUS). SMART-Seq mRNA Single Cell LP is an equivalent replacement for SSsc PLUS.

Back

Performance comparison of SS2 versus SSsc with GM12878 cells and SS3 versus SSsc with primary T cells isolated from PBMCs.

Performance comparison of SS2 versus SSsc with GM12878 cells and SS3 versus SSsc with primary T cells isolated from PBMCs.

Boxplots representing the distribution of gene counts for TPM > 0.1. The boxes denote the interquartile range (IQR), i.e., the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data. Outliers are plotted as empty circles. Data is shown for SMART-Seq Single Cell (SSsc). SMART-Seq mRNA Single Cell is an equivalent replacement for SSsc.

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R400751: SMART-Seq Single Cell PLUS Kit

R400751: SMART-Seq Single Cell PLUS Kit
640257 SMART-Seq® Pro Application Kit - 2 Chip 2 Chips USD $5214.00

License Statement

ID Number  
275 SMART-Seq2 Technology. This product is sold under exclusive license from Ludwig Institute of Cancer Research, Ltd. and is covered by US Patent No. 10266894, Japanese Patent No. 6336080, and European Patent No. 3036336, and pending U.S. patent application and/or pending claims of foreign counterparts. For license information, please contact a Takara Bio USA, Inc. licensing representative by e-mail at licensing@takarabio.com.

SMART-Seq Pro Application kit contains the necessary chips, reagents, and consumables required to run two complete experiments of single-cell, full-length transcriptome analysis on the ICELL8 cx Single-Cell System (Cat. No. 640188).

The protocols for performing SMART-Seq chemistry on ICELL8 systems leverage SMART technology for full-length cDNA synthesis and Nextera® technology for Illumina library preparation with dual 72 x 72 indexes (Nextera Tagmentation bead-linked transposome not included). The protocols generate indexed libraries that are ready for sequencing on Illumina platforms. Provided index plates allow for two chip libraries to be processed simultaneously in a single sequencer run.

ICELL8/MSND 384-Well Source Plate and Seals (Cat. Nos. 640192, 640018, or 640037) need to be purchased separately. A total of four plates and seals are required to process each individual chip.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

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Comparison of genes detected from PBMCs using SMART-Seq Pro or Smart-seq2 (SS2) workflows.

Comparison of genes detected from PBMCs using SMART-Seq Pro or Smart-seq2 (SS2) workflows.

Comparison of genes detected from PBMCs using SMART-Seq Pro or Smart-seq2 (SS2) workflows.

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Comparison of isoform data generated using the SMART-Seq Pro kit or 10x Chromium.

Comparison of isoform data generated using the SMART-Seq Pro kit or 10x Chromium.

Comparison of isoform data generated using the SMART-Seq Pro kit or 10x Chromium. The SMART-Seq Pro method using the ICELL8 cx system can identify junction reads across exons 3, 4, 5, 6, and 7 and shows distinct profiles for PTPRC-201 and PTPRC-209 isoforms. Data generated from 10x Chromium end-counting methods could not identify junctions in these same exon regions, with peaks representing noise from misaligned reads.

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640257: SMART-Seq Pro Application Kit - 2 Chip

640257: SMART-Seq Pro Application Kit - 2 Chip

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Takara Bio

Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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