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  • ‹ Back to Genomic DNA purification
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High-throughput RNA isolation from FFPE samples High-throughput RNA extraction protocol for FFPE samples
Efficient isolation of genomic DNA from FFPE tissue samples. NucleoMag DNA FFPE
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High-throughput RNA isolation from FFPE samples High-throughput RNA extraction protocol for FFPE samples
Efficient isolation of genomic DNA from FFPE tissue samples. NucleoMag DNA FFPE
automated nucleic acid purification Automate your workflow

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Product overview

NucleoMag DNA FFPE

The NucleoMag DNA FFPE kit is designed for high-throughput DNA purification from formalin-fixed, paraffin-embedded samples. Paraffin is first removed from the sample using Paraffin Dissolver. Tissue samples are then solubilized using a Proteinase K digestion to release DNA into solution. Nucleic acids are then bound to the paramagnetic beads via addition of NucleoMag beads and binding buffer to the lysate. After magnetic separation the NucleoMag beads are washed twice using two different wash buffers to remove contaminants and salts. Pure genomic DNA is finally eluted under low ionic strength conditions in a slightly alkaline elution buffer.

  • Rapid isolation of DNA from formalin-fixed, paraffin-embedded samples
  • Isolation of DNA from fresh and archived FFPE samples
  • Isolation of DNA from specimen or object slides
  • DNA can be used in downstream PCR applications
  • Support protocol for RNA extraction from FFPE samples also available
NucleoMag principle Superior and consistent DNA yield Superb PCR performance

NucleoMag principle  

Dissolution of paraffin and lysis of tissue samples are accomplished simultaneously with the Paraffin Dissolver. Following tissue solubilization and decrosslinking via Proteinase K digestion, DNA is bound to NucleoMag paramagnetic beads under appropriate buffer conditions, washed with two separate wash buffers to remove contaminants and salts, and high-purity genomic DNA is eluted under low-ionic-strength conditions (Figure 1).

Overview of the NucleoMag DNA FFPE workflow

Figure 1. Overview of the NucleoMag DNA FFPE workflow.

Superior and consistent DNA yield  

Genomic DNA was purified from formalin-fixed, paraffin-embedded mouse lung, liver, and kidney tissue using NucleoMag DNA FFPE (MN) and two competitor kits (A, I) (Figure 2). The NucleoMag DNA FFPE kit achieved higher DNA yields compared to competitors' kits.

Improved DNA yield using NucleoMag DNA FFPE compared to competitors' kits

Figure 2. Improved DNA yield using NucleoMag DNA FFPE compared to competitors' kits. Quantification of genomic DNA extracted from formalin-fixed, paraffin-embedded mouse tissue sections from lung, liver, and kidney. All extractions were performed in triplicate.

Superb PCR performance  

Genomic DNA was purified with NucleoMag DNA FFPE and two competitors' kits, then analyzed with LightCycler PCR and the DyNAmo Capillary SYBR Green qPCR Kit (amplicon size 191 bp) (Figure 3). Competitor A (Panel A) and Competitor I (Panel B) samples show higher CT values compared to NucleoMag DNA FFPE samples, indicating a lower DNA yield from competitors' kits.

PCR performance of DNA extracted using the NucleoMag DNA FFPE kit versus competitors' kits

Figure 3. PCR performance of DNA extracted using the NucleoMag DNA FFPE kit versus competitors' kits. DNA extracted from formalin-fixed, paraffin-embedded mouse lung, liver, or kidney was isolated using NucleoMag DNA FFPE kit versus kits from Competitor A (Panel A) or Competitor I (Panel B), then subjected to qPCR.

Related Products

Cat. # Product Size Price License Quantity Details
744320.1 NucleoMag® DNA FFPE 1 x 96 Preps USD $450.00

NucleoMag DNA FFPE kits employ superparamagnetic beads to enable high-throughput DNA purification from formalin-fixed, paraffin-embedded (FFPE) samples using manual or automated processing. The kits can be used to process fresh or archived samples and are compatible with inputs of ≤5 mg of tissue and ≤15 mg of paraffin. Purified DNA fragments typically range in size from 50 bp–5 kbp and are eluted in volumes >25 µl. The kits include blue-colored Paraffin Dissolver for convenient paraffin removal and enable processing of 96 samples in ~2 hours.

Cat. # 744320.1 includes sufficient reagents and materials for processing of 96 samples.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

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Competitor A (A) and competitor I (B) show higher CT values, indicating a lower yield compared to samples purified with NucleoMag DNA FFPE.

Competitor A (A) and competitor I (B) show higher CT values, indicating a lower yield compared to samples purified with NucleoMag DNA FFPE.

NucleoMag DNA FFPE provides better PCR performance than competitor kits. Genomic DNA was purified from FFPE samples using NucleoMag DNA FFPE in comparison with kits from Competitor A and Competitor I and analyzed using a LightCycler PCR system and the DyNAmo Capillary SYBR Green qPCR Kit (amplicon size: 191 bp). qPCR of DNA purified with kits from Competitor A (Panel A) and Competitor I (Panel B) resulted in higher Ct values, indicating lower DNA yields compared to samples purified with NucleoMag DNA FFPE.

Back

The NucleoMag DNA FFPE kit leverages the reversible adsorption of nucleic acids on paramagnetic beads to allow a quick, simple workflow for high-purity genomic DNA from FFPE tissue.

The NucleoMag DNA FFPE kit leverages the reversible adsorption of nucleic acids on paramagnetic beads to allow a quick, simple workflow for high-purity genomic DNA from FFPE tissue.

The NucleoMag DNA FFPE procedure.

Back

The NucleoMag DNA FFPE kit provides higher DNA yields from embedded mouse lung, liver, and kidney sections compared to competitor kits (A, I).

The NucleoMag DNA FFPE kit provides higher DNA yields from embedded mouse lung, liver, and kidney sections compared to competitor kits (A, I).

NucleoMag DNA FFPE provides higher yields than competitor kits. Genomic DNA was purified in triplicate from embedded mouse lung, liver, and kidney tissue sections using NucleoMag DNA FFPE and two competitor kits. The NucleoMag DNA FFPE kit provided higher DNA yields than the competitor kits (A, I).

Back

744320.1: NucleoMag DNA FFPE

744320.1: NucleoMag DNA FFPE
744320.4 NucleoMag® DNA FFPE 4 x 96 Preps USD $1565.00

NucleoMag DNA FFPE kits employ superparamagnetic beads to enable high-throughput DNA purification from formalin-fixed, paraffin-embedded (FFPE) samples using manual or automated processing. The kits can be used to process fresh or archived samples and are compatible with inputs of ≤5 mg of tissue and ≤15 mg of paraffin. Purified DNA fragments typically range in size from 50 bp–5 kbp and are eluted in volumes >25 µl. The kits include blue-colored Paraffin Dissolver for convenient paraffin removal and enable processing of 96 samples in ~2 hours.

Cat. # 744320.4 includes sufficient reagents and materials for processing of 4 x 96 samples.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

Back

Competitor A (A) and competitor I (B) show higher CT values, indicating a lower yield compared to samples purified with NucleoMag DNA FFPE.

Competitor A (A) and competitor I (B) show higher CT values, indicating a lower yield compared to samples purified with NucleoMag DNA FFPE.

NucleoMag DNA FFPE provides better PCR performance than competitor kits. Genomic DNA was purified from FFPE samples using NucleoMag DNA FFPE in comparison with kits from Competitor A and Competitor I and analyzed using a LightCycler PCR system and the DyNAmo Capillary SYBR Green qPCR Kit (amplicon size: 191 bp). qPCR of DNA purified with kits from Competitor A (Panel A) and Competitor I (Panel B) resulted in higher Ct values, indicating lower DNA yields compared to samples purified with NucleoMag DNA FFPE.

Back

The NucleoMag DNA FFPE kit leverages the reversible adsorption of nucleic acids on paramagnetic beads to allow a quick, simple workflow for high-purity genomic DNA from FFPE tissue.

The NucleoMag DNA FFPE kit leverages the reversible adsorption of nucleic acids on paramagnetic beads to allow a quick, simple workflow for high-purity genomic DNA from FFPE tissue.

The NucleoMag DNA FFPE procedure.

Back

The NucleoMag DNA FFPE kit provides higher DNA yields from embedded mouse lung, liver, and kidney sections compared to competitor kits (A, I).

The NucleoMag DNA FFPE kit provides higher DNA yields from embedded mouse lung, liver, and kidney sections compared to competitor kits (A, I).

NucleoMag DNA FFPE provides higher yields than competitor kits. Genomic DNA was purified in triplicate from embedded mouse lung, liver, and kidney tissue sections using NucleoMag DNA FFPE and two competitor kits. The NucleoMag DNA FFPE kit provided higher DNA yields than the competitor kits (A, I).

Back

744320.4: NucleoMag DNA FFPE

744320.4: NucleoMag DNA FFPE


Genomic DNA purification articles

NucleoSpin Tissue

Reliably obtain high-quality genomic DNA from a wide array of sample types.

NucleoSpin DNA RapidLyse

Speed up your workflow with a unique lysis chemistry that efficiently releases gDNA from cells, tissues, and organs.

NucleoSpin Tissue XS

Get highly concentrated DNA from small inputs, such as biopsy materials, forensic samples, or FACS-sorted cells.

NucleoSnap DNA Plasma

Obtain high-quality cell-free DNA from larger sample volumes.

NucleoSpin Plasma XS

Capture circulating DNA from low inputs of plasma or serum.

NucleoMag DNA Plasma

Isolate cell-free DNA from up to 10 ml of plasma using scalable magnetic-bead technology.

NucleoMag Pathogen

Efficiently isolate viral and bacterial nucleic acids from a wide array of inputs.

NucleoMag Virus

Automate your workflow for high-throughput isolation of viral nucleic acids from serum or plasma.

NucleoMag Blood

Isolate genomic DNA from whole blood using scalable magnetic-bead technology.

NucleoBond HMW DNA

The NucleoBond HMW DNA kit enables isolation of high-quality genomic DNA up to 200 kb in size for long read-sequencing applications.

NucleoMag DNA FFPE

Purify DNA from FFPE tissue samples using paramagnetic beads.

NucleoSpin 96 DNA FFPE

High-throughput purification of DNA from FFPE samples in a 96-well format.

NucleoMag DNA Swab

The NucleoMag DNA Swab kit enables high-throughput isolation of genomic DNA from diverse swab samples.

NucleoMag VET

Efficiently isolate viral and bacterial nucleic acids from common veterinary samples.

NucleoMag 384 Plant

Scale up your plant DNA extraction workflow with parallel processing of 384 samples.

NucleoSpin DNA Plasma Midi

Avoid tedious centrifugation steps and process up to 24 samples in parallel.

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Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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