AcGFP1 fluorescent protein
AcGFP1 (Aequorea coerulescens GFP) is a monomeric green fluorescent protein with spectral properties similar to those of EGFP. Although AcGFP1 and EGFP sequences have 94% homology at the amino acid level and equivalent brightness, AcGFP1 is a superior alternative for fusion applications because it is a monomer. In contrast, the Aequorea victoria GFP and EGFP form dimers (Jain et al. 2001, Chen et al. 2002). The monomeric nature of AcGFP1 has been confirmed by FPLC gel filtration chromatography, sucrose density gradient ultracentrifugation, and pseudonative gel electrophoresis. (Click the Image Data button in the product table below to view data.)
AcGFP1 (Aequorea coerulescens GFP) is a monomeric green fluorescent protein with spectral properties similar to those of EGFP. Although AcGFP1 and EGFP sequences have 94% homology at the amino acid level and equivalent brightness, AcGFP1 is a superior alternative for fusion applications because it is a monomer. In contrast, the Aequorea victoria GFP and EGFP form dimers (Jain et al. 2001, Chen et al. 2002). The monomeric nature of AcGFP1 has been confirmed by FPLC gel filtration chromatography, sucrose density gradient ultracentrifugation, and pseudonative gel electrophoresis. (Click the Image Data button in the product table below to view data.)
The AcGFP1 fluorescent protein is very stable, allowing you to perform studies over an extended period of time. AcGFP1 can be used to visualize and measure gene expression in a variety of organisms from bacteria to higher plants and animals.
Overview
- Monomeric green fluorescent protein
- Similar spectral properties to EGFP
- AcGFP1 excitation and emission maxima: 475 and 505 nm, respectively
- EGFP excitation and emission maxima: 484 and 510 nm, respectively
- Lentiviral (pLVX-) and retroviral (pRetroQ-) formats are available
- Antibodies are available for detection of AcGFP1
More Information
Applications
- Fusions (N or C terminus)
- Transfection controls
- Dual labeling with DsRed-Monomer fluorescent protein or mCherry fluorescent protein
- Bicistronic coexpression with another protein of interest (pIRES- vectors)
- Subcellular localization studies
References
Jain, R. K. et al. Oligomerization of green fluorescent protein in the secretory pathway of endocrine cells. Biochem. J. 360, 645–649 (2001).
Chen, Y. et al. Molecular brightness characterization of EGFP in vivo by fluorescence fluctuation spectroscopy. Biophys. J. 82, 133–144 (2002).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Fluorescent protein related links
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