NucleoMag DNA FFPE
The NucleoMag DNA FFPE kit is designed for high-throughput DNA purification from formalin-fixed, paraffin-embedded samples. Paraffin is first removed from the sample using Paraffin Dissolver. Tissue samples are then solubilized using a Proteinase K digestion to release DNA into solution. Nucleic acids are then bound to the paramagnetic beads via addition of NucleoMag beads and binding buffer to the lysate. After magnetic separation the NucleoMag beads are washed twice using two different wash buffers to remove contaminants and salts. Pure genomic DNA is finally eluted under low ionic strength conditions in a slightly alkaline elution buffer.
Dissolution of paraffin and lysis of tissue samples are accomplished simultaneously with the Paraffin Dissolver. Following tissue solubilization and decrosslinking via Proteinase K digestion, DNA is bound to NucleoMag paramagnetic beads under appropriate buffer conditions, washed with two separate wash buffers to remove contaminants and salts, and high-purity genomic DNA is eluted under low-ionic-strength conditions (Figure 1).
Figure 1. Overview of the NucleoMag DNA FFPE workflow.
Genomic DNA was purified from formalin-fixed, paraffin-embedded mouse lung, liver, and kidney tissue using NucleoMag DNA FFPE (MN) and two competitor kits (A, I) (Figure 2). The NucleoMag DNA FFPE kit achieved higher DNA yields compared to competitors' kits.
Figure 2. Improved DNA yield using NucleoMag DNA FFPE compared to competitors' kits. Quantification of genomic DNA extracted from formalin-fixed, paraffin-embedded mouse tissue sections from lung, liver, and kidney. All extractions were performed in triplicate.
Genomic DNA was purified with NucleoMag DNA FFPE and two competitors' kits, then analyzed with LightCycler PCR and the DyNAmo Capillary SYBR Green qPCR Kit (amplicon size 191 bp) (Figure 3). Competitor A (Panel A) and Competitor I (Panel B) samples show higher CT values compared to NucleoMag DNA FFPE samples, indicating a lower DNA yield from competitors' kits.
Figure 3. PCR performance of DNA extracted using the NucleoMag DNA FFPE kit versus competitors' kits. DNA extracted from formalin-fixed, paraffin-embedded mouse lung, liver, or kidney was isolated using NucleoMag DNA FFPE kit versus kits from Competitor A (Panel A) or Competitor I (Panel B), then subjected to qPCR.
Reliably obtain high-quality genomic DNA from a wide array of sample types.
Speed up your workflow with a unique lysis chemistry that efficiently releases gDNA from cells, tissues, and organs.
Get highly concentrated DNA from small inputs, such as biopsy materials, forensic samples, or FACS-sorted cells.
Avoid tedious centrifugation steps and process up to 24 samples in parallel.
Obtain high-quality cell-free DNA from larger sample volumes.
Capture circulating DNA from low inputs of plasma or serum.
Isolate genomic DNA from whole blood using scalable magnetic-bead technology.
Isolate cell-free DNA from up to 10 ml of plasma using scalable magnetic-bead technology.
Efficiently isolate viral and bacterial nucleic acids from a wide array of inputs.
Automate your workflow for high-throughput isolation of viral nucleic acids from serum or plasma.
Purify DNA from FFPE tissue samples using paramagnetic beads.
High-throughput purification of DNA from FFPE samples in a 96-well format.
Efficiently isolate viral and bacterial nucleic acids from common veterinary samples.
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