Uncover more genes and SNPs from single cells with highly sensitive RNA-seq technology
With the single-cell data generated using our newest RNA-seq kits, you can gain deeper insights into single-cell biology:
- Full-length chemistry powered by highly optimized enzymes
- Unparalleled sensitivity & reproducibility with single-tube workflow
- High library yields for easy adaptation to high-throughput sequencers
- Validated with quality-control data you can rely upon
We have designed the plate-based SMART-Seq Single Cell PLUS Kit (SSsc PLUS kit) and automated SMART-Seq Pro Application Kit for the ICELL8 cx system (SS Pro kit) to generate the most data from your precious samples.
The SSsc PLUS kit gives you high-quality, full-length cDNA directly from single cells. It identifies more genes and generates a higher yield of Illumina-ready sequencing libraries than popular homebrew methods (Figure 1, Panels A and B).
The SS Pro kit is an automated, end-to-end solution for full-length single-cell RNA-seq. This kit allows you to generate libraries on a nanoliter scale for 1,500 single cells per experiment. In comparison studies, SS Pro kit detected more SNPs than end-counting technologies, such as 10x Chromium, even at low allele frequencies (Figure 1, Panel C).
Figure 1. SSsc PLUS and SS Pro kits enable the discovery of more genes and SNPs compared to popular scRNA-seq methods. Panel A. SSsc PLUS kit detects at least 10% more genes than the popular homebrew methods, Smart-seq2 (SS2) and Smart-seq3 (SS3), enabling detection of rare genes or genes previously missed. Panel B. SSsc PLUS kit allows researchers to input at least 8X more cDNA than the Nextera XT kit, which leads to higher library yields. Panel C. Comparison data shows that the SS Pro kit detects a greater number of SNPs than 10x Chromium across the board, particularly at lower allele frequencies (where data is more difficult to detect).
Easy-to-use data analysis software and tools for biomarker investigation
The SMART-Seq Pro application kit for the ICELL8 cx system is an end-to-end solution that includes analysis tools for full-length interrogation. The Cogent NGS Analysis Pipeline (CogentAP) generates visual map reports from NGS sequencing data, including percent mapped, unmapped, exonic, intronic, mitochondrial, and gDNA. This powerful bioinformatics analysis tool handles the majority of computation and performs genome- and transcript-based analyses.
Cogent NGS Discovery Software (CogentDS) is a GUI-based discovery tool for interactive analysis, such as auto-clustering, UMAP generation, and gene discovery (Figure 2).
Figure 2: The SMART-Seq Pro Application Kit enables key biomarker insights using included CogentDS tools. CogentDS has the ability to examine particular gene fusions of interest and overlay information from these fusions over generated UMAP plots. In this figure, the CogentDS overlay feature is applied over UMAP results for a specific gene fusion of interest, ACTB-ACTG1. Cells containing the gene fusion are then highlighted visually for better characterization of clusters.
Try our highest-sensitivity RNA-seq solutions and gain deeper insights into single-cell biology.
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