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AcGFP1 fluorescent protein

AcGFP1 (Aequorea coerulescens GFP) is a monomeric green fluorescent protein with spectral properties similar to those of EGFP. Although AcGFP1 and EGFP sequences have 94% homology at the amino acid level and equivalent brightness, AcGFP1 is a superior alternative for fusion applications because it is a monomer. In contrast, the Aequorea victoria GFP and EGFP form dimers (Jain et al. 2001, Chen et al. 2002). The monomeric nature of AcGFP1 has been confirmed by FPLC gel filtration chromatography, sucrose density gradient ultracentrifugation, and pseudonative gel electrophoresis. (Click the Image Data button in the product table below to view data.)

The AcGFP1 fluorescent protein is very stable, allowing you to perform studies over an extended period of time. AcGFP1 can be used to visualize and measure gene expression in a variety of organisms from bacteria to higher plants and animals.

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632515

pIRES2-AcGFP1-Nuc Vector

20 ug

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ID Number

Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pIRES2-AcGFP1-Nuc Vector product License Agreement can be found by clicking here.

AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.

pIRES2-AcGFP1-Nuc encodes AcGFP1 with three identical copies of the nuclear localization signal (NLS) of the simian virus 40 large T antigen fused in tandem to its C-terminus. The gene encoding the AcGFP1-Nuc fusion protein is located downstream of the IRES2 sequence. This permits both the gene of interest (cloned into the MCS upstream of the IRES2 sequence) and the AcGFP1-Nuc gene to be translated from a single bicistronic mRNA. AcGFP-1-Nuc localizes to the nucleus of a transfected cell, offering an advantage over random localization in the cytosol. It is most useful for those who are interested in monitoring translocation events in and out of the nucleus.

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Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Components

632511

pAcGFP1-F Vector

20 ug

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39	AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.

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632510

pAcGFP1-F Hyg Vector

20 ug

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39	AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.

pAcGFP1-F encodes a fusion protein consisting of the Aequorea coerulescens green fluorescent protein and the farnesylation signal of c-Ha-ras. AcGFP1 is a variant of the Aequorea coerulescens green fluorescent protein (AcGFP). The AcGFP1 coding sequence in this construct has been optimized for efficient expression and enhanced brightness in mammalian cells. pAcGFP1-F Hyg can be used for labeling the inner leaflet of the plasma membrane. The hygromycin resistance cassette allows you to create double-stable cell lines if this vector is used in conjunction with any other Living Colors vector that contains a neomycin resistance cassette.

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632509

pAcGFP1-Mem Hyg Vector

20 ug

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ID Number
39	AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.

pAcGFP1-Mem Hyg encodes a fusion protein consisting of the Aequorea coerulescens green fluorescent protein and the N-terminal membrane-targeting signal of neuromodulin (also called GAP-43). AcGFP1 is a variant of the Aequorea coerulescens green fluorescent protein (AcGFP) that has been optimized for brighter fluorescence and higher expression in mammalian cells. pAcGFP1-Mem Hyg can be used for labeling the plasma membrane. The hygromycin resistance cassette allows you to create double-stable cell lines if this vector is used in conjunction with any other Living Colors vector that contains a neomycin resistance cassette.

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Components

632501

pAcGFP1-N In-Fusion® Ready Vector

1 ug

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ID Number

Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pAcGFP1-N In-Fusion® Ready Vector product License Agreement can be found by clicking here.

AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.

pAcGFP1-N In-Fusion Ready Vector encodes a green fluorescent protein from Aequorea coerulescens. The vector is prelinearized to enable directional In-Fusion cloning of PCR fragments onto the 5' end of the AcGFP1-encoding gene without further restriction digests. Sequences complementary to the vector sequences (flanking the site of linearization) need to be incorporated at the 5' ends of the PCR primers (see PT3865-2). The gene of interest is expressed as an N-terminal fusion to AcGFP1 in mammalian cells. The fluorescent protein coding sequence in this construct has been human codon-optimized for efficient expression and enhanced brightness in mammalian cells.

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632500

pAcGFP1-C In-Fusion® Ready Vector

1 ug

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ID Number

Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pAcGFP1-C In-Fusion® Ready Vector product License Agreement can be found by clicking here.

AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.

pAcGFP1-C In-Fusion Ready Vector encodes a green fluorescent protein from Aequorea coerulescens. The vector is prelinearized to enable directional In-Fusion cloning of PCR fragments onto the 3' end of the AcGFP1-encoding gene without further restriction digests. Sequences complementary to the vector sequences (flanking the site of linearization) need to be incorporated at the 5' ends of the PCR primers (see PT3865-2). The gene of interest is expressed as a C-terminal fusion to AcGFP1 in mammalian cells. The fluorescent protein coding sequence in this construct has been human codon-optimized for efficient expression and enhanced brightness in mammalian cells.

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Components

632497

pAcGFP1-1 Vector

20 ug

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License Statement

ID Number

Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pAcGFP1-1 Vector product License Agreement can be found by clicking here.

AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.

pAcGFP1-1 encodes a green fluorescent protein (AcGFP1) from Aequorea coerulescens. The fluorescent protein coding sequence in this construct has been human-codon-optimized for efficient expression and enhanced brightness in mammalian cells. The vector contains a neomycin resistance gene for the selection of stable transfectants. pAcGFP1-1 can be used as a reporter to monitor the activity of promoters and promoter/enhancer combinations cloned into the multiple cloning site (MCS) upstream of the promoterless AcGFP1 gene. Without the addition of a functional promoter, this vector will not express AcGFP1.

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Components

632492

pAcGFP1-Hyg-C1 Vector

20 ug

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License Statement

ID Number

Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pAcGFP1-Hyg-C1 Vector product License Agreement can be found by clicking here.

AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.

pAcGFP1-Hyg-C1 encodes a green fluorescent protein (AcGFP1) from Aequorea coerulescens. pAcGFP1-Hyg-C1 is designed for studies in mammalian systems. This vector allows expression of a protein of interest as a C-terminal fusion to AcGFP1. The vector contains a hygromycin resistance gene for the selection of stable transfectants. pAcGFP1-Hyg-C1 can also be used as a cotransfection marker since the unmodified vector constitutively expresses AcGFP1. The fluorescent protein coding sequence in this construct has been human codon-optimized for efficient expression and enhanced brightness in mammalian cells.

Notice to purchaser

Components

632491

pAcGFP1-Mem Vector

20 ug

Inquire for Quotation

License Statement

ID Number

Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pAcGFP1-Mem Vector product License Agreement can be found by clicking here.

AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.

pAcGFP1-Mem encodes a fusion protein consisting of the Aequorea coerulescens green fluorescent protein and the N-terminal membrane-targeting signal of neuromodulin (also called GAP-43). AcGFP1 is a variant of the Aequorea coerulescens green fluorescent protein (AcGFP) that has been optimized for brighter fluorescence and higher expression in mammalian cells. pAcGFP1-Mem can be used for labeling the plasma membrane.

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Components

632490

pAcGFP1-Endo Vector

20 ug

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ID Number

Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pAcGFP1-Endo Vector product License Agreement can be found by clicking here.

AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.

pAcGFP1-Endo encodes a fusion protein consisting of the Aequorea coerulescens green fluorescent protein, c-Myc, and RhoB protein. The RhoB GTPase localizes the fusion protein to vesicles involved in endocytosis and allows monitoring of intracellular membrane traffic in living and fixed cells using fluorescence microscopy. The c-Myc epitope allows detection of the fusion protein using c-Myc antibodies. AcGFP1 is a variant of the Aequorea coerulescens green fluorescent protein (AcGFP) that has been optimized for brighter fluorescence and higher expression in mammalian cells.

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Components

632489

pAcGFP1-Hyg-N1 Vector

20 ug

Inquire for Quotation

License Statement

ID Number

Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pAcGFP1-Hyg-N1 Vector product License Agreement can be found by clicking here.

AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.

pAcGFP1-Hyg-N1 encodes a green fluorescent protein from Aequorea coerulescens. pAcGFP1-Hyg-N1 is designed for studies in mammalian systems. This vector allows expression of a protein of interest as an N-terminal fusion to AcGFP1. The vector contains a hygromycin resistance gene for the selection of stable transfectants. The fusion vector can also be used as a cotransfection marker since the unmodified vector constitutively expresses fluorescent protein. The fluorescent protein coding sequence in this construct has been human codon-optimized for efficient expression and enhanced brightness in mammalian cells.

Notice to purchaser

Components

632488

pAcGFP1-Tubulin Vector

20 ug

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License Statement

ID Number

Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pAcGFP1-Tubulin Vector product License Agreement can be found by clicking here.

AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.

pAcGFP1-Tubulin encodes a fusion protein consisting of the AcGFP1 green fluorescent protein (GFP) and human a-tubulin. AcGFP1 is an Aequorea coerulescens GFP that has been optimized for brighter fluorescence and higher expression in mammalian cells. The AcGFP1-Tubulin fusion protein incorporates into growing microtubules, allowing visualization of the microtubule network in living or fixed cells.

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Components

632485

pAcGFP1-N Vector Set

Each

Inquire for Quotation

License Statement

ID Number

Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pAcGFP1-N Vector Set product License Agreement can be found by clicking here.

AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.

A set of three vectors encoding a green fluorescent protein from Aequorea coerulescens. The pAcGFP1-N vectors are designed for studies in mammalian systems. These vectors allow expression of a protein of interest as a N-terminal fusion. Each vector contains the MCS in a different reading frame with respect to AcGFP1. These fusion vectors can also be used as cotransfection markers since the unmodified vectors will express fluorescent protein. All of the fluorescent protein coding sequences in these constructs have been human-codon-optimized for efficient expression in mammalian cells.

Notice to purchaser

Components

632484

pAcGFP1-N3 Vector

20 ug

Inquire for Quotation

License Statement

ID Number

Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pAcGFP1-N3 Vector product License Agreement can be found by clicking here.

AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.

pAcGFP1-N3 encodes a green fluorescent protein from Aequorea coerulescens. pAcGFP1-N3 is designed for studies in mammalian systems. This vector allows expression of a protein of interest as an N-terminal fusion to AcGFP1. The fusion vector can also be used as a cotransfection marker since the unmodified vector will express fluorescent protein. The fluorescent protein coding sequence in this construct has been human-codon-optimized for efficient expression and enhanced brightness in mammalian cells.

Notice to purchaser

Components

632483

pAcGFP1-N2 Vector

20 ug

Inquire for Quotation

License Statement

ID Number

Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pAcGFP1-N2 Vector product License Agreement can be found by clicking here.

AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.

pAcGFP1-N2 encodes a green fluorescent protein from Aequorea coerulescens. pAcGFP1-N2 is designed for studies in mammalian systems. This vector allows expression of a protein of interest as an N-terminal fusion to AcGFP1. The fusion vector can also be used as a cotransfection marker since the unmodified vector will express fluorescent protein. The fluorescent protein coding sequence in this construct has been human-codon-optimized for efficient expression and enhanced brightness in mammalian cells.

Notice to purchaser

Components

632482

pAcGFP1-C3 Vector

20 ug

Inquire for Quotation

License Statement

ID Number

Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pAcGFP1-C3 Vector product License Agreement can be found by clicking here.

AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.

pAcGFP1-C3 encodes a green fluorescent protein from Aequorea coerulescens. pAcGFP1-C3 is designed for studies in mammalian systems. This vector allows expression of a protein of interest as a C-terminal fusion to AcGFP1. The fusion vector can also be used as a cotransfection marker since the unmodified vector will express fluorescent protein. The fluorescent protein coding sequence in this construct has been human-codon-optimized for efficient expression and enhanced brightness in mammalian cells.

Notice to purchaser

Components

632481

pAcGFP1-C2 Vector

20 ug

Inquire for Quotation

License Statement

ID Number

Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pAcGFP1-C2 Vector product License Agreement can be found by clicking here.

AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.

pAcGFP1-C2 encodes a green fluorescent protein from Aequorea coerulescens. pAcGFP1-C2 is designed for studies in mammalian systems. This vector allows expression of a protein of interest as a C-terminal fusion to AcGFP1. The fusion vector can also be used as a cotransfection marker since the unmodified vector will express fluorescent protein. The fluorescent protein coding sequence in this construct has been human-codon-optimized for efficient expression and enhanced brightness in mammalian cells.

Notice to purchaser

Components

632470

pAcGFP1-C1 Vector

20 ug

Inquire for Quotation

License Statement

ID Number

Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pAcGFP1-C1 Vector product License Agreement can be found by clicking here.

AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.

pAcGFP1-C1 encodes a green fluorescent protein from Aequorea coerulescens. pAcGFP1-C1 is designed for studies in mammalian systems. This vector allows expression of a protein of interest as a C-terminal fusion to AcGFP1. The fusion vector can also be used as a cotransfection marker since the unmodified vector will express fluorescent protein. The fluorescent protein coding sequence in this construct has been human-codon-optimized for efficient expression and enhanced brightness in mammalian cells.

Notice to purchaser

Components

632469

pAcGFP1-N1 Vector

20 ug

Inquire for Quotation

License Statement

ID Number

Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pAcGFP1-N1 Vector product License Agreement can be found by clicking here.

AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.

pAcGFP1-N1 encodes a green fluorescent protein from Aequorea coerulescens. pAcGFP1-N1 is designed for studies in mammalian systems. This vector allows expression of a protein of interest as an N-terminal fusion to AcGFP1. The fusion vector can also be used as a cotransfection marker since the unmodified vector will express fluorescent protein. The fluorescent protein coding sequence in this construct has been human-codon-optimized for efficient expression and enhanced brightness in mammalian cells.

Notice to purchaser

Components

632468

pAcGFP1 Vector

20 ug

Inquire for Quotation

License Statement

ID Number

Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pAcGFP1 Vector product License Agreement can be found by clicking here.

AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.

pAcGFP1 encodes a monomeric green fluorescent protein from Aequorea coerulescens. pAcGFP1 is a prokaryotic expression vector that also serves as a convenient source of fluorescent protein cDNA. In this vector, the full-length human-codon-optimized cDNA is flanked by distinct MCSs to facilitate excision from the vector and insertion into other eukaryotic or prokaryotic expression constructs. The fluorescent protein coding sequence in this construct has been human-codon-optimized for efficient expression and enhanced brightness in mammalian cells.

Notice to purchaser

Components

632464

pAcGFP1-Golgi Vector

20 ug

Inquire for Quotation

License Statement

ID Number

Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pAcGFP1-Golgi Vector product License Agreement can be found by clicking here.

AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.

The pAcGFP1-Golgi Vector encodes a fusion protein consisting of Aequorea coerulescens green fluorescent protein (AcGFP1) fused at its N-terminus to 81 amino acids of the precursor to the human beta 1,4-galactosyltransferase (GT). AcGFP1 protein is optimized for brighter fluorescence and higher expression in mammalian cells. pAcGFP1-Golgi can be used for specific labeling of the trans-medial region of the Golgi apparatus in mammalian cells.

Notice to purchaser

Components

632453

pAcGFP1-Actin Vector

20 ug

Inquire for Quotation

License Statement

ID Number
39	AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.

pAcGFP1-Actin encodes a fusion protein consisting of Aequorea coerulescens green fluorescent protein (AcGFP1) fused at its C-terminus to the human cytoplasmic b-actin. The AcGFP1-Actin fusion protein incorporates into growing actin filaments, allowing visualization of the actin cytoskeleton in living or fixed cells.

Notice to purchaser

Components

632435

pIRES2-AcGFP1 Vector

20 ug

Inquire for Quotation

License Statement

ID Number

Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pIRES2-AcGFP1 Vector product License Agreement can be found by clicking here.

AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.

pIRES2-AcGFP1 is a bicistronic vector designed for the simultaneous expression of a novel green fluorescent protein (AcGFP1) and a protein of interest from the same transcript in transfected mammalian cells. This vector contains an internal ribosome entry site (IRES2) of the encephalomyocarditis virus (EMCV). When a gene of interest is inserted into the MCS located upstream of the IRES, both the gene of interest and the AcGFP1 coding region will be translated from a single bicistronic mRNA.

Notice to purchaser

Components

632432

pAcGFP1-Mito Vector

20 ug

Inquire for Quotation

License Statement

ID Number

Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pAcGFP1-Mito Vector product License Agreement can be found by clicking here.

AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.

pAcGFP1-Mito encodes a humanized green fluorescent protein (GFP) derived from Aequorea coerulescens. A mitochondrial targeting sequence from the precursor protein of human cytochrome c oxidase subunit VIII is fused to the N-terminus of the fluorescent protein. The fluorescence from pAcGFP1-Mito expression can be observed within the mitochondrial matrix inside the inner membrane. pAcGFP1-Mito can be used for specific labeling of mitochondria in living and fixed cells.

Notice to purchaser

Components

632431

pAcGFP1-Nuc Vector

20 ug

Inquire for Quotation

License Statement

ID Number

Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pAcGFP1-Nuc Vector product License Agreement can be found by clicking here.

AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.

pAcGFP1-Nuc encodes a humanized green fluorescent protein (GFP) derived from Aequorea coerulescens. The fluorescent protein is fused at its C-terminus to three copies of the nuclear localization signal (NLS) of the SV40 T-antigen. This vector is designed a marker for visualizing the nucleus in living or fixed cells by fluorescence microscopy.

Notice to purchaser

Components

632426

AcGFP1 Vector Set

Each

Inquire for Quotation

License Statement

ID Number
39	AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.

A set of three vectors encoding green fluorescent protein from Aequorea coerulescens. pAcGFP1 is a prokaryotic expression vector that also serves as a convenient source of fluorescent protein cDNA. In this vector, the full-length human-codon-optimized cDNA is flanked by distinct MCSs to facilitate excision from the vector and insertion into other eukaryotic or prokaryotic expression constructs. The other vectors, pAcGFP1-C1 and pAcGFP1-N1, are designed for studies in mammalian systems. These vectors allow expression of a protein of interest as a C- or N-terminal fusion, respectively. The fusion vectors can also be used as cotransfection markers since the unmodified vectors will express fluorescent protein. All of the fluorescent protein coding sequences in these constructs have been human-codon-optimized for efficient expression in mammalian cells.

Notice to purchaser

Components

632155

pLVX-AcGFP1-C1 Vector

10 ug

Inquire for Quotation

License Statement

ID Number

Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pLVX-AcGFP1-C1 Vector product License Agreement can be found by clicking here.

AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.

The pLVX-AcGFP1-C1 vector is an HIV-1 based lentiviral expression vector that can produce recombinant lentivirus that efficiently transduces both dividing and non-dividing mammalian cells. This vector expresses a variant of the green fluorescent protein (GFP) from Aequorea coerulescens. pLVX-AcGFP1-C1 allows cloning of a cDNA into the multiple cloning site (MCS) downstream of the coding sequence for AcGFP1. The cDNA is subsequently expressed as a fusion to the C-terminus of the AcGFP1 protein. Thus,
this vector allows the expression of fluorescent fusion proteins in difficult-to-transfect cells. It also contains a puromycin selection marker for selection of stable transductants. The unmodified vector will express AcGFP1 protein and may be used to produce marker virus to optimize infection protocols.

Notice to purchaser

Components

632154

pLVX-AcGFP1-N1 Vector

10 ug

Inquire for Quotation

License Statement

ID Number

Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pLVX-AcGFP1-N1 Vector product License Agreement can be found by clicking here.

AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.

The pLVX-AcGFP1-N1 vector is an HIV-1-based lentiviral expression vector that can produce recombinant lentivirus that efficiently transduces both dividing and non-dividing mammalian cells. This vector expresses a variant of the green fluorescent protein (GFP) from Aequorea coerulescens. pLVX-AcGFP1-N1 allows cloning of a cDNA into the multiple cloning site (MCS) upstream of the coding sequence for AcGFP1. The cDNA is subsequently expressed as a fusion to the N-terminus of the AcGFP1 protein. Thus, this vector allows the expression of fluorescent fusion proteins in difficult-to-transfect cells. It also contains a puromycin selection marker for selection of stable transductants. The unmodified vector will express AcGFP1 protein and may be used to produce marker virus to optimize infection protocols.

Notice to purchaser

Components

631984

pLVX-EF1alpha-AcGFP1-C1 Vector

10 ug

Inquire for Quotation

License Statement

ID Number

AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.

Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pLVX-EF1alpha-AcGFP1-C1 Vector product License Agreement can be found by clicking here.

pLVX-EF1α-AcGFP1-C1 is a lentiviral expression vector that can be used to generate high-titer lentivirus for transducing virtually any dividing or nondividing mammalian cell type, including primary and stem cells. The vector allows a gene-of-interest to be fused to the C-terminus of the green fluorescent protein AcGFP1. Expression of the fusion is driven by the human elongation factor 1 alpha (EF1α) promoter, which continues to be constitutively active even after stable integration of the vector into the host cell genome. Stable expression of the fusion allows the monitoring of a variety of cellular processes (such as differentiation in primary or stem cells) without the transgene silencing associated with CMV promoters. In addition, the vector allows efficient flow cytometric detection of stably or transiently transfected mammalian cells expressing AcGFP1 fusions, without time-consuming drug and clonal selection.

Notice to purchaser

Components

631983

pLVX-EF1alpha-AcGFP1-N1 Vector

10 ug

Inquire for Quotation

License Statement

ID Number

AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.

Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pLVX-EF1alpha-AcGFP1-N1 Vector product License Agreement can be found by clicking here.

pLVX-EF1α-AcGFP1-N1 is a lentiviral expression vector that can be used to generate high-titer lentivirus for transducing virtually any dividing or nondividing mammalian cell type, including primary and stem cells. The vector allows a gene-of-interest to be fused to the N-terminus of the green fluorescent protein AcGFP1. Expression of the fusion is driven by the human elongation factor 1 alpha (EF1α) promoter, which continues to be constitutively active even after stable integration of the vector into the host cell genome. Stable expression of the fusion allows the monitoring of a variety of cellular processes (such as differentiation in primary or stem cells) without the transgene silencing associated with CMV promoters. In addition, the vector allows efficient flow cytometric detection of stably or transiently transfected mammalian cells expressing AcGFP1 fusions, without time-consuming drug and clonal selection.

Notice to purchaser

Components

631974

pEF1alpha-AcGFP1-C1 Vector

10 ug

Inquire for Quotation

License Statement

ID Number

AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.

Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pEF1alpha-AcGFP1-C1 Vector product License Agreement can be found by clicking here.

pEF1α-AcGFP1-C1 is a mammalian expression vector that constitutively expresses a protein of interest fused to the C-terminus of the green fluorescent protein AcGFP1, even after stable integration of the vector into the host cell genome. Stable, constitutive expression of the bicistronic transcript is driven by the human elongation factor 1 alpha (EF1α) promoter, allowing the monitoring of a variety of cellular processes (such as differentiation in primary or stem cells) without the transgene silencing associated with CMV promoters. The unmodified vector can be used to express modified AcGFP1 in mammalian cells.

Notice to purchaser

Components

631973

pEF1alpha-AcGFP1-N1 Vector

10 ug

Inquire for Quotation

License Statement

ID Number

AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.

Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pEF1alpha-AcGFP1-N1 Vector product License Agreement can be found by clicking here.

pEF1α-AcGFP1-N1 is a mammalian expression vector that constitutively expresses a protein of interest fused to the N-terminus of the green fluorescent protein AcGFP1, even after stable integration of the vector into the host cell genome. Stable, constitutive expression of the fusion protein is driven by the human elongation factor 1 alpha (EF1α) promoter, allowing the monitoring of a variety of cellular processes (such as differentiation in primary or stem cells) without the transgene silencing associated with CMV promoters. The unmodified vector can be used to express AcGFP1 in mammalian cells.

Notice to purchaser

Components

632505

pRetroQ-AcGFP1-N1 Vector

20 ug

Inquire for Quotation

License Statement

ID Number
72	Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pRetroQ-AcGFP1-N1 Vector product License Agreement can be found by clicking here.
39	AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.
8	For Research Use Only, Not for Use in Humans.

The pRetroQ-AcGFP1-N1 vector is a high-titer, self-inactivating retroviral expression vector that is optimized to eliminate promoter interference from the upstream LTR in the integrated provirus. This vector expresses a green fluorescent protein (GFP) from Aequorea coerulescens. pRetroQ-AcGFP1-N1 allows cloning of genes into the multiple cloning site (MCS) upstream of the AcGFP1 coding sequence, which are subsequently expressed as fusions to the N-terminus of the AcGFP1 protein. This vector allows the expression of fluorescent fusion proteins in difficult-to-transfect cells. It also contains a puromycin selection marker for selection of stable integrants. The unmodified vector will express AcGFP1 protein and may be used to produce marker virus to optimize infection protocols.

Notice to purchaser

Components

632506

pRetroQ-AcGFP1-C1 Vector

20 ug

Inquire for Quotation

License Statement

ID Number
72	Living Colors Fluorescent Protein Products: Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by e-mail at licensing@takarabio.com. Not-For-Profit Non-Commercial Use License: A copy of the pRetroQ-AcGFP1-C1 Vector product License Agreement can be found by clicking here.
39	AcGFP is covered by U.S. Patent Numbers; 7,432,053, 7,667,016, 7,879,988 and 7,897,726.
8	For Research Use Only, Not for Use in Humans.

The pRetroQ-AcGFP1-C1 vector is a high-titer, self-inactivating retroviral expression vector that is optimized to eliminate promoter interference from the upstream LTR in the integrated provirus. This vector expresses a green fluorescent protein (GFP) from Aequorea coerulescens. pRetroQ-AcGFP1-C1 allows cloning of genes into the multiple cloning site (MCS) downstream of the AcGFP1 coding sequence, which are subsequently expressed as fusions to the C-terminus of the AcGFP1 protein. This vector allows the expression of fluorescent fusion proteins in difficult-to-transfect cells. It also contains a puromycin selection marker for selection of stable integrants. The unmodified vector will express AcGFP1 protein and may be used to produce marker virus to optimize infection protocols.

Notice to purchaser

Components

*You must be logged in to a Purchasing Account in order to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@takarabio.com.

Overview

Monomeric green fluorescent protein
Similar spectral properties to EGFP
AcGFP1 excitation and emission maxima: 475 and 505 nm, respectively
EGFP excitation and emission maxima: 484 and 510 nm, respectively
Lentiviral (pLVX-) and retroviral (pRetroQ-) formats are available
Antibodies are available for detection of AcGFP1

More Information

Applications

Fusions (N or C terminus)
Transfection controls
Dual labeling with DsRed-Monomer fluorescent protein or mCherry fluorescent protein
Bicistronic coexpression with another protein of interest (pIRES- vectors)
Subcellular localization studies

References

Jain, R. K. et al. Oligomerization of green fluorescent protein in the secretory pathway of endocrine cells. Biochem. J. 360, 645–649 (2001).

Chen, Y. et al. Molecular brightness characterization of EGFP in vivo by fluorescence fluctuation spectroscopy. Biophys. J. 82, 133–144 (2002).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Fluorescent protein related links

Takara Bio USA, Inc.
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FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2025 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

Fluorescent protein plasmids

AcGFP1 fluorescent protein

Notice to purchaser

AcGFP1 is a monomeric protein

632515: pIRES2-AcGFP1-Nuc Vector

Map of the fluorescent pIRES2 bicistronic expression vectors

Expression of two proteins from a single mRNA transcript

Notice to purchaser

632511: pAcGFP1-F Vector

AcGFP1 is a monomeric protein

AcGFP1 is ideal for multicolor and fluorescence microscopy applications

Organelles targeted by the subcellular localization vectors

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Notice to purchaser

AcGFP1 is a monomeric protein

AcGFP1 is ideal for multicolor and fluorescence microscopy applications

Organelles targeted by the subcellular localization vectors

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Notice to purchaser

AcGFP1 is a monomeric protein

AcGFP1 is ideal for multicolor and fluorescence microscopy applications

Organelles targeted by the subcellular localization vectors

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Notice to purchaser

Cloning of PCR-amplified alpha-actinin directly into four different In-Fusion Ready prelinearized DsRed-Monomer and AcGFP1 vectors

AcGFP1 is a monomeric protein

Notice to purchaser

Cloning of PCR-amplified alpha-actinin directly into four different In-Fusion Ready prelinearized DsRed-Monomer and AcGFP1 vectors

AcGFP1 is a monomeric protein

Notice to purchaser

Vector map for promoterless fluorescent protein vectors AcGFP1-1, DsRed2-1, DsRed-Express-1, HcRed1-1 and ZsGreen1-1

632497: pAcGFP1-1 Vector

Notice to purchaser

632492: pAcGFP1-Hyg-C1 Vector

Schematic for fluorescent fusion protein vectors

Hygromycin DsRed-Monomer and AcGFP1 expression vectors

AcGFP1 is a monomeric protein

Notice to purchaser

AcGFP1 is a monomeric protein

AcGFP1 is ideal for multicolor and fluorescence microscopy applications

Organelles targeted by the subcellular localization vectors

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Notice to purchaser

632490: pAcGFP1-Endo Vector

AcGFP1 is a monomeric protein

AcGFP1 is ideal for multicolor and fluorescence microscopy applications

Organelles targeted by the subcellular localization vectors

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Notice to purchaser

Schematic for fluorescent fusion protein vectors

Hygromycin DsRed-Monomer and AcGFP1 expression vectors

Use of AcGFP1 for fusions and fluorescence microscopy applications

AcGFP1 is a monomeric protein

Notice to purchaser

AcGFP1 is a monomeric protein

AcGFP1 is ideal for multicolor and fluorescence microscopy applications

Organelles targeted by the subcellular localization vectors

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Notice to purchaser

Schematic for fluorescent fusion protein vectors

Hygromycin DsRed-Monomer and AcGFP1 expression vectors

Use of AcGFP1 for fusions and fluorescence microscopy applications

AcGFP1 is a monomeric protein

Fluorescence excitation and emission spectra of DsRed-Monomer and AcGFP1

Notice to purchaser

Schematic for fluorescent fusion protein vectors

Hygromycin DsRed-Monomer and AcGFP1 expression vectors

Use of AcGFP1 for fusions and fluorescence microscopy applications

Fluorescence excitation and emission spectra of DsRed-Monomer and AcGFP1

632484: pAcGFP1-N3 Vector

AcGFP1 is a monomeric protein

Notice to purchaser

632483: pAcGFP1-N2 Vector

Schematic for fluorescent fusion protein vectors

Hygromycin DsRed-Monomer and AcGFP1 expression vectors

Use of AcGFP1 for fusions and fluorescence microscopy applications

Fluorescence excitation and emission spectra of DsRed-Monomer and AcGFP1

AcGFP1 is a monomeric protein

Notice to purchaser

632482: pAcGFP1-C3 Vector