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Vaccine-grade plasmid DNA purification—NucleoBond RS

NucleoBond RS columns enable large-scale isolation of vaccine-grade, endotoxin-free plasmid DNA from bacterial cultures utilizing peristaltic pumps or FPLC systems. The NucleoBond RS workflow achieves endotoxin levels ≤0.01 EU/μg DNA, ensuring compliance for processes with high regulatory standards such as mRNA-based vaccine production. After two efficient washing steps that remove endotoxins and other contaminants, plasmid DNA is eluted, precipitated, and easily reconstituted in H2O-EF, TE-EF, or any other suitable endotoxin-free buffer for further use. In addition, RS columns are suitable for preparative scale RNA Clean-up from pre-purified RNA or IVT reactions.

NucleoBond RS columns enable large-scale isolation of vaccine-grade, endotoxin-free plasmid DNA from bacterial cultures utilizing peristaltic pumps or FPLC systems. The NucleoBond RS workflow achieves endotoxin levels ≤ 0.01 EU/μg DNA, ensuring compliance for processes with high regulatory standards such as mRNA-based vaccine production. After two efficient washing steps that remove endotoxins and other contaminants, plasmid DNA is eluted, precipitated, and easily reconstituted in H2O-EF, TE-EF, or any other suitable endotoxin-free buffer for further use. In addition, RS columns are suitable for preparative scale RNA clean-up from pre-purified RNA or IVT reactions.

For maximum flexibility, all components needed for plasmid purification or RNA clean-up using NucleoBond RS columns are offered as standalone products. For more detailed information regarding equipment and quantities of individual components, please refer to recommended reagents and equipment below.

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Cat. # Product Size Price License Quantity Details
743502 NucleoBond® RS 10 5 Columns USD $520.00

NucleoBond RS columns are designed for large-scale isolation of endotoxin-free plasmid DNA from bacterial cultures using peristaltic pumps or FPLC systems. After two efficient washing steps to remove endotoxins and other contaminants, plasmid DNA is eluted, precipitated, and easily reconstituted in H2O-EF, TE-EF, or any other suitable endotoxin-free buffer for further use. RS columns are also suitable for preparative-scale RNA clean-up from pre-purified RNA or IVT reactions.

NucleoBond RS 10 columns bind up to 10 mg of plasmid DNA from up to 8 g (wet weight; ODVmax 3.0) of bacterial cells or up to 10 mg of post-IVT RNA.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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743504 NucleoBond® RS 100 1 Column USD $960.00

NucleoBond RS columns are designed for large-scale isolation of endotoxin-free plasmid DNA from bacterial cultures using peristaltic pumps or FPLC systems. After two efficient washing steps to remove endotoxins and other contaminants, plasmid DNA is eluted, precipitated, and easily reconstituted in H2O-EF, TE-EF, or any other suitable endotoxin-free buffer for further use. RS columns are also suitable for preparative-scale RNA clean-up from pre-purified RNA or IVT reactions.

NucleoBond RS 100 columns bind up to 100 mg of plasmid DNA from up to 80 g (wet weight; ODVmax 30) of bacterial cells or up to 75 mg post-IVT RNA.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data
743505 NucleoBond® RS 200 1 Column USD $1700.00

NucleoBond RS columns are designed for large-scale isolation of endotoxin-free plasmid DNA from bacterial cultures using peristaltic pumps or FPLC systems. After two efficient washing steps to remove endotoxins and other contaminants, plasmid DNA is eluted, precipitated, and easily reconstituted in H2O-EF, TE-EF, or any other suitable endotoxin-free buffer for further use. RS columns are also suitable for preparative-scale RNA clean-up from pre-purified RNA or IVT reactions.

NucleoBond RS 200 columns bind up to 200 mg of plasmid DNA from up to 145 g (wet weight; ODVmax 54) of bacterial cells or up to 140 mg post-IVT RNA.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data
743503 NucleoBond® RS 50 1 Column USD $498.00

NucleoBond RS columns are designed for large-scale isolation of endotoxin-free plasmid DNA from bacterial cultures using peristaltic pumps or FPLC systems. After two efficient washing steps to remove endotoxins and other contaminants, plasmid DNA is eluted, precipitated, and easily reconstituted in H2O-EF, TE-EF, or any other suitable endotoxin-free buffer for further use. RS columns are also suitable for preparative-scale RNA clean-up from pre-purified RNA or IVT reactions.

NucleoBond RS 50 columns bind up to 50 mg of plasmid DNA from up to 40 g (wet weight; ODVmax 15) of bacterial cells or up to 45 mg post-IVT RNA.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Buffers
Enzymes
Accessories

Overview

A modular system for vaccine-grade plasmid purification or post-IVT RNA clean-up

  • Achieve endotoxin levels ≤0.01 EU/μg DNA
  • Process plasmids or post-IVT RNA using closed columns compatible with peristaltic pumps or FPLC
  • Scale up from 10 mg to 200 mg bacterial culture (or 1 mg to 140 mg post-IVT RNA) with four column sizes

Recommended reagents for plasmid purification

Product Size Specification Cat. #
Buffer RES-EF 1,000 mL Resuspension buffer 740386.1000
Buffer LYS-EF 1,000 mL Lysis buffer 740387.1000
Buffer NEU-EF 1,000 mL Neutralization buffer 740388.1000
Buffer EQ-EF 1,000 mL
3,000 mL		 Equilibration buffer 740380.1000
740380.3000
Buffer BMC-EF* 1,000 mL
3,000 mL		 Binding mediating reconstitution buffer 740382.1000
740382.3000
Buffer ENTO-EF 1,000 mL
3,000 mL		 Endotoxin removal buffer 740381.1000
740381.3000
Buffer WASH-EF 1,000 mL Wash buffer 740392.1000
Buffer ELU-EF 900 mL Elution buffer 740393.900

*Buffer BMC-EF is needed for the alternative RNAse-free NucleoBond RS plasmid purification workflow. Please refer to the NucleoBond RS Endotoxin-Free Plasmid DNA Purification User Manual for more information.

Recommended reagents for post-IVT RNA clean-up

Product Size Specification Cat. #
Buffer NRB 1,000 mL
3,000 mL		 Binding buffer 740371.1000
740371.3000
Buffer EQU 1,000 mL Equilibration buffer 740317.1000
Buffer NRW 1,000 mL
3,000 mL		 Wash buffer 740372.1000
740372.3000
Buffer NRE 1,000 mL
3,000 mL		 Elution buffer 740373.1000
740373.3000

Additional recommended reagents and equipment

Product Size Specification Cat. #
Buffer TE-EF 1,000 mL Endotoxin-free TE buffer 740797.1
Buffer H2O-EF 1,000 mL Endotoxin-free water 740798.1
RNase A 50 mg
100 mg		 Enzymatic degradation and removal of RNA 740505.50
740505.100
NucleoBond Bottle Top Filter Type 2 5 Filters Lysate filtration 740553.5
NucleoVac 96 Vacuum Regulator 1 piece Vacuum manifold for controlling vacuum 740641

More Information

Specifications of plasmid DNA isolated using NucleoBond RS columns

Parameter Method Criterion
Integrity Agarose gel electrophoresis/fragment analyzer ≥90% supercoiled form
Purity UV spectrum A260/A280 1.8–2.0
A260/A230 1.9–2.3
Endotoxin levels LAL/rFC ≤0.01 EU/μg DNA
Host DNA gDNA PCR ≤1%
Host RNA Agarose gel electrophoresis
Fluorometric quantification		 Not visible at 200 ng DNA
≤2%
Host proteins BCA ≤1%

Applications

  • Large-scale, endotoxin-free plasmid purification
  • Preparation of vaccine-grade plasmid DNA
  • Post-IVT RNA clean-up

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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