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Photoactivatable mCherry fluorescent protein

Living Colors PAmCherry is a photoactivatable fluorescent protein (PAFP) derived from the red fluorescent protein mCherry. PAmCherry is non-fluorescent until it is exposed to 350- to 400-nm light. By selecting which cells or cellular regions to activate, you can track cells, organelles, or proteins of interest against a dark background using this photoactivatable fluorescent protein. The activated PAmCherry excitation maximum is 564 nm, and the emission maximum is 595 nm, which allows you to visualize and monitor photoactivated PAmCherry with the same filter sets used for other red fluorescent proteins, such as DsRed variants and mCherry.

Photoactivatable fluorescent proteins such as PAmCherry are particularly useful for determining protein half-life and protein transport pathways because molecules synthesized after activation are not able to fluoresce. Therefore, what you observe is a snapshot of the protein molecules that were present at the time of activation.

PAmCherry subcellular localization vectors are available that target PAmCherry to the cell membrane, mitochondria, actin, or tubulin, respectively. Plasmid and lentiviral N- and C-terminal vectors are also available to create your own PAmCherry-tagged protein of interest.

PAmCherry sequence files can be found in the Documents tab by expanding Details within the Product Table below.

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Cat. #	Product	Size	Price	Quantity
632591	pPAmCherry-Mito Vector	10 ug	Inquire for Quotation	*
pPAmCherry-Mito Vector is a mammalian expression vector encoding PAmCherry, a photoactivatable mutant of the fluorescent protein mCherry, fused to the mitochondrial targeting sequence derived from the precursor subunit VIII of human cytochrome C oxidase. PAmCherry is non-fluorescence until photoactivated by a short exposure to light at a wavelength between 350 nm and 400 nm. The excitation/emission wavelengths of photoactivated PAmCherry are 564 nm and 595 nm. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back Photoactivated PAmCherry-Mito shows strong red fluorescence and localizes correctly to the mitochondria Photoactivated PAmCherry-Mito shows strong red fluorescence and localizes correctly to the mitochondria. U2OS cells were transiently transfected with pPAmCherry-Mito. PAmCherry-Mito was activated using an Argon 458 nm laser (scan speed: 400 Hz), and the cells were imaged using a HeNe 543 nm laser for excitation and a standard red fluorescence emission filter set. Back The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells The mCherry monoclonal antibody detects PAmCherry-N1 in the lysate of mammalian cells. HEK 293 cells were transiently transfected with pPAmCherry-N1. Cell lysates (corresponding to 30,000 cells) were prepared from HEK 293 cells transiently expressing PAmCherry-N1 (Lane 1) or a negative control (untransfected cells; Lane 2). Both lysates and a positive control (5 ng recombinant mCherry; Lane 3) were separated by SDS-PAGE and analyzed by Western blot using the mCherry monoclonal antibody at the recommended dilution of 1:1000. Back Prior to photoactivation, no red fluorescence is detected in a cell expressing PAmCherry-Mito (Panel A) Prior to photoactivation, no red fluorescence is detected in a cell expressing PAmCherry-Mito (Panel A). However, after photoactivation, strong red fluorescence is observed in the subcellular, activated region of the cell (Panel B). U2OS cells were transiently transfected with pPAmCherry-Mito. Cells were imaged prior to activation in order to determine the level of background fluorescence (Panel A). PAmCherry-Mito was then activated in a small region of a cell imaged using a HeNe 543 nm laser for excitation and a standard red fluorescence emission filter set (Panel B). Back PAmCherry-Mito makes it easy to follow the behavior of a subset of mitochondria PAmCherry-Mito makes it easy to follow the behavior of a subset of mitochondria. Activating the mitochondria in just one region of the cell makes it possible to follow their movements into dark (nonactivated) areas of the cell. U2OS cells were transiently transfected with pPAmCherry-Mito. PAmCherry-Mito was activated in a small region of a cell, and the cells were imaged every 10 sec for 15 min using a HeNe 543 nm laser for excitation and a standard red fluorescence emission filter set. Back PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background. Visualize PAmCherry with the same filter sets you use to detect other red fluorescent proteins, such as DsRed and mCherry. Back 632591: pPAmCherry-Mito Vector
632590	pPAmCherry-Tubulin Vector	10 ug	Inquire for Quotation	*
pPAmCherry-Tubulin Vector is a mammalian expression vector encoding PAmCherry, a photoactivatable mutant of the fluorescent protein mCherry, fused to human alpha-tubulin. PAmCherry is non-fluorescence until photoactivated by a short exposure to light at a wavelength between 350 nm and 400 nm. The excitation/emission wavelengths of photoactivated PAmCherry are 564 nm and 595 nm. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back 632590: pPAmCherry-Tubulin Vector Back The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells The mCherry monoclonal antibody detects PAmCherry-N1 in the lysate of mammalian cells. HEK 293 cells were transiently transfected with pPAmCherry-N1. Cell lysates (corresponding to 30,000 cells) were prepared from HEK 293 cells transiently expressing PAmCherry-N1 (Lane 1) or a negative control (untransfected cells; Lane 2). Both lysates and a positive control (5 ng recombinant mCherry; Lane 3) were separated by SDS-PAGE and analyzed by Western blot using the mCherry monoclonal antibody at the recommended dilution of 1:1000. Back PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background. Visualize PAmCherry with the same filter sets you use to detect other red fluorescent proteins, such as DsRed and mCherry.
632589	pPAmCherry-Actin Vector	10 ug	Inquire for Quotation	*
pPAmCherry-Actin Vector is a mammalian expression vector encoding PAmCherry, a photoactivatable mutant of the fluorescent protein mCherry, fused to human cytoplasmic beta-actin. PAmCherry is non-fluorescence until photoactivated by a short exposure to light at a wavelength between 350 nm and 400 nm. The excitation/emission wavelengths of photoactivated PAmCherry are 564 nm and 595 nm. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back 632589: pPAmCherry-Actin Vector Back The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells The mCherry monoclonal antibody detects PAmCherry-N1 in the lysate of mammalian cells. HEK 293 cells were transiently transfected with pPAmCherry-N1. Cell lysates (corresponding to 30,000 cells) were prepared from HEK 293 cells transiently expressing PAmCherry-N1 (Lane 1) or a negative control (untransfected cells; Lane 2). Both lysates and a positive control (5 ng recombinant mCherry; Lane 3) were separated by SDS-PAGE and analyzed by Western blot using the mCherry monoclonal antibody at the recommended dilution of 1:1000. Back PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background. Visualize PAmCherry with the same filter sets you use to detect other red fluorescent proteins, such as DsRed and mCherry.
632588	pPAmCherry-Mem Vector	10 ug	Inquire for Quotation	*
pPAmCherry-Mem Vector is mammalian expression vector encoding a fusion protein of PAmCherry and the N-terminal 20 amino acids of neuromodulin (GAP-43). PAmCherry is a photoactivatable mutant of the fluorescent protein mCherry. PAmCherry is non-fluorescence until photoactivated by a short exposure to light at a wavelength between 350 nm and 400 nm. The excitation/emission wavelengths of photoactivated PAmCherry are 564 nm and 595 nm. The GAP-43 fragment contains a signal for posttranslational palmitoylation of cysteins 3 and 4 that targets the fusion protein to the plasma membrane. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back 632588: pPAmCherry-Mem Vector Back The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells The mCherry monoclonal antibody detects PAmCherry-N1 in the lysate of mammalian cells. HEK 293 cells were transiently transfected with pPAmCherry-N1. Cell lysates (corresponding to 30,000 cells) were prepared from HEK 293 cells transiently expressing PAmCherry-N1 (Lane 1) or a negative control (untransfected cells; Lane 2). Both lysates and a positive control (5 ng recombinant mCherry; Lane 3) were separated by SDS-PAGE and analyzed by Western blot using the mCherry monoclonal antibody at the recommended dilution of 1:1000. Back PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background. Visualize PAmCherry with the same filter sets you use to detect other red fluorescent proteins, such as DsRed and mCherry.
632584	pPAmCherry-N1 Vector	10 ug	Inquire for Quotation	*
pPAmCherry-N1 Vector is a mammalian expression vector encoding PAmCherry, a photoactivatable mutant of the fluorescent protein mCherry. PAmCherry is non-fluorescent until photoactivated by a short exposure to light at a wavelength between 350 nm and 400 nm. The excitation/emission wavelengths of photoactivated PAmCherry are 564 nm and 595 nm. Genes cloned into the MCS will be expressed as fusions to the N-terminus of PAmCherry if they are in the same reading frame as PAmCherry and there are no intervening stop codons. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells The mCherry monoclonal antibody detects PAmCherry-N1 in the lysate of mammalian cells. HEK 293 cells were transiently transfected with pPAmCherry-N1. Cell lysates (corresponding to 30,000 cells) were prepared from HEK 293 cells transiently expressing PAmCherry-N1 (Lane 1) or a negative control (untransfected cells; Lane 2). Both lysates and a positive control (5 ng recombinant mCherry; Lane 3) were separated by SDS-PAGE and analyzed by Western blot using the mCherry monoclonal antibody at the recommended dilution of 1:1000. Back PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background. Visualize PAmCherry with the same filter sets you use to detect other red fluorescent proteins, such as DsRed and mCherry.
632585	pPAmCherry-C1 Vector	10 ug	Inquire for Quotation	*
pPAmCherry-C1 Vector is a mammalian expression vector encoding PAmCherry, a photoactivatable mutant of the fluorescent protein mCherry. PAmCherry is non-fluorescent until photoactivated by a short exposure to light at a wavelength between 350 nm and 400 nm. The excitation/emission wavelengths of photoactivated PAmCherry are 564 nm and 595 nm. Genes cloned into the MCS will be expressed as fusions to the C-terminus of PAmCherry if they are in the same reading frame as PAmCherry and there are no intervening stop codons. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells The mCherry monoclonal antibody detects PAmCherry-N1 in the lysate of mammalian cells. HEK 293 cells were transiently transfected with pPAmCherry-N1. Cell lysates (corresponding to 30,000 cells) were prepared from HEK 293 cells transiently expressing PAmCherry-N1 (Lane 1) or a negative control (untransfected cells; Lane 2). Both lysates and a positive control (5 ng recombinant mCherry; Lane 3) were separated by SDS-PAGE and analyzed by Western blot using the mCherry monoclonal antibody at the recommended dilution of 1:1000. Back PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background. Visualize PAmCherry with the same filter sets you use to detect other red fluorescent proteins, such as DsRed and mCherry.
632586	pLVX-PAmCherry-N1 Vector	10 ug	Inquire for Quotation	*
pLVX-PAmCherry-N1 Vector is an HIV-1-based, lentiviral expression vector. Lentiviral particles derived from the vector allow you to infect cells and express your gene of interest fused to PAmCherry, a photoactivatable mutant of the fluorescent protein mCherry. PAmCherry is non-fluorescence until photoactivated by a short exposure to light at a wavelength between 350 nm and 400 nm. The excitation/emission wavelengths of photoactivated PAmCherry are 564 nm and 595 nm. Genes cloned into the MCS will be expressed as fusions to the N-terminus of PAmCherry if they are in the same reading frame as PAmCherry and there are no intervening stop codons. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells The mCherry monoclonal antibody detects PAmCherry-N1 in the lysate of mammalian cells. HEK 293 cells were transiently transfected with pPAmCherry-N1. Cell lysates (corresponding to 30,000 cells) were prepared from HEK 293 cells transiently expressing PAmCherry-N1 (Lane 1) or a negative control (untransfected cells; Lane 2). Both lysates and a positive control (5 ng recombinant mCherry; Lane 3) were separated by SDS-PAGE and analyzed by Western blot using the mCherry monoclonal antibody at the recommended dilution of 1:1000. Back PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background. Visualize PAmCherry with the same filter sets you use to detect other red fluorescent proteins, such as DsRed and mCherry.
632587	pLVX-PAmCherry-C1 Vector	10 ug	Inquire for Quotation	*
pLVX-PAmCherry-C1 Vector is an HIV-1-based, lentiviral expression vector. Lentiviral particles derived from the vector allow you to infect cells and express your gene of interest fused to PAmCherry, a photoactivatable mutant of the fluorescent protein mCherry. PAmCherry is non-fluorescent until photoactivated by a short exposure to light at a wavelength between 350 nm and 400 nm. The excitation/emission wavelengths of photoactivated PAmCherry are 564 nm and 595 nm. Genes cloned into the MCS will be expressed as fusions to the C-terminus of PAmCherry if they are in the same reading frame as PAmCherry and there are no intervening stop codons. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells The mCherry monoclonal antibody detects PAmCherry-N1 in the lysate of mammalian cells. HEK 293 cells were transiently transfected with pPAmCherry-N1. Cell lysates (corresponding to 30,000 cells) were prepared from HEK 293 cells transiently expressing PAmCherry-N1 (Lane 1) or a negative control (untransfected cells; Lane 2). Both lysates and a positive control (5 ng recombinant mCherry; Lane 3) were separated by SDS-PAGE and analyzed by Western blot using the mCherry monoclonal antibody at the recommended dilution of 1:1000. Back PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background. Visualize PAmCherry with the same filter sets you use to detect other red fluorescent proteins, such as DsRed and mCherry.

*You must be logged in to a Purchasing Account in order to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@takarabio.com.

Overview

Switch on the red fluorescent protein mCherry in a subset of cells, proteins, or organelles, in order to track their movement.

Non-fluorescent until activated by a short exposure to 350- to 400-nm light
(This is the optimal range of wavelengths)
Excitation maximum: 564 nm
Emission maximum: 595 nm
Image PAmCherry with the same filter sets used to detect red fluorescent proteins such as DsRed and mCherry
Detect PAmCherry with the mCherry monoclonal antibody

More Information

Applications

Tracking subpopulations of organelles or protein molecules
Visualizing real-time protein dynamics (movement, degradation, etc.)
Determining protein half-life and protein transport pathways
Observing cell division, migration, and morphology

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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Photoactivatable mCherry fluorescent protein

Notice to purchaser

Photoactivated PAmCherry-Mito shows strong red fluorescence and localizes correctly to the mitochondria

The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells

Prior to photoactivation, no red fluorescence is detected in a cell expressing PAmCherry-Mito (Panel A)

PAmCherry-Mito makes it easy to follow the behavior of a subset of mitochondria

PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background

632591: pPAmCherry-Mito Vector

Notice to purchaser

632590: pPAmCherry-Tubulin Vector

The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells

PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background

Notice to purchaser

632589: pPAmCherry-Actin Vector

The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells

PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background

Notice to purchaser

632588: pPAmCherry-Mem Vector

The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells

PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background

Notice to purchaser

The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells

PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background

Notice to purchaser

The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells

PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background

Notice to purchaser

The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells

PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background

Notice to purchaser

The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells

PAmCherry is easy to use: Activate PAmCherry in a specific cell or region of a cell, and then track your labeled and activated cells, organelles, or proteins of interest against a dark background

Overview

More Information

Applications

Additional product information

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